Uvavanyo lwezifo ze-VeestSEA Vavanya i-HIV 1/2 ikhithi yovavanyo ngokukhawuleza

Uvavanyo lwezifo ze-VeestSEA Vavanyi i-HIV

Inkcazo emfutshane:

Intsholongwane yamandla abantu (i-HIV)yintsholongwane ehlasela amajoni omzimba, ajolise ngqo kwiIiseli ze-CD4 +. Ukuba ishiywe inganyangwa, i-HIV inokukhokelela kwiI-gonodeficiency syndrome (AIDS), imeko apho amajoni omzimba aya konakala kwaye engakwazi ukulwa nosulelo kunye nezifo.

Intsholongwane kaGawulayo idluliselwaigazi, isidoda, I-vagiids, I-frits fluids, kwayeubisi lwebele. Ezona ndlela ziqhelekileyo zosulelo zibandakanya ukunxibelelana ngesondo okungakhuselekanga, ukwabelana nenaliti ezingcolisekileyo, kunye nosulelo lwabasetyhini ngexesha lokuzalwa komntwana okanye ukuncancisa.

Zimbini iintlobo eziphambili ze-HIV:

Intsholongwane kaGawulayo-1:Olona hlobo luxhaphakileyo nolubanzi lwe-HIV kwihlabathi jikelele.
Intsholongwane kaGawulayo-2:Kuyinto encinci, ifumaneka kwiNtshona ye-Afrika, kwaye ihambelana nokuqhubela phambili kancinci kwe-AIDS.

Ukufunyanwa kwangoko kunye nonyango ngeUnyango lwe-antiretroviral (ART)inokunceda abantu abane-HIV baphila ubomi obude, benempilo kwaye banciphise ubungozi bokuhambisa abanye.

Thumela i-imeyile kuthi Khuphela njenge-PDF

Iinkcukacha zemveliso

Iimpawu zeMveliso

Iinkcukacha zemveliso:

Ubuntununtunu obuphezulu kunye nobunye
Olu vavanyo lwenzelwe ukuba lubone zombini ii-HIV-1 kunye nee-antibodies ezi-2 ne-HIV, zinika iziphumo ezinokuthenjwa ngokusebenza komntu omncinci.
Iziphumo eziPhuculweyo
Iziphumo ziyafumaneka ngaphakathi kwemizuzu eli-15 ukuya kwengama-20, zinika amandla okwenza izigqibo zeklinikhi kunye nokunciphisa ixesha lokulinda kwizigulana.
Ukunciphisa ukusetyenziswa
Uyilo olulula nolusebenziseka lula, lufuna izixhobo okanye uqeqesho olukhethekileyo. Ilungele ukusetyenziswa kuzo zombini izicwangciso zeklinikhi kunye neendawo ezikude.
Iindidi zesampulu
Uvavanyo luyahambelana negazi liphela, i-serum, okanye iplasma, ukubonelela ngokuguquguquka kuqokelelo lwesampulu kunye nokwandisa uluhlu lwezicelo.
Ukuphathwa kunye nokusetyenziswa kwentsimi
I-Compact kunye nezantsi, ukwenza iKit yoVavanyo elifanelekileyo kwii-AtoTIT ze-POVIT, iikliniki zezempilo, kunye neenkqubo zokuhlolwa kobunzima.

Umgaqo:

Ukuqokelelwa kwesampulu
Umthamo omncinci we-serum, iplasma, okanye igazi liphela lisetyenziswa kwisampulu yequmrhu lovavanyo, elilandelwe ngokongezwa kwesisombululo se-buffer ukuqalisa inkqubo yovavanyo.
Unxibelelwano lwe-Antign-Antict
Olu vavanyo luqulethe iibhloksi eziphindaphindiweyo kuzo zombini i-HIV-1 ne-HIV-2, engafakwanga kwingingqi yovavanyo ye-membrane. Ukuba ii-antibodies ze-HIV (i-Igg, i-Igm, okanye zombini) zikho kwisampulu, ziya kubopha kwi-antigens kwi-membrane, zenza i-antign-anticleds.
Ukufuduka kweChromatographic
I-Antigen--Antioulters ihambahamba kwi-membrane ngesenzo se-capillary. Ukuba ii-antibodies ze-HIV zikhona, ububanzi buyabopha kumgca wovavanyo (umgca), buvelisa umgca obonakalayo obonakalayo. Ii-regegents ezisaleleyo zifuduke kumgca wokulawula (C ulayini) ukuqinisekisa ubunyani bovavanyo.
Isiphumo sokutolika
- Imigca emibini (t Line + C Umnxeba):Isiphumo esihle, esibonisa ubukho bee-HIV-1 kunye / okanye ii-antibodies ze-HIV.
- Umgca omnye (c umgca kuphela):Isiphumo esibi, sibonisa ukungabi nazimpawu ze-HIV.
- Akukho mgca okanye umgca kuphela:Isiphumo esingasebenziyo, sifuna uvavanyo oluphindaphindwayo.

Ukuqulunqwa:

Ukwakhiwa	Isixa	Ukucaciswa
Ukuba	1	/
Vavanya ikhasethi yekhasethi	1	Ingxowa nganye etywiniweyo enesixhobo esinye sovavanyo kunye nesidima esinye
Ukukhupha i-disent	I-500s * I-1 ye-tube * 25	I-Tris-Cl buffer, i-NACL, i-NP 40, iProtlin 300
I-Dropper Tiphu	1	/
Swab	1	/

Inkqubo yovavanyo:


1. Hlamba izandla zakho	2. Jonga iziqulatho zeKhithi ngaphambi kovavanyo, zibandakanya ukufaka iphakheji, i-Cassette yovavanyo, i-buffer, i-swab.	3.Phakamisa ityhubhu yokukhupha kwindawo yokusebenza.	4.Umcimilili we-Aluminium Foil ovela phezulu kwityhubhu yokukhupha equlethe i-buffer yokukhupha.

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5. Ngokuyisela i-swab ngaphandle kokuchukumisa i-TIP.CECTE inqaku lonke le-swab 2 ukuya kwi-3 cm ukuya kwi-nostril swabl swab. kwi-mimnor. Hlikihla ngaphakathi kwempumlo kwi-Istrings kwiintshukumo ezi-5 ubuncinci imizuzwana eli-15, ngoku thatha i-swab efanayo kwaye uyifake i-nostrils. Nceda wenze uvavanyo ngqo kwisampulu kwaye ungabi
yiyeke imi.

6. Indawo ye-swab kwi-cube yokukhupha. I-Swab malunga nemizuzwana eli-10, ijikeleze i-swab ngokuchasene ne-tybhu ye-tybhu ngelixa inyibilika ityhubhu yokukhulula ulwelo ngokusemandleni e-swab.


7. Khupha i-swab ukusuka kwiphakheji ngaphandle kokuchukumisa i-padding.	8.Mix ngokucokisekileyo ngokufihla amazantsi e-tube.ble 3 amaconsi emo yesampulu ethe nkqo kwi-capsette yovavanyo.reat isiphumo emva kwemizuzu eli-15. Qaphela: Funda isiphumo ngaphakathi kwemizuzu engama-20.OMezinye, kuyacetyiswa uvavanyo lovavanyo.

Iziphumo: