SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)
【ZOYENERA KUGWIRITSA NTCHITO】
SARS-CoV-2 Neutralizing Antibody Detection Kit ndi Competitive Enzyme-Linked Immunosorbent Assay (ELISA) yopangidwira kuti izindikire bwino komanso mwapang'onopang'ono ma antibodies oletsa kuphatikizika kwa SARS-CoV-2 mu seramu yamunthu ndi plasma. The SARS-CoV-2 Neutralizing Antibody Detection Kit ingagwiritsidwe ntchito ngati chothandizira kuzindikira anthu omwe ali ndi vuto la chitetezo chamthupi ku SARS-CoV-2, kuwonetsa matenda aposachedwa kapena am'mbuyomu. SARS-CoV-2 Neutralizing Antibody Detection Kit siyenera kugwiritsidwa ntchito pozindikira matenda owopsa a SARS-CoV-2.
【MAU OYAMBA】
Matenda a Coronavirus nthawi zambiri amapangitsa kuti ma antibody asayankhe. Ma seroconversion mwa odwala a COVID-19 ndi 50% ndi 100% patsiku la 7 ndi 14 poyambira zizindikiro, motsatana. Kuti tipereke chidziwitso, ma virus ofananira omwe amalepheretsa ma antibody m'magazi amazindikiridwa ngati chandamale chodziwira mphamvu ya ma antibody komanso kuchuluka kwa ma anti antibody omwe amawonetsa chitetezo chokwanira. Mayeso a Plaque Reduction Neutralization Test (PRNT) akhala akudziwika ngati mulingo wagolide wozindikira ma antibodies omwe amachepetsa. Komabe, chifukwa cha kuchepa kwake komanso kufunikira kwake kwapang'onopang'ono, PRNT siyothandiza pakuwunika kwa serodiagnosis ndi kuwunika kwa katemera. SARS-CoV-2 Neutralizing Antibody Detection Kit idakhazikitsidwa ndi njira ya Competitive Enzyme-Linked Immunosorbent Assay (ELISA), yomwe imatha kuzindikira ma anti-antibody omwe ali m'magazi komanso kupeza makamaka milingo yamtundu wamtundu uwu.
【Njira Yoyesera】
1.Mumachubu osiyana, aliquot 120μL ya HACE2-HRP Solution yokonzedwa.
2.Onjezani 6 μL ya ma calibrators, zitsanzo zosadziwika, zowongolera zamtundu uliwonse mu chubu ndikusakaniza bwino.
3.Transfer 100μL ya kusakaniza kulikonse kokonzekera mu sitepe 2 mu zitsime zofananira za microplate molingana ndi kasinthidwe kokonzedweratu.
3. Phimbani mbale ndi Plate Sealer ndipo muyimire pa 37 ° C kwa mphindi 60.
4.Chotsani Plate Sealer ndikutsuka mbale ndi pafupifupi 300 μL ya 1 × Sambani Njira pa chitsime kwa nthawi zinayi.
5.Dinani mbale papepala chopukutira kuti muchotse madzi otsalira m'zitsime mutatsuka masitepe.
6.Onjezani 100 μL ya TMB Solution pachitsime chilichonse ndikuyika mbale mumdima pa 20 - 25 ° C kwa mphindi 20.
7.Onjezani 50 μL ya Stop Solution pachitsime chilichonse kuti muyimitse zomwe zikuchitika.
8. Werengani kuyamwa kwa microplate reader pa 450 nm mkati mwa mphindi 10 (630nm monga chowonjezera chikulimbikitsidwa kuti chigwire ntchito bwino kwambiri.
