Ukuhlolwa Kwesifo sikamalaleveva i-Tessea Ag pf/pv Ukuhlolwa kwe-Tri-line

Ukuhlolwa Kwesifo sikamalaleveva i-Tessea Ag pf/pv Ukuhlolwa Kwemigqa Emithathu Isithombe Esifakiwe

Incazelo emfushane:

Inhloso:
Lokhu kuhlola kunikeza indlela esheshayo nethembekile yokuhlonza isifo sikamalaleveva esibangelwaI-Plasmodium falciparumfuthiI-Plasmodium vivax. Ithola ama-antigen athile kamalaleveva (afana ne-HRP-2 ye-Pf kanye ne-pLDH ye-Pv) akhona egazini ngesikhathi sokutheleleka okusebenzayo.

Izici Eziyinhloko:

Idizayini yemigqa emithathu:
- Lokhu kuhlola kuyakwazi ukukubona kokubiliI-Plasmodium falciparum (Pf)futhiI-Plasmodium vivax (Pv)izifo, ezinemigqa ehlukene yohlobo ngalunye kanye nomugqa wokulawula wokuqinisekisa ikhwalithi.
Imiphumela esheshayo:
- Imiphumela itholakala nje15-20 imizuzu, okwenza ifanelekele ukusetshenziswa kwenkundla kanye nokuxilongwa kwezindawo zokunakekelwa kwempilo ezindaweni ezingenakho ukufinyelela okulinganiselwe ezindaweni zaselabhorethri.
Ukuzwela Okuphezulu Nokucaciswa:
- Ukuhlolwa kuklanyelwe ukuzwela okuphezulu kanye nokucacisa ekutholeni ama-antigen kamalaleveva, okuhlinzeka ngemiphumela enembile ukusiza ekulawulweni komtholampilo kamalaleveva.
Kulula Ukusebenzisa:
- Ukuhlolwa kudinga ukuqeqeshwa okuncane ukuze kusebenze futhi kulungele ukusetshenziswa ezindaweni ezikude noma izilungiselelo ezinomkhawulo wensiza.

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane Yomkhiqizo:

Uhlobo lwesampula:
- Igazi eliphelele (uthi lweminwe noma isampula yegazi le-venipuncture).
Isikhathi sokutholwa:
- 15-20 imizuzu(imiphumela kufanele ihunyushwe kungakapheli imizuzu engama-20; imiphumela ngemuva kwalesi sikhathi ayivumelekile).
Ukuzwela nokucacisa:
- Ukuzwela:Ngokuvamile> 90% ukuthola kokubili izifo ze-Pf ne-Pv.
- Ukucaciswa:Ngokuvamile > 95% kukho kokubili ukutholwa kwe-Pf ne-Pv.
Imibandela Yesitoreji:
- Gcina phakathi4°C no-30°C, kude nokukhanya kwelanga.
- Ungaqandi.
- Impilo yeshelufu ngokuvamile isukelaIzinyanga eziyi-12 kuye kwezingama-24, kuye ngeziqondiso zomenzi.
Ukuhunyushwa komphumela:
- Umphumela Omuhle:
  - Imigqa emithathu iyabonakala:
    1. C (Lawula) umugqa(ikhombisa ukuthi ukuhlolwa kuvumelekile).
    2. Pf umugqa(uma kutholwa ama-antigen e-Plasmodium falciparum).
    3. Umugqa we-Pv(uma kutholwa ama-antigen e-Plasmodium vivax).
    - Ukuba khona kolayini be-Pf kanye/noma be-Pv kubonisa ukutheleleka ngezinhlobo ezifanele zikamalaleveva.

Isimiso:

Ukuhlolwa kwe-Immunochromatographic:
Ikhasethi lokuhlola liqukethe i-immobilizedama-antibodies e-monoclonalokukhethekile kuma-antigen e-Plasmodium (isb.HRP-2kwe pf kanyepLDHokwe-Pv).

Uma igazi lisetshenziswa ekuhlolweni, umaantigens kamalalevevaakhona, azobophezela kumasosha omzimba ahlanganiswe negolide kusampula, azohamba eduze kolwelwesi lokuhlola ngesenzo se-capillary.
Uma iI-Plasmodium falciparumi-antigen itholakele, kuzokwakhiwa umugqa onemibalaPf umugqa.
Uma iI-Plasmodium vivaxi-antigen itholakele, kuzokwakhiwa umugqa onemibalaUmugqa we-Pv.
IUlayini wokulawula (C)iqinisekisa ukuthi ukuhlola kusebenza kahle futhi kubonisa ukufaneleka kokuhlolwa.

Ukwakhiwa:

Ukwakheka	Inani	Ukucaciswa
IFU	1	/
Hlola ikhasethi	25	Isikhwama ngasinye se-foil esivaliwe esiqukethe idivayisi eyodwa yokuhlola kanye ne-desiccant eyodwa
I-diluent yokukhishwa	500μL1 Ishubhu 25	Ibhafa ye-Tris-Cl, NaCl, NP 40, ProClin 300
Ithiphu ye-Dropper	1	/
I-Swab	/	/

Inqubo Yokuhlola:


1. Geza izandla zakho	2. Hlola okuqukethwe kwekhithi ngaphambi kokuhlolwa, hlanganisa nokufakwa kwephakheji, i-test cassette, i-buffer, i-swab.	3.Beka ishubhu yokukhipha endaweni yokusebenza.	4.Hlubula isivalo se-aluminium foil kusukela phezulu kweshubhu yokukhipha equkethe isigcinalwazi sokukhipha.

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5.Khipha ngokucophelela i-swab ngaphandle kokuthinta ichopho.Faka yonke ichopho le-swab kusuka ku-2 kuya ku-3 cm ekhaleni elingakwesokudla.Qaphela ukuphuka kwe-swab yamakhala.Ungakuzwa lokhu ngeminwe yakho lapho ufaka i-swab yamakhala noma hlola. yona emncane. Gcoba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana engu-15,Manje thatha i-swab yamakhala efanayo futhi uyifake kwenye ikhala.Gwaba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana eyi-15. Sicela wenze ukuhlolwa ngokuqondile nesampula futhi ungakwenzi
ayishiye imile.

6.Beka i-swab eshubhuni yokukhipha. Zungezisa i-swab cishe imizuzwana eyi-10, Zungezisa i-swab eshubhuni lokukhipha, ucindezela ikhanda le-swab phakathi kweshubhu ngenkathi ucindezela izinhlangothi zeshubhu ukuze ukhiphe uketshezi oluningi. ngangokunokwenzeka kusuka ku-swab.


7. Khipha i-swab ephaketheni ngaphandle kokuthinta i-padding.	8.Xuba kahle ngokuqhweba phansi kweshubhu.Beka amathonsi ama-3 esampula ngokuqondile emthonjeni wesampula wekhasethi lokuhlola.Funda umphumela ngemva kwemizuzu engu-15. Qaphela: Funda umphumela kungakapheli imizuzu engama-20. Ngaphandle kwalokho, kunconyelwa ukucela isicelo sokuhlolwa.

Ukuhunyushwa Kwemiphumela: