Monkeypox Virus (MPV) Nucleic Acid Detection Kit
MAU OYAMBA
Chidachi chimagwiritsidwa ntchito pozindikira kuti ali ndi kachilombo ka Monkeypox Virus (MPV), milandu yophatikizika ndi zina zomwe zikuyenera kupezeka kuti ali ndi kachilombo ka Monkeypox Virus.
Chidachi chimagwiritsidwa ntchito kuzindikira f3L jini ya MPV paziwopsezo zapakhosi ndi zitsanzo zapamphuno.
Zotsatira zoyezetsa za zidazi ndizongofotokozera zachipatala zokha ndipo siziyenera kugwiritsidwa ntchito ngati njira yokhayo yodziwira matenda.Ndibwino kuti mufufuze mozama za chikhalidwecho potengera matenda a wodwalayo
mawonetseredwe ndi mayesero ena ma laboratory.
Cholinga Chogwiritsidwa Ntchito
| Mtundu woyeserera | zilonda zapakhosi ndi mphuno |
| Mtundu woyesera | Khalidwe |
| Zida zoyesera | PCR |
| Kukula kwa paketi | 48 mayeso / 1 bokosi |
| Kutentha kosungirako | 2-30 ℃ |
| Alumali moyo | 10 miyezi |
PRODUCT NKHANI
Mfundo yofunika
Chidachi chimatenga njira yotetezedwa yamtundu wa MPV f3L monga gawo lomwe mukufuna.Ukadaulo wanthawi yeniyeni wa fluorescence kuchuluka kwa PCR ndi ukadaulo wa nucleic acid wotulutsa mwachangu amagwiritsidwa ntchito kuwunika ma viral nucleic acid kudzera pakusintha kwa siginecha ya fluorescence yazinthu zokulitsa.Njira yodziwikiratu imaphatikizapo kuwongolera kwamtundu wamkati, komwe kumagwiritsidwa ntchito kuyang'anira ngati pali PCR inhibitors mu zitsanzo kapena ngati ma cell omwe ali mu zitsanzo amatengedwa, zomwe zingalepheretse bwino vuto labodza.
ZINTHU ZOFUNIKA KWAMBIRI
Chidacho chili ndi ma reagents opangira mayeso 48 kapena kuwongolera khalidwe, kuphatikiza izi:
Reagent A
| Dzina | Zigawo zazikulu | Kuchuluka |
| Kuzindikira kwa MPV reagent | The reaction chubu ili ndi Mg2+, f3L jini / Rnase P primer kafukufuku, reaction buffer, Taq DNA enzyme. | 48 mayesero |
ReagentB
| Dzina | Zigawo zazikulu | Kuchuluka |
| MPV Kulamulira Kwabwino | Muli ndi MPV chandamale chidutswa | 1 chubu |
| MPV Kuwongolera Koyipa | Popanda chidutswa chandamale cha MPV | 1 chubu |
| DNA yotulutsa reagent | The reagent ili ndi Tris, EDTA ndi Triton. | 48pcs |
| Reconstitution reagent | DEPC mankhwala madzi | 5 ML |
Zindikirani: Zigawo za manambala a batch osiyanasiyana sizingagwiritsidwe ntchito mosiyana
【Zosungirako Zosungira Ndi Moyo Wa alumali】
1.Reagent A / B ikhoza kusungidwa pa 2-30 ° C, ndipo moyo wa alumali ndi miyezi 10.
2.Chonde tsegulani chivundikiro cha chubu choyezetsa pokhapokha mwakonzeka kuyesedwa.
3.Musagwiritse ntchito machubu oyesera kupitirira tsiku lotha ntchito.
4.Musagwiritse ntchito chubu chodziwikiratu chomwe chikutuluka.
【Chida Chogwiritsidwa Ntchito】
Yoyenera pa LC480 PCR kusanthula dongosolo, Gentier 48E Automatic PCR analysis system, ABI7500 PCR analysis system.
【Zitsanzo Zofunika】
1.Zitsanzo zovomerezeka zamitundu: zitsanzo zapakhosi.
2.Sampling yankho:Mukatsimikizira, tikulimbikitsidwa kugwiritsa ntchito chubu ya saline kapena Virus preservation chubu yopangidwa ndi biology ya Hangzhou Testsea potengera zitsanzo.
chifuwa chapakhosi:pukutani matani a pharyngeal ndi khoma lakumbuyo la pharyngeal ndi swab yachitsanzo yotayidwa, kumiza swab mu chubu chokhala ndi 3mL sampling solution, kutaya mchira, ndikumangitsa chivundikiro cha chubu.
3.Sample yosungirako ndi kutumiza:Zitsanzo zomwe ziyenera kuyesedwa ziyenera kuyesedwa mwamsanga.Kutentha kwamayendedwe kumayenera kusungidwa pa 2 ~ 8 ℃. Zitsanzo zomwe zitha kuyesedwa mkati mwa maola 24 zitha kusungidwa pa 2 ℃ ~ 8 ℃ ndipo ngati zitsanzo sizingayesedwe mkati mwa maola 24, ziyenera kusungidwa zosachepera kapena zofanana. mpaka -70 ℃ (ngati palibe chikhalidwe chosungira cha -70 ℃, ikhoza kusungidwa pa -20 ℃ kwakanthawi), pewani mobwerezabwereza
kuzizira ndi kusungunuka.
4.Kusonkhanitsa zitsanzo zoyenera, kusungirako, ndi zoyendetsa ndizofunikira kwambiri pakuchita kwa mankhwalawa.
【Njira Yoyesera】
1.Sample processing ndi chitsanzo kuwonjezera
1.1 Zitsanzo processing
Mukasakaniza njira yachitsanzo pamwambapa ndi zitsanzo, tengani 30μL yachitsanzo mu chubu chotulutsa DNA ndikusakaniza mofanana.
1.2 Kutsegula
Tengani 20μL ya reconstitution reagent ndi kuwonjezera kwa MPV kuzindikira reagent, onjezani 5μL wa chitsanzo kukonzedwa pamwamba (Kulamulira zabwino ndi zoipa ulamuliro kukonzedwa mogwirizana ndi zitsanzo), kuphimba chubu kapu, centrifuge izo pa 2000rpm kwa 10. masekondi.
2. Kukulitsa kwa PCR
2.1 Kwezani okonzeka PCR mbale / machubu ku fluorescence PCR chida, Kulamulira Negative ndi ulamuliro zabwino adzakhala anapereka kwa mayeso aliyense.
2.2 Fluorescent channel setting:
1) Sankhani njira ya FAM kuti muzindikire MPV;
2) Sankhani njira ya HEX / VIC yowunikira jini yamkati;
3.Kusanthula zotsatira
Khazikitsani mzere woyambira pamwamba pomwe pamapindikira owongolera a fulorosenti.
4.Kuwongolera khalidwe
4.1 Kuwongolera koyipa: Palibe mtengo wa Ct womwe wapezeka mu FAM, HEX/VIC channel, kapena Ct＞40;
4.2 Kuwongolera kwabwino: Mu FAM, HEX/VIC njira, Ct≤40;
4.3 Zomwe zili pamwambazi ziyenera kukhutitsidwa pakuyesa komweko, apo ayi zotsatira zake ndizosavomerezeka ndipo kuyesa kuyenera kubwerezedwa.
【Kudula mtengo】
Chitsanzo chimatengedwa ngati chabwino pamene: Chandamale sequence Ct≤40, The internal control gene Ct≤40.
【Kutanthauzira kwa zotsatira】
Ulamuliro waubwino ukadutsa, ogwiritsa ntchito akuyenera kuyang'ana ngati pali njira yokulirapo yachitsanzo chilichonse panjira ya HEX/VIC, ngati ilipo komanso ndi Ct≤40, zikuwonetsa kuti jini yowongolera mkati yakulitsidwa bwino ndipo mayesowa ndi ovomerezeka.Ogwiritsa ntchito atha kupitilira kusanthula kotsatira:
3.Pazitsanzo zokulitsa jini yowongolera mkati mwalephera (HEX/VIC
njira, Ct＞40, kapena palibe mapindikidwe okulitsa), kuchuluka kwa ma virus kapena kukhalapo kwa PCR inhibitor kungakhale chifukwa cholepherera, kuyezetsa kuyenera kubwerezedwanso kuchokera pazosonkhanitsira;
4.Kwa zitsanzo zabwino ndi kachilombo koyambitsa matenda, zotsatira za kayendetsedwe ka mkati sizimakhudza;
Pazitsanzo zomwe zapezeka kuti mulibe, zowongolera zamkati ziyenera kuyesedwa kuti zili ndi HIV apo ayi zotsatira zake zonse ndizolakwika ndipo mayesowo akuyenera kubwerezedwa, kuyambira pa sitepe yosonkhanitsa.
Zambiri Zowonetsera
Satifiketi Yolemekezeka
Mbiri Yakampani
Ife, Hangzhou Testsea Biotechnology Co., Ltd ndi kampani yomwe ikukula mwachangu yaukadaulo yazachilengedwe yomwe imagwira ntchito kwambiri pakufufuza, kupanga, kupanga ndi kugawa zida zoyeserera za in-vitro diagnostic(IVD) ndi zida zamankhwala.
Malo athu ndi GMP, ISO9001, ndi ISO13458 ovomerezeka ndipo tili ndi chilolezo cha CE FDA.Tsopano tikuyembekezera kugwirizana ndi makampani ambiri akunja kwa chitukuko.
Timapanga mayeso a chonde, mayeso a matenda opatsirana, kuyesa kugwiritsa ntchito mankhwala osokoneza bongo, zoyeserera za mtima, zoyesa zotupa, kuyesa kwa chakudya ndi chitetezo ndi mayeso a matenda a nyama, kuphatikizanso, mtundu wathu wa TESTSEALABS wadziwika bwino m'misika yapakhomo ndi yakunja.Mitengo yabwino kwambiri komanso mitengo yabwino imatithandiza kutenga 50% ya magawo apakhomo.
Product Process
1.Konzekerani
2.Chophimba
3.Mtanda wodutsa
4. Dulani mzere
5. Msonkhano
6.Pakani matumba
7. Tsekani matumbawo
8.Pakani bokosi
9.Encasement
