Kaseti Yoyesera ya SARS-CoV-2 Neutralizing antibody

Pakuwunika kwabwino kwa Matenda a Coronavirus 2019 (2019-nCOV kapena COVID -19) omwe amachepetsa antibody mu seramu yamunthu/plasma/magazi athunthu.

Kwa akatswiri a In Vitro Diagnostic Gwiritsani Ntchito Pokha

【KUFUNA KUGWIRITSA NTCHITO】

Makaseti Oyesa a SARS-CoV-2 Neutralizing antibody ndi ma chromatographic othamanga

immunoassay pakuzindikira kwabwino kwa anti-anti-novel coronavirus matenda a Coronavirus 2019 m'magazi amunthu, seramu, kapena plasma ngati chothandizira pakuwunika kwa anti-novel coronavirus yamunthu yoletsa antibody titer.

Nyama zoyamwitsa.Mtundu γ makamaka umayambitsa matenda a mbalame.CoV imafalikira makamaka kudzera mumadzi otsekemera kapena kudzera mu aerosol ndi madontho. Palinso umboni wosonyeza kuti amatha kupatsirana kudzera munjira ya ndowe.

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, kapena 2019-nCoV) ndi kachilombo ka RNA kopanda magawo. Ndizomwe zimayambitsa matenda a coronavirus 2019 (COVID-19), omwe amapatsirana mwa anthu.

SARS-CoV-2 ili ndi mapuloteni angapo opangidwa kuphatikiza spike (S), envelopu (E), membrane (M) ndi nucleocapsid (N). Mapuloteni a spike (S) ali ndi receptor binding domain (RBD), yomwe imayang'anira kuzindikira cell surface receptor, angiotensin converting enzyme-2 (ACE2). Zapezeka kuti RBD ya SARS-CoV-2 S puloteni imalumikizana mwamphamvu ndi cholandilira cha ACE2 chamunthu chomwe chimatsogolera ku endocytosis m'maselo am'mapapo akuya komanso kubwereza kwa ma virus.

Kutenga kachilombo ka SARS-CoV-2 kumayambitsa kuyankha kwa chitetezo chamthupi, komwe kumaphatikizapo kupanga ma antibodies m'magazi. Ma antibodies obisika amapereka chitetezo ku matenda am'tsogolo kuchokera ku ma virus, chifukwa amakhalabe m'miyezi yambiri mpaka zaka atatenga kachilomboka ndipo amamanga mwachangu komanso mwamphamvu kwa tizilombo toyambitsa matenda kuti aletse kulowetsedwa kwa ma cell ndi kubwereza. Ma antibodies awa amatchedwa ma antibodies a neutralizing.

【 KUSONGA ZINTHU NDI KUKONZEKERA 】

1.Kaseti ya SARS-CoV-2 Neutralizing antibody Test Cassette idapangidwa kuti igwiritsidwe ntchito ndi magazi athunthu, seramu kapena plasma zokha.

2.Zitsanzo zomveka bwino, zopanda hemolyzed zimalimbikitsidwa kuti zigwiritsidwe ntchito ndi mayesowa. Seramu kapena plasma iyenera kulekanitsidwa posachedwa kuti mupewe hemolysis.

3.Yesetsani kuyesa mwamsanga mutatha kusonkhanitsa zitsanzo. Osasiya zitsanzo pa kutentha kwapakati kwa nthawi yayitali. Zitsanzo za seramu ndi plasma zimatha kusungidwa pa 2-8 ° C kwa masiku atatu. Pofuna kusungirako nthawi yayitali, zitsanzo za seramu kapena plasma ziyenera kusungidwa pansi pa 20 ° C. Magazi onse omwe amatengedwa ndi venipuncture ayenera kusungidwa pa 2-8 ° C ngati mayesero ayenera kuchitidwa mkati mwa masiku 2 mutatolera. zitsanzo. Magazi athunthu otengedwa ndi chala ayesedwe msanga.

4.Mitsuko yomwe ili ndi anticoagulants monga EDTA, citrate, kapena heparin iyenera kugwiritsidwa ntchito posungira magazi athunthu. Bweretsani zitsanzo ku kutentha kwa chipinda musanayambe kuyesa.

5. Zitsanzo zozizira ziyenera kusungunuka ndi kusakaniza bwino musanayesedwe. Pewani kuzizira mobwerezabwereza

ndi kusungunuka kwa zitsanzo.

6.Ngati zitsanzo ziyenera kutumizidwa, zinyamuleni motsatira malamulo onse oyendetsera ntchito.

mankhwala a etiological.

7.Icteric, lipemic, hemolyzed, kutentha mankhwala ndi sera zoipitsidwa zingayambitse zotsatira zolakwika.

8. Mukasonkhanitsa magazi a ndodo ya chala ndi lancet ndi pad ya mowa, Chonde tayani dontho loyamba la

magazi athunthu.

1. Bweretsani thumba kuti lizizizira kwambiri musanatsegule. Chotsani chipangizo choyesera m'thumba lomata ndipo chigwiritseni ntchito mwamsanga.

2. Ikani chipangizo choyesera pamalo oyera komanso osakanikirana.

Kwa Zitsanzo za Seramu kapena Plasma: Pogwiritsa ntchito Micropipette, ndi kusamutsa 5ul seramu / plasma ku chitsime cha chitsanzo cha chipangizo choyesera, kenaka yikani dontho la 2 la bafa, ndikuyamba chowerengera.

Za Magazi Athunthu (Venipuncture/Fingerstick) Zitsanzo: Lanya chala chako ndikufinya chala chako pang'onopang'ono, gwiritsani ntchito pipette ya pulasitiki yoperekedwa kuti uyamwe 10ul ya magazi athunthu mpaka 10ul ya pipette ya pulasitiki yotayika, ndikusamutsira ku dzenje lachitsanzo la chipangizo choyesera (ngati kuchuluka kwa magazi kupitilira chizindikirocho, Chonde tulutsani magazi ochulukirapo mu pipette), kenaka onjezani dontho la 2 la buffer, ndikuyambitsa chowerengera. Zindikirani: Zitsanzo zitha kugwiritsidwanso ntchito pogwiritsa ntchito micropipette.

3. Dikirani kuti mizere yamitundu iwonekere. Werengani zotsatira pakadutsa mphindi 15. Osamasulira zotsatira pakadutsa mphindi 20.