I-Immunology yisifundo esiyinkimbinkimbi esiqukethe ulwazi oluningi lochwepheshe.Lesi sihloko sihlose ukukwazisa ukuthi imikhiqizo yethu isebenzisa ulimi olufushane oluqondakalayo.
Emkhakheni wokutholwa ngokushesha, ukusetshenziswa kwasekhaya kuvame ukusebenzisa indlela yegolide ye-colloidal.
Ama-nanoparticles egolide ahlanganiswa kalula namasosha omzimba, ama-peptides, ama-oligonucleotides okwenziwa, namanye amaprotheni ngenxa yokuhlangana kwamaqembu e-sulfhydryl (-SH) endaweni yegolide.3-5.Ama-conjugates e-Gold-biomolecule afakwe kabanzi ezinhlelweni zokuxilonga, lapho umbala wawo obomvu ogqamile usetshenziswa ekuhlolweni kwasekhaya kanye nendawo yokunakekela njengokuhlolwa kokukhulelwa kwasekhaya.
Ngoba ukusebenza kulula, umphumela kulula ukuyiqonda, elula, esheshayo, enembile nezinye izizathu.Indlela yegolide ye-Colloidal iyindlela eyinhloko yokuthola ngokushesha emakethe.
Izivivinyo ezincintisanayo namasemishi zingamamodeli angu-2 ayinhloko endleleni yegolide ye-colloidal, Zidonse isithakazelo ngenxa yefomethi yazo enobungane yabasebenzisi, izikhathi ezimfushane zokuhlola, iziphazamiso ezincane, izindleko eziphansi, nokuba lula ngokusetshenziswa izisebenzi ezingakhethekile.Le nqubo isekelwe ekusebenzisaneni kwamakhemikhali e-antigen-antibody hybridization.Imikhiqizo yethu yakhiwe izingxenye ezine: iphedi yesampula, okuyindawo okulahlwa kuyo isampula;i-conjugate pad, lapho amathegi afakwe ilebula ahlanganiswe nezinto zokuqashelwa kwe-biorecognition;i-membrane yokusabela equkethe umugqa wokuhlola kanye nomugqa wokulawula wokusebenzisana kwe-antigen-antibody;kanye nephedi elimuncayo, eligcina imfucuza.
1.Isimiso Sokuhlola
Amasosha omzimba amabili abopha ama-epitopes ahlukene akhona ku-molecule yegciwane asetshenziswa.Eyodwa (i-antibody enamathelayo) ebhalwe nge-nanoparticles yegolide eyi-colloidal kanti enye (thwebula i-antibody) igxiliswe endaweni ye-NC membrane.I-antibody embozayo isesimweni sokuphelelwa amanzi emzimbeni ngaphakathi kwephedi ye-conjugate.Lapho isixazululo esijwayelekile noma isampula yengezwe kuphedi yesampula yomugqa wokuhlola, isibophezelo singancibilika ngokushesha lapho sithinta indawo enamanzi equkethe igciwane.Khona-ke i-antibody yakha inkimbinkimbi enegciwane esigabeni soketshezi futhi yaqhubekela phambili ngokuqhubekayo yaze yathathwa yi-antibody ebekwe endaweni engaphezulu ye-NC membrane, eyakhiqiza isignali ngesilinganiso mayelana nokugxilwa kwegciwane.Ngaphezu kwalokho, i-antibody eyengeziwe eqondene ne-antibody embozayo ingasetshenziswa ukukhiqiza isignali yokulawula.Iphedi emuncayo itholakala phezulu ukuze ihehe i-capillarity eyenza i-immune complex idonselwe ku-antibody engashintshi.Umbala obonakalayo uvele ngaphansi kwemizuzu eyi-10, futhi ukuqina kunquma inani legciwane.Ngamanye amazwi, igciwane eliningi elalikhona kusampula, kulapho kubonakala khona ibhande elibomvu.
Ake ngichaze kafushane ukuthi lezi zindlela ezimbili zisebenza kanjani:
1.Indlela ye-anti sandwich ephindwe kabili
Isimiso sendlela ye-anti sandwich ephindwe kabili, esetshenziselwa kakhulu ukuthola iphrotheni enkulu yesisindo se-molecular (anti). Kudingeka ama-anti amabili ukuze kukhonjwe amasayithi ahlukene e-antigen.
2. Indlela yokuncintisana
Indlela yokuncintisana isho indlela yokuthola i-antigen embozwe ulayini wokutholwa kanye ne-antibody yophawu lwegolide lwe-antigen okufanele luhlolwe.Imiphumela yale ndlela ifundwa ngokuphambene nemiphumela yendlela yesangweji, nenye. umugqa kuphozithivu kanye nemigqa emibili kwenegethivu.
Isikhathi sokuthumela: Dec-03-2019