Ikhasethi Lokuhlola I-MonkeyPox Antigen (Serum/Plasma/Swabs)

Incazelo emfushane:

Inkawuyisifo se-viral zoonotic esibangelwa yi-Igciwane lemonkeypox, okungeka-Uhlobo lwe-OrthopoxviruskweUmndeni we-Poxviridae. Nakuba ifana nengxibongo, inkawu ngokuvamile ayibi kakhulu futhi inezinga lokufa eliphansi. Igciwane laqala ukutholwa ku1958ezimfeneni zaselabhorethri (yingakho igama), kodwa manje sekwaziwa ukuthi ngokuyinhloko ithinta amagundane nezinye izilwane. Lesi sifo sabikwa okokuqala kubantu1970kweIDemocratic Republic of Congo.

I-Monkeypox ingadluliselwa kubantu ngokusebenzisa:

Ukuthintana ngqonezilwane ezinegciwane (isb, ukuphatha inyama yehlathi).
Ukudluliselwa komuntu kumuntungamaconsi okuphefumula, ukuthintana eduze noketshezi lomzimba, noma izilonda zesikhumba.
Izinto ezidlulisa isifo(izinto ezingcolile noma izindawo ezingaphezulu).

Izimpawu zemonkeypox kubantu zifana nalezo zeingxibongo, okuhlanganisa:

Imfiva
Ikhanda elibuhlungu
Ubuhlungu bemisipha
Ukukhathala
Amarashi, ngokuvamile iqala ebusweni futhi isakazekele kwezinye izingxenye zomzimba, ngokuvamile ikhula ibe izilonda ezigcwele uketshezi (upokisi).

Thumela i-imeyili kithi Landa njenge-PDF

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane Yomkhiqizo:

Ukuzwela Okuphezulu kanye Nokucaciswa
Ukuhlolwa kuklanyelwe ukuhlinzeka ngokutholwa okunembile kweI-Monkeypox virus antigens noma amasosha omzimba, enokusabela okuncane okuphambene namanye amagciwane afanayo.
Imiphumela Esheshayo
Imiphumela iyatholakala ngaphakathi15-20 imizuzu, okuyenza ilungele ukwenza izinqumo ngokusheshaizilungiselelo zomtholampilonoma ngesikhathi sokuqubuka.
Ukusebenziseka kalula
Ukuhlolwa kusebenziseka kalula futhi akudingi ukuqeqeshwa okukhethekile noma okokusebenza. Ilungele ukusetshenziswa ochwepheshe bezokunakekelwa kwempilo kuzilungiselelo ezahlukahlukene, kufaka phakathiamagumbi ezimo eziphuthumayo, imitholampilo yeziguli, futhiizibhedlela zensimu.
Izinhlobo Ezihlukahlukene Zesampula
Ukuhlolwa kuyahambisanaigazi lonke, i-serum, nomaiplasma, enikeza ukuguquguquka ekuqoqweni kwesampula.
Iyaphatheka futhi Ilungele Ukusetshenziswa Kwenkundla
Idizayini ehlangene yokuhlolwa iyenza ilungele ukusetshenziswa kuyoamayunithi ezempilo eselula, izinhlelo zokufinyelela emphakathini, futhiizimo zokuphendula ubhubhane.

Isimiso:

II-Monkeypox Rapid Test Kitisebenza ngomgomo wei-lateral flow immunochromatography, lapho ukuhlolwa kuthola khona nomaI-Monkeypox virus antigens or amasosha omzimba. Inqubo imi kanje:

