I-SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)

【UKUSETSHENZISWA OKUHLOSIWE】

I-SARS-CoV-2 Neutralizing Antibody Detection Kit iyi-Competitive Enzyme-Linked Immunosorbent Assay (ELISA) ehloselwe ukutholwa kwekhwalithi nengxenye yobuningi bamasosha omzimba angathathi hlangothi ku-SARS-CoV-2 ku-serum yomuntu kanye ne-plasma. I-SARS-CoV-2 Neutralizing Antibody Detection Kit ingasetshenziswa njengosizo ekuhlonzeni abantu abanempendulo yokuzivikela eguquguqukayo ku-SARS-CoV-2, okubonisa ukutheleleka kwakamuva noma kwangaphambilini. I-SARS-CoV-2 Neutralizing Antibody Detection Kit akufanele isetshenziselwe ukuxilonga ukutheleleka nge-SARS-CoV-2 okuyingozi.

【ISINGENISO】

Ukutheleleka kweCoronavirus kuvame ukubangela ukungezwani kwama-antibody. Amazinga e-seroconversion ezigulini ze-COVID-19 angama-50% kanye ne-100% ngosuku lwe-7 no-14 lwezimpawu zokuqala, ngokulandelana. Ukwethula ulwazi, igciwane elihambisanayo elinciphisa i-antibody egazini libonwa njengento eqondiwe yokunquma ukusebenza kwe-antibody kanye nokugxila okuphezulu kwe-antibody engathathi hlangothi kubonisa ukusebenza kahle kokuvikela okuphezulu. I-Plaque Reduction Neutralization Test (PRNT) iqashelwa njengezinga legolide lokuthola amasosha omzimba angathathi hlangothi. Kodwa-ke, ngenxa yomthamo wayo ophansi kanye nemfuneko ephezulu yokusebenza, i-PRNT ayisebenzi ekuxilongweni okukhulu kwe-serodiagnosis kanye nokuhlolwa komgomo. I-SARS-CoV-2 Neutralizing Antibody Detection Kit isuselwe kunqubo yeCompetitive Enzyme-Linked Immunosorbent Assay (ELISA), ekwazi ukubona i-antibody engathathi hlangothi kusampula yegazi futhi ifinyelele ngokukhethekile emazingeni okuhlushwa alolu hlobo lwe-antibody.

 【Inqubo Yokuhlola】

1.Kumashubhu ahlukene, i-aliquot 120μL Yesixazululo se-hACE2-HRP esilungisiwe.

2.Engeza u-6 μL wezikali, amasampuli angaziwa, izilawuli zekhwalithi kushubhu ngayinye bese uxuba kahle.

3.Dlulisa u-100μL wengxube ngayinye elungiselelwe esinyathelweni sesi-2 ungene emithonjeni ye-microplate ehambisanayo ngokuya ngokucushwa kokuhlolwa okuklanywe kusengaphambili.

3. Mboza ipuleti nge-Plate Sealer bese ufukamela ku-37°C imizuzu engu-60.

4.Khipha i-Plate Sealer bese ugeza ipuleti cishe ngo-300 μL we-1× Geza Isixazululo somthombo izikhathi ezine.

5.Thepha ipuleti ithawula lephepha ukuze ukhiphe uketshezi olusele emithonjeni ngemva kokuwasha izinyathelo.

6.Faka i-100 μL ye-TMB Solution emthonjeni ngamunye bese ufukamela ipuleti ebumnyameni ngo-20 - 25°C imizuzu engu-20.

7.Yengeza u-50 μL we-Stop Solution emthonjeni ngamunye ukuze umise ukusabela.

8.Funda ukumunca kumfundi we-microplate ku-450 nm phakathi nemizuzu eyi-10 (630nm njengesisekeli sinconywa ekusebenzeni okunembayo okuphezulu.