I-Immunology iyindaba eyinkimbinkimbi equkethe ulwazi oluningi lobuchwepheshe. Lo mbhalo uhlose ukukwazisa emikhiqizweni yethu usebenzisa ulimi olufushane olufushane kakhulu.
Emkhakheni wokutholwa okusheshayo, ukusetshenziswa kwasekhaya kuvame ukusebenzisa indlela yegolide ye-colloidal.
Ama-nanoparticles egolide ahlanganiswe kalula ama-antibodies, ama-antigodes, ama-oligonucleotides okwenziwa, namanye amaprotheni ngenxa yokuhlangana kwamaqembu e-sulfhydryl (-SH) egolide3-5. IGold-Biomolecule Connjugates yafakwa kabanzi kwizicelo zokuxilonga, lapho kusetshenziswa umbala wabo obomvu ekhaya kanye nokuhlolwa kokunakekelwa okufana nokuhlolwa kokukhulelwa kwasekhaya
Ngoba ukusebenza kulula, umphumela kulula ukusiqonda, elula, esheshayo, esheshayo nezinye izizathu. I-Colloidal Gold Way iyindlela engcono yokutholwa ngokushesha emakethe.
AMASO AMAZWI WOKUXHUMANA NOKUXHUMANISA amamodeli ama-2 aphambili e-Colloidal Gold Way, adonsele inzalo ngenxa yamafomethi abo anobungani, izikhathi ezimfishane zezilwane, ukungqubuzana okuphansi, kanye nokuba lula ngokusetshenziswa ngabasebenzi abangakhethekile. Le ndlela isuselwa ekuxhumaneni kwe-biochemical kwe-antigen-antibody hybridization. Imikhiqizo yethu yakhiwa izingxenye ezine: i-pad yesampula, okuyindawo lapho isampula ilahlwa khona; I-Conjugate Pad, lapho amathegi abhalwe amabhayisekili ahlanganiswa khona ngentengo yokubukwa; UMembrane wokuphendula aqukethe umugqa wokuhlola kanye nomugqa wokulawula ukusebenzelana kwe-antigen-antibody; futhi i-amunbent pad, egcina imfucuza.
Isimiso se-1.Assay
Kusetshenziswa ama-antibopho amabili ama-antibopho ahlukile ku-virus molecule asetshenziswa. I-One (i-antibody) efakwe ilebula nge-nanoparticles yegolide ye-colloidal kanye nezinye (ukuthwebula i-antibody) kugxilwe ezindaweni ze-NC membrane. I-antibod ye-coating isesimweni esinamanzi ngaphakathi kwe-conjugate pad. Lapho isixazululo esijwayelekile noma isampula sengezwe ephepheni lesampula lomucu wokuhlola, i-binder ingachithwa ngaso leso sikhathi ngegciwane eliphakathi nendawo enamanzi. Lapho-ke i-antibody yakha isakhiwo sale gciwane esigabeni se-liquid futhi yaqhutshwa phambili ngokuqhubekayo ize ithunjwe yi-antibod elungiselelwe endaweni ye-NC. Ngaphezu kwalokho, i-antibody eyengeziwe eqondile kwi-antibody ye-coating ingasetshenziswa ukukhiqiza isiginali yokulawula. I-Abserbent Pad itholakala phezulu ukuze ukhiphe i-capillarity eyenza ukuthi i-immun dex ikwazi ukudonswa ku-antilody engaguquki. Umbala obonakalayo uvele ngaphansi kwemizuzu eyi-10, futhi ubukhulu bunquma inani legciwane. Ngakolunye igama, leli gciwane elalikhona kusampula, kwavela ukuthi babukeke kade band.
Ake ngichaze kafushane ukuthi lezi zindlela ezimbili zisebenza kanjani:
1.I-1.Deble anti sandwich indlela
Indlela ephindwe kabili ye-sandwich ye-sandwich, esetshenziselwa ikakhulukazi ukutholwa kwamaprotheni amakhulu wesisindo se-molecular (anti) .two anti kudingeka ukuqondisa amasayithi ahlukene we-antigen.
2. Indlela yokuncintisana
Indlela yokuncintisana isho indlela yokutholwa ye-Antigen ehlanganiswe umugqa wokutholwa kanye ne-antibody yophawu lwegolide lwe-antigen ukuze ihlolwe. umugqa emigqeni emihle neyembili kwe-negative.
Isikhathi se-Pop: Dec-03-2019