ICH-CPV-CDV IgG test kit

CANINE INFECTIOUS HEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ICH/CPV/CDV IgG test kit) idapangidwa kuti iwunikire mochulukirachulukira wagalu wa IgG antibody wa Canine Infectious Hepatitis Virus (ICH), Canine Parvo Virus (CPV) ndi Canine Distemper Virus (CDV).

KONTENTI ZA KIT

CHIPANGIZO NDI MFUNDO

Pali zigawo ziwiri zomwe zimayikidwa mu cartridge iliyonse: Chinsinsi, chomwe chimayikidwa pamodzi ndi desiccant m'chipinda chapansi chosindikizidwa ndi chotchinga choteteza cha aluminiyamu, ndi kupanga njira zothetsera, zomwe zimayikidwa padera m'zipinda zapamwamba zosindikizidwa ndi zojambulazo zoteteza aluminium.

Katiriji iliyonse imakhala ndi ma reagents ofunikira pakuyesa kwachitsanzo chimodzi. Mwachidule, Chinsinsichi chikayikidwa ndi kukulungidwa kwa mphindi zingapo m'chipinda chapamwamba 1, momwe magazi adayikidwa, ma antibodies enieni a IgG omwe ali mumagazi osungunuka, ngati alipo, amamanga ku ICH, CPV kapena CDV recombinant antigens osasunthika pamadontho osiyanasiyana pa Key Key. Kenako Kiyiyo idzasamutsidwa ku zigawo zapamwamba zotsalira pazigawo zokhazikika sitepe ndi sitepe. Ma antibodies okhazikika a IgG pamawangawo adzalembedwa mu chipinda chapamwamba cha 3, chomwe chili ndi anti-canine IgG enzyme conjugate ndipo zotsatira zomaliza zoperekedwa ngati mawanga ofiirira-buluu pa Key zidzapangidwa pamwamba.

chipinda 6, chomwe chili ndi gawo lapansi. Kuti mupeze zotsatira zokhutiritsa, njira zotsuka zimayambitsidwa. M'chipinda chapamwamba 2, IgG yopanda malire ndi zinthu zina zomwe zili mkati mwa magazi zidzachotsedwa. Mu chipinda chapamwamba 4 ndi 5, opanda malire kapena

owonjezera anti-canine IgG enzyme conjugate adzathetsedwa mokwanira. Pamapeto pake, mu chipinda chapamwamba cha 7, chromosome yowonjezereka yopangidwa kuchokera ku gawo lapansi ndi conjugate ya enzyme mu chipinda chapamwamba cha 6 idzachotsedwa. Kuti mutsimikizire kutsimikizika kwa magwiridwe antchito, puloteni yowongolera imayambitsidwa pamalo apamwamba kwambiri pa Key. Malo amtundu wofiirira-buluu ayenera kuwoneka mukamaliza kuyesa kopambana.

KUSINTHA

1. Sungani zida mufiriji wamba (2 ~ 8 ℃).

OSATI KUMUMITSA KITI.

2. Chidacho chili ndi zinthu zachilengedwe zosasinthika. Chidacho chiyenera kugwiridwa

ndi kutayidwa malinga ndi zofunikira zaukhondo.

NJIRA YOYESA

Kukonzekera musanapange mayeso:

1. Bweretsani katiriji kutentha kwa chipinda (20 ℃-30 ℃) ndikuyiyika pa benchi ya ntchito mpaka chizindikiro cha kutentha pa khoma la cartridge chikhale chofiira.

2.Ikani pepala loyera pa benchi ya ntchito kuti muyike Chinsinsi.

3.Konzani choperekera 10μL ndi nsonga za pipette za 10μL.

4. Chotsani zojambulazo za aluminiyamu zoteteza pansi ndikuponya Kiyi kuchokera pansi pa katiriji pa pepala loyera.

5. Imani mowongoka katiriji pa benchi yogwirira ntchito ndikutsimikizira kuti manambala a chipinda chapamwamba amatha kuwoneka molunjika (masimpampu olondola a manambala akukuyang'anani). Dinani katiriji pang'ono kuti muwonetsetse kuti mayankho omwe ali m'zipinda zapamwamba abwerera pansi.

Kuchita mayeso:

1. Tsegulani zojambulazo zotetezera pazipinda zapamwamba mosamala ndi chala chakutsogolo ndi chala chachikulu kuchokera kumanzere kupita kumanja mpaka KUKHALA powonetsa chipinda chapamwamba cha 1.

2.Pezani mayeso a magazi oyesedwa ndi dispenser yokhazikitsidwa pogwiritsa ntchito nsonga ya pipette ya 10μL.

Poyesa seramu kapena plasma gwiritsani ntchito 5μL.

Poyesa magazi athunthu gwiritsani ntchito 10μL.

Machubu a EDTA kapena heparin anticoagulant amalimbikitsidwa kuti azitha kusonkhanitsa magazi athunthu.

3. Ikani chitsanzo mu chipinda chapamwamba 1. Kenaka kwezani ndi kuchepetsa dispenser plunger kangapo kuti mukwaniritse kusakaniza (Kuwala kwa buluu pansonga pamene kusakaniza kumasonyeza kusungitsa bwino chitsanzo).

4.Tengani Kiyi ndi Chogwirizira Keyyo ndi chala chakutsogolo ndi chala chachikulu ndikuyika Kiyiyo mu chipinda chapamwamba 1 (tsimikizirani mbali yachisanu ya Key yomwe ikuyang'anizana nanu, kapena tsimikizirani kuti semi-circle pa Holder ili kumanja mukamayang'ana. inu). Kenako sakanizani ndikuyimitsa Kiyi mu chipinda chapamwamba 1 kwa mphindi zisanu.

5. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka KUKHALA powonetsa chipinda 2. Tengani Kiyi ndi Chogwiritsira ntchito ndikuyika Chinsinsi mu chipinda chowonekera 2. Kenaka sakanizani ndikuyimitsa Chinsinsi mu chipinda chapamwamba 2 kwa mphindi imodzi.

6. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka POKHALA powonetsera chipindacho 3. Tengani Kiyi ndi Chogwirizira ndikuyika Kiyi mu chipinda chowonekera 3. Kenaka sakanizani ndi kuyimitsa Chinsinsi mu chipinda 3 kwa mphindi zisanu.

7. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka KUKHALA kuwonetsa chipinda 4. Tengani Chinsinsi ndi Wogwiritsira ntchito ndikuyika Chinsinsi mu chipinda chowonekera 4. Kenaka sakanizani ndikuyimitsa Chinsinsi mu chipinda chapamwamba cha 4 kwa mphindi imodzi.

8. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka KUKHALA kuwonetsa chipindacho 5. Tengani Chinsinsi ndi Wogwiritsira ntchito ndikuyika Chinsinsi mu chipinda chowonekera 5. Kenaka sakanizani ndikuyimitsa Chinsinsi mu chipinda chapamwamba 5 kwa mphindi imodzi.

9. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka KUKHALA kuwonetsa chipinda 6. Tengani Kiyi ndi Wogwirizira ndikuyika Chinsinsi mu chipinda chowonekera 6. Kenaka sakanizani ndikuyimitsa Chinsinsi mu chipinda chapamwamba 6 kwa mphindi zisanu.

10. Tsegulani zojambulazo zotetezera mosalekeza kumanja mpaka KUKHALA kuwonetsa chipinda 7. Tengani Chinsinsi ndi Wogwiritsira ntchito ndikuyika Chinsinsi mu chipinda chowonekera 7. Kenaka sakanizani ndikuyimitsa Chinsinsi mu chipinda chapamwamba cha 7 kwa mphindi imodzi.

11. Chotsani Kiyi kuchokera mu chipinda chapamwamba 7 ndikusiya kuti chiume papepala kwa mphindi zisanu musanawerenge zotsatira.

Ndemanga:

Osakhudza Frosting Side ya Front End of the Key, komwe ma antigen ndi mapuloteni owongolera samayenda (Chigawo Choyesa ndi Kuwongolera).

Pewani kukanda Chigawo cha Mayeso ndi Kuwongolera potsamira Mbali ina Yosalala ya kumapeto kwa Kiyi ku khoma lamkati la chipinda chilichonse chapamwamba ndikusakaniza.

Pakusakaniza, nthawi 10 kukweza ndi kutsitsa Kiyi mu chipinda chilichonse chapamwamba ndikulimbikitsidwa.

ZOKHA kuwonetsa chipinda chotsatira chapamwamba musanasamutse Kiyi.

Ngati kuli kofunikira, phatikizani Ma Label a Pet omwe aperekedwa kuti muyesere zitsanzo zingapo.

KUMASULIRA ZOPHUNZITSA ZA MAYESERO

Yang'anani madontho otsatila pa Key ndi ColorScale yokhazikika

Zosavomerezeka:

PALIBE mtundu wofiirira wabuluu womwe umawoneka pamalo owongolera

Zoipa(-)

PALIBE mtundu wofiirira wabuluu wooneka pa malo oyesera

Zabwino (+)

Mtundu wowoneka wofiirira-buluu umapezeka pamalo oyesera

Ma titter a ma antibodies enieni a IgG amatha kuwonetsedwa ndi magawo atatu