Kaseti Yoyesera ya MonkeyPox Antigen (Seramu/Magazi amadzimadzi/Maswabs)

Kaseti Yoyesera ya MonkeyPox Antigen (Seramu/Magazi a Plasma/Swabs) Chithunzi Chowonetsedwa

Kufotokozera Kwachidule:

Monkeypoxndi ma virus zoonotic matenda chifukwaMonkeypox virus, yomwe ili yaMtundu wa OrthopoxviruschaPoxviridae banja. Ngakhale kuti amafanana ndi nthomba, anyani nthawi zambiri sakhala ovuta kwambiri ndipo amafa ochepa. Kachilomboka kanapezeka koyamba mu1958mu labotale anyani (motero dzina), koma tsopano amadziwika makamaka zimakhudza makoswe ndi nyama zina. Matendawa adanenedwa koyamba mwa anthu1970muDemocratic Republic of Congo.

Monkeypox imatha kufalikira kwa anthu kudzera mu:

Kulumikizana mwachindunjindi nyama zomwe zili ndi kachilombo (mwachitsanzo, kugwira nyama yamtchire).
Kupatsirana kwa munthu ndi munthukudzera m'malovu opumira, kukhudzana kwambiri ndi madzi am'thupi, kapena zotupa pakhungu.
Zinthu zomwe zikuyenera kufalitsa matenda(zinthu zoipitsidwa kapena malo).

Zizindikiro za nyanipox mwa anthu zimafanana ndi zanthomba, kuphatikizapo:

Malungo
Mutu
Kupweteka kwa minofu
Kutopa
Ziphuphu, zomwe zimayambira kumaso ndi kufalikira ku ziwalo zina za thupi, nthawi zambiri zimasanduka zotupa zodzaza madzimadzi (pox).

Titumizireni imelo Tsitsani ngati PDF

Tsatanetsatane wa Zamalonda

Zolemba Zamalonda

Tsatanetsatane wa Zamalonda:

Kuzindikira Kwambiri ndi Kufotokozera
Mayesowa adapangidwa kuti apereke kuzindikira kolondola kwaMonkeypox virus antigens kapena ma antibodies, yokhala ndi ma cross-reactivity ochepa ndi ma virus ena ofanana.
Zotsatira Zachangu
Zotsatira zilipo mkati15-20 mphindi, kuzipangitsa kukhala zabwino popanga zisankho mwachangumakonda azachipatalakapena pa nthawi ya matenda.
Kusavuta Kugwiritsa Ntchito
Mayesowa ndi osavuta kugwiritsa ntchito ndipo safuna maphunziro apadera kapena zida. Ndizoyenera kugwiritsidwa ntchito ndi akatswiri azaumoyo m'malo osiyanasiyana, kuphatikizazipinda zangozi, zipatala zakunja,ndizipatala zakumunda.
Mitundu Yosiyanasiyana ya Zitsanzo
Mayeso amagwirizana ndimagazi athunthu, seramu, kapenaplasma, kupereka kusinthasintha pakusonkhanitsa zitsanzo.
Zonyamula komanso Zoyenera Kugwiritsa Ntchito Kumunda
Mapangidwe ang'onoang'ono a mayesowa amapangitsa kuti ikhale yabwino kugwiritsidwa ntchitomafoni azaumoyo, mapulogalamu ofikira anthu ammudzi,ndizochitika za mliri.

Mfundo:

TheMonkeypox Rapid Test Kitamagwira ntchito pa mfundo yalateral flow immunochromatography, kumene mayeso amazindikira mwinaMa antigen a Monkeypox virus or ma antibodies. Ndondomekoyi ili motere:

Zosonkhanitsa Zitsanzo
Voliyumu yaying'ono yamagazi athunthu, seramu, kapenaplasmaamawonjezedwa pachitsime cha chitsanzo cha chipangizo choyesera. Njira yothetsera vutoli imayikidwa kuti ithandizire kuyenda kwachitsanzo.
Antigen-Antibody Reaction
Kaseti yoyesera ili ndirecombinant antigen or ma antibodieszokhudzana ndi kachilombo ka Monkeypox. Ngati chitsanzocho chili ndi kachilombo ka Monkeypoxma antibodies(IgM, IgG) kapenama antigenkuchokera ku matenda omwe akugwira ntchito, amamanga ku gawo lolingana ndi mzere woyesera.
Kusamuka kwa Chromatographic
Zitsanzo zimayenda motsatira nembanemba chifukwa cha zochita za capillary. Ngati ma antigen kapena ma antibodies enieni a Monkeypox alipo, amamanga pamzere woyesera (T mzere), kupanga gulu lowoneka bwino. Kuyenda kwa ma reagents kumatsimikiziranso kupangidwa kwa amzere wowongolera (C line), zomwe zimatsimikizira kutsimikizika kwa mayeso.
Kutanthauzira zotsatira
- Mizere iwiri (mzere wa T + C):Zotsatira zabwino, kusonyeza kukhalapo kwa Monkeypox virus antigen kapena ma antibodies.
- Mzere umodzi (C mzere wokha):Zotsatira zoyipa, zomwe zikuwonetsa kuti palibe antigen kapena ma antibodies a Monkeypox virus.
- Palibe mzere kapena T mzere wokha:Zotsatira zosalondola, zikufunika kuyesedwanso.

Zolemba:

Kupanga	Ndalama	Kufotokozera
IFU	1	/
Kaseti yoyesera	25	Chikwama chilichonse chosindikizidwa chokhala ndi chipangizo chimodzi choyesera ndi desiccant imodzi
M'zigawo diluent	500μL1 chubu 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Dongosolo la dontho	/	/
Nsapato	25	/

Njira Yoyesera:


1. Sambani m'manja	2. Yang'anani zomwe zili m'kati musanayese, phatikizani phukusi, makaseti oyesera, buffer, swab.	3.Ikani chubu chochotsa m'malo ogwirira ntchito.	4.Chotsani chosindikizira chosindikizira cha aluminiyamu kuchokera pamwamba pa chubu chochotsa chomwe chili ndi bafa.

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5.Chotsani swab mosamala popanda kugwira nsonga.Lowetsani nsonga yonse ya swab 2 mpaka 3 cm mumphuno yakumanja.Dziwani kusweka kwa mphuno.Mungathe kumva izi ndi zala zanu polowetsa mphuno kapena fufuzani. izo mumng'ono. Pakani mkati mwa mphuno mukuyenda mozungulira kasanu kwa masekondi osachepera 15,Tsopano tengani swab ya m'mphuno yomweyi ndikuyiyika mumphuno ina.Sungani mkati mwa mphuno mozungulira kasanu kwa masekondi osachepera 15. Chonde chitani mayesowo mwachindunji ndi chitsanzocho ndipo musatero
zisiyeni zitayima.

6.Ikani swab mu chubu chochotsamo. Tembenuzani swab kwa masekondi pafupifupi 10, tembenuzani swab ndi chubu chochotsa, kukanikiza mutu wa swab mkati mwa chubu ndikufinya mbali za chubu kuti mutulutse madzi ambiri. momwe zingathere kuchokera ku swab.


7. Chotsani swab mu phukusi popanda kukhudza padding.	8.Sakanizani bwino pogwedeza pansi pa chubu.Ikani madontho atatu a chitsanzocho molunjika mu chitsime cha kaseti yoyesera.Werengani zotsatira pambuyo pa mphindi khumi ndi zisanu. Zindikirani: Werengani zotsatira mkati mwa mphindi 20. Apo ayi, pempho la mayeso likulimbikitsidwa.

Kutanthauzira kwa Zotsatira: