Umkhiqizi we-OEM wase-China Medomics Infectious COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist

Manje sinabasebenzisi abaningi abahle kakhulu bezisebenzi ezinhle kakhulu zokumaketha nge-inthanethi, i-QC, futhi sisebenza nezinhlobo zenkinga enzima ngaphakathi kwendlela yokukhiqiza yoMkhiqizi we-OEM China Medomics Infectious COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist, Besiyingxenye eyodwa. kubakhiqizi bakho abakhulu abangu-100% eShayina.Inqwaba yamabhizinisi amakhulu okuhweba angenisa imikhiqizo nezisombululo ezivela kithi, ukuze sikwazi ukukunikeza kalula ithegi yamanani enenzuzo kakhulu enekhwalithi efanayo kunoma ubani onentshisekelo kithi.
Manje sinabasebenzisi abaningi abasebenza kahle kakhulu be-inthanethi ukumaketha, i-QC, futhi sisebenza nezinhlobo zenkinga enzima ngaphakathi kwendlela yokukhiqizaImboni ye-Antibody Neutralizing, Umkhiqizi we-Antibody Neutralizing, Umhlinzeki we-Antibody Neutralization, Ukuhlolwa kwe-Antibody Neutralizing, I-China Medical, I-antibody engathathi hlangothi, Kungakhathaliseki ukuthi ukhetha umkhiqizo wamanje kukhathalogi yethu noma ufuna usizo lobunjiniyela lohlelo lwakho lokusebenza, ungakhuluma nesikhungo sethu senkonzo yamakhasimende mayelana nezidingo zakho zokuthola usizo.Singakunikeza ikhwalithi enhle ngentengo yokuncintisana ngawe mathupha.

【ISIMISO】

I-SARS-CoV-2I-antibody engathathi hlangothiI-Detection Kit isuselwe endleleni yokuncintisana ye-ELISA.

Kusetshenziswa isizinda esibophezelayo se-purified receptor (RBD), amaprotheni asuka ku-viral spike (S) protein kanye ne-host cell

i-receptor ACE2, lokhu kuhlola kuklanyelwe ukulingisa ukusebenzisana kwe-virus-host neutralizing.

Ama-Calibrator, Izilawuli Zekhwalithi, kanye namasampula e-serum noma e-plasma axutshwe ngawodwa kahle ekuhlanjululweni

ibhafa equkethe i-hACE2-HRP conjugate ecashunwe kumashubhu amancane.Khona-ke izingxube zidluliselwa phakathi

Imithombo ye-microplate equkethe i-immobilized recombinant SARS-CoV-2 RBD fragment (RBD)

ukufukamela.Ngesikhathi sokufukamela imizuzu engama-30, i-antibody ethize ye-RBD kuma-calibrator, i-QC kanye

amasampula azoqhudelana ne-hACE2-HRP ngokubopha ngokuqondile i-RBD enganyakazi emithonjeni.Ngemva

ekufukameleni, imithombo igezwa izikhathi ezi-4 ukuze kukhishwe i-hACE2-HRP conjugate engaboshiwe.Isixazululo se

I-TMB ibe seyengezwa futhi ifukamele imizuzu engama-20 ekamelweni lokushisa, okuholela ekwakhekeni kwe-a

umbala oluhlaza okwesibhakabhaka.Ukuthuthukiswa kombala kumisiwe ngokungezwa kwe-1N HCl, futhi ukumunca kuyinto

kukalwe i-spectrophotometrically ku-450 nm.Ukuqina kombala owenziwe kuhambisana ne

inani le-enzyme elikhona, futhi lihlobene ngokuphambene nenani lamazinga ahlolwe ngendlela efanayo.

Ngokuqhathanisa nejika lokulinganisa elakhiwe ama-calibrator anikeziwe, ukugxila kwe

amasosha omzimba anciphisa isampula engaziwa abe esebalwa.

【IZINTO EZIDINGEKAYO KODWA AKUNIKEZWE】

1. Amanzi acwengekile noma ahlanjululwe

2. Amapayipi anembile: 10μL, 100μL, 200μL no-1 mL

3. Amathiphu e-pipette alahlwayo

4. Umfundi we-Microplate okwazi ukufunda ukumunca ku-450nm.

5. Iphepha le-Absorbent

6. Iphepha legrafu

7. I-Vortex mixer noma okulinganayo

【UKUQOQWA KANYE NESIGCINISO】

1. Amasampula eSerum nePlasma aqoqwe kumashubhu aqukethe i-K2-EDTA angasetshenziswa kule kit.

2. Izibonelo kufanele zifakwe i-cap futhi zingagcinwa amahora angama-48 ku-2 °C - 8 °C ngaphambi kokuhlolwa.

Izibonelo ezigcinwe isikhathi eside (kuze kufike ezinyangeni eziyisi-6) kufanele ziqandiswe kanye kuphela ku -20 °C ngaphambi kokuhlolwa.

Gwema imijikelezo yokuqhwaza ephindaphindiwe.

I-PROTOCOL

【Ukulungiswa kwe-Reagent】

1. Wonke ama-reagents kufanele akhishwe esiqandisini futhi avunyelwe ukuthi abuyele ekamelweni lokushisa ngaphambi kokusetshenziswa

(20° kuya ku-25°C).Gcina wonke ama-reagents esiqandisini ngokushesha ngemva kokusetshenziswa.

2. Wonke amasampuli nezilawuli kufanele zivuthwe ngaphambi kokusetshenziswa.

3. I-hACE2-HRP Ukulungiswa Kwesixazululo: Nciphisa i-hACE2-HRP ukugxilisa ingqondo ku-1: 51 dilution ratio ne-Dilution

Ibhafa.Isibonelo, hlambulula u-100 μL we-hACE2-HRP ugxile ngo-5.0mL we-HRP Dilution Buffer ukuze

yenza isisombululo se-hACE2-HRP.

4. 1× Ukulungiselela Isixazululo Sokugeza: Nciphisa Isixazululo se-20× Wash ngamanzi akhiqiziwe noma acwecwe nge-

isilinganiso sevolumu 1:19.Isibonelo, hlambulula u-20 mL we-20× Wash Solution ngo-380 mL we-deionized noma

amanzi distilled ukwenza 400 mL of 1× Geza Isixazululo.

【Inqubo Yokuhlola】

1. Kumashubhu ahlukene, i-aliquot 120μL Yesixazululo se-hACE2-HRP esilungisiwe.

2. Engeza u-6 μL wezikali, amasampula angaziwa, izilawuli zekhwalithi kushubhu ngalinye futhi uhlanganise kahle.

3. Dlulisa u-100μL wengxube ngayinye elungiselelwe esinyathelweni sesi-2 emithonjeni ye-microplate ehambisanayo ngokuya

ekucushweni kokuhlola okuklanywe ngaphambili.

3. Mboza ipuleti nge-Plate Sealer bese ufukamela ku-37°C imizuzu engu-30.

4. Khipha I-Plate Sealer bese ugeza ipuleti cishe ngo-300 μL we-1× Geza Isixazululo somthombo izikhathi ezine.

5. Thinta ipuleti ithawula lephepha ukuze ukhiphe uketshezi olusele emithonjeni ngemva kokugeza izinyathelo.

6. Faka i-100 μL ye-TMB Solution emthonjeni ngamunye bese ufukamela ipuleti ebumnyameni ngo-20 - 25°C imizuzu engu-20.

7. Engeza u-50 μL we-Stop Solution emthonjeni ngamunye ukuze umise ukusabela.

8. Funda ukumuncwa kumfundi we-microplate ku-450 nm phakathi nemizuzu eyi-10 (630nm njengesesekeli

kunconyelwe ukusebenza ngokunemba okuphezulu).