I-monkeypox antigen test cassette (serum / plasma / swabs)

Incazelo emfushane:

Monkeypoxyisifo se-viral soorotic esibangelwa yi-Igciwane leMokeyypox, okungokwe-Uhlobo lwe-OrthopoxvirusyeUmndeni wePoxviridae. Ngenkathi ifana nengxibongo, i-monkeypox ngokuvamile ayinzima futhi inesilinganiso esiphansi sokufa. Leli gciwane litholwe okokuqala1958Ezinkalweni zelebhu (yingakho igama), kepha manje saziwa ngokuyinhloko lithinta izinduku nezinye izilwane. Lesi sifo sabikwa okokuqala kubantuNgo-1970kuIDemocratic Republic of Congo.

IMokeypox ingadluliselwa kubantu ngokusebenzisa:

Ukuxhumana ngqonezilwane ezithelelekile (isib. Ukuphatha i-bushmeat).
Ukudluliselwa komuntu kuya kumuntuNge-Drouplet Droplets, thintana eduze namanzi omzimba, noma izilonda zesikhumba.
Izinto ezidlulisa isifo(izinto ezingcolile noma indawo).

Izimpawu ze-monkeypox kubantu zifana naleziingxivula, kufaka phakathi:

Imfiva
Ukuphathwa yikhanda
Izinhlungu zemisipha
Ukudinwa
I-rasheps, ngokujwayelekile ukuqala ebusweni nokusakazeka kwezinye izingxenye zomzimba, kuvame ukuba yize kuzindilinga ezigcwele uketshezi (POX).

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Imininingwane Yomkhiqizo:

Ukuzwela okuphezulu nokucaciswa
Isivivinyo senzelwe ukuhlinzeka ngokutholwa okunembileI-monkeypox virus antigens noma ama-antibodies, nge-Cross-Real-Reactivity encane namanye amagciwane afanayo.
Imiphumela Esheshayo
Imiphumela iyatholakala ngaphakathiImizuzu engu-15-20, okwenza kube yinto elungele ukwenza izinqumo okusheshayo ngaphakathiIzilungiselelo Zomtholampilonoma ngesikhathi sokuqubuka.
Ukusebenziseka kalula
Isivivinyo sinobungane futhi asidingi ukuqeqeshwa okukhethekile noma imishini. Ilungele ukusetshenziswa ngabasebenzi bezempilo kuzilungiselelo ezahlukahlukene, kufaka phakathiamakamelo aphuthumayo, Imitholampilo Engaphandle, futhiIzibhedlela zasensimini.
Izinhlobo zesampula eziguquguqukayo
Ukuhlolwa kuyahambelana neigazi lonke, umbuso, nomauhlobo, enikeza ukuguquguquka ekuqoqweni kwesampula.
Iyaphatheka futhi ilungele ukusetshenziswa kwensimu
I-compact design yesivivinyo yenza kube yinto elungele ukusetshenziswa kuyoamayunithi ezempilo ephathekayo, Izinhlelo Zokufinyelela Komphakathi, futhiIzimo Zokuphendula Ubhubhane.

Isimiso:

Le khasiI-monkeypox ngokushesha isivivinyo sekhithiisebenza ngomgomo weUkugeleza Kwe-Lateral Flow Immunochrotography, lapho ukuhlolwa kuthola khonaI-Monkeypox virus antigens or ama-antibodies. Inqubo imi kanjena:

Ukuqoqwa kwesampula
Ivolumu encane yeigazi lonke, umbuso, nomauhloboingeziwe kwisampula kahle yedivayisi yokuhlola. Isixazululo se-buffer sesisetshenziselwa ukwenza lula ukuhamba kwesampula.
Antigen-antibody action
Ikhasethi yokuhlola iquketheI-Recombivery Antigens or ama-antibodiesethize ku-monkey virus. Uma isampula liqukethe i-monkeypox virus-ekhethekileama-antibodies(I-IGM, IGG) nomaamasheshiUkusuka ekuthelelekeni okusebenzayo, zizobopha kwingxenye ehambisanayo ekuhlolweni kokuhlola.
Ukufuduka kwe-Chromatographic
Isampula ihamba kanye nolwelwesi ngenxa yesenzo se-capillary. Uma ama-antigens athile athile noma ama-antibodies akhona, azobopha umugqa wokuhlola (umugqa we-T), ekhiqiza iqembu elinombala obonakalayo. Ukunyakaza kwama-reagents kuqinisekisa ukwakheka kweUmugqa Wokulawula (C umugqa), okuqinisekisa ubuqiniso bokuhlolwa.
Umphumela wokutolika
- Imigqa emibili (i-T Line + C line):Umphumela omuhle, okubonisa ukuba khona kwe-monkeypox virus antigen noma ama-antibodies.
- Umugqa owodwa (c umugqa kuphela):Umphumela omubi, ukhombisa ukuthi alikho i-monkey virus antigen noma ama-antibodies atholakalayo.
- Awukho umugqa noma umugqa kuphela:Umphumela ongavumelekile, odinga ukubhekelwa kabusha.

Ukwakheka:

Ukwakheka	Inani	Ukucacisa
Uma	1	/
Hlola ikhasethi	25	Isikhwama se-foil ngasinye esivaliwe esine-foil equkethe idivaysi eyodwa yokuhlola ne-desiccant eyodwa
Ukukhishwa okuchithiwe	I-500μL * 1 tube * 25	I-Tris-Cl Buffer, i-NACL, NP 40, Proclin 300
Ithiphu yeCropper	/	/
Celeb	25	/

Inqubo yokuhlola:


1. Geza izandla zakho	2. Bheka okuqukethwe Kit ngaphambi kokuhlola, faka ukufaka iphakheji, i-cassette yokuhlola, i-buffer, i-swab.	3.Phuma ishubhu lokukhishwa endaweni yokusebenza.	I-4PEEL Off Aluminium Foil Faal kusuka phezulu kweshubhu lokukhishwa eliqukethe i-buffer yokukhishwa.

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Ukususa nge-swab ngaphandle kokuthinta ithiphu.Insiza yonke ithiphu ye-swab 2 kuya ku-3 cm ungene endaweni efanelekile ye- Kuku-mimnor. Hlikihla ingaphakathi le-Nostril ekuhambeni okuyindilinga izikhathi ezi-5 okungenani amasekhondi ayi-15, manje thatha i-swab efanayo yamakhala bese uyifaka kwenye inkazimulo ku-tical most amahlandla ama-15. Sicela wenze isivivinyo ngqo ngesampula futhi ungayenzi
shiya kumi.

6.Phumana i-swab e-tube yokukhishwa.Dota i-swab imizuzwana eyi-10, ujikeleze i-swab ngokumelene ne-tube yokukhishwa, ucindezela inhloko ye-swab ngokumelene ne-tube lapho ukhipha izinhlangothi ze-tube ukuze ukhiphe uketshezi oluningi ngangokunokwenzeka kusuka ku-swab.


7. Thatha i-swab kusuka kwiphakheji ngaphandle kokuthinta i-padding.	I-8.mix kahle ngokufaka phansi phansi kwe-tube.place 3 amaconsi empumputhe ebheke kahle kwisampula kahle yekhasethi yokuhlola.Funda umphumela ngemuva kwemizuzu eyi-15. QAPHELA: Funda umphumela kungakapheli imizuzu engama-20.Isitatimende sokuhlola siyanconywa.

Ukuhunyushwa Kwezindlela: