I-Monkeypox Virus (MPV) Nucleic Acid Detection Kit
ISINGENISO
Ikhithi isetshenziselwa ukuthola ikhwalithi ye-in vitro yezigameko ezisolwayo ze-Monkeypox Virus (MPV), amacala ahlanganisiwe kanye nezinye izimo ezidinga ukuhlolelwa ukutheleleka nge-Monkeypox Virus.
Ikhithi isetshenziselwa ukuhlonza isakhi sofuzo se-f3L se-MPV kuma-swabs omphimbo namasampula e-nasal swab.
Imiphumela yokuhlolwa yale khithi ingeyenkomba yomtholampilo kuphela futhi akufanele isetshenziswe njengeyona ndlela yodwa yokuxilongwa komtholampilo. Kunconywa ukwenza ukuhlaziywa okuphelele kwesimo ngokusekelwe emtholampilo wesiguli
ukubonakaliswa nokunye ukuhlolwa kwelabhorethri.
Ukusetshenziswa Okuhlosiwe
| Uhlobo lokuhlola | umphimbo kanye ne-swab yamakhala |
| Uhlobo lokuhlola | Ikhwalithi |
| Izinto zokuhlola | I-PCR |
| Usayizi wephakethe | 48 izivivinyo/1 ibhokisi |
| Izinga lokushisa lesitoreji | 2-30 ℃ |
| Impilo yeshelufu | Izinyanga ezingu-10 |
Isici somkhiqizo
Isimiso
Le khithi ithatha ukulandelana okuqondile okulondoloziwe kofuzo lwe-MPV f3L njengendawo eqondiwe. Ubuchwepheshe besikhathi sangempela be-fluorescence quantitative PCR kanye nobuchwepheshe bokukhululwa okusheshayo be-nucleic acid busetshenziselwa ukuqapha i-viral nucleic acid ngokushintsha isignali ye-fluorescence yemikhiqizo yokukhulisa umsindo. Isistimu yokuthola ihlanganisa ukulawula kwekhwalithi yangaphakathi, esetshenziselwa ukuqapha ukuthi ingabe akhona yini ama-PCR inhibitor kumasampuli noma ukuthi amaseli kumasampuli athathiwe, okungavimbela ngempumelelo isimo esingesihle esingamanga.
IZINTO EZIYINHLOKO
Ikhithi iqukethe ama-reagents okucubungula izivivinyo ezingama-48 noma ukulawulwa kwekhwalithi, okuhlanganisa izingxenye ezilandelayo:
I-Reagent A
| Igama | Izingxenye eziyinhloko | Ubuningi |
| Ukutholwa kwe-MPV i-reagent | Ishubhu yokusabela iqukethe i-Mg2+, I-f3L gene / Rnase P primer probe, i-react buffer, i-enzyme ye-Taq DNA. | 48 izivivinyo |
I-ReagentB
| Igama | Izingxenye eziyinhloko | Ubuningi |
| I-MPV Ukulawula Okuhle | Iqukethe ingxenye yethagethi ye-MPV | 1 ithubhu |
| I-MPV Ukulawula Okungalungile | Ngaphandle kwe-MPV target fragment | 1 ithubhu |
| I-reagent yokukhishwa kwe-DNA | I-reagent iqukethe i-Tris, i-EDTA kanye ne-Triton. | 48pcs |
| I-reagent yokubuyisela kabusha | Amanzi aphethwe yi-DEPC | 5ML |
Qaphela: Izingxenye zezinombolo zeqoqo ezahlukene azikwazi ukusetshenziswa ngokushintshana
【Izimo Zesitoreji Kanye Nempilo Yeshelufu】
1.I-Reagent A/B ingagcinwa ku-2-30°C, kanti isikhathi seshelufu siyizinyanga eziyi-10.
2.Sicela uvule ikhava yeshubhu yokuhlola kuphela uma usulungele ukuhlolwa.
3.Ungasebenzisi amashubhu okuhlola ngaphezu kosuku lokuphelelwa yisikhathi.
4.Ungasebenzisi ishubhu lokubona elivuzayo.
【Ithuluzi Elisebenzayo】
Ifanele isistimu yokuhlaziya ye-LC480 PCR, i-Gentier 48E Automatic PCR system analysis, ABI7500 PCR system analysis.
【Izidingo Zesampula】
1.Izinhlobo zesampula ezisebenzayo: amasampula e-throat swabs.
2.Isixazululo sesampula:Ngemva kokuqinisekisa, kunconywa ukusebenzisa usawoti ovamile noma ishubhu lokulondoloza igciwane elikhiqizwe yi-Hangzhou Testsea biology ukuze kuqoqwe isampula.
umqala:sula amathoni epharyngeal amabili kanye nodonga lwangemuva lwepharyngeal ngeswabhu yesampula eyinyumba elahlwayo, cwilisa i-swab eshubhuni eliqukethe isisombululo sesampula esingu-3mL, ulahle umsila, bese uqinisa ikhava yeshubhu.
3.Isampula yokugcina nokulethwa:Amasampula azohlolwa kufanele ahlolwe ngokushesha ngangokunokwenzeka. Izinga lokushisa lokuthutha kufanele ligcinwe ku-2 ~ 8 ℃. Amasampula angahlolwa kungakapheli amahora angu-24 angagcinwa ku-2 ℃ ~ 8 ℃ futhi uma amasampula engakwazi ukuhlolwa kungakapheli amahora angu-24, kufanele agcinwe ngaphansi noma alinganayo. kuya ku -70 ℃ (uma singekho isimo sokugcina sika -70 ℃, singagcinwa ku -20 ℃ okwesikhashana), gwema ukuphindaphinda
ukuqhwaza nokuncibilika.
I-4.Iqoqo lesampula elifanelekile, ukugcinwa, nokuthutha kubalulekile ekusebenzeni kwalo mkhiqizo.
【Indlela Yokuhlola】
1.Ukucubungula isampula kanye nokwengezwa kwesampula
1.1 Ukucubungula isampula
Ngemva kokuxuba isixazululo sesampula esingenhla namasampula, thatha u-30μL wesampula uwufake eshubhuni ye-DNA ekhipha isampula bese uyixuba ngokulinganayo.
1.2 Iyalayisha
Thatha u-20μL we-reagent yokuhlanganisa futhi uyengeze ku-reagent yokuthola i-MPV, engeza u-5μL wesampula ecutshunguliwe ngenhla (Ukulawula okuhle nokulawula okungalungile kuzocutshungulwa ngokuhambisana namasampuli), vala i-tube cap, uyibeke phakathi ku-2000rpm ngo-10. imizuzwana.
2. Ukukhulisa i-PCR
2.1 Layisha ipuleti/amashubhu e-PCR alungisiwe ensimbini ye-fluorescence PCR, Ukulawula okungekuhle kanye nokulawula okuhle kuzosethelwa ukuhlolwa ngakunye.
2.2 Ukulungiselelwa kwesiteshi seFluorescent:
1) Khetha isiteshi se-FAM ukuze uthole i-MPV;
2) Khetha isiteshi se-HEX/VIC ukuze uthole ukutholwa kofuzo lwangaphakathi;
3.Ukuhlaziywa kwemiphumela
Setha umugqa wesisekelo ngaphezu kwephoyinti eliphakeme kakhulu lejika le-fluorescent lesilawuli esinegethivu.
4.Ukulawulwa kwekhwalithi
4.1 Ukulawula okungekuhle: Alikho inani le-Ct elitholwe ku-FAM, i-HEX/VIC isiteshi, noma i-Ct＞40;
4.2 Ukulawula okuhle: Ku-FAM, HEX/VIC channel, Ct≤40;
4.3 Lezi zidingo ezingenhla kufanele zaneliswe esivivinyweni esifanayo, ngaphandle kwalokho imiphumela yokuhlolwa ayivumelekile futhi isilingo sidinga ukuphindwa.
【Sika inani】
Isampula libhekwa njengelihle uma: Ukulandelana kwethagethi Ct≤40, Igene yokulawula yangaphakathi Ct≤40.
【Ukuchazwa kwemiphumela】
Uma isilawuli sekhwalithi sesidlulisiwe, abasebenzisi kufanele bahlole ukuthi ingabe ikhona yini ijika lokukhulisa isampula ngalinye esiteshini se-HEX/VIC, uma likhona futhi nge-Ct≤40, ibonise uhlobo lofuzo lokulawula lwangaphakathi lukhuliswe ngempumelelo futhi lokhu kuhlola okuthile kuvumelekile. Abasebenzisi bangaqhubekela ekuhlaziyweni okulandelayo:
3.Kumasampuli anokukhulisa ufuzo lokulawula kwangaphakathi kwehlulekile (HEX/VIC
isiteshi, i-Ct＞40, noma alikho ijika lokukhulisa), umthamo weViral ophansi noma ukuba khona kwe-PCR inhibitor kungaba isizathu sokwehluleka, ukuhlolwa kufanele kuphindwe kusukela eqoqweni lesampula;
4.Kumasampula amahle kanye negciwane elikhulisiwe, imiphumela yokulawula kwangaphakathi ayithinti;
Kumasampuli ahlolwe ukuthi akanayo, isilawuli sangaphakathi sidinga ukuhlolwa ukuthi unayo ngaphandle kwalokho umphumela usuwonke awuvumelekile futhi ukuhlolwa kudinga ukuphindwa, kusukela esinyathelweni sokuqoqwa kwesifanekiso.
Ulwazi Lombukiso
Isitifiketi Sokuhlonishwa
Iphrofayela Yenkampani
Thina, i-Hangzhou Testsea Biotechnology Co., Ltd yinkampani ekhula ngokushesha yobuchwephesha be-biotechnology ekhethekile ekucwaningeni, ekuthuthukiseni, ekukhiqizeni nasekusabalaliseni amakhithi okuhlola e-in-vitro diagnostic(IVD) athuthukile namathuluzi ezokwelapha.
Indawo yethu yi-GMP, ISO9001, kanye ne-ISO13458 futhi sinemvume ye-CE FDA. Manje sesibheke ngabomvu ukubambisana nezinye izinkampani zaphesheya ukuze sithuthukiselane.
Sikhiqiza ukuhlolwa kwenzalo, ukuhlolwa kwezifo ezithathelwanayo, ukuhlola ukusetshenziswa kabi kwezidakamizwa, ukuhlola umaka wenhliziyo, ukuhlolwa komaka isimila, ukuhlolwa kokudla nokuphepha kanye nokuhlolwa kwezifo zezilwane, ngaphezu kwalokho, uhlobo lwethu lwe-TESTSEALABS lwaziwa kakhulu ezimakethe zasekhaya neziphesheya kwezilwandle. Izinga eliphezulu nezintengo ezivumayo zisenza sikwazi ukuthatha amasheya asekhaya angaphezu kuka-50%.
Inqubo Yomkhiqizo
1.Lungiselela
2.Ikhava
3.Ulwelwesi oluphambanayo
4.Sika umucu
5.Umhlangano
6.Pakisha izikhwama
7. Vala izikhwama
8.Pakisha ibhokisi
9.Encasement
Vimbela Inhlekelele Entsha: Lungiselela Manje Njengoba I-Monkeypox Isabalala
Ngo-Agasti 14, i-World Health Organization (WHO) yamemezela ukuthi ukuqubuka kwemonkeypox kuhlanganisa "Isimo Esiphuthumayo Sezempilo Yomphakathi Sokukhathazeka Kwamazwe Ngamazwe." Sekungokwesibili i-WHO ikhipha isexwayiso esiphezulu kakhulu mayelana nokuqubuka kwemonkeypox kusukela ngoJulayi 2022.
