I-SARS-Cov-2 NOTRALIC
【Ukusetshenziswa okuhlosiwe】
I-SARS-Cov-2 yokungathathi hlangothi Kit I-Antilody Dection Kit iyinhlangano yokuncintisana ye-enzyme-intect exhumene nokutholwa okuphelele nokunakwa kokuthola okuphelele kwama-antibodies ajwayelekile kwa-SARS-CoV-2 e-Sount Serum ne-Plasma. I-SARS- I-Cov-2 yokungathathi hlangothi kwe-antilodte dection Kit ingasetshenziswa njengosizo ekuboneni abantu ngempendulo yokuzivikela komzimba ku-SARS- cov-2, okukhombisa ukutheleleka kwakamuva noma kwangaphambilini. Ama-SARS-COV-2 angathathi hlangothi ikhithi yokutholwa kwe-antibomvu
【Ukuqalisa】
Ukutheleleka kwamaCoronavirus kuvame ukungathathi hlangothi izimpendulo ze-antibody. Amanani e-serocontversion ezigulini ezi-19 angu-50% no-100% ngosuku lwe-7 no-14 lokuthumela uphawu lokuthumela, ngokulandelana. Ukwethula ulwazi, igciwane elihambisanayo elingathathi hlangothi i-antibody egazini kuqashelwa njengelitshe lokunquma ukusebenza kwe-antibody nokuhlushwa okuphezulu kwe-antibody ephakeme. I-Plaque Redruction Ukuvikelwa kokungathathi hlangothi (i-PRNT) bekuqashelwa njengezinga legolide lokuthola ama-antibodies angathathi hlangothi. Kodwa-ke, ngenxa yokuphamba kwayo okuphansi kanye nesidingo esiphakeme sokusebenza, i-PRNT ayisebenzi ngesilinganiso esikhulu se-serodiagnosis kanye nokuhlolwa kokugomela. I-SARS-COV-2 yokuthola i-antilody dection Kit isuselwa kwindlela yokuncintisana ye-enzyme-lixhumanisa ne-Assay (ELISA), engabona i-antiwody engathathi hlangothi ekhasini legazi futhi lifinyelela ngokukhethekile amazinga okuhlushwa alolu hlobo lwe-antibody.
【Inqubo yokuhlola】
1.I-adrodwa runes, i-aliquot 120μL yesisombululo esilungiselelwe i-Hace2-HRP esilungiselelwe.
I-2.ADD 6 μL yamaCalibrators, amasampula angaziwa, izilawuli zekhwalithi kushubhu ngalinye bese uxuba kahle.
I-3.Transfer 100μL yengxube ngayinye elungiselelwe esiteji 2 ibe yimithombo emincane ehambisanayo ngokusho ukucushwa kokuhlolwa okudelwe.
3.Copha ipuleti nge-Plate Sealer futhi ifaka ku-37 ° C imizuzu engama-60.
I-4.Remove the Plate Sealer bese ugeza ipuleti nge-300 μl yesixazululo esingu-1 × ngageza ngakolunye uhlangothi amahlandla amane.
5.Thola ipuleti ethawula lephepha ukuze ususe uketshezi oluyinsalela emithonjeni ngemuva kokugeza izinyathelo.
I-6.Add 100 μL yesisombululo se-TMB kuyo ngasinye futhi ifaka ipuleti ebumnyameni ngo-20 - 25 ° C imizuzu engama-20.
7.ADD 50 μL wesisombululo sokuma komunye umthombo ngamunye ukumisa ukusabela.
8. Fundenda umuzwa ku-Microplate Reader ku-450 nm kungakapheli imizuzu eyi-10 (630nn njengoba kunconyelwa ukufakwa okuphezulu kokusebenza okuphezulu.