Iqoqo Lesampula
Umthamo omncane weigazi lonke, i-serum, nomaiplasmayengezwe kumthombo wesampula wedivayisi yokuhlola. Isixazululo sebhafa sibe sesisetshenziswa ukuze kube lula ukuhamba kwesampula.
Ukusabela kwe-Antigen-Antibody
Ikhasethi lokuhlola liqukethei-recombinant antigen or amasosha omzimbaeqondene ngqo negciwane leMonkeypox. Uma isampula iqukethe i-Monkeypox virus-specificamasosha omzimba(IgM, IgG) nomaama-antigenkusukela ekuthelelekeni okusebenzayo, zizobophezela engxenyeni ehambisanayo emgqeni wokuhlola.
Ukufuduka kweChromatographic
Isampula ihamba eduze kwe-membrane ngenxa yesenzo se-capillary. Uma ama-antigens aqondene ne-Monkeypox noma amasosha omzimba ekhona, azobophezela kulayini wokuhlola (umugqa we-T), akhiqize ibhande elinemibala ebonakalayo. Ukuhamba kwama-reagents nakho kuqinisekisa ukwakheka kwe-aulayini wokulawula (umugqa C), okuqinisekisa ukufaneleka kokuhlolwa.
Ukuhunyushwa komphumela
- Imigqa emibili (T line + C line):Umphumela omuhle, okhombisa ukuba khona kwe-Monkeypox virus antigen noma amasosha omzimba.
- Umugqa owodwa (umugqa C kuphela):Umphumela ongemuhle, okhombisa ukuthi ayikho i-antigen yegciwane le-Monkeypox noma amasosha omzimba.
- Awukho ulayini noma u-T kuphela:Umphumela ongavumelekile, udinga ukuhlolwa kabusha.

Ukwakhiwa:

Ukwakheka	Inani	Ukucaciswa
IFU	1	/
Hlola ikhasethi	25	Isikhwama ngasinye se-foil esivaliwe esiqukethe idivayisi eyodwa yokuhlola kanye ne-desiccant eyodwa
I-diluent yokukhishwa	500μL1 Ishubhu 25	Ibhafa ye-Tris-Cl, NaCl, NP 40, ProClin 300
Ithiphu ye-Dropper	/	/
I-Swab	25	/

Inqubo Yokuhlola:


1. Geza izandla zakho	2. Hlola okuqukethwe kwekhithi ngaphambi kokuhlolwa, hlanganisa nokufakwa kwephakheji, i-test cassette, i-buffer, i-swab.	3.Beka ishubhu yokukhipha endaweni yokusebenza.	4.Hlubula isivalo se-aluminium foil kusukela phezulu kweshubhu yokukhipha equkethe isigcinalwazi sokukhipha.

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5.Khipha ngokucophelela i-swab ngaphandle kokuthinta ichopho.Faka yonke ichopho le-swab kusuka ku-2 kuya ku-3 cm ekhaleni elingakwesokudla.Qaphela ukuphuka kwe-swab yamakhala.Ungakuzwa lokhu ngeminwe yakho lapho ufaka i-swab yamakhala noma hlola. yona emncane. Gcoba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana engu-15,Manje thatha i-swab yamakhala efanayo futhi uyifake kwenye ikhala.Gwaba ingaphakathi lekhala ngokunyakazisa isiyingi izikhathi ezi-5 okungenani imizuzwana eyi-15. Sicela wenze ukuhlolwa ngokuqondile nesampula futhi ungakwenzi
ayishiye imile.

6.Beka i-swab eshubhuni yokukhipha. Zungezisa i-swab cishe imizuzwana eyi-10, Zungezisa i-swab eshubhuni lokukhipha, ucindezela ikhanda le-swab phakathi kweshubhu ngenkathi ucindezela izinhlangothi zeshubhu ukuze ukhiphe uketshezi oluningi. ngangokunokwenzeka kusuka ku-swab.


7. Khipha i-swab ephaketheni ngaphandle kokuthinta i-padding.	8.Xuba kahle ngokuqhweba phansi kweshubhu.Beka amathonsi ama-3 esampula ngokuqondile emthonjeni wesampula wekhasethi lokuhlola.Funda umphumela ngemva kwemizuzu engu-15. Qaphela: Funda umphumela kungakapheli imizuzu engama-20. Ngaphandle kwalokho, kunconyelwa ukucela isicelo sokuhlolwa.

Ukuhunyushwa Kwemiphumela: