I-monkeypox virus (mpv) ikhithi yokutholwa kwe-nucleic acid
Ukuqalisa
I-kit isetshenziselwa ukutholwa kwe-vitro okufanelekile kwamacala asolwayo e-monkeypox virus (MPV), amacala ahlanganisiwe namanye amacala adinga ukutholakala ukuthi atheleleke ngegciwane le-mokeypox.
Ikhithi isetshenziselwa ukuthola uhlobo lwe-F3l lwe-MPV kuma-Throat Swabs kanye nama-Nasal Swab Samples.
Imiphumela yokuhlola yaleli khithi yesethenjwa emtholampilo kuphela futhi akufanele isetshenziswe njengendlela kuphela yokuxilongwa kwemitholampilo. Kunconywa ukwenza ukuhlaziywa okuphelele kwesimo ngokuya ngomtholampilo wesiguli
ukuboniswa nezinye izivivinyo zelebhu.
Ukusetshenziswa okuhlosiwe
| Uhlobo lwe-assay | Swabs Throat Swabs and Nasal Swab |
| Uhlobo lokuhlola | -Qumbile |
| Isivivinyo sokuhlola | Bhr |
| Usayizi wepakethe | Ama-48TESTS / 1 Ibhokisi |
| Ukushisa okushisa | 2-30 ℃ |
| Impilo yeshelufu | Izinyanga eziyi-10 |
Isici Somkhiqizo
Isimiso
Le khithi ithatha ukulandelana okuthile kwe-MPV F3L Gene njengendawo okuhlosiwe. Ubuchwepheshe be-Pucle tightitive we-Fluorescence PREACTEAL NE-NUCLEIC APAST SAPPECEECTURCE Technology basetshenziselwa ukuqapha i-viral nucleic acid ngokusebenzisa ukuguqulwa kwesiginali ye-fluorescence yemikhiqizo ye-amplification. Uhlelo lokutholwa luhlanganisa ukulawulwa kwekhwalithi yangaphakathi, elisetshenziselwa ukuqapha ukuthi ngabe kukhona ama-PCR inhibitors kumasampula noma ngabe amaseli kumasampula athathwa, angavimba ngempumelelo isimo esingesihle esingesihle.
Izingxenye eziphambili
Ikhithi iqukethe ama-reagents wokucubungula izivivinyo ezingama-48 noma ukulawulwa kwekhwalithi, kufaka phakathi izinto ezilandelayo:
Reagent a
| Ibizo | Izingxenye eziphambili | Ubuningi |
| Ukutholwa kwe-MPV isiphendukeleko | Ishubhu yokuphendula iqukethe i-MG2 +, I-F3L gene / rnase p primer prime, I-Reaction Buffer, TAQ DNA Enzyme. | Izivivinyo ezingama-48 |
IsiphendukelekoB
| Ibizo | Izingxenye eziphambili | Ubuningi |
| I-MPV Ukulawula Okuhle | Equkethe isiqephu se-MPV kwelitshe | 1 ishubhu |
| I-MPV Ukulawulwa Okungalungile | Ngaphandle kwengxenye ye-MPV Target | 1 ishubhu |
| I-DNA Depers Reagent | I-reagent iqukethe i-tris, edta neTriton. | Ama-48pcs |
| Reconstiture reaglent | UDepp waphatha amanzi | I-5ML |
Qaphela: Izingxenye zezinombolo ezahlukahlukene ze-batch azikwazi ukusetshenziswa ngokushintshana
【Imibandela yokugcina kanye nempilo eshalofly】
1.Reagent A / B ingagcinwa ngo-2-30 ° C, futhi impilo yeshelufu izinyanga eziyi-10.
2.Sicela uvule isembozo se-test tube kuphela lapho usukulungele ukuhlolwa.
3.Ungasebenzisi amashubhu wokuhlola ngaphezu kosuku lokuphelelwa yisikhathi.
4.Ungasebenzisi ishubhu evuzayo yokuthola.
【Ithuluzi elisebenzayo】
Ilungele uhlelo olufanele uhlelo lokuhlaziya lwe-LC480 PCR, i-Gentier 48E-othomathikhi yokuhlaziya i-PCR HRY, uhlelo lokuhlaziya lwe-ABI7500 lwe-PCR.
【Izidingo zesampula】
Izinhlobo zesampula ze-1.pplicable: I-Throat Swabs amasampula.
Isixazululo se-2.Sampling:Ngemuva kokuqinisekiswa, kunconyelwa ukusebenzisa i-saline ejwayelekile noma i-virus yokulondolozwa kwe-tube ekhiqizwe yi-Hangzhou testsea biology yesampula yesampula.
I-Throat Swab:Sula i-geryngeal ye-pharyngeal tonsils kanye nodonga lwangemuva lwe-pharyngeal nge-swab yesampula eyindilinga elahlekile, igxunyekwe i-swab kwishubhu equkethe ikhambi lesampula le-3ml, lahla umsila, bese uqinisa ikhava yeshubhu.
3.Sample Ukulondolozwa nokulethwa:Amasampula okufanele ahlolwe kufanele ahlolwe ngokushesha okukhulu. Izinga lokushisa lezokuhamba kufanele ligcinwe ngo-2 ~ 8 ℃ .Izibonelo ezingahlolelwa kungakapheli amahora angama-24 zingagcinwa ku-2 ℃ ~ 8 ℃ futhi uma amasampula engatholakali kungakapheli amahora angama-24, kufanele agcinwe kuya -70 ℃ (Uma kungekho simo sokugcina esingu -70 ℃, kungagcinwa ku-20 ℃ okwesikhashana), gwema ukuphindaphinda
ukuqaqeka nokuncipha.
Iqoqo lesampuli eli-4.propeper, isitoreji, kanye nokuhamba kubalulekile ekusebenzeni kwalo mkhiqizo.
【Indlela yokuhlola】
Ukulungiswa kwe-1.SAMPLE ukucubungula nokungeziwe kwesampula
1.1 Ukucutshungulwa kwesampula
Ngemuva kokuxuba isixazululo esingenhla sesampula ngamasampula, thatha ama-30μL wesampula kwi-DNA Delether Repent reagent tuagent tuagent realent tuagent bese uyixube ngokulinganayo.
1.2 Ukulayisha
Thatha i-20μL yokubuyiselwa kabusha kwe-reconstitution bese uyifaka ku-realent yokutholwa kwe-MPV, engeza i-5μL yesampula esetshenzisiwe engenhla (ukulawulwa okuhle nokulawulwa okungalungile kuzocutshungulwa ngokufana namasampula), isembozo se-tube cap, i-2000rpm ye-10 imizuzwana.
2. I-PCR Amplification
2.1 Layisha ipuleti / amashubhu we-PCR alungiselelwe ku-Fluorescence PCRENT, ukulawula okungalungile nokulawulwa okuhle kuzosethwa ukuhlolwa ngakunye.
2.2 Ukuhlelwa kwesiteshi se-Fluorescent:
1) Khetha isiteshi se-Fam sokutholwa kwe-MPV;
2) Khetha isiteshi se-Hex / VIC sokutholwa kohlobo lwangaphakathi;
Ukuhlaziywa kwe-3.Reruults
Setha umugqa wesisekelo ngaphezulu kwendawo ephezulu kakhulu yejika le-fluorescent elingemihle.
4.Quality Control
4.1 Ukulawulwa Okungalungile: Alikho inani le-CT elitholwe ku-Fam, Hex / VIC ngesiteshi, noma i-CT> 40;
4.2 Ukulawulwa okuhle: Ku-Fam, Hex / VIC Channel, CT≤40;
4.3 Lezi zidingo ezingenhla kufanele zaneliseke ekuhlolweni okufanayo, uma kungenjalo imiphumela yokuhlola ayivumelekile futhi ukuhlolwa kudinga ukuphindwa.
【Sika inani】
Isampula ithathwa njenge-HIV
【Ukuhunyushwa Kwemiphumela】
Lapho ukulawulwa kwekhwalithi sekudlulile, abasebenzisi kufanele bahlole ukuthi ngabe kukhona i-Amplification Curve yesampula ngayinye esiteshini se-Hex / VIC, uma ngabe i-CT≤40, ibonise ukuthi i-CT≤40, ingabe lolu vivinyo oluthile luvumelekile. Abasebenzisi bangaqhubeka nokuhlaziywa kokulandela:
3.I-Samples nge-amplification ye-Internal Control Gene Yehlulekile (Hex / Vic
Isiteshi, i-CT> 40, noma i-Akhawunti ye-Amplification), umthamo wegciwane ophansi noma ubukhona be-PCR levibitor kungaba yisizathu sokwehluleka, ukuhlolwa kufanele kuphinde kubuye kusuka ekuqoqweni okucacisiwe;
4.Ngabika amasampula amahle negciwane lesiko, imiphumela yokulawulwa kwangaphakathi ayithinti;
Kwamasampula ahlolwe kahle, ukulawulwa kwangaphakathi kudinga ukuhlolwa okuhle ngaphandle kwalokho okulandelayo akuvumelekile futhi ukuhlolwa kudinga ukuphindwa, kusukela kusinyathelo sokuqoqwa kwe-specimen
Imininingwane yombukiso
Isitifiketi sokuhlonishwa
Iphrofayela Yenkampani
Thina, i-Hangzhou TestSea Biotechnology Co, Ltd iyinkampani ekhula ngokushesha nge-biotechnology ekhethekile ekucwaningeni, ekwakhekeni, ekwakheni nasekusabalalisa kwe-Advanced in-vitro Diagnostic (IVD) Kits nezinsimbi zokwelashwa.
Isikhungo sethu yi-GMP, iso9001, kanye ne-ISO13458 Certified futhi sinemvume ye-CDA FDA. Manje silindele ukubambisana nezinkampani zaphesheya kwezilwandle zentuthuko.
Siveza ukuhlolwa kokuzala, izivivinyo zezifo ezithathelwanayo, izivivinyo zokuhlukumeza izidakamizwa, ukuhlolwa kokumaka kwenhliziyo, ukuhlolwa kokumaka izimila, ukuhlolwa kwezifo zezilwane, ngaphezu kwalokho, izivivinyo zethu zomkhiqizo ziye zaziwa kakhulu kuzo zombili izimakethe zasekhaya naphesheya kwezilwandle. Amanani asezingeni eliphakeme kakhulu futhi avumayo asivumela ukuthi sithathe amasheya angama-50% amasheya asekhaya.
Inqubo Yomkhiqizo
1.Prepare
I-2.Cover
3..
4.Cut strip
Umbhalo
6.Pack the Pouches
7.Sealeas Pouches
8.Pack ibhokisi
9.Bament
Vikela inhlekelele entsha: Lungiselela Manje njengoba i-monkeypox isakazeka
Ngo-Agasti 14, iWorld Health Organisation (WHO) Ememezele ukuthi ukuqubuka kweMokeypox kwakha "isimo sezulu sikahulumeni ukukhathazeka kwamanye amazwe." Lesi yisikhathi sesibili esikhiphe izinga eliphakeme kakhulu lokuqwashisa mayelana nokuqubuka kweMokeypox kusukela ngoJulayi 2022.
Njengamanje, ukuqubuka kweMokeypox kusatshalaliswe e-Afrika eYurophu nase-Asia, amacala aqinisekisiwe abikwe eSweden nasePakistan.
Ngokwemininingwane yakamuva evela e-Afrika CDC, kulo nyaka, amazwe angamalungu e-Africa Union abike amacala angama-18 781 aqinisekisile, kanti amacala angama-5,636 asolwayo, anesilinganiso sokufa kuka-2.89%.
01 Yini i-monkeypox?
I-Monkeypox (MPX) yisifo se-soonotic esibangelwa yi-monkey. Ingadluliselwa ezilwaneni kubantu, kanye naphakathi kwabantu. Izimpawu ezijwayelekile zifaka umkhuhlane, ukuqubuka kanye ne-lymphadenopathy.
Igciwane leMokeypox ngokuyinhloko lingena emzimbeni womuntu ngokusebenzisa ulwelwesi lwama-mucous kanye nesikhumba esiphukile. Imithombo yokutheleleka ifaka amacala ama-monkeypox namagundane athelelekile, izinkawu, nezinye izisekelo ezingezona ezenziwa ngabantu. Ngemuva kokutheleleka, isikhathi sokufakwa kokufakwa yizinsuku ezi-5 kuye kwezingama-21, ngokuvamile izinsuku ezi-6 kuye kwezingu-13.
Yize inani elijwayelekile litholakala nge-monkes virus, kunesivikelo esithile sokuvikelwa ku-monkeypox kulabo abagonyelwe i-infypox, ngenxa yokufana kwefuzo kanye ne-antigenic phakathi kwamagciwane. Njengamanje, i-monkeypox ngokuyinhloko isakazeka phakathi kwamadoda alale nabesilisa ngokuxhumana ngokocansi, ngenkathi ubungozi bokutheleleka kwabantu abajwayelekile buhlala buphansi.
02 Ngabe lokhu kuqubuka kanjani kweMokeypox?
Kusukela ekuqaleni konyaka, uhlobo olukhulu lwe-monkey virus, "clade II," kubangele ukuqubuka okukhulu emhlabeni wonke. Ngokwezifiso, ingxenye yamacala adalwa yi- "Clade I," okunzima kakhulu futhi kunesilinganiso esiphakeme sokufa, kuyanda futhi kuqinisekisiwe ngaphandle kwezwekazi lase-Afrika. Ngokwengeziwe, kusukela ngoSepthemba nyakenye, kungukuhluka okuthe xaxa, okuguqukayo futhi okuguqukayo kalula, "I-Clade IB, "sekuqalile ukusakazeka eDemocratic Republic of the Congo.
Isici esiphawuleka salokhu kuqubuka ukuthi abesifazane nezingane abangaphansi kweminyaka eyi-15 bathinteka kakhulu.
