Ikhasethi Lokuhlola I-Testsealabs Monkey Pox Antigen (Swab)
1.Ikhasethi isetshenziselwa ukuthola ikhwalithi ye-in vitro amacala asolwayo e-Monkeypox Virus (MPV), amacala ahlangene kanye nezinye izimo ezidinga ukuhlolelwa ukutheleleka ngeMonkeypox Virus.
2.I-Cassette iyi-chromatographic immunoassay yokutholwa kwekhwalithi ye-Monkey Pox antigen kuma-oropharyngeal swabs ukusiza ekuxilongeni ukutheleleka ngegciwane leMonkey Pox.
3. Imiphumela yokuhlolwa yaleli Cassette ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengeyona ndlela yodwa yokuxilongwa komtholampilo. Kunconywa ukwenza ukuhlaziya okuphelele kwesimo ngokusekelwe ekubonakalisweni komtholampilo kwesiguli kanye nezinye izivivinyo zaselabhorethri.
ISINGENISO
| Uhlobo lokuhlola | Izifo ze-Oropharyngeal |
| Uhlobo lokuhlola | Ikhwalithi |
| Izinto zokuhlola | Ibhafa yokukhipha esele yapakishwaI-swab oyinyumbaIndawo yokusebenza |
| Usayizi wephakethe | 48 izivivinyo/1 ibhokisi |
| Izinga lokushisa lesitoreji | 4-30°C |
| Impilo yeshelufu | Izinyanga ezingu-10 |
Isici somkhiqizo
Isimiso
I-Monkey Pox Antigen Test Cassette iyi-qualitative membrane strip esekelwe ku-immunoassay ukuze kutholwe i-Monkey Pox antigen kusifanekiso se-oropharyngeal swab. Kule nqubo yokuhlola, i-anti-Monkey Pox antibody ayinyakazi endaweni yomugqa wokuhlola wedivayisi. Ngemuva kokuthi isampula ye-oropharyngeal swab ibekwe esibonelweni kahle, isabela ngezinhlayiya ezimbozwe nge-anti-Monkey Pox antibody ezisetshenziswe kuphedi lesampula. Le ngxube ithutha ngokwechromatographic ngobude bomugqa wokuhlola futhi ihlanganyele ne-anti-Monkey Pox antibody enganyakazi. Uma isifanekiso siqukethe i-Monkey Pox antigen, kuzovela umugqa onemibala endaweni yomugqa wokuhlola obonisa umphumela omuhle.
IZINTO EZIYINHLOKO
Ikhithi iqukethe ama-reagents okucubungula izivivinyo ezingama-48 noma ukulawulwa kwekhwalithi, okuhlanganisa izingxenye ezilandelayo:
①Amasosha omzimba we-Anti-Monkey Pox njengesisetshenziswa esisebenzayo sokuthwebula, elinye i-anti-Monkey Pox antibody njengesisebenzi esitholwayo.
②I-Goat anti-Mouse IgG isetshenziswa ohlelweni lokulawula.
Izimo Zesitoreji Kanye Nempilo Yeshelufu
1.Gcina njengoba kupakishwe esikhwameni esivalekile ekamelweni lokushisa noma esiqandisini (4-30°C)
2.Ukuhlolwa kuzinzile kuze kube yidethi yokuphelelwa yisikhathi ephrintwe esikhwameni esivaliwe. Ukuhlolwa kufanele kuhlale esikhwameni esivaliwe kuze kusetshenziswe.
3.UNGAQISHI. Ungasebenzisi ngale kosuku lokuphelelwa yisikhathi.
Ithuluzi Elisebenzayo
I-Monkey Pox Antigen Test Cassette yakhelwe ukusetshenziswa nama-oropharyngeal swabs.
(Sicela wenze i-swab yenziwe ngumuntu oqeqeshwe ngokwezempilo.)
Izidingo Zesampula
1.Izinhlobo zesampula ezisebenzayo:Izifo ze-Oropharyngeal. Sicela ungabuyiseli i-swab kusisonga saso sokuqala sephepha. Ukuze uthole imiphumela engcono kakhulu, ama-swabs kufanele ahlolwe ngokushesha ngemva kokuqoqwa. Uma kungenakwenzeka ukuhlola ngokushesha, kunjalo
kutuswa kakhulu ukuthi i-swab ifakwe epayipini lepulasitiki elihlanzekile, elingasetshenzisiwe
ebhalwe ngolwazi lwesiguli ukuze kugcinwe ukusebenza kahle kakhulu futhi kugwenywe ukungcoliswa okungenzeka.
2.Isixazululo sesampula:Ngemva kokuqinisekisa, kunconywa ukusebenzisa ithubhu lokulondoloza igciwane elikhiqizwe yi-Hangzhou Testsea biology ukuze kuqoqwe isampula.
3.Isampula yokugcina nokulethwa:Isampula ingagcinwa ivalwe ngokuqinile kuleli shubhu endaweni yokushisa engu-15-30°C) isikhathi esingangehora elilodwa. Qinisekisa ukuthi i-swab ihlezi ngokuqinile ku-tube nokuthi i-cap ivalwe ngokuqinile.
Uma kwenzeka ukubambezeleka okungaphezu kwehora elilodwa, lahla isampula. Kufanele kuthathwe isampula entsha ukuze kuhlolwe. Uma izibonelo zizothuthwa, kufanele zipakishwe ngokuvumelana nemithetho yendawo yokuthutha ama-agent e-atiological.
Indlela Yokuhlola
Vumela ukuhlola, isampula nebhafa ukuthi kufinyelele izinga lokushisa legumbi elingu-15-30°C (59-86°F) ngaphambi kokuqalisa.
① Beka ishubhu yokukhipha endaweni yokusebenza.
② Khipha uphawu lwe-aluminium foil kusukela phezulu kweshubhu yokukhipha equkethe
ithubhu lokukhipha eliqukethe isigcinalwazi sokukhipha.
③ Yenza i-oropharyngeal swab eyenziwa ngumuntu oqeqeshwe ngokwezempilo njenge
kuchaziwe.
④ Faka i-swab kushubhu yokukhipha. Zungezisa i-swab cishe imizuzwana eyi-10
⑤ Khipha i-swab ngokuzungezisa i-vial yokukhipha ngenkathi ucindezela izinhlangothi
webhodlela ukuze ukhulule uketshezi ku-swab.lahla kahle i-swab.ngenkathi ucindezela
ikhanda le-swab ngokumelene nengaphakathi leshubhu yokukhipha ukukhipha uketshezi oluningi
ngangokunokwenzeka kusuka ku-swab.
⑥ Vala ibhodlela ngekepisi enikeziwe bese ucindezela ngokuqinile ku-vial.
⑦ Hlanganisa kahle ngokucwayiza phansi kweshubhu.Beka amathonsi ama-3 esampula
ziqonde efasiteleni lesampula lekhasethi lokuhlola. Funda umphumela ngemva kwemizuzu eyi-10-15. Funda umphumela phakathi nemizuzu engama-20. Uma kungenjalo, kunconywa ukuphindaphinda kokuhlolwa.
Ukuhlaziywa kwemiphumela
1.Okuhle:Kuvela imigqa emibili ebomvu. Umugqa owodwa obomvu uvela endaweni yokulawula (C) kanye nomugqa owodwa obomvu endaweni yokuhlola (T). Ukuhlolwa kubhekwa njengephozithivu uma kuvela ngisho nomugqa omncane. Ukuqina komugqa wokuhlola bungahluka kuye ngokugxila kwezinto ezikhona kusampula.
2.Okubi: Kundawo yokulawula kuphela (C) kuvela umugqa obomvu, endaweni yokuhlola (T) awukho umugqa
liyavela. Umphumela ongemuhle ubonisa ukuthi awekho ama-antigen eMonkeypox kusampula noma ukuhlangana kwama-antigen kungaphansi komkhawulo wokutholwa.
3.Akuvumelekile: Awukho umugqa obomvu ovela endaweni yokulawula (C). Ukuhlola akuvumelekile ngisho noma kunomugqa endaweni yokuhlola (T). Ivolumu yesampula enganele noma ukuphatha okungalungile yizizathu okungenzeka kakhulu zokuhluleka. Buyekeza inqubo yokuhlola bese uphinda ukuhlola ngekhasethi elisha lokuhlola.
Ikhwalithi yokulawula
Ukuhlola kuqukethe umugqa onombala ovela endaweni yokulawula (C) njengesilawuli senqubo yangaphakathi. Iqinisekisa umthamo wesampula owanele kanye nokuphatha okulungile. Amazinga okulawula awanikezwanga nale khithi. Kodwa-ke, kuyanconywa ukuthi izilawuli ezinhle nezingezinhle zihlolwe njengomkhuba omuhle waselabhorethri ukuze kuqinisekiswe inqubo yokuhlola futhi kuqinisekiswe ukusebenza kahle kokuhlolwa.
Izinto eziphazamisayo
Lezi zinhlanganisela ezilandelayo zahlolwa ngokuhlolwa kwe-antigen yeMonkey Pox futhi akukho ukuphazamiseka okuphawuliwe.
