Umvelisi we-OEM waseTshayina iMedomics eyosulelayo e-COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist

Ngoku sinabasebenzisi abaninzi abagqwesileyo kwintengiso ye-intanethi, i-QC, kunye nokusebenza kunye neentlobo zengxaki enzima ngaphakathi kwendlela yokuvelisa ye-OEM Manufacturer China Medomics I-Infectious COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist, siye saba sinye. yabavelisi bakho abakhulu be-100% eTshayina.Uninzi lwamashishini amakhulu okurhweba angenisa iimveliso kunye nezisombululo kuthi, ngoko sinokukunika ngokulula eyona thegi yexabiso enenzuzo efanayo kuye nabani na onomdla kuthi.
Ngoku sinabasebenzisi abaninzi abagqwesileyo babasebenzi abagqwesileyo kwintengiso ye-intanethi, i-QC, kwaye sisebenza neentlobo zengxaki enzima ngaphakathi kwendlela yokuvelisaUmzi-mveliso we-Antibody Neutralizing, I-Antibody Neutralizing umenzi, Antibody Neutralizing umboneleli, Uvavanyo lwe-Antibody Neutralizing, China Medical, I-Antibody engathathi hlangothi, Nokuba ukhetha imveliso yangoku kwikhathalogu yethu okanye ufuna uncedo lobunjineli kwisicelo sakho, unokuthetha neziko lethu lenkonzo yabathengi malunga neemfuno zakho zokukhangela.Sinokubonelela ngekhwalithi elungileyo ngexabiso elikhuphisanayo kuwe buqu.

【UMGAQO】

I-SARS-CoV-2I-Antibody engathathi hlangothiI-Detection Kit isekwe kwindlela ye-ELISA yokhuphiswano.

Ukusebenzisa i-domain binding ehlanjululweyo ye-receptor (RBD), iprotheni evela kwi-viral spike (S) protein kunye ne-host cell

receptor ACE2, olu vavanyo lwenzelwe ukulinganisa i-virus-host neutralizing intsebenziswano.

I-Calibrators, uLawulo loMgangatho, kunye ne-serum okanye iisampulu zeplasma zixutywe kakuhle kwi-dilution

isithinteli esiqulethe i-hACE2-HRP conjugate aliquoted kwiityhubhu ezincinci.Emva koko imixube idluliselwa ngaphakathi

imingxuma yemicroplate equlathe immobilized recombinant SARS-CoV-2 RBD fragment (RBD) for

ekufukameni.Ngexesha le-30-minute incubation, i-antibody ye-RBD ekhethekileyo kwii-calibrators, i-QC kunye

iisampuli ziya kukhuphisana kunye ne-hACE2-HRP ngokubopha ngokuthe ngqo i-RBD engenakunyakaziswa emigodini.Emva koko

i-incubation, imingxuma ihlanjwe amaxesha angama-4 ukususa i-conjugate ye-hACE2-HRP engabotshwanga.Isisombululo se

I-TMB iye yongezwa kwaye ifukanywe imizuzu engama-20 kwiqondo lobushushu begumbi, okukhokelela kuphuhliso lwe-a

umbala oluhlaza.Ukuphuhliswa kombala kumisiwe kunye nokongezwa kwe-1N HCl, kunye ne-absorbence

kulinganiswe i-spectrophotometrically kwi-450 nm.Ubunzulu bombala owenziweyo buhambelana ne

Ubungakanani be-enzyme ekhoyo, kwaye inxulumene ngokungafaniyo nobungakanani bemigangatho evavanyiweyo ngendlela efanayo.

Ngokuthelekisa kunye negophe lokulinganisa elenziwe ngabalinganisi ababonelelweyo, ukugxila kwe

amajoni omzimba neutralizing kwisampulu engaziwayo ke ibalwe.

【IZINTO EZIFUNEKAYO KODWA AKUNIKEZWA】

1. Amanzi adibeneyo okanye ahlanjululweyo

2. Iipayipi ezichanekileyo: 10μL, 100μL, 200μL kunye ne-1 ml

3. Iingcebiso zepipette ezilahlayo

4. Umfundi weMicroplate okwaziyo ukufunda i-absorbence kwi-450nm.

5. Iphepha lokufunxa

6. Iphepha legrafu

7. Umxube weVortex okanye olinganayo

【UKUQOKELELWA KOMSEBENZI NOKUGCINWA】

1. Iisampulu zeSerum kunye nePlasma eziqokelelwe kwiityhubhu eziqulethe i-K2-EDTA zingasetyenziselwa le khithi.

2. Imizekelo kufuneka ifakwe kwi-cap kwaye inokugcinwa ukuya kutsho kwiiyure ezingama-48 kwi-2 °C - 8 °C phambi kovavanyo.

Iisampulu ezigcinwe ixesha elide (ukuya kwiinyanga ezi-6) kufuneka zikhenkcezwe kube kanye kuphela kwi -20 °C phambi kovavanyo.

Kuphephe ukuphindaphinda imijikelo yokunyibilika komkhenkce.

IPROTOCOL

【Ukulungiswa kweReagent】

1. Zonke ii-reagents kufuneka zikhutshwe kwifriji kwaye zivunyelwe ukuba zibuyele kwiqondo lokushisa ngaphambi kokusetyenziswa

(20° ukuya kuma-25°C).Gcina zonke ii-reagents kwifriji ngokukhawuleza emva kokusetyenziswa.

2. Zonke iisampulu kunye nolawulo kufuneka vortexed phambi kokusetyenziswa.

3. I-hACE2-HRP Ukulungiswa kwesisombululo: Dilute i-hACE2-HRP igxininise ku-1: 51 umlinganiselo we-dilution kunye ne-Dilution

Isithinteli.Umzekelo, dilute i-100 μL ye-hACE2-HRP yoxinaniso nge-5.0mL ye-HRP Dilution Buffer ukuya

yenza isisombululo se-hACE2-HRP.

4. 1× Ukuhlamba Isisombululo Ukulungiselela: Nciphisa i-20× yokuhlamba Isisombululo ngamanzi adityanisiweyo okanye adibeneyo

umlinganiselo womthamo we-1:19.Umzekelo, dilute 20 mL of 20× Hlamba Isisombululo nge 380 mL ye deionized okanye

amanzi adibeneyo ukwenza i-400 mL ye-1 × Isisombululo sokuhlamba.

【Inkqubo yoVavanyo】

1. Kwimibhobho eyahlukileyo, i-aliquot 120μL yesisombululo esilungisiweyo se-hACE2-HRP.

2. Yongeza i-6 μL ye-calibrators, iisampuli ezingaziwayo, ulawulo lwekhwalithi kwi-tube nganye kwaye udibanise kakuhle.

3. Dlulisa i-100μL yomxube ngamnye olungiselelwe kwinqanaba lesi-2 kumaqula e-microplate ahambelanayo.

kuqwalaselo lovavanyo oluyilwe kwangaphambili.

3. Gquma ipleyiti ngePlate Sealer uze uyifukamele kwi-37°C imizuzu engama-30.

4. Susa iSitywini sePlayiti uze uhlambe ipleyiti malunga ne-300 μL ye-1× Isicombululo sokuHlamba kwiqula ngalinye amaxesha amane.

5. Cofa ipleyiti kwitawuli yephepha ukususa ulwelo olushiyekileyo equleni emva kokuhlamba amanyathelo.

6. Yongeza i-100 μL ye-TMB Isisombululo kwiqula ngalinye kwaye ufukamele ipleyiti ebumnyameni kwi-20 - 25 ° C imizuzu engama-20.

7. Yongeza i-50 μL yeStop Solution kwiqula ngalinye ukumisa ukusabela.

8. Funda i-absorbence kwi-microplate reader kwi-450 nm ngaphakathi kwemizuzu eyi-10 (630nm njenge-accessory).

kucetyiswa ukusebenza ngokuchanekileyo okuphezulu).