I-RSV Respiratory Syncytial Virus Ag Test

Incazelo emfushane:

I-Respiratory Syncytial Virus (RSV)igciwane elithathelwana kakhulu elithinta ngokuyinhloko umgudu wokuphefumula. Ingenye yezimbangela ezivame kakhulu zezifo zokuphefumula, ikakhulukazi ezinsaneni, izingane ezincane kanye nabantu asebekhulile. Ukutheleleka kwe-RSV kusuka ezimpawu ezithambile, ezinjengamakhaza kuya ezifweni ezinzima zokuphefumula njenge-bronchiolitis ne-pneumonia. Igciwane lisakazeka ngamaconsi okuphefumula, ukuthintana ngokuqondile, noma izindawo ezingcolile. I-RSV ivame kakhulu phakathi nezinyanga zasebusika nasekuqaleni kwentwasahlobo, okwenza ukuxilongwa okufika ngesikhathi nokunembayo kubaluleke kakhulu ekulawuleni ngempumelelo nokulawula ukuqubuka kwezifo.

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Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane Yomkhiqizo:

Izinhlobo zokuhlolwa kwe-RSV:
- Ukuhlolwa Okusheshayo kwe-RSV Antigen:
  - Isebenzisa ubuchwepheshe bokugeleza kwe-immunochromatographic lateral ukuze ithole ngokushesha ama-antigen e-RSV kumasampuli okuphefumula (isb., ukuswayipha emakhaleni, ukuswayipha komphimbo).
  - Inikeza imiphumela ku15-20 imizuzu.
- I-RSV Molecular Test (PCR):
  - Ithola i-RSV RNA isebenzisa amasu emolekyuli azwela kakhulu afana nereverse transcription-polymerase chain reaction (RT-PCR).
  - Idinga ukucubungula ilabhorethri kodwa izipesheliukuzwela okuphezulu nokucacisa.
- I-RSV Viral Culture:
  - Kubandakanya ukukhulisa i-RSV endaweni elawulwa ngelebhu.
  - Akuvamile ukusetshenziswa ngenxa yesikhathi eside sokuguqula.
Izinhlobo zamasampuli:
- I-Nasopharyngeal swab
- I-swab yomphimbo
- I-aspirate yamakhala
- I-bronchoalveolar lavage (ezimweni ezinzima)
Inani labantu eliqondiwe:
- Izinsana nezingane ezincane ezinezimpawu ezinzima zokuphefumula.
- Iziguli esezikhulile ezinenkinga yokuphefumula.
- Abantu abane-immunocompromised abanezimpawu ezinjengomkhuhlane.
Ukusetshenziswa Okuvamile:
- Ukwehlukanisa i-RSV kwezinye izifo zokuphefumula ezifana nomkhuhlane, i-COVID-19, noma i-adenovirus.
- Ukugqugquzela izinqumo zokwelashwa ezifika ngesikhathi futhi ezifanele.
- Ukuqapha impilo yomphakathi ngesikhathi sokuqubuka kwe-RSV.

Isimiso:

Ukuhlola kusebenzisaukuhlolwa kwe-immunochromatographic (ukugeleza kwe-lateral)ubuchwepheshe bokuthola ama-antigen e-RSV.
Ama-antigen e-RSV kusampula yokuphefumula yesiguli abopha amasosha omzimba athile ahlanganiswe negolide noma izinhlayiya ezinemibala emgqeni wokuhlola.
Ulayini obonakalayo uyakha endaweni yokuhlola (T) uma ama-antigen e-RSV ekhona.

Ukwakhiwa:

Ukwakheka	Inani	Ukucaciswa
IFU	1	/
Hlola ikhasethi	25	/
I-diluent yokukhishwa	500μL1 Ishubhu 25	/
Ithiphu ye-Dropper	/	/
I-Swab	1	/

Inqubo Yokuhlola:


1. Geza izandla zakho	2. Hlola okuqukethwe kwekhithi ngaphambi kokuhlolwa, hlanganisa nokufakwa kwephakheji, i-test cassette, i-buffer, i-swab.	3.Beka ishubhu yokukhipha endaweni yokusebenza.	4.Hlubula isivalo se-aluminium foil kusukela phezulu kweshubhu yokukhipha equkethe isigcinalwazi sokukhipha.

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5.Khipha ngokucophelela i-swab ngaphandle kokuthinta ichopho.Faka yonke ichopho le-swab kusuka ku-2 kuya ku-3 cm ekhaleni elingakwesokudla.Qaphela ukuphuka kwe-swab yamakhala.Ungakuzwa lokhu ngeminwe yakho lapho ufaka i-swab yamakhala noma hlola. yona emncane. Gcoba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana engu-15,Manje thatha i-swab yamakhala efanayo futhi uyifake kwenye ikhala.Gwaba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana eyi-15. Sicela wenze ukuhlolwa ngokuqondile nesampula futhi ungakwenzi
ayishiye imile.

6.Beka i-swab eshubhuni yokukhipha. Zungezisa i-swab cishe imizuzwana eyi-10, Zungezisa i-swab eshubhuni lokukhipha, ucindezela ikhanda le-swab phakathi kweshubhu ngenkathi ucindezela izinhlangothi zeshubhu ukuze ukhiphe uketshezi oluningi. ngangokunokwenzeka kusuka ku-swab.


7. Khipha i-swab ephaketheni ngaphandle kokuthinta i-padding.	8.Xuba kahle ngokuqhweba phansi kweshubhu.Beka amathonsi ama-3 esampula ngokuqondile emthonjeni wesampula wekhasethi lokuhlola.Funda umphumela ngemva kwemizuzu engu-15. Qaphela: Funda umphumela kungakapheli imizuzu engama-20. Ngaphandle kwalokho, kunconyelwa ukucela isicelo sokuhlolwa.

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