Njengamanje, ukuqubuka kwemonkeypox kusabalele kusuka e-Afrika kuya eYurophu nase-Asia, kanti amacala aqinisekisiwe abikwa eSweden nasePakistan.
Ngokwemininingwane yakamuva evela ku-Africa CDC, kulo nyaka, amazwe angu-12 angamalungu e-African Union abike izigameko ezingu-18,737 zemonkeypox, okuhlanganisa namacala aqinisekisiwe angu-3,101, amacala asolwayo angu-15,636, kanye nokufa kwabantu abangu-541, nezinga lokufa elingu-2.89%.
01 Yini Inkawu?
I-Monkeypox (MPX) yisifo se-viral zoonotic esibangelwa igciwane lemonkeypox. Ingadluliselwa isuka ezilwaneni iye kubantu, kanye naphakathi kwabantu. Izimpawu ezijwayelekile zihlanganisa umkhuhlane, ukuqubuka, kanye ne-lymphadenopathy.
Igciwane lemonkeypox lingena ngokuyinhloko emzimbeni womuntu ngolwelwesi lwamafinyila kanye nesikhumba esiphukile. Imithombo yokutheleleka ihlanganisa amacala emonkeypox namagundane angenwe yilesi sifo, izinkawu, nezinye izinkawu ezingezona abantu. Ngemva kokutheleleka, isikhathi sokufukamela siyizinsuku ezi-5 kuya kwezingu-21, ngokuvamile ziyizinsuku eziyisi-6 kuye kweziyi-13.
Nakuba abantu abaningi besengozini yokuthola igciwane lemonkeypox, kunezinga elithile lokuvikela inkawu kulabo abagonyelwe ingxibongo, ngenxa yokufana kofuzo kanye ne-antigenic phakathi kwamagciwane. Njengamanje, i-monkeypox isakazeka ikakhulukazi phakathi kwamadoda aya ocansini namadoda ngokuthintana ngokocansi, kuyilapho ingozi yokutheleleka kubantu abaningi ihlala iphansi.
02 Kwehluke Kanjani Lokhu Kuqubuka Kwenkawu?
Kusukela ekuqaleni konyaka, uhlobo oluyinhloko lwegciwane lemonkeypox, "i-Clade II," libangele ukuqubuka okukhulu emhlabeni jikelele. Okukhathazayo ukuthi, inani lamacala abangelwa "i-Clade I," elibi kakhulu futhi elinezinga eliphezulu lokufa, liyakhula futhi seliqinisekisiwe ngaphandle kwezwekazi lase-Afrika. Ukwengeza, kusukela ngoSepthemba wonyaka odlule, okuhlukile okusha, okuyingozi futhi okuthathelana kalula, "Clade Ib," isiqalile ukusabalala eDemocratic Republic of the Congo.
Okuqaphelekayo ngalesi sifo ukuthi abesifazane nezingane ezineminyaka engaphansi kweyi-15 yibona abathinteka kakhulu.
Idatha ikhombisa ukuthi ngaphezu kuka-70% wamacala abikiwe asezigulini ezineminyaka engaphansi kwe-15 ubudala, futhi phakathi kwezigameko ezibulalayo, lesi sibalo sikhuphukela ku-85%. Ngokuphawulekayo,izinga lokufa kwezingane liphakeme ngokuphindwe kane kunabantu abadala.
03 Iyini Ingozi Yokudluliselwa Kwenkawu?
Ngenxa yenkathi yezivakashi kanye nokusebenzisana okuvamile kwamazwe ngamazwe, ingozi yokudluliselwa kwegciwane lemonkeypox emngceleni ingase ikhule. Kodwa-ke, leli gciwane lisakazeka kakhulu ngokusondelana isikhathi eside, njengokuya ocansini, ukuthintana kwesikhumba, nokuphefumula eduze noma ukukhuluma nabanye, ngakho amandla alo okudlulisela kumuntu nomuntu abuthakathaka.
04 Uyivikela Kanjani I-monkeypox?
Gwema ukuya ocansini nabantu abangaziwa isimo sabo sempilo. Abahambi kufanele banake ukuqubuka kwemonkeypox emazweni nezifunda lapho beya khona futhi bagweme ukuthintana namagundane nezinkawu.
Uma ukuziphatha okuyingozi kakhulu kwenzeka, zibheke wena impilo yakho izinsuku ezingu-21 futhi ugweme ukusondelana nabanye. Uma izimpawu ezinjengokuqubuka, amabhamuza, noma imfiva zivela, funa usizo lwezokwelapha ngokushesha futhi wazise udokotela ngokuziphatha okufanele.
Uma ilungu lomndeni noma umngane kutholakala ukuthi unenkawu, thatha izinyathelo zokuzivikela, gwema ukusondelana nesiguli, futhi ungathinti izinto isiguli esizisebenzisile, njengezingubo, okokulala, amathawula, nezinye izinto zomuntu siqu. Gwema ukuhlanganyela amagumbi okugeza, futhi ugeze izandla njalo futhi ukhiphe umoya namagumbi.
I-Monkeypox Diagnostic Reagents
Ama-reagents okuxilongwa kwemonkeypox asiza ukuqinisekisa ukutheleleka ngokuthola ama-antigen egciwane noma amasosha omzimba, okwenza kube nezinyathelo ezifanele zokuzihlukanisa nezokwelashwa, futhi adlale indima ebalulekile ekulawuleni izifo ezithathelwanayo. Njengamanje, i-Anhui DeepBlue Medical Technology Co., Ltd. isungule ama-reagents okuxilonga imonkeypox alandelayo:
Ikhithi Yokuhlola I-Monkeypox Antigen: Isebenzisa indlela yegolide ye-colloidal ukuze iqoqe izifanekiso ezifana ne-oropharyngeal swabs, ama-nasopharyngeal swabs, noma ama-exudate esikhumba ukuze atholakale. Iqinisekisa ukutheleleka ngokuthola ukuba khona kwama-antigen egciwane.
Ikhithi Yokuhlola I-Monkeypox Antibody: Isebenzisa indlela yegolide ye-colloidal, namasampula afaka igazi eligcwele le-venous, i-plasma, noma i-serum. Iqinisekisa ukutheleleka ngokuthola amasosha omzimba akhiqizwa umzimba womuntu noma wesilwane ngokumelene negciwane lemonkeypox.
Ikhithi Yokuhlola I-Monkeypox Virus Nucleic Acid: Isebenzisa indlela ye-PCR ye-fluorescent yesikhathi sangempela, isampula ibe yi-lesion exudate. Iqinisekisa ukutheleleka ngokuthola i-genome yegciwane noma izingcezu ezithile zofuzo.
Imikhiqizo Yokuhlola I-Monkeypox ye-Testsealabs
Kusukela ngo-2015, izinto zokuxilonga i-monkeypox ze-Testsealabs ziye zaqinisekiswa kusetshenziswa amasampula egciwane langempela kumalabhorethri angaphandle futhi anikezwe isitifiketi se-CE ngenxa yokusebenza kwawo okuzinzile nokuthembekile. Lawa ma-reagents aqondise ezinhlotsheni zesampula ezihlukene, ahlinzeka ngamazinga ahlukene okuzwela kanye nokucaciswa, ahlinzeka ngokusekela okuqinile kokutholwa kokutheleleka kwemonkeypox nokusiza kangcono ekulawuleni ukuqubuka okuphumelelayo. Ukuze uthole ulwazi olwengeziwe mayelana nekhithi yethu yokuhlola i-monkeypox, sicela ubuyekeze: https://www.tesselabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Inqubo yokuhlola
Ukusebenzisa i-swab ukuqoqa ubomvu kusuka ku-pustule, ukuxuba kahle ku-buffer, bese usebenzisa amaconsi ambalwa ekhadini lokuhlola. Umphumela ungatholakala ngezinyathelo ezimbalwa nje ezilula.
