Xirmada Ogaanshaha Kahortagga Jirka ee Dhexdhexaadinta SARS-CoV-2 (ELISA)
【ISTICMAALKA KULA JIRA】
Sars-CoV-2 Dhexdhexaadinta Qalabka Ogaanshaha Kahortagga Jirka waa Qalab Tartan Enzyme-Linked Immunosorbent Assay (ELISA) loogu talagalay ogaanshaha tayada iyo nus-tirada ee wadarta ka-hortagga unugyada SARS-CoV-2 ee serum aadanaha iyo balasmaha. Xirmada Baahinta Kahortagga Jirka ee SARS-CoV-2 waxaa loo isticmaali karaa gargaar ahaan si loo aqoonsado shakhsiyaadka leh falcelinta la qabsiga difaaca ee SARS-CoV-2, taasoo muujineysa caabuq dhawaan ama ka hor. Xirmada ogaanshaha ka hortagga ee SARS-CoV-2 waa in aan loo isticmaalin in lagu baaro caabuqa ba'an ee SARS-CoV-2.
【HORDHAC】
Infekshannada Coronavirus waxay caadi ahaan keenaan jawaab-celin ka-hortagga unugyada. Heerarka is beddelka ee bukaannada COVID-19 waa 50% iyo 100% maalinta 7 iyo 14 ka dib bilaabashada calaamadaha, siday u kala horreeyaan. Si loo soo bandhigo aqoonta, fayraska u dhigma ee ka takhalusaya antibody-ka dhiigga ayaa loo aqoonsan yahay bartilmaameedka go'aaminta waxtarka antibody-ga iyo xoojinta sare ee antibody-ka dhexdhexaadinta waxay muujinaysaa waxtarka ilaalinta sare. Tijaabada Dhexdhexaadinta Dhimista Plaque (PRNT) ayaa loo aqoonsaday inay tahay heerka dahabka ee lagu ogaanayo ka-hortagga unugyada. Si kastaba ha ahaatee, soo saaristeeda hoose iyo baahida sare ee hawlgalka awgeed, PRNT maaha mid wax ku ool ah oo ku saabsan serodiagnosis baaxad weyn iyo qiimaynta tallaalka. Qalabka Baahinta Kahortagga Jirka ee SARS-CoV-2 wuxuu ku saleysan yahay habka Enzyme-Linked Immunosorbent Assay (ELISA), kaas oo ogaan kara ka-hortagga ka-hortagga ka-hortagga ee muunadda dhiigga iyo sidoo kale si gaar ah u gelaya heerarka fiirsashada ee noocan ah antibody.
【Habka Imtixaanka】
1.In tubooyinka kala duwan, aliquot 120μL ee diyaarsan hACE2-HRP Solution.
2.Ku dar 6 μL ee calibrators, shaybaar aan la garanayn, kontaroolada tayada ee tuubo kasta oo si fiican u walaaq.
3.Tranfer 100μL of kasta oo isku dar ah oo loo diyaariyey in tallaabada 2 galay ceelasha microplate u dhigma sida waafaqsan qaabeynta imtixaanka horay loo qorsheeyay.
3. Ku dabool saxanka saxan saxan oo ku dheji 37 ° C 60 daqiiqo.
4.Ka saar saxanka daboolka oo ku dhaq saxanka qiyaas ahaan 300 μL oo ah 1× Wash Solution halkii ceel afar jeer.
5. Taabo saxanka ku dul shukumaan warqad ah si aad uga saarto dareeraha haraaga ah ee ceelasha ka dib dhaqidda tillaabooyinka.
6.Ku dar 100 μL oo TMB ah ceel kasta oo saxanka ku rid mugdi 20 - 25 ° C 20 daqiiqo.
7.Ku dar 50 μL of Stop Solution ceel kasta si aad u joojiso falcelinta.
8. U akhri nuugista akhristaha microplate 450 nm 10 daqiiqo gudahood
