I-ICH-CPV-CDV IGG Test

I-canine ethelelekayo ye-hepatitis / i-purvo virus kanye ne-canine ditterserper virus (CDV).

Okuqukethwe Kit

Design kanye nomgomo

Kunezakhi ezimbili ezihlanganiswe kwi-cartridge ngayinye: ukhiye, ofakwe kanye ne-desiccant egumbini elingaphansi elivalwe nge-foil foil evikelayo, kanye nokuhlakulela izixazululo, ezifakwa ngokwahlukana ezinhlakeni eziphezulu ze-foil foil.

I-cartridge ngayinye iqukethe wonke ama-reagents adingekayo wokuhlola okukodwa kwesampula. Kafushane nje, lapho ukhiye ufakwa ungene futhi ufakwe imizuzu embalwa egumbini eliphezulu 1, lapho kufakwe isampula yegazi, amasosha omzimba athile e-IGG ekhasini legazi elihlanjululwe, uma ekhona, azobopha i-ICH, CPV noma I-CDV ephindaphindwayo ama-antigens ayihlanganisiwe ezindaweni ezihlukile ze-discrete kukhiye ofakiwe. Lapho-ke ukhiye uzodluliselwa ezingxenyeni eziphezulu ezisele ngezikhathi ezinqunyelwe isikhathi ngesinyathelo. Ama-antibodies athile ahlanganisiwe we-IGG azofakwa kwi-top 3, equkethe ama-anti-canine iGG enzyme conjugate kanye nemiphumela yokugcina eyethulwe njengezindawo eziluhlaza okwesibhakabhaka kukhiye zizothuthukiswa phezulu

Igumbi 6, eliqukethe i-substrate. Ngomphumela ogculisayo, kwethulwa izinyathelo zokugeza. Egumbini eliphezulu 2, i-IGG engakhokhwanga nezinye izinto ngaphakathi kwesampula yegazi izosuswa. Egumbini eliphezulu 4 no-5, okungabandakanywa noma

Isihlanganisi se-anti-canine iGG enzyme sisuswe kahle. Ekupheleni, egumbini eliphezulu 7, i-chromosome eyeqile yathuthukiswa kusuka ku-substrate kanye ne-enzyme ye-enzyme eboshwe egumbini eliphezulu le-6 izosuswa. Ukuqinisekisa ubuqiniso bokusebenza, iprotheni yokulawula yethulwa endaweni engenhla kakhulu kukhiye. Ibala elikubala onsomi-okwesibhakabhaka kufanele libonakale ngemuva kokuqeda inqubo yokuhlola ephumelelayo.

Ukubeka

1. Gcina ikhithi ngaphansi kwesiqandisi esijwayelekile (2 ~ 8 ℃).

Ungandi ikhithi.

2. I-kit iqukethe izinto eziphilayo ezingasebenzi Ikhithi kumele isingathwe

kanye nokulahlwa ngokuhambisana nezidingo zokuhlanzeka zasendaweni.

Inqubo yokuhlola

Ukulungiselela ngaphambi kokwenza isivivinyo:

1. Letha i-cartridge yegumbi lokushisa (20 ℃ -30 ℃) bese ulibeka ebhentshini lomsebenzi kuze kube yilebula eshisayo odongeni lwe-cartridge liba umbala obomvu.

2.Phuma ephepheni lezicubu elihlanzekile ebhentshini lomsebenzi lokubeka ukhiye.

3.Prepare a 10μL Dispenser namathiphu we-10μL ajwayelekile wePipette.

4. Susa i-aluminium foil ephansi bese ukhipha ukhiye ngaphandle kwegumbi elingaphansi le-cartridge ephepheni lezicubu ezihlanzekile.

5 Thepha i-cartridge kancane ukuze uqiniseke ukuthi izixazululo ezinhlanganisweni eziphezulu zibuyela phansi ziye ezansi.

Ukwenza isivivinyo:

I-1.Umpula i-foil evikela izingxenye eziphezulu ngokucophelela nge-forefinger kanye nesithupha kusuka kwesokunxele kwesokudla kuze kube yilapho kuveza kuphela igumbi eliphezulu 1.

I-2.obtain isampula yegazi elihloliwe eline-dispenser setha usebenzisa ithiphu evamile ye-10μL pipette.

Ngokuhlola i-serum noma i-plasma isebenzisa i-5μL.

Ngokuhlola konke kusetshenziswe igazi eliphelele le-10μL.

I-Edta noma amashubhu ama-anticoaagulant amashubhu anconyelwa i-plasma nokuqoqwa kwegazi lonke.

3. Faka amasampula egumbini eliphezulu 1

4.Khetha ukhiye ngumnikazi we-Key's ophethe i-forefinger kanye nesithupha ngokucophelela bese ufaka ukhiye egumbini eliphezulu 1 wena). Bese uxuba bese ume ukhiye egumbini eliphezulu 1 imizuzu engu-5.

I-5

6

7.Ukunciphisa i-foil evikelayo ngokuqhubekayo ubheke kwesokudla kuze kube yilapho kuphela ukuveza i-centur 4. Thatha ukhiye ngumnikazi bese ufaka ukhiye egumbini eliveziwe 4 umzuzu o-1.

8.Ukunciphisa i-foil evikelayo ngokuqhubekayo ubheke kwesokudla kuze kube yilapho kuphela ukuveza i-centur 5. Thatha ukhiye ngumnikazi bese ufaka ukhiye egumbini eliphezulu eliveziwe 5.

I-9.Khumbula i-foil evikelayo ngokuqhubekayo ubheke kwesokudla kuze kube yilapho kuphela ukuveza i-centur 6. Thatha ukhiye ngumnikazi bese ufaka ukhiye egumbini eliveziwe 6 imizuzu emi-5.

I-10.Chuma i-foil evikelayo ngokuqhubekayo ubheke ngakwesokudla kuze kube yilapho kuphela ukuveza i-centur 7. Thatha ukhiye ngumnikazi bese ufaka ukhiye egumbini eliphezulu le-7.

11. Thatha ukhiye ngaphandle kwegumbi eliphezulu 7 bese ulivumele ephepheni lezicubu cishe imizuzu emi-5 ngaphambi kokufunda imiphumela.

Amanothi:

Ungathinti uhlangothi lwe-froming lokuphela kwangaphambili kwesihluthulelo, lapho ama-antigen kanye namaprotheni okulawula angasebenzi (ukuhlolwa nokulawula indawo).

Gwema ukuklwebheka isivivinyo nokulawula indawo ngokuncika kolunye uhlangothi olubushelelezi lokuphela kwangaphambili kwesihluthulelo kudonga elingaphakathi lwegumbi ngalinye eliphakeme ngenkathi lixuba.

Ngokuxuba, ukuphakamisa izikhathi eziyi-10 nokwehlisa ukhiye egumbini ngalinye eliphezulu kuyanconywa.

Veza kuphela indawo elandelayo ephezulu ephezulu ngaphambi kokudlulisa ukhiye.

Uma kunesidingo, faka amalebula wezilwane ezinikeziwe zokuhlolwa kwesampula okungaphezu kweyodwa.

Imiphumela yokuhlola yokuhumusha

Hlola izindawo eziholele kukhiye nge-Colorcale ejwayelekile

Okungavumelekile:

Alukho umbala obonakalayo ophuzi onsomi ovela endaweni yokulawula

-Phikisayo(-)

Alukho umbala obonakalayo onsomi ophuzi ovela ezindaweni zokuhlola

Okuhle (+)

Umbala obonakalayo onsomi ohlaza okwesibhakabhaka uvela ezindaweni zokuhlola

Izithako zama-antibodies athile athile angaboniswa ngamazinga amathathu