Idatha ikhombisa ukuthi amacala angaphezu kwama-70% abikiwe asezigulini ezingaphansi kweminyaka eyi-15, naphakathi kwamacala abulale, lesi sibalo sikhuphukela ku-85%. Ikakhulukazi,Izinga lokufa lezingane liphakeme kane kunabantu abadala.
03 Buyini ubungozi bokuhanjiswa kweMokeypox?
Ngenxa yesizini yezivakashi nokusebenzisana okujwayelekile kwamanye amazwe, ingozi yokudluliswa komngcele we-cross-umngcele we-monkey virus ingakhuphuka. Kodwa-ke, leli gciwane lisakazeka ngokuxhumana isikhathi eside, njengokusebenza ngokocansi, ukuxhumana kwesikhumba, kanye nokuphefumula okusondelene naphakathi noma ukukhuluma nabanye, ngakho-ke amandla okuhambisa abantu awomuntu abuthakathaka kakhulu.
04 Ungayivimbela kanjani i-monkeypox?
Gwema ukuxhumana ngocansi nomuntu ngamunye isimo sakhe sempilo asaziwa. Abahambi kufanele banake ukuqubuka kwe-monkeypox emazweni abo nasezifundeni futhi bagweme ukuxhumana nama-rodents kanye nama-prim.
Uma ukusebenza ngobungozi obukhulu kwenzeka, ukuziqapha impilo yakho izinsuku ezingama-21 futhi kugweme ukuxhumana okusondelene nabanye. Uma kuvela izimpawu ezifana nokuqhuma, ama-blisters, noma umkhuhlane kuvela, funa ukwelashwa ngokushesha futhi wazise udokotela ngokuziphatha okufanele.
Uma ilungu lomndeni noma umngani etholakala nge-monkeypox, thatha izinyathelo zokuzivikela, gwema ukuxhumana okusondelene nesiguli, futhi ungathinti izinto isiguli esizisebenzisile, njengezingubo, imibhede, amathawula nezinye izinto zomuntu siqu. Gwema ukwabelana ngezindlu zokugezela, futhi ugeze njalo izandla nezinsuku zangena ngaphakathi.
I-Monkeypox Diagnostic reagents
I-Monkeypox Diagnostic reagents isiza ukuqinisekisa ukutheleleka ngokuthola ama-antigen amagciwane noma ama-antibodies, ukunika amandla izindlela ezifanele zokuhlukaniswa kanye nokwelashwa, nokudlala indima ebalulekile ekutholeni izifo ezithathelwanayo. Njengamanje, i-Anhui Deplue Medical Technology Co, Ltd. ithuthukise ama-reagents alandelayo weMokeypox:
I-Monkeypox Antigen Test Kit: Isebenzisa indlela yegolide yeColloidal ukuqoqa ama-specimens anjenge-Oropharyngeal Swabs, ama-nasopharyngeal swabs, noma isikhumba sikhipha ukutholwa. Kuqinisekisa ukutheleleka ngokuthola ubukhona be-antigens yegciwane.
I-Monkeypox antibody test kit: Isebenzisa indlela yegolide ye-colloidal, ngamasampula afaka igazi lonke, i-plasma, noma i-serum. Kuqinisekisa ukutheleleka ngokuthola ama-antibodies akhiqizwa umzimba womuntu noma wezilwane ngokumelene ne-monkeyy virus.
I-MonkeyyPox Virus Nucleic Acid Test Test Kuqinisekisa ukutheleleka ngokuthola i-genome ka-virus noma izingcezwana ezithile zofuzo.
Imikhiqizo yokuhlola 'ye-monkeypox
Kusukela ngonyaka ka-2015, ama-testseabubs 'Deagnostic reagents aqinisekisiwe asebenzisa amasampula angempela amagciwane ezindaweni zokuhlala aphesheya futhi abelokhu eqinisekiswe yiCE ngenxa yokusebenza kwawo okuzinzile nokuthembekile. Lezi reagents zihlose izinhlobo ezahlukene zesampula, zinikeza ukuzwela okuhlukahlukene kanye namazinga okucacisa, ukuhlinzeka ngokusekelwa okuqinile kokutholwa ukutheleleka kwe-monkeypox nokusiza kangcono ukulawulwa okuvela kahle. Imininingwane eminingi nge-monkeypox test kit, sicela ubuyekeze: https://www.testseabubs.com/onkeypox-virus-chlit-detection-kit-uct-
Inqubo yokuhlola
Usebenzisa i-swab ukuqoqa i-pus kusuka ku-pustule, uyixube kahle kuBuffer, bese usebenzisa amaconsi ambalwa ekhadini lokuhlola. Umphumela ungatholakala ngezinyathelo ezimbalwa nje ezilula.
