Isifo Sezifo Zesifo Sokuhlola I-HIV 1/2 Ikhithi yokuhlola okusheshayo

Isifo se-TestSea Disease HIV 1/2 Kit okusheshayo kwesithombe esifakiwe

Incazelo emfushane:

I-Human Ammudodeficiency Virus (HIV)yigciwane elihlasela amasosha omzimba, ngokuqondisa ngqoAmaseli we-CD4 + T(eyaziwa nangokuthi amaseli we-T-Helf), abaluleke kakhulu ekuvikeleni ukuzivikela. Uma kushiywe kungalashwa, i-HIV ingaholelaI-ImmunodeFeficielnible Syndrome (AIDS), isimo lapho amasosha omzimba awonakaliswa kakhulu futhi engakwazi ukulwa nezifo kanye nezifo.

Igciwane lesandulela ngculaza lidluliswa ngokuyinhlokoigazi, isidoda, Uketshezi lwangasese, uketshezi lwe-rectal, futhiubisi lwebele. Imizila evame kakhulu yokudluliselwa ifaka ukuxhumana kwezocansi okungavikelekile, yabelana ngezinaliti ezingcolile, kanye nokudluliselwa komama kuya kwengane ngesikhathi sokubeletha noma ukuncelisa.

Kunezinhlobo ezimbili eziyinhloko ze-HIV:

I-HIV-1:Uhlobo oluvame kakhulu futhi olubanzi kakhulu lwe-HIV emhlabeni jikelele.
I-HIV-2:Okujwayelekile, okutholakala ngokuyinhloko eNtshonalanga Afrika, futhi ngokuvamile kuhlotshaniswa nokuthuthuka okuhamba kancane kwengculazi.

Ukutholwa kusenesikhathi nokwelashwa ngeI-Antiretroviral Therapy (ART)Kungasiza abantu abane-HIV baphile impilo ende, enempilo futhi banciphise kakhulu ingozi yokudluliselwa kwabanye.

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane Yomkhiqizo:

Ukuzwela okuphezulu nokucaciswa
Ukuhlolwa kwenzelwe ukuthola kahle amasosha omzimba we-HIV kanye ne-HIV kanye ne-HIV, ukuhlinzeka ngemiphumela ethembekile ngokusezingeni eliphansi.
Imiphumela Esheshayo
Imiphumela iyatholakala kungakapheli imizuzu eyi-15 ukuya kwe-20, inika amandla okwenza izinqumo zokwenziwa komtholampilo kanye nokunciphisa isikhathi sokulinda iziguli.
Ukusebenziseka kalula
Idizayini elula futhi esebenziseka kalula, ayidingi imishini ekhethekile noma ukuqeqeshwa. Ilungele ukusetshenziswa kuzo zombili izilungiselelo zomtholampilo nezindawo ezikude.
Izinhlobo zesampula eziguquguqukayo
Ukuhlolwa kuyahambelana negazi lonke, i-serum, noma i-plasma, ukuhlinzeka ukuguquguquka ekuqoqweni kwesampula nokwandisa uhla lwezicelo.
Ukuthwala kanye nenkundla application
I-compact futhi engasindi, yenza ikhithi yokuhlola ilungele izilungiselelo ze-point-of-care, imitholampilo yezempilo yeselula, nezinhlelo zokuhlola isisindo.

Isimiso:

Ukuqoqwa kwesampula
Ivolumu encane ye-serum, i-plasma, noma igazi eliphelele lisetshenziselwa kahle isampula yedivayisi yokuhlola, elandelwa ukwengeza kwesisombululo se-buffer ukuqala inqubo yokuhlola.
Ukusebenzisana kwe-Antigen-antibody
Ukuhlolwa kuqukethe ama-antigen ahlaziya kabusha ane-HIV-1 ne-HIV-2, engasebenzi esifundeni sokuhlolwa kolwelwesi. Uma ama-antibodies we-HIV (IGG, i-IGM, noma womabili) akhona kusampula, azobopha ama-antigen ku-membrane, akha i-antigen-antibody tandcomple.
Ukufuduka kwe-Chromatographic
I-Antigen-antibody tandandaba ihamba nolwelwesi ngesenzo se-capillary. Uma ama-antibodies we-HIV ekhona, okuyinkimbinkimbi azobopha umugqa wokuhlola (umugqa we-T), akhiqiza umugqa ogqamile onemibala. Ama-reagents asele athuthela kulayini wokulawula (C umugqa) ukuze aqinisekise ukuthi ubuqiniso bokuhlolwa.
Umphumela wokutolika
- Imigqa emibili (i-T Line + C line):Umphumela omuhle, okubonisa ukuba khona kwe-HIV-1 kanye / noma i-HIV-2 antibodies.
- Umugqa owodwa (c umugqa kuphela):Umphumela omubi, ukhombisa ukuthi awekho ama-antibodies atholakala angenayo.
- Awukho umugqa noma umugqa kuphela:Umphumela ongavumelekile, edinga ukuhlolwa okuphindaphindwayo.

Ukwakheka:

Ukwakheka	Inani	Ukucacisa
Uma	1	/
Hlola ikhasethi	1	Isikhwama se-foil ngasinye esivaliwe esine-foil equkethe idivaysi eyodwa yokuhlola ne-desiccant eyodwa
Ukukhishwa okuchithiwe	I-500μL * 1 tube * 25	I-Tris-Cl Buffer, i-NACL, NP 40, Proclin 300
Ithiphu yeCropper	1	/
Celeb	1	/

Inqubo yokuhlola:


1. Geza izandla zakho	2. Bheka okuqukethwe Kit ngaphambi kokuhlola, faka ukufaka iphakheji, i-cassette yokuhlola, i-buffer, i-swab.	3.Phuma ishubhu lokukhishwa endaweni yokusebenza.	I-4PEEL Off Aluminium Foil Faal kusuka phezulu kweshubhu lokukhishwa eliqukethe i-buffer yokukhishwa.

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Ukususa nge-swab ngaphandle kokuthinta ithiphu.Insiza yonke ithiphu ye-swab 2 kuya ku-3 cm ungene endaweni efanelekile ye- Kuku-mimnor. Hlikihla ingaphakathi le-Nostril ekuhambeni okuyindilinga izikhathi ezi-5 okungenani amasekhondi ayi-15, manje thatha i-swab efanayo yamakhala bese uyifaka kwenye inkazimulo ku-tical most amahlandla ama-15. Sicela wenze isivivinyo ngqo ngesampula futhi ungayenzi
shiya kumi.

6.Phumana i-swab e-tube yokukhishwa.Dota i-swab imizuzwana eyi-10, ujikeleze i-swab ngokumelene ne-tube yokukhishwa, ucindezela inhloko ye-swab ngokumelene ne-tube lapho ukhipha izinhlangothi ze-tube ukuze ukhiphe uketshezi oluningi ngangokunokwenzeka kusuka ku-swab.


7. Thatha i-swab kusuka kwiphakheji ngaphandle kokuthinta i-padding.	I-8.mix kahle ngokufaka phansi phansi kwe-tube.place 3 amaconsi empumputhe ebheke kahle kwisampula kahle yekhasethi yokuhlola.Funda umphumela ngemuva kwemizuzu eyi-15. QAPHELA: Funda umphumela kungakapheli imizuzu engama-20.Isitatimende sokuhlola siyanconywa.

Ukuhunyushwa Kwezindlela: