I-SARS-CoV-2 Ikhasethi Lokuhlola I-antibody

Ukuze kuhlolwe ikhwalithi ye-Coronavirus Disease 2019 (2019-nCOV noma i-COVID -19) evimbela ama-antibody ku-serum yomuntu/i-plasma/igazi lonke.

Okokusebenza kwe-In Vitro Diagnostic Kuphela

【UKUSEBENZISA OKUHLOSIWE】

I-SARS-CoV-2 Neutralizing antibody Test Cassette iyi-chromatographic esheshayo

i-immunoassay ukuze kutholwe ikhwalithi yokuqeda amasosha omzimba we-Coronavirus Disease 2019 egazini lomuntu lonke, i-serum, noma i-plasma njengosizo emazingeni okuhlolwa kwe-anti-novel coronavirus yabantu eqeda i-antibody titer.

izilwane ezincelisayo.I-genus γ idala izifo zezinyoni.I-CoV isakazwa kakhulu ngokuthintana okuqondile nokuphumayo noma ngama-aerosol namaconsi. Kukhona nobufakazi bokuthi ingadluliselwa ngomzila we-fecal-oral.

I-Severe acute Respiratory Syndrome coronavirus 2 (i-SARS-CoV-2, noma i-2019-nCoV) igciwane le-RNA elimbozekile elingahlukanisiwe. Kuyimbangela yesifo se-coronavirus 2019 (COVID-19), esithathelwanayo kubantu.

I-SARS-CoV-2 inamaprotheni esakhiwo amaningana afaka i-spike (S), imvilophu (E), ulwelwesi (M) kanye ne-nucleocapsid (N). Iphrotheni ye-spike (S) iqukethe isizinda esibopha i-receptor (i-RBD), enesibopho sokubona isamukeli sendawo yeseli, i-angiotensin eguqula i-enzyme-2 (ACE2). Kutholwe ukuthi i-RBD yephrotheni ye-SARS-CoV-2 S isebenzisana ngokuqinile nesamukeli somuntu se-ACE2 okuholela ku-endocytosis kumaseli aphethe wamaphaphu ajulile kanye nokuphindaphindeka kwegciwane.

Ukutheleleka nge-SARS-CoV-2 kuqala impendulo yokuzivikela komzimba, okubandakanya ukukhiqizwa kwamasosha omzimba egazini. Amasosha omzimba afihliwe ahlinzeka ngokuvikeleka ezifweni zesikhathi esizayo ezivela kumagciwane, ngoba ahlala ohlelweni lokujikeleza kwegazi izinyanga kuya eminyakeni ngemva kokutheleleka futhi azobopha ngokushesha futhi ngokuqinile ku-pathogen ukuze avimbele ukungena kwamangqamuzana nokuphindaphinda. Lawa ma-antibodies abizwa ngokuthi amasosha omzimba anciphisa amandla.

【 UKUQOQWA KANYE NOKULUNGISWA KOMSEBENZI 】

1.Ikhasethi Lokuhlola I-antibody ye-SARS-CoV-2 Neutralizing ihloselwe ukusetshenziswa negazi lomuntu lonke, i-serum noma i-plasma specimens kuphela.

2.Izifanekiso ezicacile kuphela, ezingenayo i-hemolyzed ezinconyiwe ukuze zisetshenziswe nalokhu kuhlolwa. I-Serum noma i-plasma kufanele ihlukaniswe ngokushesha ngangokunokwenzeka ukuze kugwenywe i-hemolysis.

3.Yenza ukuhlola ngokushesha ngemva kokuqoqwa kwesifanekiso. Ungashiyi ama-specimens ekamelweni lokushisa isikhathi eside. Izifanekiso ze-Serum ne-plasma zingagcinwa ku-2-8°C kuze kube yizinsuku ezi-3. Ukugcina isikhathi eside, amasampula e-serum noma e-plasma kufanele agcinwe ngaphansi kuka-20°C. Lonke igazi eliqoqwe yi-venipuncture kufanele ligcinwe ku-2-8°C uma ukuhlolwa kuzokwenziwa phakathi nezinsuku ezi-2 ngemva kokuqoqwa.Ungalifrizi lonke igazi. izibonelo. Igazi eliphelele eliqoqwe ngothi lweminwe kufanele lihlolwe ngokushesha.

4.Iziqukathi eziqukethe ama-anticoagulant njenge-EDTA,citrate,noma i-heparin kufanele zisetshenziselwe ukugcinwa kwegazi lonke.Letha izibonelo ekamelweni lokushisa ngaphambi kokuhlolwa.

5.Izifanekiso eziqandisiwe kufanele zincibilikiswe ngokuphelele futhi zixutshwe kahle ngaphambi kokuhlolwa. Gwema ukuqhwaza okuphindaphindiwe

kanye nokuncibilika kwezibonelo.

6.Uma izifanekiso zizothunyelwa, zipakishe ngokuhambisana nayo yonke imithethonqubo esebenzayo yokuthutha.

ama-etiological agents.

7.I-Icteric, i-lipemic, i-hemolyzed, i-heat treatment kanye ne-sera engcolile ingase ibangele imiphumela eyiphutha.

8. Uma uqoqa igazi lenduku yomunwe nge-lancet kanye nephedi yotshwala, Sicela ulahle iconsi lokuqala

igazi lonke.

1. Letha isikhwama ekamelweni lokushisa ngaphambi kokusivula.Khipha idivayisi yokuhlola esikhwameni esivaliwe futhi usisebenzise ngokushesha ngangokunokwenzeka.

2. Beka idivayisi yokuhlola endaweni ehlanzekile neleveli.

OkweSerum noma Izibonelo zePlasma: Usebenzisa i-Micropipette, bese udlulisela i-5ul serum/plasma esibonelweni somthombo wensiza yokuhlola, bese wengeza okokwehla okungu-2 kwebhafa, bese uqala isibali sikhathi.

Ngegazi Eliphelele (I-Venipuncture/Fingerstick) Izifanekiso: Ncoba umunwe wakho bese ucindezela ngobumnene umunwe wakho, sebenzisa ipayipi lepulasitiki elilahlwayo ukuncela u-10ul wegazi eliphelele emgqeni we-10ul wepayipi lepulasitiki elilahlwayo, bese ulidlulisela emgodini wesifanekiso sedivaysi yokuhlola (uma ivolumu yonke yegazi idlula uphawu, Sicela ukhulule igazi eligcwele ngokweqile ku-pipette), bese wengeza amaconsi angu-2 ebhafa, bese uqala isibali sikhathi. Qaphela: Izifanekiso zingasetshenziswa kusetshenziswa i-micropipette.

3. Linda kuze kuvele ulayini onemibala.Funda imiphumela ngemizuzu eyi-15.Ungatoliki umphumela ngemuva kwemizuzu engama-20.