I-Immunology sisifundo esintsonkothileyo esinolwazi oluninzi lobuchwephesha.Eli nqaku lijonge ukwazisa ukuba iimveliso zethu zisebenzise ulwimi olufutshane oluqondakalayo.
Kwintsimi yokufumanisa ngokukhawuleza, ukusetyenziswa kwekhaya ngokuqhelekileyo kusebenzisa indlela yegolide ye-colloidal.
Iinanoparticles zegolide zidityaniswa ngokulula kwizilwa-buhlungu, iipeptides, iioligonucleotides zokwenziwa, kunye nezinye iiproteni ngenxa yokudibana kwamaqela e-sulfhydryl (-SH) kumphezulu wegolide.3-5.Iiconjugates zegolide-biomolecule zibandakanywe ngokubanzi kwizicelo zokuxilonga, apho umbala wazo obomvu oqaqambileyo usetyenziswa ekhaya kunye novavanyo lokukhathalela olufana novavanyo lokukhulelwa ekhaya.
Ngenxa yokuba umsebenzi ulula, umphumo kulula ukuyiqonda, kulula, ngokukhawuleza, kuchanekile kunye nezinye izizathu.Indlela yegolide yeColloidal yeyona ndlela iphambili yokufumanisa ngokukhawuleza kwimarike.
Ukhuphiswano kunye novavanyo lwesangweji yimifuziselo ephambili ye-2 kwindlela yegolide ye-colloidal, Baye batsala umdla ngenxa yeefomathi zabo ezinobubele zabasebenzisi, amaxesha amafutshane okuvavanya, ukuphazamiseka okuncinci, iindleko eziphantsi, kunye nokuba lula ngokuqhutywa ngabasebenzi abangabodwa.Obu buchule busekelwe kwintsebenziswano ye-biochemical ye-antigen-antibody hybridization.Iimveliso zethu zenziwe ngamacandelo amane: iphedi yesampula, eyona ndawo apho isampuli ilahleka khona;i-conjugate pad, apho iithegi ezibhaliweyo zidityaniswe nezinto zokuqaphela izinto eziphilayo;i-reaction membrane equlethe umgca wovavanyo kunye nomgca wokulawula ukusebenzisana kwe-antigen-antibody;kunye nephedi yokufunxa, egcina inkunkuma.
1.Umgaqo-siseko woVavanyo
Izilwa-buhlungu ezimbini ezibophelela iiepitopes ezahlukileyo ezikhoyo kwimolekyuli yentsholongwane ziyasetyenziswa.Enye (i-antibody yokugquma) ebhalwe nge-nanoparticles yegolide e-colloidal kunye nenye (i-antibody yokubamba) ebekwe kumphezulu we-NC inwebu.I-antibody yokugquma ikwimeko yokuphelelwa ngamanzi emzimbeni ngaphakathi kwephedi ye-conjugate.Xa isisombululo esisemgangathweni okanye isampulu zongezwa kwisampulu yephedi yovavanyo, isibophelelo sinokunyibilika ngoko nangoko xa sidibana nesixhobo esinamanzi esinentsholongwane.Emva koko i-antibody yenza i-complex enentsholongwane kwisigaba solwelo kwaye yaqhubela phambili ngokuqhubekayo de yabanjwa yi-antibody egxininiswe kumphezulu we-NC membrane, eyenza umqondiso ngokulinganayo malunga noxinzelelo lwentsholongwane.Ngaphaya koko, i-antibody eyongezelelweyo ethile kwi-antibody yokugquma ingasetyenziselwa ukuvelisa uphawu lolawulo.Iphedi yokufunxa ibekwe phezulu ukuze ibangele yi-capillarity eyenza ukuba i-immune complex itsalelwe kwi-antibody esisigxina.Umbala obonakalayo uvele ngaphantsi kwemizuzu eyi-10, kwaye ubuninzi bunquma inani lentsholongwane.Ngamanye amazwi, intsholongwane eninzi ekhoyo kwisampuli, ibonakala ngakumbi ibhendi ebomvu.
Makhe ndichaze ngokufutshane ukuba ezi ndlela zimbini zisebenza njani:
1.Double anti sandwich indlela
Umgaqo-nkqubo wendlela ye-anti sandwich ephindwe kabini, esetyenziswa ikakhulu ukuchongwa kweprotheyini yobunzima bemolekyuli (anti).
2. Indlela yokhuphiswano
Indlela yokhuphiswano ibhekisela kwindlela yokufumanisa i-antigen ehlanganiswe ngumgca wokufumanisa kunye ne-antibody yophawu lwegolide ye-antigen ukuba ihlolwe.Iziphumo zale ndlela zifundwa ngokuchasene neziphumo zendlela yesandwich, kunye nenye. umgca kwi-positive kunye nemigca emibini kwelilathisi.
Ixesha lokuposa: Dec-03-2019