Monkeypox Virus (MPV) Nucleic Acid Detection Kit
MAU OYAMBA
Chidachi chimagwiritsidwa ntchito pozindikira kuti ali ndi kachilombo ka Monkeypox Virus (MPV), milandu yophatikizika ndi zina zomwe zikuyenera kupezeka kuti ali ndi kachilombo ka Monkeypox Virus.
Chidachi chimagwiritsidwa ntchito kuzindikira jini ya f3L ya MPV muzamba zapakhosi ndi zitsanzo zapamphuno.
Zotsatira zoyezetsa za zidazi ndizongofotokozera zachipatala zokha ndipo siziyenera kugwiritsidwa ntchito ngati njira yokhayo yodziwira matenda. Ndibwino kuti mufufuze mozama za matendawa potengera matenda a wodwalayo
mawonetseredwe ndi mayesero ena ma laboratory.
Cholinga Chogwiritsidwa Ntchito
| Mtundu woyeserera | zilonda zapakhosi ndi mphuno |
| Mtundu woyesera | Mkhalidwe |
| Zida zoyesera | PCR |
| Kukula kwa paketi | 48 mayeso / 1 bokosi |
| Kutentha kosungirako | 2-30 ℃ |
| Alumali moyo | 10 miyezi |
PRODUCT NKHANI
Mfundo yofunika
Chidachi chimatenga njira yotetezedwa yamtundu wa MPV f3L monga gawo lomwe mukufuna. Ukadaulo wanthawi yeniyeni wa fluorescence kuchuluka kwa PCR ndi ukadaulo wa nucleic acid wotulutsa mwachangu amagwiritsidwa ntchito kuwunika ma viral nucleic acid kudzera pakusintha kwa siginecha ya fluorescence yazinthu zokulitsa. Njira yodziwikiratu imaphatikizapo kuwongolera kwamtundu wamkati, komwe kumagwiritsidwa ntchito kuyang'anira ngati pali PCR inhibitors mu zitsanzo kapena ngati ma cell omwe ali mu zitsanzo amatengedwa, zomwe zingalepheretse bwino vuto labodza.
ZINTHU ZOFUNIKA KWAMBIRI
Chidacho chili ndi ma reagents opangira mayeso 48 kapena kuwongolera khalidwe, kuphatikiza izi:
Reagent A
| Dzina | Zigawo zazikulu | Kuchuluka |
| Kuzindikira kwa MPV reagent | The reaction chubu ili ndi Mg2+, f3L jini / Rnase P primer kafukufuku, reaction buffer, Taq DNA enzyme. | 48 mayesero |
ReagentB
| Dzina | Zigawo zazikulu | Kuchuluka |
| MPV Kulamulira Kwabwino | Muli ndi MPV chandamale chidutswa | 1 chubu |
| MPV Kuwongolera Koyipa | Popanda chidutswa chandamale cha MPV | 1 chubu |
| DNA yotulutsa reagent | The reagent ili ndi Tris, EDTA ndi Triton. | 48pcs |
| Reconstitution reagent | DEPC mankhwala madzi | 5 ML |
Zindikirani: Zigawo za manambala a batch osiyanasiyana sizingagwiritsidwe ntchito mosiyana
【Zosungirako Zosungira Ndi Moyo Wa alumali】
1.Reagent A / B ikhoza kusungidwa pa 2-30 ° C, ndipo moyo wa alumali ndi miyezi 10.
2.Chonde tsegulani chivundikiro cha chubu choyezetsa pokhapokha mwakonzeka kuyesedwa.
3.Musagwiritse ntchito machubu oyesera kupitirira tsiku lotha ntchito.
4.Musagwiritse ntchito chubu chodziwikiratu chomwe chikutuluka.
【Chida Chogwiritsidwa Ntchito】
Yoyenera pa LC480 PCR kusanthula dongosolo, Gentier 48E Automatic PCR analysis system, ABI7500 PCR analysis system.
【Zitsanzo Zofunika】
1.Zitsanzo zovomerezeka zamitundu: zitsanzo zapakhosi.
2.Sampling yankho:Mukatsimikizira, tikulimbikitsidwa kugwiritsa ntchito chubu ya saline kapena Virus preservation chubu yopangidwa ndi biology ya Hangzhou Testsea potengera zitsanzo.
chifuwa chapakhosi:pukutani matani a pharyngeal ndi khoma lakumbuyo la pharyngeal ndi swab yachitsanzo yotayidwa, kumiza swab mu chubu chokhala ndi 3mL sampling solution, kutaya mchira, ndikumangitsa chivundikiro cha chubu.
3.Sample yosungirako ndi kutumiza:Zitsanzo zomwe ziyenera kuyesedwa ziyenera kuyesedwa mwamsanga. Kutentha kwamayendedwe kumayenera kusungidwa pa 2 ~ 8 ℃. Zitsanzo zomwe zitha kuyesedwa mkati mwa maola 24 zitha kusungidwa pa 2 ℃ ~ 8 ℃ ndipo ngati zitsanzo sizingayesedwe mkati mwa maola 24, ziyenera kusungidwa zosachepera kapena zofanana. mpaka -70 ℃ (ngati palibe kusungirako -70 ℃, ikhoza kusungidwa pa -20 ℃ kwakanthawi), pewani mobwerezabwereza
kuzizira ndi kusungunuka.
4.Kusonkhanitsa zitsanzo zoyenera, kusungirako, ndi zoyendetsa ndizofunikira kwambiri pakuchita kwa mankhwalawa.
【Njira Yoyesera】
1.Sample processing ndi chitsanzo kuwonjezera
1.1 Zitsanzo processing
Mukasakaniza njira yachitsanzo pamwambapa ndi zitsanzo, tengani 30μL yachitsanzo mu chubu chotulutsa DNA ndikusakaniza mofanana.
1.2 Kutsegula
Tengani 20μL ya reconstitution reagent ndi kuwonjezera kwa MPV kuzindikira reagent, onjezani 5μL wa chitsanzo kukonzedwa pamwamba (Kulamulira zabwino ndi zoipa ulamuliro kukonzedwa mogwirizana ndi zitsanzo), kuphimba chubu kapu, centrifuge izo pa 2000rpm kwa 10. masekondi.
2. Kukulitsa kwa PCR
2.1 Kwezani mbale / machubu okonzeka a PCR ku chida cha fluorescence PCR, Kuwongolera koyipa ndi kuwongolera kwabwino kudzakhazikitsidwa pamayeso aliwonse.
2.2 Fluorescent channel setting:
1) Sankhani njira ya FAM kuti muzindikire MPV;
2) Sankhani njira ya HEX / VIC yowunikira jini yamkati;
3.Kusanthula zotsatira
Khazikitsani mzere woyambira pamwamba pomwe pamapindikira owongolera a fulorosenti.
4.Kuwongolera khalidwe
4.1 Kuwongolera koyipa: Palibe mtengo wa Ct womwe wapezeka mu FAM, HEX/VIC channel, kapena Ct＞40;
4.2 Kuwongolera kwabwino: Mu FAM, HEX/VIC njira, Ct≤40;
4.3 Zomwe zili pamwambazi ziyenera kukhutitsidwa pakuyesa komweko, apo ayi zotsatira zake ndizosavomerezeka ndipo kuyesa kuyenera kubwerezedwa.
【Kudula mtengo】
Chitsanzo chimatengedwa ngati chabwino pamene: Chandamale sequence Ct≤40, The internal control gene Ct≤40.
【Kutanthauzira kwa zotsatira】
Ulamuliro waubwino ukadutsa, ogwiritsa ntchito akuyenera kuyang'ana ngati pali njira yokulirapo yachitsanzo chilichonse panjira ya HEX/VIC, ngati ilipo komanso ndi Ct≤40, zikuwonetsa kuti jini yowongolera mkati yakulitsidwa bwino ndipo mayesowa ndi ovomerezeka. Ogwiritsa ntchito atha kupitilira kusanthula kotsatira:
3.Pazitsanzo zokulitsa jini yowongolera mkati mwalephera (HEX/VIC
njira, Ct＞40, kapena palibe mapindikidwe okulitsa), kuchuluka kwa ma virus kapena kukhalapo kwa PCR inhibitor kungakhale chifukwa cholepherera, kuyezetsa kuyenera kubwerezedwanso kuchokera pazosonkhanitsira;
4.Kwa zitsanzo zabwino ndi kachilombo koyambitsa matenda, zotsatira za kayendetsedwe ka mkati sizimakhudza;
Pazitsanzo zomwe zapezeka kuti mulibe, zowongolera zamkati ziyenera kuyesedwa kuti zili ndi HIV apo ayi zotsatira zake zonse ndizolakwika ndipo mayesowo akuyenera kubwerezedwa, kuyambira pa sitepe yosonkhanitsa.
Zambiri Zowonetsera
Satifiketi Yolemekezeka
Mbiri Yakampani
Ife, Hangzhou Testsea Biotechnology Co., Ltd ndi kampani yomwe ikukula mwachangu yaukadaulo yazachilengedwe yomwe imagwira ntchito kwambiri pakufufuza, kupanga, kupanga ndi kugawa zida zoyeserera za in-vitro diagnostic(IVD) ndi zida zamankhwala.
Malo athu ndi GMP, ISO9001, ndi ISO13458 ovomerezeka ndipo tili ndi chilolezo cha CE FDA. Tsopano tikuyembekezera kugwirizana ndi makampani ambiri akunja kwa chitukuko.
Timapanga mayeso a chonde, mayeso a matenda opatsirana, kuyesa kugwiritsa ntchito mankhwala osokoneza bongo, kuyesa kwa mtima, zoyesa zotupa, kuyesa kwa chakudya ndi chitetezo ndi mayeso a matenda a nyama, kuphatikizanso, mtundu wathu wa TESTSEALABS wadziwika bwino m'misika yapakhomo ndi yakunja. Mitengo yabwino kwambiri komanso mitengo yabwino imatithandiza kutenga 50% ya magawo apakhomo.
Product Process
1.Konzekerani
2.Chophimba
3. Mtanda wodutsa
4. Dulani mzere
5. Msonkhano
6.Pakani matumba
7. Tsekani matumbawo
8.Pakani bokosi
9.Encasement
Pewani Tsoka Latsopano: Konzekerani Tsopano Pamene Monkeypox Ifalikira
Pa Ogasiti 14, World Health Organisation (WHO) idalengeza kuti mliri wa monkeypox umapanga "Public Health Emergency of International Concern." Aka ndi nthawi yachiwiri WHO yapereka chenjezo lapamwamba kwambiri lokhudzana ndi mliri wa nyani kuyambira Julayi 2022.
Pakadali pano, mliri wa nyani wafalikira kuchokera ku Africa kupita ku Europe ndi Asia, milandu yotsimikizika idanenedwa ku Sweden ndi Pakistan.
Malinga ndi kafukufuku waposachedwa kwambiri wochokera ku Africa CDC, chaka chino, mayiko 12 omwe ali mamembala a African Union anena kuti pali milandu 18,737 ya nyani, kuphatikiza milandu 3,101 yotsimikizika, milandu 15,636 yomwe akuwakayikira, ndi 541 omwe afa, omwe amwalira ndi 2.89%.
01 Kodi Monkepox ndi chiyani?
Monkeypox (MPX) ndi matenda a zoonotic omwe amayamba chifukwa cha kachilombo ka monkeypox. Itha kufalikira kuchokera ku nyama kupita kwa anthu, komanso pakati pa anthu. Zizindikiro zodziwika bwino ndi malungo, zidzolo, ndi lymphadenopathy.
Kachilombo ka nyani kamalowa m'thupi la munthu kudzera mu mucous nembanemba ndi khungu losweka. Zomwe zimayambitsa matenda ndi monga matenda a nyani ndi makoswe omwe ali ndi kachilomboka, anyani, ndi anyani ena omwe sianthu. Pambuyo pa matenda, nthawi yobereketsa ndi masiku 5 mpaka 21, nthawi zambiri masiku 6 mpaka 13.
Ngakhale kuti anthu ambiri amatha kutenga kachilombo ka nyanipox, pali njira ina yodzitetezera ku nyanipox kwa omwe alandira katemera wa nthomba, chifukwa cha kufanana kwa majini ndi antigenic pakati pa mavairasi. Pakali pano, monkeypox imafalikira pakati pa amuna omwe amagonana ndi amuna kudzera mu kugonana, pamene chiopsezo chotenga matenda kwa anthu ambiri chimakhalabe chochepa.
02 Kodi Mliri wa Monkeypox Uyu Ndi Wosiyana Bwanji?
Kuyambira kuchiyambi kwa chaka, vuto lalikulu la kachilombo ka monkeypox, "Clade II," layambitsa kufalikira kwakukulu padziko lonse lapansi. Chodetsa nkhawa, kuchuluka kwa milandu yoyambitsidwa ndi "Clade I," yomwe ndi yowopsa kwambiri komanso yomwe ili ndi chiwopsezo chachikulu cha kufa, ikuchulukirachulukira ndipo zatsimikiziridwa kunja kwa Africa. Kuphatikiza apo, kuyambira Seputembala chaka chatha, mtundu watsopano, wowopsa komanso wopezeka mosavuta, "Clade Ib,” yayamba kufalikira ku Democratic Republic of the Congo.
Chodziwika bwino pa mliriwu ndi chakuti amayi ndi ana osapitirira zaka 15 ndi omwe akhudzidwa kwambiri.
Zambiri zikuwonetsa kuti 70% ya milandu yomwe yanenedwa ndi ya odwala osakwana zaka 15, ndipo mwa omwe amapha, chiwerengerochi chimakwera mpaka 85%. Makamaka,chiwerengero cha imfa kwa ana ndi kuwirikiza kanayi kuposa akuluakulu.
03 Kodi Chiwopsezo Chofalitsa Monkeypox ndi Chiyani?
Chifukwa cha nyengo ya alendo komanso kuyanjana kwamayiko pafupipafupi, chiopsezo chotenga kachilombo ka nyani podutsa malire chitha kuchulukira. Komabe, kachilomboka kamafala makamaka chifukwa cha kukhudzana kwa nthawi yayitali, monga kugonana, kukhudzana ndi khungu, ndi kupuma pafupi kapena kulankhula ndi ena, kotero kuti mphamvu yake yopatsirana kwa munthu ndi munthu imakhala yochepa kwambiri.
04 Momwe Mungapewere Monkeypox?
Pewani kugonana ndi anthu omwe thanzi lawo silikudziwika. Apaulendo akuyenera kusamala za kufalikira kwa nyani m'maiko ndi madera omwe akupita komanso kupewa kukhudzana ndi makoswe ndi anyani.
Ngati munthu ali pachiwopsezo chachikulu, dziyang'anireni thanzi lanu kwa masiku 21 ndikupewa kuyanjana ndi ena. Ngati zizindikiro monga zidzolo, matuza, kapena kutentha thupi zikuwonekera, funsani kuchipatala mwamsanga ndipo mudziwitse dokotala za makhalidwe oyenera.
Ngati wachibale kapena mnzanu wapezeka ndi nyani, chitani njira zodzitetezera, pewani kuyandikira wodwalayo, ndipo musakhudze zinthu zimene wodwalayo wagwiritsa ntchito, monga zovala, zofunda, matawulo, ndi zinthu zina zaumwini. Pewani kugawana zipinda zosambira, ndipo nthawi zambiri muzisamba m'manja ndi zipinda zolowera mpweya.
Monkeypox Diagnostic Reagents
Monkeypox diagnostic reagents amathandizira kutsimikizira kachilomboka pozindikira ma antigen kapena ma antibodies, kupangitsa njira zoyenera zodzipatula komanso zochizira, ndikuchita gawo lofunikira pakuwongolera matenda opatsirana. Pakadali pano, Anhui DeepBlue Medical Technology Co., Ltd. yapanga zowunikira zotsatirazi:
Monkeypox Antigen Test Kit: Imagwiritsa ntchito njira yagolide ya colloidal kusonkhanitsa zitsanzo monga oropharyngeal swabs, nasopharyngeal swabs, kapena zotulutsa pakhungu kuti zizindikire. Imatsimikizira matenda pozindikira kukhalapo kwa ma virus antigen.
Chida Choyeserera cha Monkeypox Antibody: Imagwiritsa ntchito njira yagolide ya colloidal, yokhala ndi zitsanzo kuphatikiza magazi athunthu a venous, plasma, kapena seramu. Imatsimikizira matenda pozindikira ma antibodies opangidwa ndi thupi la munthu kapena nyama motsutsana ndi kachilombo ka monkeypox.
Monkeypox Virus Nucleic Acid Test Kit: Imagwiritsa ntchito njira yeniyeni ya PCR ya fulorosenti, ndipo chitsanzocho chimakhala chotupa cha exudate. Imatsimikizira matenda pozindikira majeremusi a virus kapena tiziduswa ta jini.
Zida Zoyesera za Monkeypox za Testsealabs
Kuyambira 2015, zowunikira za Testsealabs's monkeypox zatsimikiziridwa pogwiritsa ntchito zitsanzo zenizeni zama virus m'ma laboratories akunja ndipo zatsimikiziridwa ndi CE chifukwa chokhazikika komanso chodalirika. Ma reagents awa amayang'ana mitundu yosiyanasiyana yazitsanzo, zopatsa chidwi komanso kutsimikizika kosiyanasiyana, kupereka chithandizo champhamvu pakuzindikira matenda a nyani ndikuthandizira bwino pakuthana ndi mliri. Kuti mudziwe zambiri za zida zathu zoyesera nyani, chonde onaninso: https://www.tesselabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Njira yoyesera
Pogwiritsa ntchito swab kusonkhanitsa mafinya kuchokera ku pustule, kusakaniza bwinobwino mubuffer, ndiyeno kugwiritsa ntchito madontho ochepa mu khadi yoyesera. Zotsatira zake zitha kupezeka m'njira zingapo zosavuta.
