I-SARS-Cov-2 Isikhathi sangempela se-RT-PRCR

Le khithi yenzelwe ukutholwa kwe-vitro okufanelekile kwe-Orf1ab kanye ne-n izinhlobo zofuzo ezivela ku-2019-NCOV e-Phatyngengeal Swab noma ama-bronchoalveolar lavage amacala aqoqwe kusuka ku-coronavirus isifo, amaqoqo asolwa ngamacala, noma abanye abantu abadinga unyaka we-2019 - Ukuxilongwa ngokutheleleka ngegciwane le-NCOV noma ukunqunywa kokwehlukanisa.

I-KIT yenzelwe ukutholwa kwe-RNA ka-2019-NCOV ku-speciments isebenzisa ubuchwepheshe be-Multiplex Cuere Bukhomba nobuchwepheshe be-Orf1ab kanye nezindawo zokuhlobisa amasayithi ahlosiwe. Ngakolunye uhlangothi, le kit iqukethe uhlelo lokutholwa kokulawulwa okuphelele (uhlobo lokulawula lubhalwe nguCy5) ukuqapha inqubo yokuqoqwa kwama-specimen, ukukhishwa kwe-nucleic acid kanye ne-PCR futhi kunciphise imiphumela emibi yamanga.

Izici ezibalulekile:

1. I-Rapid, I-Amplification ethembekile kanye nokutholwa kokutholwa: AbakwaSars njengeCoronavirus nokutholwa okuthile kwe-SARS-CoV-2

2. Isinyathelo esisodwa se-RT-PCR Reagent (i-Lyophilezed Powder)

3. Kubandakanya izilawuli ezinhle nezingalungile

4. Ukuhamba ekushiseni okujwayelekile

I-5. Ikhithi ingagcina izinzile kuze kufike ezinyangeni eziyi-18 ezigcinwe ku-20 ℃.

6. I-CE yavunywa

Ukuhamba:

1. Lungiselela i-RNA ekhishwe ku-SARS-CoV-2

2. Dlulisa i-RNA enhle ye-RNA enamanzi

3. Lungiselela ukuxubana kwe-PCR MAST

4. Faka isicelo se-PCR MAster Mix ne-RNA kupuleti ye-PCR yesikhathi sangempela noma ishubhu

5. Sebenzisa ithuluzi le-PCR yesikhathi sangempela