Ikhithi yoVavanyo oluKhawulezayo lweCandelo leMveliso yeegusha (iNdlela yeGolide yeColloidal)

Izalathiso kunye nomthamo]
Beka i-reagent kunye nesampuli kwindawo yokushisa (10 ~ 30 ° C) imizuzu eyi-15-30. Uvavanyo kufuneka luqhutywe kwindawo yokushisa (10 ~ 30 ° C) kunye nomswakama obuninzi (umswakama ≤70%) kufuneka ugwenywe. Indlela yokuvavanya ihlala ihambelana phantsi kweemeko ezahlukeneyo zokushisa kunye nokufuma.
1.Ukulungiselela iSampuli
1.1 Ukulungiswa kweSampulu yeTissue engamanzi ukusuka kwiMeat Surface
(1) Sebenzisa i-swab ukufunxa i-tissue fluid ukusuka kumphezulu wesampuli ukuba ihlolwe, emva koko ucwilise i-swab kwisisombululo sokukhupha imizuzwana ye-10. Gxuma ngokucokisekileyo phezulu nasezantsi, ekhohlo nasekunene, imizuzwana eyi-10-20 ukunyibilikisa isampuli kwisisombululo kangangoko kunokwenzeka.
(2) Susa i-cotton swab, kwaye ulungele ukusebenzisa isampuli yolwelo.
1.2I-Meat Chunk Tissue Ukulungiswa kweSampuli
(1) Usebenzisa ipere yesikere (engabandakanyi), sika iqhekeza lenyama le-0.1g (malunga nobukhulu bembotyi yesoya). Yongeza i-chunk yenyama kwisisombululo sokukhupha kwaye udibanise imizuzwana ye-10. Sebenzisa i-swab ukuze ucinezele inyama ye-5-6 amaxesha, uvuselele ngokucokisekileyo, phantsi, ekhohlo, kwaye ekunene imizuzwana eyi-10-20. Unako ke ukusebenzisa ulwelo isampulu.
2.Amanyathelo okhuseleko
(1) Le rejenti yenzelwe kuphela uvavanyo lwenyama ekrwada okanye izinto zokutya ezingaphekwanga.
(2) Ukuba kufakwe ulwelo oluncinane kakhulu kwikhadi lovavanyo, ii-negatives ezibubuxoki okanye iziphumo ezingasebenziyo zinokuvela.
(3) Sebenzisa idropper/pipette ukulahla ngokuthe nkqo ulwelo lovavanyo kwisampulu yomngxuma wekhadi lovavanyo.
(4) Thintela ukungcoliseka phakathi kweesampuli ngexesha lokuthatha iisampulu.
(5) Xa usebenzisa isikere ukusika inyama, qinisekisa ukuba izikere zicocekile kwaye azinasulelo kwimvelaphi yezilwanyana. Isikere sinokucocwa kwaye siphinde sisetyenziswe amaxesha amaninzi.
[Utoliko lweziphumo zovavanyo]
Okuhle (+): Kuvela imigca emibini ebomvu. Umgca omnye ubonakala kwindawo yokuvavanya (T), kunye nomnye umgca kwindawo yokulawula (C). Umbala webhanti kwindawo yokuvavanya (T) unokuhluka ngamandla; nayiphi na imbonakalo ibonisa umphumo omuhle.
I-Negative (-): Kuphela ibhendi ebomvu ibonakala kwindawo yokulawula (C), kungekho bhanti ibonakala kwindawo yokuvavanya (T).
Ayisebenzi: Akukho bhanti ebomvu ibonakala kwindawo yolawulo (C), kungakhathaliseki ukuba ibhendi ibonakala kwindawo yovavanyo (T) okanye hayi. Oku kubonisa isiphumo esingasebenziyo; umtya omtsha wovavanyo kufuneka usetyenziselwe ukuvavanya kwakhona.
Isiphumo Esilungileyo sibonisa: Amacandelo emvelaphi yeegusha afunyenwe kwisampulu.
Isiphumo Esibi sibonisa: Akukho malungu emvelaphi yeegusha afunyenweyo kwisampulu.