Ikhithi yokuhlola ye-ICH-CPV-CDV IgG

Ikhithi yokuhlola ye-CANINE INFECTIOUS HEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY (ICH/CPV/CDV IgG) iklanyelwe ukuhlola isilinganiso esincane sokulinganisa amazinga e-IgG antibody yenja ye-Canine Infectious Hepatitis Virus (ICH), Canine (CPV Parvo) kanye neCanine Distemper Virus(CDV).

OKUQUKETHWE IKHITHI

UKWENZIWA KANYE NESIMISO

Kunezingxenye ezimbili ezipakishwe ku-cartridge ngayinye: Ukhiye, ofakwe kanye ne-desiccant engxenyeni engezansi evalwe nge-aluminium foil evikelayo, kanye nezixazululo ezikhulayo, ezifakwe ngokuhlukana ezingxenyeni eziphezulu ezivalwe nge-aluminium foil evikelayo.

I-cartridge ngayinye iqukethe zonke izinto ezidingekayo zokuhlola isampula eyodwa. Kafushane, lapho Ukhiye ushuthekwa futhi ufukanyelwa imizuzu embalwa engxenyeni ephezulu 1, lapho kufakwe khona isampula yegazi, amasosha omzimba athile e-IgG kusampula yegazi elihlanjululiwe, uma ekhona, azobophezela ku-ICH, CPV noma I-CDV recombinant antigens ayinyakazi ezindaweni ezihlukene ezihlukene Kukhiye ofakiwe. Bese Ukhiye uzodluliselwa ezingxenyeni ezisele eziphezulu ngezikhathi ezibekiwe isinyathelo ngesinyathelo. Amasosha omzimba e-IgG anqunyelwe ezindaweni azobhalwa ku-compartment 3 ephezulu, equkethe i-anti-canine IgG enzyme conjugate futhi imiphumela yokugcina ethulwe njengamachashaza ansomi-luhlaza okwesibhakabhaka ku-Key izothuthukiswa phezulu.

Igumbi 6, eliqukethe i-substrate. Ukuze uthole umphumela owanelisayo, izinyathelo zokugeza ziyethulwa. Engxenyeni ephezulu 2, i-IgG engakhawulelwe nezinye izinto ezingaphakathi kwesampula yegazi zizokhishwa. Ekamelweni eliphezulu 4 no-5, i-unbounded noma

I-conjugate ye-enzyme ye-anti-canine ye-IgG eyengeziwe izoqedwa ngokwanele. Ekugcineni, engxenyeni engaphezulu yesi-7, i-chromosome eyeqile eyakhiwe kusuka ku-substrate kanye ne-enzyme ehlanganisiwe ehlanganisiwe engxenyeni ephezulu yesi-6 izokhishwa. Ukuqinisekisa ukufaneleka kokusebenza, iphrotheni yokulawula yethulwa endaweni ephezulu kakhulu Kukhiye. Indawo enombala onsomi-oluhlaza okwesibhakabhaka kufanele ibonakale ngemva kokuqeda inqubo yokuhlola eyimpumelelo.

ISITOREJI

1. Gcina ikhithi ngaphansi kwesiqandisi esivamile (2~8℃).

UNGAYIQISHI IKHITHI.

2. Ikhithi iqukethe izinto zebhayoloji ezingacushiwe. Ikhithi kufanele iphathwe

futhi ilahlwe ngokuhambisana nezidingo zenhlanzeko zendawo.

INQUBO YOKUHLOLA

Ukulungiselela ngaphambi kokwenza ukuhlolwa:

1. Letha i-cartridge ekamelweni lokushisa (20℃-30℃） futhi uyibeke ebhentshini lomsebenzi kuze kube yilapho ilebula eshisayo odongeni lwekhatriji iba umbala obomvu.

2.Beka iphepha lethishu elihlanzekile ebhentshini lomsebenzi ukuze ubeke Ukhiye.

3.Lungiselela isikhiphi esingu-10μL namathiphu wepayipi ajwayelekile angu-10μL.

4. Khipha icwecwe le-aluminium elivikelayo elingaphansi bese uphonsa Ukhiye ngaphandle kwengxenye engezansi ye-cartridge ephepheni lethishu elihlanzekile.

5. Yima uqonde ikhathriji ebhentshini lomsebenzi bese uqinisekisa ukuthi izinombolo zekhompathimenti ephezulu zibonakala ohlangothini olufanele (izitembu zezinombolo ezifanele zibheke kuwe). Thepha i-cartridge kancane ukuze wenze isiqiniseko sokuthi izixazululo ezingxenyeni eziphezulu zibuyela emuva phansi.

Ukwenza ukuhlolwa:

1.Vula i-foil yokuvikela emagumbini angaphezulu ngokucophelela ngomunwe wangaphambili kanye nesithupha ukusuka kwesokunxele ukuya kwesokudla kuze kube KUPHELA uveza ingxenye ephezulu engu-1.

2.Thola isampula yegazi elihloliwe ngesethi ye-dispenser usebenzisa ithiphu ye-pipette engu-10μL evamile.

Ukuze uhlole i-serum noma i-plasma sebenzisa i-5μL.

Ukuze uhlole igazi lonke sebenzisa i-10μL.

Amashubhu e-EDTA noma i-heparin anticoagulant ayanconywa ku-plasma kanye nokuqoqwa kwegazi lonke.

3. Beka isampula endaweni ephezulu 1. Bese uphakamisa futhi wehlise i-dispenser plunger izikhathi eziningana ukuze uzuze ukuxutshwa (Isixazululo esiluhlaza okwesibhakabhaka esikhanyayo esiphundu lapho ukuxuba kubonisa idiphozi yesampula eyimpumelelo).

4.Khetha Ukhiye Ngesibambi Sokhiye ngomunwe wangaphambili nangesithupha ngokucophelela bese ufaka Ukhiye endaweni engu-1 ephezulu (qinisekisa uhlangothi oluqandayo lukakhiye olubheke kuwe, noma qinisekisa ukuthi isiyingi esiyindilinga kuMbambi singakwesokudla uma sibheke. wena). Bese uhlanganisa bese umisa Ukhiye endaweni ephezulu 1 imizuzu emi-5.

5. Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze kube KUPHELA uveza ingxenye 2. Thatha Ukhiye Kusibambi bese ufaka Ukhiye endaweni evulekile 2. Bese uxuba bese umisa Ukhiye engxenyeni ephezulu 2 iminithi elingu-1.

6. Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze kube KUPHELA uveza ikhompathimenti 3. Thatha Ukhiye Kusibambi bese ufaka Ukhiye endaweni evulekile 3. Bese uxuba bese umisa Ukhiye endaweni yesi-3 imizuzu emi-5.

7.Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze KUPHELA uveza ikhompathimenti 4. Thatha Isihluthulelo Sesibambi bese ufaka Ukhiye endaweni evulekile 4. Bese uxuba bese umisa Ukhiye engxenyeni engaphezulu yesi-4 iminithi elingu-1.

8.Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze kube KUPHELA ubeka obala ingxenye 5. Thatha Isihluthulelo Sesibambi bese ufaka Ukhiye endaweni evulekile 5. Bese uxuba bese umisa Ukhiye engxenyeni engaphezulu 5 iminithi elingu-1.

9.Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze kube KUPHELA uveza ingxenye 6. Thatha Ukhiye Kusibambi bese ufaka Ukhiye endaweni evulekile 6. Bese uxuba bese umisa Ukhiye engxenyeni engaphezulu yesi-6 imizuzu emi-5.

10.Vula i-foil yokuvikela ngokuqhubekayo uye kwesokudla kuze kube KUPHELA uveza ingxenye 7. Thatha Isihluthulelo Sesibambi bese ufaka Ukhiye endaweni evulekile 7. Bese uxuba bese umisa Ukhiye engxenyeni engaphezulu yesi-7 iminithi elingu-1.

11. Khipha ukhiye engxenyeni engaphezulu yesi-7 bese uyeke ukuthi yome ephepheni lethishu cishe imizuzu emi-5 ngaphambi kokufunda imiphumela.

Amanothi:

Ungathinti I-Frosting Uhlangothi Olungaphambili Lokuphela Kokhiye, lapho ama-antigen kanye nephrotheni yokulawula kungasebenzi (Isifunda Sokuhlola Nokulawula).

Gwema ukunwaya Isifunda Sokuhlola Nokulawula ngokuncika olunye Uhlangothi Olubushelelezi lwengxenye engaphambili kakhiye odongeni olungaphakathi lwekhompathimenti ngayinye ephezulu ngenkathi uxuba.

Ngokuxuba, izikhathi ezingu-10 ukuphakamisa nokwehlisa Ukhiye endaweni ngayinye ephezulu kuyanconywa.

KUPHELA Veza ingxenye ephezulu elandelayo ngaphambi kokudlulisa Ukhiye.

Uma kudingekile, namathisela amalebula ezilwane ezifuywayo anikeziwe ukuze uthole ukuhlolwa kwesampula engaphezu kweyodwa.

UKUTOLIWA IMIPHUMELA YOKUHLOLA

Hlola izindawo eziwumphumela kukhiye nge-ColorScale ejwayelekile

Okungavumelekile:

AKUKHO umbala onsomi noluhlaza okwesibhakabhaka obonakalayo ovela endaweni yokulawula

Okubi(-)

AKUKHO umbala onsomi noluhlaza okwesibhakabhaka obonakalayo ovela ezindaweni zokuhlolwa

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Umbala obonakalayo onsomi noluhlaza okwesibhakabhaka uvela ezindaweni zokuhlola

Ama-titters amasosha omzimba athile e-IgG angaboniswa ngamazinga amathathu