Mayeso a Matenda a Testsea Malungo Ag pf/pv Mayeso a Tri-line

Kufotokozera Kwachidule:

Cholinga:
Kuyezetsa kumeneku kumapereka njira yofulumira komanso yodalirika yodziwira matenda a malungo oyambitsidwa ndiPlasmodium falciparumndiPlasmodium vivax. Imazindikira ma antigen enieni a malungo (monga HRP-2 ya Pf ndi pLDH ya Pv) omwe amapezeka m'magazi panthawi ya matenda.

Zofunika Kwambiri:

Mapangidwe amizere itatu:
- Mayesowa amatha kuzindikira zonse ziwiriPlasmodium falciparum (Pf)ndiPlasmodium vivax (Pv)matenda, okhala ndi mizere yosiyana ya mtundu uliwonse ndi mzere wowongolera kuti ukhale wotsimikiza.
Zotsatira Zachangu:
- Zotsatira zilipo basi15-20 mphindi, kupangitsa kuti ikhale yoyenera kugwiritsidwa ntchito m'munda ndi kuwunika koyang'anira malo omwe ali ndi mwayi wochepa wopezeka ndi ma laboratory.
Kukhudzika Kwambiri ndi Kufotokozera:
- Mayesowa adapangidwa kuti akhale okhudzidwa kwambiri komanso mwachindunji pozindikira ma antigen a malungo, kupereka zotsatira zolondola kuti zithandizire kuwongolera kwachipatala kwa malungo.
Zosavuta Kugwiritsa Ntchito:
- Mayesowa amafunikira maphunziro ochepa kuti achite ndipo ndi oyenera kugwiritsidwa ntchito kumadera akutali kapena makonda opanda zida.

Titumizireni imelo Tsitsani ngati PDF

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Tsatanetsatane wa Zamalonda:

Mtundu wachitsanzo:
- Magazi athunthu (chala kapena venipuncture magazi).
Nthawi Yozindikira:
- 15-20 mphindi(zotsatira ziyenera kutanthauziridwa mkati mwa mphindi 20; zotsatira pambuyo pa nthawiyi ndizosavomerezeka).
Sensitivity ndi Mwatsatanetsatane:
- Kukhudzika:Nthawi zambiri> 90% pozindikira matenda a Pf ndi Pv.
- Mwatsatanetsatane:Nthawi zambiri> 95% pozindikira Pf ndi Pv.
Zosungirako:
- Sungani pakati4°C ndi 30°C, kutali ndi kuwala kwa dzuwa.
- Osaundana.
- Nthawi ya alumali nthawi zambiri imayambiraMiyezi 12 mpaka 24, malinga ndi malangizo a wopanga.
Kutanthauzira kwa Zotsatira:
- Zotsatira Zabwino:
  - Mizere itatu yowonekera:
    1. C (Control) mzere(zikusonyeza kuti mayeso ndi ovomerezeka).
    2. pf mzere(ngati ma antigen a Plasmodium falciparum apezeka).
    3. Pv mzere(ngati ma antigen a Plasmodium vivax apezeka).
    - Kukhalapo kwa mizere ya Pf ndi/kapena Pv kumasonyeza kuti muli ndi matenda a malungo.

Mfundo:

Kuyeza kwa Immunochromatographic:
Kaseti yoyesera imakhala ndi immobilizedma antibodies a monoclonalmakamaka kwa ma antigen a Plasmodium (mwachitsanzo,HRP-2za pf ndipLDHza Pv).

Magazi akagwiritsidwa ntchito poyesa, ngatimalungo antigenZikakhalapo, zimamangiriza ku ma antibodies opangidwa ndi golide omwe ali mu zitsanzo, omwe amasuntha pa membrane yoyeserera ndi capillary action.
Ngati ndiPlasmodium falciparumantigen ikapezeka, mzere wachikuda udzapangika papf mzere.
Ngati ndiPlasmodium vivaxantigen ikapezeka, mzere wachikuda udzapangika paPv mzere.
TheMzere wowongolera (C)zimatsimikizira kuti mayesowo akugwira ntchito bwino komanso akuwonetsa kutsimikizika kwa mayesowo.

Zolemba:

Kupanga	Ndalama	Kufotokozera
IFU	1	/
Kaseti yoyesera	25	Chikwama chilichonse chosindikizidwa chokhala ndi chipangizo chimodzi choyesera ndi desiccant imodzi
M'zigawo diluent	500μL1 chubu 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Dongosolo la dontho	1	/
Nsapato	/	/

Njira Yoyesera:


1. Sambani m'manja	2. Yang'anani zomwe zili m'kati musanayese, phatikizani phukusi, makaseti oyesera, buffer, swab.	3.Ikani chubu chochotsa m'malo ogwirira ntchito.	4.Chotsani chosindikizira chosindikizira cha aluminiyamu kuchokera pamwamba pa chubu chochotsa chomwe chili ndi bafa.

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5.Chotsani swab mosamala popanda kugwira nsonga.Lowetsani nsonga yonse ya swab 2 mpaka 3 cm mumphuno yakumanja.Dziwani kusweka kwa mphuno.Mungathe kumva izi ndi zala zanu polowetsa mphuno kapena fufuzani. izo mumng'ono. Pakani mkati mwa mphuno mukuyenda mozungulira kasanu kwa masekondi osachepera 15,Tsopano tengani swab ya m'mphuno yomweyi ndikuyiyika mumphuno ina.Sungani mkati mwa mphuno mozungulira kasanu kwa masekondi osachepera 15. Chonde chitani mayesowo mwachindunji ndi chitsanzocho ndipo musatero
zisiyeni zitayima.

6.Ikani swab mu chubu chochotsamo. Tembenuzani swab kwa masekondi pafupifupi 10, tembenuzani swab ndi chubu chochotsa, kukanikiza mutu wa swab mkati mwa chubu ndikufinya mbali za chubu kuti mutulutse madzi ambiri. momwe zingathere kuchokera ku swab.


7. Chotsani swab mu phukusi popanda kukhudza padding.	8.Sakanizani bwino pogwedeza pansi pa chubu.Ikani madontho atatu a chitsanzocho molunjika mu chitsime cha kaseti yoyesera.Werengani zotsatira pambuyo pa mphindi khumi ndi zisanu. Zindikirani: Werengani zotsatira mkati mwa mphindi 20. Apo ayi, pempho la mayeso likulimbikitsidwa.

Kutanthauzira kwa Zotsatira: