Inyathelo elinye loVavanyo lwe-SARS-CoV2(COVID-19)IgG/IgM
Ukusetyenziswa okucetywayo
Uvavanyo lwe-One Step SARS-CoV2(COVID-19)IgG/IgM luvavanyo olukhawulezayo lwe-chromatographic immunoassay ukuze kufunyanwe umgangatho we-antibodies (IgG kunye ne-IgM) ukuya kwintsholongwane ye-COVID-19 kwiGazi Elipheleleyo / iSerum / iPlasma ukunceda ekufunyanisweni kwe-COVID. -19 usulelo lwentsholongwane.
Isishwankathelo
Iintsholongwane zeCorona ziintsholongwane ze-RNA ezigutyungelweyo ezisasazwa ngokubanzi phakathi kwabantu, ezinye izilwanyana ezanyisayo, kunye neentaka kwaye ezibangela izifo zokuphefumla, ze-enteric, i-hepatic kunye ne-neurologic. Iintlobo ezisixhenxe zentsholongwane ye-corona zaziwa ngokubangela izifo zabantu. Iintsholongwane ezine-229E. OC43. I-NL63 kunye ne-HKu1- zixhaphakile kwaye zidla ngokubangela iimpawu ezibandayo ezixhaphakileyo kubantu abangenamandla omzimba.4 Ezinye iintlobo ezintathu-ezinzima kakhulu zokuphefumla isifo se-coronavirus (SARS-Cov), Middle East breathing syndrome coronavirus (MERS-Cov) kunye neNoveli yeCoronavirus (COVID-2019) 19)- ziyi-zoonotic imvelaphi kwaye ziye zanxulunyaniswa nesigulo esibulalayo ngamanye amaxesha. Izilwa-buhlungu ze-IgG kunye ne-lgM ukuya ku-2019 iNoveli yeCoronavirus inokufunyanwa kwiiveki ezi-2 ukuya kwezi-3 emva kokuba sesichengeni. I-lgG ihlala ilungile, kodwa inqanaba le-antibody liyehla ixesha elongezelelweyo.
Umgaqo
I-One Step SARS-CoV2(COVID-19)IgG/IgM (Igazi Elipheleleyo/iSerum/Plasma) luvavanyo olusecaleni lokuhamba kwe-immunochromatographic. Uvavanyo lusebenzisa i-anti-human LgM antibody (umgca wovavanyo we-IgM), i-lgG echasene nomntu (umgca wovavanyo we-lgG kunye nebhokhwe yokuchasa imivundla igG (umgca wolawulo C) engashukumiyo kumcu we-nitrocellulose. Ii-antigens ze-COVID-19 ezidityaniswe negolide ye-colloid (COVID-19 I-conjugatesand nomvundla we-lgG-gold conjugates Xa umfuziselo olandelwa sisithinteli sovavanyo songezwa kwisampulu kakuhle, izilwa-buhlungu ze-IgM &/okanye ze-lgG ukuba zikhona, ziya kubophelela kwi-COVID-19 conjugates eyenza i-antigen antibodies ifuduke ngenwebu ye-nitrocellulose intshukumo &/okanye i-anit-human lgG) i-complex ivaleleke yenza i-burgundy band enemibala eqinisekisa isiphumo sovavanyo olusebenzayo kwingingqi yovavanyo lubonisa iziphumo zovavanyo ezingasebenziyo.
Uvavanyo iqulathe ulawulo lwangaphakathi (C band) ekufuneka abonise band burgundy enemibala ye immunocomplex ibhokhwe anti umvundla IgG/umvundla lgG-gold conjugate kungakhathaliseki ukuba uphuhliso umbala nakweyiphi na bands uvavanyo. Kungenjalo, iziphumo zovavanyo azisebenzi kwaye umboniso kufuneka uphinde uvavanywe ngesinye isixhobo.
Ukugcinwa kunye nokuzinza
- Gcina njengoko zipakishwe kwisingxobo esitywiniweyo kwiqondo lobushushu begumbi okanye efrijini (4-30℃ okanye 40-86℉). Isixhobo sovavanyo sizinzile ngomhla wokuphelelwa oshicilelweyo kwisingxobo esivaliweyo.
- Uvavanyo maluhlale kwisingxobo esivaliweyo de lusetyenziswe.
Izixhobo eziZodwa ezongezelelweyo
Izixhobo ezibonelelweyo:
| .Izixhobo zovavanyo | . Iidropha zomzekelo ezilahlwayo |
| . Isithinteli | . Faka iphakheji |
Izinto ezifunekayo kodwa azibonelelwanga:
| . Centrifuge | . Isibali-xesha |
| . Ipadi yotywala | . Izikhongozeli zokuqokelela imizekelo |
Ukulumkela
☆ Ukusetyenziswa koxilongo lwe-in vitro kuphela. Musa ukusebenzisa emva komhla wokuphelelwa.
☆ Sukutya, usele okanye utshaye kwindawo apho imizekelo kunye neekiti ziphathwa.
☆ Phatha yonke imizekelo ngokungathi inee-arhente ezosulelayo.
☆ Jonga izilumkiso ezimiselweyo malunga nobungozi bebhayoloji kuzo zonke iinkqubo kwaye ulandele iinkqubo ezisemgangathweni zokulahlwa ngokufanelekileyo kwesampulu.
☆ Nxiba iimpahla ezikhuselayo ezifana needyasi zaselabhoratri, iiglavu ezilahlwayo kunye nokhuseleko lwamehlo xa imizekelo ivavanywa.
☆ Landela izikhokelo ezisemgangathweni zokhuseleko lwebhayoloji yokuphatha kunye nokulahlwa kwemathiriyeli enokubakho eyosulelayo.
☆ Ukufuma kunye nobushushu bunokuchaphazela kakubi iziphumo.
Ukuqokelelwa koMfanekiso kunye noLungiselelo
1. UVavanyo lwe-SARS-CoV2(COVID-19)IgG/IgM lunokwenziwa kwiGazi Elipheleleyo/iSerum/Plasma.
2. Ukuqokelela igazi elipheleleyo, i-serum okanye i-plasma isampuli ezilandela iinkqubo eziqhelekileyo zelebhu yekliniki.
3. Uvavanyo kufuneka lwenziwe ngokukhawuleza emva kokuqokelelwa kwesampuli. Musa ukushiya imizekelo kwiqondo lobushushu begumbi ixesha elide. Ukugcina ixesha elide, iisampuli kufuneka zigcinwe ngaphantsi -20 ℃. Igazi elipheleleyo kufuneka ligcinwe ku-2-8℃ ukuba uvavanyo luza kwenziwa kwiintsuku ezi-2 zokuqokelelwa. Musa ukukhenkcisa iisampuli zegazi elipheleleyo.
4. Zisa iisampulu kwiqondo lobushushu legumbi ngaphambi kovavanyo. Iisampulu ezikhenkcezisiweyo kufuneka zinyibilike ngokupheleleyo kwaye zixutywe kakuhle phambi kovavanyo. Iisampulu akufuneki zifakwe emkhenkceni kwaye zinyibilike ngokuphindaphindiweyo.
Inkqubo yoVavanyo
1. Vumela uvavanyo, isampuli, isithinteli kunye/okanye ulawulo ukufikelela kwiqondo lobushushu begumbi 15-30℃ (59-86℉) phambi kovavanyo.
2. Zisa isingxobo kwiqondo lobushushu begumbi phambi kokuba usivule. Susa isixhobo sovavanyo kwisingxobo esivaliweyo kwaye usisebenzise ngokukhawuleza.
3. Beka isixhobo sokuvavanya kwindawo ecocekileyo kunye nenqanaba.
4. Bamba i-dropper ngokuthe nkqo kwaye udlulisele i-1 drop ye-specimen (malunga ne-10μl) kwi-specimen well(S) yesixhobo sovavanyo, uze udibanise ama-2 amathontsi e-buffer (malunga ne-70μl) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.
5. Lindela ukuba kuvele umgca onemibala. Funda iziphumo ngemizuzu eyi-15. Musa ukutolika umphumo emva kwemizuzu engama-20.
Amanqaku:
Ukusebenzisa isixa esaneleyo somzekelo kubalulekile kwisiphumo sovavanyo esisebenzayo. Ukuba ukufuduka (ukumanzisa inwebu) akujongwa kwifestile yovavanyo emva komzuzu omnye, yongeza ithontsi elinye le-buffer kumzekelo kakuhle.
Ukutolikwa kweziPhumo
Okuhle:Umgca wokulawula kwaye ubuncinane umgca wokuvavanya uvela kwi-membrane. Inkangeleko yomgca wovavanyo we-T2 ibonisa ubukho bee-anti-IgG ezithile ze-COVID-19. Inkangeleko yomgca wovavanyo we-T1 ibonisa ubukho be-COVID-19 ethile yokulwa izilwa-buhlungu ze-IgM. Kwaye ukuba zombini umgca we-T1 kunye ne-T2 uyavela, oko kubonisa ukuba ubukho bazo zombini izilwa-buhlungu ze-IgG kunye ne-IgM ze-COVID-19. Okukhona kusezantsi ugxininiso lwe-antibody, kokukhona buthathaka umgca wesiphumo.
Engalunganga:Umgca omnye onemibala ubonakala kummandla wokulawula (C) .Akukho mgca wembala obonakalayo ovela kummandla wovavanyo.
Ayisebenzi:Umgca wolawulo uyasilela ukubonakala. Umthamo wesampulu engonelanga okanye ubuchule benkqubo obungachanekanga zezona zizathu ezinokuthi zibe kho zokungaphumeleli komgca wolawulo. Phonononga inkqubo kwaye uluphinde uvavanyo ngesixhobo esitsha sovavanyo. Ukuba ingxaki iyaqhubeka, yeka ukusebenzisa ikiti yovavanyo ngoko nangoko kwaye uqhagamshelane nomsasazi wakho wendawo.
Ukulinganiselwa
1.Uvavanyo lwe-SARS-CoV2(COVID-19)IgG/IgM lolwe-in vitro diagnostic kuphela. Uvavanyo kufuneka lusetyenziselwe ukufunyaniswa kwezilwa-buhlungu ze-COVID-19 kwiGazi Elipheleleyo / iSerum / iisampulu zePlasma kuphela. Ixabiso lobungakanani okanye izinga lokunyuka kwe-2. Izilwa-buhlungu ze-COVID-19 zinokumiselwa ngolu vavanyo lomgangatho.
3. Njengazo zonke iimvavanyo zokuxilonga, zonke iziphumo kufuneka zitolikwe kunye nolunye ulwazi lwekliniki olukhoyo kugqirha.
4. Ukuba iziphumo zovavanyo zibi kwaye iimpawu zeklinikhi ziqhubeka, uvavanyo olongezelelweyo usebenzisa ezinye iindlela zeklinikhi ziyacetyiswa. Isiphumo esibi asikuthinteli nangaliphi na ixesha ukuba nokwenzeka kosulelo lwentsholongwane ye-COVID-19.
Ulwazi Lomboniso
Isatifikethi sembeko
Iprofayile yekhampani
Thina, i-Hangzhou Testsea Biotechnology Co., Ltd yinkampani ekhula ngokukhawuleza yobuchwephesha bebhayoloji ekhethekileyo ekuphandeni, ekuphuhliseni, ekuveliseni nasekusasazeni iikhithi zovavanyo lwe-in-vitro diagnostic (IVD) kunye nezixhobo zonyango.
Indawo yethu yi-GMP, ISO9001, kunye ne-ISO13458 eqinisekisiweyo kwaye sinemvume ye-CE FDA. Ngoku sijonge phambili ekusebenzisaneni neenkampani zaphesheya kwezilwandle ngophuhliso olufanayo.
Sivelisa uvavanyo lokuchuma, uvavanyo lwezifo ezosulelayo, uvavanyo lokusetyenziswa kakubi kweziyobisi, uvavanyo lwentliziyo, uvavanyo lokumakisha ithumba, uvavanyo lokutya kunye nokhuseleko kunye novavanyo lwezifo zezilwanyana, ukongezelela, uphawu lwethu lwe-TESTSEALABS luye lwaziwa kakhulu kwiimarike zasekhaya naphesheya. Umgangatho ogqwesileyo kunye namaxabiso ancomekayo asenza sikwazi ukuthatha ngaphezulu kwe-50% yezabelo zasekhaya.
Inkqubo yeMveliso
1.Lungiselela
2.Ikhava
3.Inwebu enqamlezileyo
4.Sika umcu
5.Indibano
6.Pakisha iipowutshi
7.Zitywine iingxowa
8.Pakisha ibhokisi
9.Encasement
