I-Tessea Disease Test I-HIV 1/2 Ikhithi Yokuhlola Esheshayo

Ukuhlola Isifo Se-Tessea I-HIV 1/2 Ikhithi Yokuhlola Esheshayo Isithombe Esifakiwe

Incazelo emfushane:

I-Human Immunodeficiency Virus (HIV)igciwane elihlasela amasosha omzimba, eliqondise ngqo i-Amaseli e-CD4+ T(aziwa nangokuthi amaseli T-helper), abalulekile ekuvikelweni kwamasosha omzimba. Uma iyekwa ingelashwa, i-HIV ingaholelaI-Acquired Immunodeficiency Syndrome (AIDS), isimo lapho amasosha omzimba alimala kakhulu futhi angakwazi ukulwa nezifo nezifo.

I-HIV isakazwa ngokuyinhlokoigazi, isidoda, uketshezi lwesitho sangasese sowesifazane, uketshezi lwe-rectal, futhiubisi lwebele. Izindlela ezivame kakhulu zokutheleleka zihlanganisa ukuya ocansini okungavikelekile, ukwabelana ngezinaliti ezingcolile, kanye nokudluliselwa kwengane esuka kumama ukuya enganeni ngesikhathi sokubeletha noma encelisa.

Kunezinhlobo ezimbili eziyinhloko ze-HIV:

I-HIV-1:Uhlobo oluvame kakhulu nolusabalele kakhulu lwe-HIV emhlabeni jikelele.
I-HIV-2:Okungavamile, okutholakala kakhulu eNtshonalanga Afrika, futhi ngokuvamile kuhlotshaniswa nokuqhubekela phambili kancane kwe-AIDS.

Ukutholwa kusenesikhathi nokwelashwa ngeUkwelashwa ngezidambisigciwane (ART)ingasiza abantu abane-HIV ukuba baphile isikhathi eside, izimpilo ezinempilo futhi banciphise kakhulu ingozi yokudlulisela kwabanye.

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane Yomkhiqizo:

Ukuzwela Okuphezulu kanye Nokucaciswa
Ukuhlola kuklanyelwe ukuhlonza ngokunembile amasosha omzimba e-HIV-1 kanye ne-HIV-2, kunikeze imiphumela enokwethenjelwa enokusabela okuncane okuphambene.
Imiphumela Esheshayo
Imiphumela itholakala phakathi kwemizuzu engu-15-20, okuvumela ukuthathwa kwezinqumo zomtholampilo ngokushesha kanye nokunciphisa isikhathi sokulinda ezigulini.
Ukusebenziseka kalula
Idizayini elula nesebenziseka kalula, engadingi okokusebenza okukhethekile noma ukuqeqeshwa. Ifanele ukusetshenziswa kuzo zombili izilungiselelo zomtholampilo nasezindaweni ezikude.
Izinhlobo Ezihlukahlukene Zesampula
Ukuhlola kuhambisana negazi eliphelele, i-serum, noma i-plasma, ihlinzeka ngokuguquguquka ekuqoqweni kwesampula nokwandisa ububanzi bezinhlelo zokusebenza.
Ukuphatheka kanye Nesicelo Senkundla
Iqinile futhi ingasindi, okwenza ikhithi yokuhlola ifaneleke kuzilungiselelo zendawo yokunakekela, imitholampilo ephathwayo yezempilo, nezinhlelo zokuhlola abantu abaningi.

Isimiso:

Iqoqo Lesampula
Ivolumu encane ye-serum, i-plasma, noma igazi eliphelele lisetshenziswa emthonjeni wesampula wedivayisi yokuhlola, okulandelwa ukwengezwa kwesixazululo se-buffer ukuqala inqubo yokuhlola.
Ukusebenzisana kwe-Antigen-Antibody
Ukuhlola kuqukethe ama-antigen aphinde ahlanganiswe kokubili i-HIV-1 ne-HIV-2, angakwazi ukuhamba endaweni yokuhlola yolwelwesi. Uma amasosha omzimba e-HIV (IgG, IgM, noma womabili) ekhona kusampula, azobophezela kuma-antigen akulwelwesi, enze i-antigen-antibody complex.
Ukufuduka kweChromatographic
I-antigen-antibody complex ihamba eduze kwe-membrane ngesenzo se-capillary. Uma amasosha omzimba e-HIV ekhona, i-complex izobophezela emugqeni wokuhlola (T line), ikhiqize umugqa onombala obonakalayo. Ama-reagents asele athuthela kulayini wokulawula (umugqa C) ukuze aqinisekise ukufaneleka kokuhlolwa.
Ukuhunyushwa komphumela
- Imigqa emibili (T line + C line):Umphumela omuhle, okhombisa ukuba khona kwamasosha omzimba e-HIV-1 kanye/noma ne-HIV-2.
- Umugqa owodwa (umugqa C kuphela):Umphumela ongemuhle, okhombisa ukuthi awekho amasosha omzimba atholakalayo e-HIV.
- Awukho ulayini noma u-T kuphela:Umphumela ongavumelekile, odinga ukuhlolwa okuphindayo.

Ukwakhiwa:

Ukwakheka	Inani	Ukucaciswa
IFU	1	/
Hlola ikhasethi	1	Isikhwama ngasinye se-foil esivaliwe esiqukethe idivayisi eyodwa yokuhlola kanye ne-desiccant eyodwa
I-diluent yokukhishwa	500μL1 Ishubhu 25	Ibhafa ye-Tris-Cl, NaCl, NP 40, ProClin 300
Ithiphu ye-Dropper	1	/
I-Swab	1	/

Inqubo Yokuhlola:


1. Geza izandla zakho	2. Hlola okuqukethwe kwekhithi ngaphambi kokuhlolwa, hlanganisa nokufakwa kwephakheji, i-test cassette, i-buffer, i-swab.	3.Beka ishubhu yokukhipha endaweni yokusebenza.	4.Hlubula isivalo se-aluminium foil kusukela phezulu kweshubhu yokukhipha equkethe isigcinalwazi sokukhipha.

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5.Khipha ngokucophelela i-swab ngaphandle kokuthinta ichopho.Faka yonke ichopho le-swab kusuka ku-2 kuya ku-3 cm ekhaleni elingakwesokudla.Qaphela ukuphuka kwe-swab yamakhala.Ungakuzwa lokhu ngeminwe yakho lapho ufaka i-swab yamakhala noma hlola. yona emncane. Gcoba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana engu-15,Manje thatha i-swab yamakhala efanayo futhi uyifake kwenye ikhala.Gwaba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana eyi-15. Sicela wenze ukuhlolwa ngokuqondile nesampula futhi ungakwenzi
ayishiye imile.

6.Beka i-swab eshubhuni yokukhipha. Zungezisa i-swab cishe imizuzwana eyi-10, Zungezisa i-swab eshubhuni lokukhipha, ucindezela ikhanda le-swab phakathi kweshubhu ngenkathi ucindezela izinhlangothi zeshubhu ukuze ukhiphe uketshezi oluningi. ngangokunokwenzeka kusuka ku-swab.


7. Khipha i-swab ephaketheni ngaphandle kokuthinta i-padding.	8.Xuba kahle ngokuqhweba phansi kweshubhu.Beka amathonsi ama-3 esampula ngokuqondile emthonjeni wesampula wekhasethi lokuhlola.Funda umphumela ngemva kwemizuzu engu-15. Qaphela: Funda umphumela kungakapheli imizuzu engama-20. Ngaphandle kwalokho, kunconyelwa ukucela isicelo sokuhlolwa.

Ukuhunyushwa Kwemiphumela: