Testsealabs Monkey Pox Antigen Test Cassette (Swab)
1.I-Cassette isetyenziselwa ukufumanisa i-in vitro qualitative cases ezikrokrelekayo zeMonkeypox Virus (MPV), iimeko ezidibeneyo kunye nezinye iimeko ezifuna ukufunyaniswa ngenxa yosulelo lweMonkeypox Virus.
2.I-Cassette yi-chromatographic immunoassay yokukhangela umgangatho we-Monkey Pox antigen kwi-oropharyngeal swabs ukunceda ekuxilongeni usulelo lwe-Monkey Pox virus.
3.Iziphumo zovavanyo zale Cassette zezereferensi yeklinikhi kuphela kwaye akufunekanga isetyenziswe njengeyona nto iyodwa ikhrayitheriya yokuxilongwa kwezonyango.Kunconywa ukwenza uhlalutyo olubanzi lwemeko esekelwe kwimibonakaliso yekliniki yesigulane kunye nezinye iimvavanyo zebhubhoratri.
INTSHAYELELO
| Uhlobo lwesivavanyi | I-Oropharyngeal swabs |
| Uhlobo lovavanyo | Umgangatho |
| Izinto zovavanyo | Isithinteli sokutsalwa kwangaphambiliIswab enyumbaIndawo yokusebenzela |
| Ubungakanani bepakethi | Iimvavanyo ezingama-48/1 ibhokisi |
| Ubushushu bokugcina | 4-30°C |
| Beka ubomi kwishelufa | Iinyanga ezili-10 |
UMBONO WEMVELISO
Umgaqo
I-Monkey Pox Antigen Test Cassette yimbrane esemgangathweni esekelwe kwi-immunoassay yokubhaqa i-antigen ye-Monkey Pox kwi-oropharyngeal swab specimen.Kule nkqubo yovavanyo, i-anti-Monkey Pox antibody ayishukumi kumgca wovavanyo wesixhobo.Emva kokuba i-oropharyngeal swab specimen ifakwe kwi-specimen kakuhle, isabela kunye ne-anti-Monkey Pox antibody coated particles eziye zasetyenziswa kwi-specimen pad.Lo mxube ufuduka ngokwechromatographic ngobude bomcu wovavanyo kwaye unxibelelane ne-anti-Monkey Pox engashukumiyo.Ukuba i-specimen iqulethe i-antigen ye-Monkey Pox, umgca onemibala uya kubonakala kwindawo yovavanyo ebonisa umphumo omuhle.
AMANQAKU APHAMBILI
Ikhithi iqulethe ii-reagents zokuqhuba iimvavanyo ezingama-48 okanye ulawulo lomgangatho, kubandakanya la macandelo alandelayo:
①Anti-Monkey Pox antibody njengesixhobo sokubanjwa, enye i-anti-Monkey Pox antibody njengesixhobo sokubhaqa.
②I-IgG yebhokhwe echasene neMouse iqeshwe kwindlela yokulawula.
Iimeko zoGcino kunye noBomi beShelf
1.Gcina njengoko zipakishwe kwisingxobo esivaliweyo kwiqondo lobushushu begumbi okanye efrijini (4-30°C)
2. Uvavanyo luzinzile ukuya kutsho kumhla wokuphelelwa kwexesha oluprintiweyo kwingxowa evaliweyo.Uvavanyo maluhlale kwisingxobo esivaliweyo de lusetyenziswe.
3. MUSA UKUMKHENZA.Musa ukusebenzisa ngaphaya komhla wokuphelelwa.
Isixhobo Esisebenzayo
I-Monkey Pox Antigen Test Cassette yenzelwe ukusetyenziswa kunye ne-oropharyngeal swabs.
(Nceda wenze iswabhu yenziwe ngumntu oqeqeshelwe ubugqirha.)
IiMfuno zeSampulu
1.Iintlobo zeesampulu ezisebenzayo:I-Oropharyngeal swabs.Nceda ungabuyiseli iswazi kwisisongelo saso sokuqala sephepha.Ukufumana iziphumo ezilungileyo, i-swabs kufuneka ivavanywe ngokukhawuleza emva kokuqokelela.Ukuba akunakwenzeka ukuvavanya ngokukhawuleza, kunjalo
kucetyiswa ngamandla ukuba i-swab ifakwe kwityhubhu yeplastiki ecocekileyo, engasetyenziswanga
ebhalwe ngolwazi lwesigulane ukugcina ukusebenza kakuhle kunye nokuphepha ukungcoliseka okunokwenzeka.
2.Isisombululo sesampulu:Emva kokuqinisekiswa, kuyacetyiswa ukuba kusetyenziswe ityhubhu yogcino lweNtsholongwane eveliswe yi-Hangzhou Testsea biology kuqokelelo lwesampulu.
3. Ukugcinwa kwesampuli kunye nokuhanjiswa:Isampuli inokugcinwa ivalwe ngokuqinileyo kule bhubhu kwindawo yokushisa (15-30 ° C) ubuninzi beyure enye.Qinisekisa ukuba i-swab ihleli ngokuqinileyo kwi-tube kwaye i-cap ivalwe ngokuqinileyo.
Ukuba kulibaziseka ngaphezu kweyure enye, lahla isampuli.Isampuli entsha kufuneka ithathwe kuvavanyo.Ukuba iisampulu ziza kuthuthwa, kufuneka zipakishwe ngokwemigaqo yendawo yokuthutha i-agent ye-atiological.
Indlela yoVavanyo
Vumela uvavanyo, isampuli kunye ne-buffer ukufikelela kwiqondo lokushisa kwegumbi 15-30 ° C (59-86 ° F) phambi kokubaleka.
① Beka ityhubhu yokutsalwa kwindawo yokusebenzela.
② Khulula isitywina sefoyile ye-aluminiyam ukusuka phezulu kumbhobho wokutsalwa oqulathe
ityhubhu yotsalo equlethe isithinteli sokutsalwa.
③ Yenza i-oropharyngeal swab eyenziwa ngumntu oqeqeshelwe ubugqirha njenge
ichazwe.
④ Beka i-swab kwityhubhu yokukhupha.Jikelezisa i-swab malunga nemizuzwana ye-10
⑤ Susa i-swab ngokujikeleza ngokuchasene ne-vial yokukhupha ngelixa ucinezela macala
yevial ukukhulula ulwelo kwiswab.lahla ngokufanelekileyo i-swab.ngelixa ucinezela
intloko ye swab ngokuchasene ngaphakathi kwityhubhu yokutsalwa ukukhupha ulwelo oluninzi
ngokusemandleni ukusuka kwi-swab.
⑥ Vala ibhotile nge-cap oyinikiweyo kwaye utyhale ngokuqinileyo kwi-vial.
⑦ Xuba ngokucokisekileyo ngokuqhwanyaza emazantsi etyhubhu. Beka amathontsi amathathu esampuli
ngokuthe nkqo kwisampulu yefestile yekhasethi yovavanyo.Funda umphumo emva kwemizuzu eyi-10-15.Funda isiphumo kwimizuzu engama-20.Ngaphandle koko, ukuphindwa kovavanyo kucetyiswa.
Uhlalutyo lweziphumo
1.Okuhle:Kuvela imigca emibini ebomvu.Omnye umgca obomvu ubonakala kwindawo yokulawula (C) kunye nomgca obomvu kwindawo yokuvavanya (T).Uvavanyo luthathwa ngokuba lulungile ukuba kwakubonakala umgca ongenamandla.Ubunzima bomgca wokuvavanya bunokwahluka ngokuxhomekeke kugxininiso lwezinto ezikhoyo kwisampuli.
2.Ibi: Kuphela kwindawo yokulawula (C) umgca obomvu uvela, kwindawo yokuvavanya (T) akukho mgca
ibonakala.Isiphumo esibi sibonisa ukuba akukho antigens zeMonkeypox kwisampulu okanye ukuxinwa kwee-antigens kungaphantsi komda wokufumanisa.
3.Ayisebenzi: Akukho mgca obomvu uvela kwindawo yokulawula (C).Uvavanyo alusebenzi nokuba kukho umgca kwindawo yovavanyo (T).Umthamo wesampulu engonelanga okanye ukuphathwa ngendlela engalunganga zezona zizathu zinokwenzeka zokungaphumeleli.Phonononga inkqubo yovavanyo uze uluphinde uvavanyo ngekhasethi entsha yovavanyo.
Ulawulo lwemeko
Uvavanyo luqulethe umgca onemibala ovela kwindawo yokulawula (C) njengolawulo lwangaphakathi lwenkqubo.Iqinisekisa umthamo owaneleyo wesampuli kunye nokuphathwa ngokuchanekileyo.Imigangatho yolawulo ayibonelelwanga nale khithi.Nangona kunjalo, kucetyiswa ukuba ulawulo oluqinisekileyo nolungalunganga luvavanywe njengenkqubo efanelekileyo yaselabhoratri ukuqinisekisa inkqubo yovavanyo kunye nokuqinisekisa ukusebenza kovavanyo olufanelekileyo.
Izinto eziphazamisayo
Ezi zixhobo zilandelayo zavavanywa kunye neMonkey Pox yovavanyo olukhawulezayo lwe-antigen kwaye akukho ziphazamiso zibonwa.
