I-SARS-CoV-2 Ikhasethi yoVavanyo lwe-antibody engathathi hlangothi

Kuvavanyo lomgangatho wesifo se-coronavirus ka-2019 (2019-nCOV okanye i-COVID -19) engathathi hlangothi i-antibody kwiserum yomntu/iplasma/igazi lilonke.

Ukusetyenziswa kobuchule kwi-Vitro Diagnostic Kuphela

【UKUSETYENZISWA OKUJOLISWEYO】

Ikhasethi yoVavanyo lwe-SARS-CoV-2 iNeutralizing antibody yichromatographic ekhawulezayo

I-immunoassay yokufunyaniswa komgangatho wokuthomalalisa i-antibody ye-Coronavirus Disease ka-2019 kwigazi elipheleleyo lomntu, i-serum, okanye i-plasma njengoncedo kumanqanaba oVavanyo lwe-coronavirus yabantu echasene ne-noveli i-antibody titer.

Izilwanyana ezanyisayo.I-genus γ ibangela ikakhulu usulelo lweentaka.I-CoV isasazwa ikakhulu ngokudibana ngokuthe ngqo nencindi okanye ngee-aerosols kunye namathontsi. Kukwakho nobungqina bokuba inokosulelwa ngendlela yelindle-ngomlomo.

I-Severe acute Respiratory Syndrome i-coronavirus 2 (SARS-CoV-2, okanye i-2019-nCoV) yintsholongwane ye-RNA egqunyiweyo engacandekiyo. Singunobangela wesifo se-coronavirus ka-2019 (COVID-19), esosulelayo ebantwini.

I-SARS-CoV-2 ineeproteni ezininzi zesakhiwo kubandakanya i-spike (S), imvulophu (E), i-membrane (M) kunye ne-nucleocapsid (N). Iprotheni ye-spike (S) iqulethe i-domain binding domain (RBD), ejongene nokuqaphela i-cell surface receptor, i-angiotensin eguqula i-enzyme-2 (ACE2). Kufunyaniswe ukuba i-RBD ye-SARS-CoV-2 S protein inxibelelana ngamandla ne-ACE2 receptor yomntu ekhokelela kwi-endocytosis kwiiseli zemiphunga enzulu kunye nokuphindaphindwa kwentsholongwane.

Ukosulelwa yi-SARS-CoV-2 iqala ukusabela komzimba, okubandakanya ukuveliswa kwee-antibodies egazini. Ii-antibodies ezifihliweyo zibonelela ngokukhusela kwizifo ezizayo ezivela kwiintsholongwane, kuba zihlala kwiinkqubo zokujikeleza kwegazi kwiinyanga ukuya kwiminyaka emva kokusuleleka kwaye ziya kubopha ngokukhawuleza kwaye ngamandla kwi-pathogen ukuvala ukungena kweselula kunye nokuphindaphinda. Ezi zikhuseli zibizwa ngokuba zii-neutheral antibodies.

【 UKUQOKELELWA KOMSEBENZI NOKULUNGISELELA 】

1.I-SARS-CoV-2 Ikhasethi yoVavanyo lwe-antibody ye-Neutralizing yenzelwe ukusetyenziswa negazi elipheleleyo lomntu, i-serum okanye isampuli zeplasma kuphela.

2.Imizekelo ecacileyo kuphela, engekho hemolyzed iyacetyiswa ukuba isetyenziswe ngolu vavanyo. I-Serum okanye i-plasma kufuneka ihlulwe ngokukhawuleza ukuphepha i-hemolysis.

3.Yenza uvavanyo ngoko nangoko emva kokuqokelela isampuli. Musa ukushiya iisampulu kwindawo yobushushu begumbi ixesha elide. Iisampuli zeSerum kunye neplasma zingagcinwa kwi-2-8 ° C ukuya kwiintsuku ezi-3. Ukugcinwa kwexesha elide, i-serum okanye iisampuli ze-plasma kufuneka zigcinwe ngaphantsi kwe-20 ° C. Igazi elipheleleyo eliqokelelwe yi-venipuncture kufuneka ligcinwe kwi-2-8 ° C ukuba uvavanyo luza kuqhutywa kwiintsuku ze-2 emva kokuqokelela. Musa ukumisa igazi elipheleleyo. imizekelo. Igazi elipheleleyo eliqokelelwe ngomnwe kufuneka livavanywe ngoko nangoko.

I-4.Iziqulatho eziqulethe i-anticoagulants ezifana ne-EDTA, i-citrate, okanye i-heparin kufuneka isetyenziswe ukugcinwa kwegazi elipheleleyo.Zisa iisampuli kwiqondo lokushisa ngaphambi kokuvavanya.

5.Imizekelo ekhenkcezisiweyo kufuneka inyibilikiswe ngokupheleleyo kwaye ixutywe kakuhle phambi kovavanyo.Kuphephe ukukhenkceza okuphindaphindiweyo

kunye nokunyibilika kwesampulu.

6.Ukuba iisampuli ziza kuthunyelwa, zipakishe ngokuhambelana nayo yonke imimiselo esebenzayo yokuthutha.

ye-etiological agents.

7.I-Icteric, i-lipemic, i-hemolyzed, i-heat treatment kunye ne-sera engcolisekileyo inokubangela iziphumo eziphosakeleyo.

8.Xa uqokelela igazi lentonga yomnwe nge-lancet kunye ne-alcohol pad, Nceda ulahle ithontsi lokuqala.

igazi elipheleleyo.

1. Zisa isingxobo kubushushu begumbi phambi kokusivula. Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.

2. Beka isixhobo sokuvavanya kwindawo ecocekileyo kunye nenqanaba.

NgeSerum okanye iSpecimens yePlasma: Ukusebenzisa i-Micropipette, kwaye udlulisele i-5ul serum / i-plasma kwi-specimen kakuhle yesixhobo sokuvavanya, uze udibanise i-2 drop of buffer, kwaye uqale isibali-xesha.

NgeGazi Elipheleleyo (iVenipuncture/Fingerstick) iisampuluGcoba umnwe wakho kwaye ucinezele ngobunono umnwe wakho, sebenzisa i-pipette yeplastiki elahlayo ukufunxa i-10ul yegazi elipheleleyo kumgca we-10ul wepayipi yeplastiki elahlwayo, kwaye uyigqithisele kumngxuma womzekelo wesixhobo sovavanyo (ukuba umthamo wegazi uphela udlula uphawu, Nceda ukhulule igazi elingaphezulu kwipipette), emva koko yongeza i-2 yethontsi yesithinteli, kwaye uqalise isibali-xesha. Qaphela: Imizekelo ingasetyenziswa kusetyenziswa i-micropipette.

3. Lindela umgca onemibala ukuba uvele.Funda iziphumo ngemizuzu eyi-15.Sukutolika iziphumo emva kwemizuzu engama-20.