Ikhithi Yokubona ye-SARS-CoV-2 yesikhathi sangempela ye-RT-PCR

Le kit ihloselwe ukutholwa kwekhwalithi ye-in vitro yezakhi zofuzo ze-ORF1ab ne-N ezivela ku-2019-nCoV ku-pharyngeal swab noma izibonelo ze-bronchoalveolar lavage eziqoqwe kumacala asolwayo e-Coronavirus 2019 (COVID-19), abasolwa ngamaqoqo amacala, noma abanye abantu abadinga u-2019. - Ukuxilongwa kokutheleleka nge-nCoV noma ukuxilongwa kokuhlukaniswa.

 Isithombe 002

Ikhithi yakhelwe ukutholwa kwe-RNA ka-2019-nCoV kuma-specimens kusetshenziswa ubuchwepheshe be-multiplex besikhathi sangempela se-RTPCR kanye nezifunda ezilondoloziwe ze-ORF1ab kanye nezakhi zofuzo ze-N njengamasayithi okuqondiwe kuwo ama-primers nama-probe.Ngesikhathi esifanayo, le khithi iqukethe isistimu yokuthola ukulawula okungapheli (Ufuzo lokulawula lubhalwe i-Cy5) ukuze kuqashwe inqubo yokuqoqwa kwesampula, ukukhishwa kwe-nucleic acid ne-PCR nokunciphisa imiphumela engemihle engamanga.

 Isithombe 004

Izici Eziyinhloko:

1. Ukukhulisa okusheshayo, okunokwethenjelwa kanye nokubandakanywa kokutholwa : I-SARS njenge-coronavirus nokutholwa okuqondile kwe-SARS-CoV-2

2. Isinyathelo esisodwa se-RT-PCR reagent (impushana e-lyophilized)

3. Kubandakanya ukulawula okuhle nokubi

4. Ukuhamba ngezinga lokushisa elivamile

5. Ikhithi ingahlala izinzile kuze kufike ezinyangeni eziyi-18 igcinwe ku -20℃.

6. CE igunyaziwe

Ukugeleza :

1. Lungiselela i-RNA ekhishiwe kwa-SARS-CoV-2

2. Nciphisa ukulawula okuhle kwe-RNA ngamanzi

3. Lungiselela i-PCR master mix

4. Faka i-PCR master mix ne-RNA epuleti noma ishubhu ye-PCR yesikhathi sangempela

5. Qalisa ithuluzi le-PCR lesikhathi sangempela

 Isithombe 006


Isikhathi sokuthumela: Nov-09-2020

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