I-SARS-CoV-2 Ikhithi yokuFumana ye-RT-PCR yexesha lokwenyani

Le khithi yenzelwe ukufunyaniswa komgangatho we-in vitro we-ORF1ab kunye nofuzo lwe-N ukusuka ku-2019-nCoV kwi-pharyngeal swab okanye i-bronchoalveolar lavage isampulu eziqokelelwe kwisifo se-Coronavirus ka-2019 (COVID-19) amatyala arhanelekayo, amaqela akrokrelekayo amatyala, okanye abanye abantu abafuna u-2019 - Ukuxilongwa kosulelo lwe-nCoV okanye ukuxilongwa kokwahlula.

Ikhithi yenzelwe ukufunyanwa kwe-RNA ye-2019-nCoV kwimizekelo esebenzisa iteknoloji ye-multiplex yexesha langempela le-RTPCR kunye nemimandla egciniweyo ye-ORF1ab kunye nofuzo lwe-N njengeendawo ezijoliswe kuzo ze-primers kunye ne-probes. Ngaxeshanye, le kit iqulethe inkqubo yokufumanisa ulawulo olungapheliyo (I-gene yokulawula ibhalwe nguCy5) ukubeka iliso kwinkqubo yokuqokelela i-specimen, i-nucleic acid extraction kunye ne-PCR kunye nokunciphisa iziphumo ezingalunganga.

Ezona mpawu:

1. Ukwandiswa okuKhawulezayo, okuthembekileyo kunye nokubandakanywa kokufumanisa: i-SARS njenge-coronavirus kunye nokuchongwa okuthe ngqo kwe-SARS-CoV-2

2. Inyathelo elinye le-RT-PCR reagent (i-lyophilized powder)

3. Ibandakanya ulawulo olulungileyo nolungalunganga

4. Ukuthutha kwiqondo lokushisa eliqhelekileyo

5. Ikhithi ingahlala izinzile ukuya kutsho kwiinyanga ezili-18 ezigcinwe ku -20℃.

6. CE ivunyiwe

Ukuhamba :

1. Lungiselela i-RNA ekhutshwe kwi-SARS-CoV-2

2. Nciphisa ulawulo olulungileyo lwe-RNA ngamanzi

3. Lungisa i-PCR master mix

4. Faka isicelo se-PCR master mix kunye ne-RNA kwi-real-time PCR plate okanye ityhubhu

5. Qhuba isixhobo sePCR yexesha langempela