I-SARS-cov-2 engathethekiyo i-Anti Kiking Kit (Elisa)
】Ukusetyenziswa kwenjongo】
I-SARS-Cov-2 Ukungathathi cala ikhithi ye-Anticyme i-Enzyme-i-Ignorment Assay I-Sars- Cov-2 Ukungathathi cala i-Antiction Tit kunokusetyenziswa njengoncedo ekuchongeni abantu abakwisabelo esifanelekileyo kwi-SARS- 2, ebonisa usulelo lwakutshanje okanye lwangaphambili. I-SARS-COV-2 Ukungathathi cala i-antidy kit engafunekiyo akufuneki isetyenziselwe ukufumanisa usulelo lwe-SARS-Cov-2.
】Intshayelelo】
Usulelo lwe-Coronavirus ngokwesiqhelo lubangelelanga iimpendulo ze-antid. Ireyithi ye-serocconvion kwizigulana ze-Covids-19 zingama-50% kunye ne-100% ngosuku lwesi-7 nele-14 kwi-OTTAMOT, ngokulandelelana. Ukubonisa ulwazi, intsholongwane ihambelana ne-antids engathathi cala kwigazi ivunywa njengethagethi yokusebenza kakuhle kunye nokusebenza okuphezulu kokungathathi cala ukuba ibonakalisa ukusebenza ngokukhuselekileyo. Uvavanyo lwe-plaque ukunciphisa ukuhlanjwa kwe-pluelids Nangona kunjalo, ngenxa yokuphuma kwayo ephantsi kunye nemfuno ephezulu yokusebenza, i-PRT ayisebenzi kumgangatho omkhulu we-serodiagnosis kunye novavanyo lonyango. I-SARS-2 engathethekiyo i-anticralting kitction i-Assoncedme-i-Inzzymedment Assoncesment Assay
】Inkqubo yovavanyo】
I-1in tubes ezahlukeneyo, i-alquot 120μl yesona sisombululo se-hace2-hrp.
I-2.ADD 6 μl yeCalibrators, iisampulu ezingaziwayo, ulawulo lomgangatho kwityhubhu nganye kwaye uxube kakuhle.
I-3.Transfer 800l yomxube ngamnye olungiselelwe inyathelo 2 kwi-microplate ehambelanayo ngokoqwalaselo lovavanyo olwenziweyo.
3.Cever iplate ngoTeteli ngeplate kwaye ubumbele kwi-37 ° C kwimizuzu engama-60.
4.Remove i-plate yokuthengisa iplate kwaye ihlambe ipleyiti nge-300 μm ye-1 μm ye-1 μ) ye-1 μ ye-1 × yolowa kwi-3
5. Iplate yeplate kwitawuli yephepha ukususa ulwelo lwentsalela kwiqula emva kokuhlamba.
6.Add 100 μl yesisombululo se-TMB nganye nganye kwaye ifake iplate ebumnyameni nge-20 ukuya kwi-25 ° C kangangemizuzu engama-20.
I-7.ADD 50 μl yesisombululo sokumisa kwi-pin nganye ukuba iyeke ukuphendula.
8. UKUHLAZIYWA KOMFANEKISO WOKUFUNDA UMFUNDI WOKUFUNDA NGE-450 NM ngaphakathi kwemizuzu eli-10 (i-630NM njengoko i-windows iyacetyiswa kwintsebenzo ephakamileyo yokuchaneka.
