【ZOYENERA KUGWIRITSA NTCHITO】

Testsealabs®COVID-19 IgG/IgM Antibody Test Cassette ndi lateral flow chromatographic immunoassay yozindikiritsa ma antibodies a IgG ndi IgM ku COVID-19 m'magazi amunthu, seramu kapena plasma.

【Kufotokozera】

20pc/bokosi (zida zoyesera 20+ machubu 20+1buffer+1 choyikapo)

【ZINTHU ZOPEREKA】

1.Yesani Zipangizo
2.Bafa
3.Madontho
4.Insert Product

【KUSONKHANITSA ZINTHU】

SARS-CoV2 (COVID-19) IgG/IgM AntibodyTest Cassette (Whole Blood/Serum/ Plasma) itha kuchitidwa pogwiritsa ntchito magazi abowo (kuchokera ku venipu kapena ndodo), seramu kapena madzi a m'magazi.

1.Kutolera Zitsanzo za Magazi a Fingerstick Whole Blood:
2.Sambani dzanja la wodwalayo ndi sopo ndi madzi ofunda kapena kuyeretsa ndi swab ya mowa. Lolani kuti ziume.
3.Pasisitani dzanja osakhudza pomwe phunopobokerapo posisita pansi pa chala chapakati kapena chala cha mphete.
4. Phulani khungu ndi lancet wosabala. Chotsani chizindikiro choyamba cha magazi.
5.Pakani dzanja lanu pang'onopang'ono kuchokera pamkono kupita pachikhatho kupita ku chala kuti magazi azikhala ozungulira pamalo oboolapo.
6.Onjezani chitsanzo cha Fingerstick Whole Blood kuyesa pogwiritsa ntchito chubu cha capillary:
7.Kukhudza kumapeto kwa chubu cha capillary mpaka magazi mpaka atadzaza pafupifupi 10mL. Pewani thovu la mpweya.
8.Lekanitsa seramu kapena madzi a m'magazi kuchokera m'magazi mwamsanga kuti mupewe hemolysis. Gwiritsani ntchito zitsanzo zomveka bwino zopanda hemolyzed.

【MMENE MUNGAYESE】

Lolani kuyesa, zitsanzo, zotchingira ndi/kapena zowongolera kuti zifikire kutentha kwachipinda (15-30°C) musanayesedwe.

Chotsani kaseti yoyesera muthumba la zojambulazo ndikuigwiritsa ntchito mkati mwa ola limodzi. Zotsatira zabwino zingapezeke ngati mayesowo achitika mutangotsegula thumba la zojambulazo.
Ikani kaseti pamalo audongo komanso osalala. Kwa zitsanzo za Serum kapena Plasma:

• Kugwiritsa ntchito dontho: Gwirani chotsitsacho molunjika, jambulani chithunzicho pamzere wodzaza (pafupifupi 10mL), ndipo tumizani chitsanzocho kuchitsanzo chabwino (S), kenaka onjezerani madontho awiri a buffer (pafupifupi 80 mL), ndikuyamba chowerengera. .
• Kugwiritsa ntchito pipette: Kusamutsa 10 ml ya chitsanzo ku chitsime cha chitsanzo (S), kenaka yikani madontho awiri a buffer (pafupifupi 80 mL), ndikuyamba chowerengera.

Pachitsanzo cha Magazi Onse a Venipuncture:

• Kugwiritsa ntchito dontho: Gwirani chodonthacho molunjika, jambulani chithunzicho pafupifupi 1 cm pamwamba pa mzere wodzaza ndikusamutsa dontho limodzi lathunthu (pafupifupi 10μL) lachitsanzo kuchitsime (S). Kenako onjezerani madontho awiri a buffer (pafupifupi 80 ml) ndikuyambitsa chowerengera.
• Kugwiritsa ntchito pipette: Kusamutsa 10 ml ya magazi athunthu ku chitsime cha chitsanzo (S), kenaka yikani madontho awiri a buffer (pafupifupi 80 mL), ndikuyamba chowerengera.
• Kwa chitsanzo cha Fingerstick Whole Blood:
• Kugwiritsa ntchito dontho: Gwirani chodonthacho molunjika, jambulani chithunzicho pafupifupi 1 cm pamwamba pa mzere wodzaza ndikusamutsa dontho limodzi lathunthu (pafupifupi 10μL) lachitsanzo kuchitsime (S). Kenako onjezerani madontho awiri a buffer (pafupifupi 80 ml) ndikuyambitsa chowerengera.
• Kugwiritsa ntchito chubu cha capillary: Dzazani chubu cha capillary ndikusamutsa pafupifupi 10mL ya ndodo yamagazi athunthu kupita pachitsime (S) cha makaseti oyesera, kenaka yikani madontho awiri a buffer (pafupifupi 80 mL) ndikuyamba chowerengera. Onani chithunzi pansipa.
• Dikirani kuti mizere yamitundu iwonekere. Werengani zotsatira pa mphindi khumi ndi zisanu. Osamasulira zotsatira pakatha mphindi 20.
• Zindikirani: Ndibwino kuti musagwiritse ntchito buffer, kupitirira miyezi 6 mutatsegula vial.

【KUMASULIRIDWA KWA ZOTSATIRA】

IgG POSITIVE:* Mizere iwiri yamitundu ikuwoneka. Mzere umodzi wachikuda uyenera kuwonekera nthawi zonse m'chigawo cha mzere wowongolera (C) ndipo mzere wina uyenera kukhala m'chigawo cha mzere wa IgG.

IgM POSITIVE:* Mizere iwiri yamitundu ikuwonekera. Mzere umodzi wachikuda uyenera kuwonekera nthawi zonse m'chigawo cha mzere wowongolera (C) ndipo mzere wina uyenera kukhala m'chigawo cha mzere wa IgM.

IgG ndi IgM POSITIVE:* Mizere itatu yamitundu ikuwonekera. Mzere umodzi wachikuda uyenera kuwonekera nthawi zonse m'chigawo cha mzere wowongolera (C) ndipo mizere iwiri yoyeserera iyenera kukhala m'chigawo cha mzere wa IgG ndi mzere wa IgM.

*Dziwani: Kuchulukira kwa utoto m'magawo oyeserera kumatha kusiyanasiyana kutengera kuchuluka kwa ma antibodies a COVID-19 omwe amapezeka pachitsanzocho. Chifukwa chake, mthunzi uliwonse wamtundu mugawo la mzere woyeserera uyenera kuwonedwa ngati wabwino.

ZOSAVUTA: Mzere umodzi wachikuda umapezeka m'chigawo chowongolera (C). Palibe mzere womwe umapezeka m'chigawo cha IgG ndi dera la IgM.

ZOSAVUTA: Mzere wowongolera ukulephera kuwonekera. Kusakwanira kwa kuchuluka kwa zitsanzo kapena njira zolakwika ndizo zifukwa zomwe zimalepheretsa mzere wowongolera. Unikaninso ndondomekoyi poyesa ndi mayeso atsopano. Vuto likapitilira, siyani kugwiritsa ntchito zida zoyeserera nthawi yomweyo ndipo funsani wofalitsa wapafupipafupi.