Ich-CPV-CPV igg mayeso

The Cananine adapatsira matenda opatsirana kwa mankhwalawa / Parvo ndi carnine stavisper virus (CDV).

Zamkati zomwe zilipo

Kapangidwe ndi Mfundo

Pali zinthu ziwiri zomwe zimakwezedwa mu katoni iliyonse: kiyi, yomwe imasungidwa ndi desiccant mu chipinda chotsegulidwa ndi zojambula zoteteza, zomwe zimayikidwa mosiyana ndi zojambulajambula zapamwamba za aluminiyamu.

Makatoni aliwonse amakhala ndi ma reagents onse oyeserera amodzi. Mwachidule, pomwe chinsinsi chake chidayikidwa mu mphindi zochepa mu chipinda chachikulu 1, momwe ma antibodies magazi adayikidwa, igg ya igg mwadzidzidzi, ngati alipo, angamangire ku ICH, CPV kapena CDV kubweza kwa ma argentins overtived pamalo okhazikika pa kiyi yolowera. Kenako fungulo lidzasamutsidwa ku malo otsala omwe ali ndi nthawi pang'ono. Ma antibodies oganiza bwino pamiyalayo adzalembedwera gawo lapamwamba 3, lomwe lili ndi anti-carcine igg enzyme conjugate ndipo zotsatira zomaliza zopangidwa pamwamba

Chuma 6, chomwe chili ndi gawo lapansi. Zotsatira zokhutiritsa, kusamba masitepe kumayambitsidwa. Pa chipinda chapamwamba 2, chisanu ndi zinthu zopanda pake ndi zina zomwe zimachitika munjira yamagazi zidzachotsedwa. Pachipinda chapamwamba 4 ndi 5, chosautsa kapena

Anti-canacine igg enzyme conjugate idzachotsedwa bwino. Kumapeto, m'chipinda chapamwamba 7, chrobosome yowonjezereka kuchokera kudutsa gawo la magawo awiri ndi gawo la Enzyme Conjugate mu chipinda chaposachedwa 6 lidzachotsedwa. Kuti mutsimikizire kuvomerezeka kwa magwiridwe antchito, mapuloteni oletsa kumayambitsidwa pamalo okwera kwambiri pa kiyi. Malo omwe ali mu utoto wofiirira-buluu uyenera kuwoneka mutamaliza kuyeserera kopambana.

Kusunga

1. Sungani To Kit pansi pa firiji (2 ~ 8 ℃).

Osamasula zida.

2. Chidacho chili ndi zinthu zambiri zachilengedwe. Kit iyenera kuchitika

ndi kutayidwa mogwirizana ndi zofunikira zakumaso.

Njira Yoyeserera

Kukonzekera musanayesere mayeso:

1. Bweretsani cartridge kutentha kwa chipinda (20 ℃ -30 ℃) ndikuyiyika pa benchi yantchito mpaka pakhoma la matebulo pakhoma la cartrid amakhala mtundu wofiira.

2. Kuyika pepala loyera pa benchi yogwira ntchito yoyika fungulo.

3.prepare 10μL NAWER ndi 10μl Faipe Maupe.

4. Chotsani zojambula zoteteza a aluminium aluminiyamu ndikutulutsa fungulo kuchokera pa chipinda cha cartridge pa pepala loyera.

5. Yendani zolunjika pa benchi pa benchi ndikutsimikizira kuti manambala apamwamba a centrarment amatha kuwoneka molondola (masitampu olondola omwe akukuyang'anani). Dinani cartridge pang'ono kuti muwonetsetse kuti mayankho omwe ali patsamba lanu amabwerera pansi.

Kuchita mayeso:

1.

2.Konjetsani zitsanzo zoyesedwa ndi magazi ndi diacpenser yokhazikitsidwa pogwiritsa ntchito nsonga ya 10ble.

Poyesa seramu kapena plasma gwiritsani 5μ.

Poyesa kugwiritsidwa ntchito magazi athunthu 10μ.

Edta kapena heparin anticoagulant machubu amalimbikitsidwa kuti plasma ndi kusonkhanitsa magazi.

3. Sungani zitsanzo mu chipinda chachikulu 1. Kenako kwezani ndi kutsitsa kufalikira kangapo kuti mukwaniritse (njira yopepuka ya buluu mu nsonga yomwe ikusakaniza ikuwonetsa chindapusa chopambana).

4 inu). Kenako sakanizani ndikuyimilira kiyi ya chipinda chachikulu 1 kwa mphindi 5.

5. Valani chojambula choteteza mosalekeza mpaka kungotulutsa chipindacho 2. Sankhani kiyi ndi kuyimitsa kiyi ya chipilala chambiri 2 kwa mphindi imodzi.

6. Valani chojambula choteteza mosalekeza mpaka kungotulutsa chipinda chachitatu chokhacho.

7.Cuncover zotchinga zotchinga mosalekeza mpaka kungotulutsa chipindacho 4. Tengani fungulo ndi cholumikizira ndikuyika kiyi imodzi.

8.Cuncover zotchinga zotchinga mosalekeza mpaka kungotsegulira chipindacho 5. Sankhani chinsinsicho ndikuyika chinsinsicho ndikuyika kiyi imodzi yomwe ili pamwamba 5 kwa mphindi imodzi.

9.Cuncover zotchinga zotchinga mosalekeza mpaka kungotulutsa chipindacho 6. Sankhani kiyi ndikuyika chinsinsi cha chipinda chowonekera 6 kwa mphindi 5.

10.Cuncover zotchinga zotchinga mosalekeza mpaka kungotulutsa chipinda chambiri.

11. Tengani fungulo la chipinda chapamwamba 7 ndikuyilola pepala la minofu kwa mphindi 5 musanawerenge zotsatira.

Zolemba:

Osakhudza mbali yozizira ya kumapeto kwa fungulo, komwe ma antigens ndi mapuloteni oletsa ndi osinthika (mayeso ndi owongolera).

Pewani kukanda mayeso ndi kuwongolera kutsatsa mbali ina yosalala ya kumapeto kwa kiyi yopita ku khoma lamkati mwapamwamba pomwe mukusakanikirana.

Kusakanikirana, nthawi 10 kukweza ndikuchepetsa kiyi mu chipinda chilichonse chapamwamba tikulimbikitsidwa.

Ingowonetsa chipinda chotsatira chotsatira musanasanduke fungulo.

Ngati ndi kotheka, gwiritsitsani zolembedwa zomwe zaperekedwa zopitilira muyeso umodzi.

Zotsatira Zosintha

Onani malo omwe adabwezedwawo pa kiyi ndi mtundu wa cortercale

Zosavomerezeka:

Palibe mtundu wowoneka wofiirira wofiirira wowoneka bwino

Wosavomela(-)

Palibe mtundu wowoneka wofiirira wofiirira wofiirira

Zabwino (+)

Mtundu wowoneka wofiirira-buluu umawoneka pamapulogalamu oyeserera

Zabodza za ma antibodies a igg zitha kufanizidwa ndi magawo atatu