I-SARS-CoV-2 Ikhithi yokuQongwa kwe-Antibody engathathi hlangothi (ELISA)
【UKUSETYENZISWA OKUHLOSIWEYO】
I-SARS-CoV-2 iNeutralizing Antibody Kit luKhuphiswano lwe-Enzyme-Linked Immunosorbent Assay (ELISA) ejoliswe ekubhaqweni okusemgangathweni kunye nesiqingatha sobuninzi be-anti-anti-neutralizing antibodies kwi-SARS-CoV-2 kwiserum yomntu kunye neplasma. I-SARS-CoV-2 Neutralizing Antibody Detection Kit inokusetyenziswa njengoncedo ekuchongeni abantu abanempendulo yokuzikhusela kwi-SARS-CoV-2, ebonisa usulelo lwamva nje okanye lwangaphambili. I-SARS-CoV-2 Neutralizing Antibody Detection Kit akufuneki isetyenziswe ukufumanisa usulelo oluqatha lwe-SARS-CoV-2.
【INTSHAYELELO】
Usulelo lweCoronavirus ludla ngokubangela ukuba umntu angakwazi ukuphendula. Amazinga e-seroconversion kwizigulana ze-COVID-19 ngama-50% kunye ne-100% ngosuku lwesi-7 kunye ne-14 emva kweempawu zokuqala, ngokulandelelanayo. Ukubonisa ulwazi, intsholongwane ehambelanayo ne-antibody ehambelanayo egazini ibonwa njengento ekujoliswe kuyo ekumiseleni ukusebenza kakuhle kwe-antibody kunye noxinaniso oluphezulu lwe-antibody engathathi hlangothi lubonisa ukusebenza kakuhle kokhuseleko. Uvavanyo lwe-Plaque Reduction Neutralization (PRNT) luye lwaqatshelwa njengomgangatho wegolide wokubona izilwa-buhlungu ezingathathi hlangothi. Nangona kunjalo, ngenxa yomthamo ophantsi kunye nemfuneko ephezulu yokusebenza, i-PRNT ayisebenzi kumlinganiselo omkhulu we-serodiagnosis kunye novavanyo lwesitofu. I-SARS-CoV-2 ye-Neutralizing Antibody Detection Kit isekwe kuKhuphiswano lwe-Enzyme-Linked Immunosorbent Assay (ELISA) indlela yokubona i-antibody engathathi hlangothi kwisampulu yegazi kunye nokufikelela ngokukodwa kumanqanaba oxinaniso olu hlobo lwe-antibody.
【Inkqubo yoVavanyo】
1.Kwiibhubhu ezahlukeneyo, i-aliquot 120μL yesisombululo esilungisiweyo se-hACE2-HRP.
2.Yongeza i-6 μL ye-calibrators, iisampuli ezingaziwayo, ulawulo lwekhwalithi kwi-tube nganye kwaye udibanise kakuhle.
3.Ukutshintshela i-100μL yomxube ngamnye olungiselelwe kwinqanaba lesi-2 ukuya kumaqula e-microplate ahambelanayo ngokucwangciswa kwangaphambili kovavanyo.
3.Ggquma ipleyiti ngePlate Sealer uze uyifukamele kwi-37°C imizuzu engama-60.
4. Susa isitywina sePlate kwaye uhlambe ipleyiti malunga ne-300 μL ye-1× Isisombululo sokuhlanjwa kwequla ngalinye amaxesha amane.
5.Cofa ipleyiti kwitawuli yephepha ukususa ulwelo olushiyekileyo equleni emva kwamanyathelo okuhlamba.
6.Yongeza i-100 μL ye-TMB Isisombululo kwiqula ngalinye kwaye ufukamele ipleyiti ebumnyameni kwi-20 - 25 ° C imizuzu engama-20.
7.Yongeza i-50 μL yeStop Solution kwiqula ngalinye ukumisa ukusabela.
8.Funda i-absorbence kwi-microplate reader kwi-450 nm ngaphakathi kwemizuzu eyi-10 (i-630nm njenge-accessory inconywa ukuba isebenze ngokuchaneka okuphezulu.
