I-Monkeypox Virus (MPV) i-Nucleic Acid Detection Kit
INTSHAYELELO
Ikhithi isetyenziselwa uchongo lwe-in vitro lomgangatho weziganeko ezikrokrelekayo zeMonkeypox Virus (MPV), iimeko ezidityanisiweyo kunye nezinye iimeko ezifuna ukufunyaniswa ukuba zinosulelo lweMonkeypox Virus.
Ikhithi isetyenziselwa ukukhangela ijini ye-f3L yeMPV kwi-swabs yomqala kunye neesampuli zempumlo.
Iziphumo zovavanyo zale khithi zezereferensi yeklinikhi kuphela kwaye akufanele zisetyenziswe njengeyona ndlela yodwa yokuxilongwa kwekliniki. Kunconywa ukwenza uhlalutyo olubanzi lwemeko esekelwe kwiklinikhi yesigulane
ukubonakaliswa kunye nolunye uvavanyo lwebhubhoratri.
Ukusetyenziswa okucetywayo
| Uhlobo lwesivavanyi | umqala kunye ne-swab yeempumlo |
| Uhlobo lovavanyo | Umgangatho |
| Izinto zovavanyo | I-PCR |
| Ubungakanani bepakethi | Iimvavanyo ezingama-48/1 ibhokisi |
| Ubushushu bokugcina | 2-30℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-10 |
UMBONO WEMVELISO
Umgaqo
Le khithi ithatha ulandelelwano oluthile olugciniweyo lwe-MPV f3L gene njengommandla ekujoliswe kuwo. Itekhnoloji yexesha langempela le-fluorescence quantitative PCR kunye ne-nucleic acid yokukhululwa ngokukhawuleza itekhnoloji isetyenziselwa ukubeka esweni i-viral nucleic acid ngokutshintshwa kwesignali ye-fluorescence yeemveliso zokukhulisa. Inkqubo yokufumanisa ibandakanya ukulawulwa kwekhwalithi yangaphakathi, esetyenziselwa ukubeka iliso ukuba ngaba kukho i-PCR inhibitors kwiisampuli okanye ukuba iiseli kwiisampuli zithathwa, ezinokuthi zithintele ngokufanelekileyo imeko embi yobuxoki.
AMANQAKU APHAMBILI
Ikhithi iqulethe ii-reagents zokuqhuba iimvavanyo ezingama-48 okanye ulawulo lomgangatho, kubandakanya la macandelo alandelayo:
I-Reagent A
| Igama | Amacandelo aphambili | Ubungakanani |
| Ukufunyanwa kweMPV ireagent | Ityhubhu yokusabela iqulethe iMg2+, f3L gene / Rnase P primer probe, isithinteli reaction, Taq DNA enzyme. | Iimvavanyo ezingama-48 |
I-ReagentB
| Igama | Amacandelo aphambili | Ubungakanani |
| MPV Ulawulo Olulungileyo | Iqulathe iqhekeza lenjongo yeMPV | ityhubhu e-1 |
| MPV Ulawulo olubi | Ngaphandle kweqhekeza lenjongo yeMPV | ityhubhu e-1 |
| Ukukhutshwa kwe-reagent ye-DNA | I-reagent iqulethe i-Tris, i-EDTA kunye neTriton. | 48pcs |
| I-reagent yohlengahlengiso | DEPC anyangwe amanzi | 5ML |
Qaphela: Amalungu amanani eebhetshi ahlukeneyo akanakusetyenziswa ngokutshintshanayo
【Iimeko zoGcino kunye noBomi beShelf】
I-1.Reagent A / B inokugcinwa kwi-2-30 ° C, kwaye ubomi beshelufu ziinyanga ezili-10.
2.Nceda uvule ikhava yetyhubhu yovavanyo kuphela xa ulungele uvavanyo.
3.Musa ukusebenzisa iityhubhu zovavanyo ngaphaya komhla wokuphelelwa.
4.Musa ukusebenzisa ityhubhu yokubona evuzayo.
【Isixhobo Esisebenzayo】
Ifanelekile kwi-LC480 PCR inkqubo yokuhlalutya, i-Gentier 48E Automatic PCR system analysis, ABI7500 PCR system analysis.
【IiMfuno zeSampulu】
1.Iintlobo zeesampuli ezisebenzayo: iisampulu zomqala.
2.Isisombululo sesampulu:Emva kokuqinisekiswa, kuyacetyiswa ukuba kusetyenziswe i-saline eqhelekileyo okanye ityhubhu yogcino lweNtsholongwane eveliswe yi-Hangzhou Testsea biology ukuqokelela isampulu.
umqala:sula iitoni ze-pharyngeal zamazwe amabini kunye nodonga lwangasemva lwe-pharyngeal kunye ne-sposal sterile sampling swab, cwilisa i-swab kwi-tube equlethe isisombululo se-sampling ye-3mL, ulahle umsila, kwaye uqinise isigqubuthelo sombhobho.
3.Isampuli yokugcina kunye nokuhanjiswa:Iisampulu eziza kuvavanywa kufuneka zivavanywe ngokukhawuleza. Ubushushu bokuthutha kufuneka bugcinwe kwi-2 ~ 8 ℃. Iisampuli ezinokuvavanywa kwiiyure ezingama-24 zingagcinwa kwi-2 ℃ ~ 8 ℃ kwaye ukuba iisampuli azikwazi ukuvavanywa kwiiyure ezingama-24, kufuneka zigcinwe ngaphantsi okanye zilingana. ukuya ku -70 ℃ (ukuba akukho meko yokugcina -70 ℃, inokugcinwa ku -20 ℃ okwethutyana), kunqande ukuphinda
ukukhenkceza nokunyibilika.
I-4.Ukuqokelela isampula efanelekileyo, ukugcinwa, kunye nokuthutha kubaluleke kakhulu ekusebenzeni kwale mveliso.
【Indlela yoVavanyo】
1.Ukwenziwa kwesampuli kunye nokudibanisa isampuli
1.1 Ukwenziwa kwesampuli
Emva kokuxuba isisombululo sesampulu esingentla kunye neesampulu, thatha i-30μL yesampuli kwi-DNA yokukhupha i-reagent tube kwaye uyixube ngokulinganayo.
1.2 Iyalayisha
Thatha i-20μL ye-reagent yokubuyisela kwaye uyifake kwi-reagent yokufumanisa i-MPV, yongeza i-5μL yesampuli eqhutywe ngasentla (Ulawulo olulungileyo kunye nolawulo olubi luya kucutshungulwa ngokuhambelana neesampuli), vala i-tube cap, i-centrifuge kwi-2000rpm ye-10. imizuzwana.
2. Ukwandiswa kwePCR
2.1 Layisha ipleyiti yePCR elungisiweyo/iityhubhu kwisixhobo sePCR sefluorescence, Ulawulo olubi kunye nolawulo olulungileyo luya kumiselwa kuvavanyo ngalunye.
2.2 Ukusetwa kwetshaneli yeFluorescent:
1) Khetha itshaneli ye-FAM yokuchongwa kweMPV;
2) Khetha itshaneli ye-HEX/VIC yolawulo lwangaphakathi lofuzo;
3.Uhlalutyo lweziphumo
Seta umgca wesiseko ngentla kweyona ndawo iphezulu yegophe le-fluorescent yolawulo olungalunganga.
4.Ulawulo lomgangatho
4.1 Ulawulo olubi: Akukho xabiso le-Ct lifunyenwe kwi-FAM, i-HEX / VIC channel, okanye i-Ct＞40;
4.2 Ulawulo oluhle: Kwi-FAM, itshaneli ye-HEX/VIC, Ct≤40;
4.3 Ezi mfuno zingentla kufuneka zaneliswe kuvavanyo olufanayo, kungenjalo iziphumo zovavanyo azivumelekanga kwaye uvavanyo kufuneka luphindwe.
【Sika ixabiso】
Isampulu ithathwa njengento entle xa: Ulandelelwano ekujoliswe kulo Ct≤40, Igene yolawulo lwangaphakathi Ct≤40.
【Ukutolikwa kweziphumo】
Nje ukuba ulawulo lomgangatho lugqithiselwe, abasebenzisi kufuneka bajonge ukuba ngaba kukho ijika lokukhulisa kwisampulu nganye kwitshaneli ye-HEX/VIC, ukuba kukho kunye ne-Ct≤40, ibonise ukuba ijini yolawulo lwangaphakathi yandisiwe ngempumelelo kwaye olu vavanyo luchanekileyo. Abasebenzisi banokuqhubeka nohlalutyo olulandelayo:
3.Kwiisampulu ezinokwandiswa kwejini yolawulo lwangaphakathi ayiphumelelanga (HEX/VIC
itshaneli, Ct＞40, okanye akukho curve yokukhulisa), umthamo weViral ophantsi okanye ubukho be-PCR inhibitor bunokuba sisizathu sokungaphumeleli, uviwo kufuneka luphindwe kwingqokelela yomfanekiso;
4.Iisampulu ezintle kunye nentsholongwane ekhulisiwe, iziphumo zolawulo lwangaphakathi azichaphazeli;
Kwiisampuli ezifunyaniswe zi-negative, ulawulo lwangaphakathi kufuneka luvavanywe ukuba unayo, kungenjalo iziphumo zizonke azikho mthethweni kwaye uvavanyo kufuneka luphindwe, kuqalwa kwinyathelo lokuqokelelwa kwesampula.
Ulwazi Lomboniso
Isatifikethi sembeko
Iprofayile yekhampani
Thina, i-Hangzhou Testsea Biotechnology Co., Ltd yinkampani ekhula ngokukhawuleza yobuchwephesha bebhayoloji ekhethekileyo ekuphandeni, ekuphuhliseni, ekuveliseni nasekusasazeni iikhithi zovavanyo lwe-in-vitro diagnostic (IVD) kunye nezixhobo zonyango.
Indawo yethu yi-GMP, ISO9001, kunye ne-ISO13458 eqinisekisiweyo kwaye sinemvume ye-CE FDA. Ngoku sijonge phambili ekusebenzisaneni neenkampani zaphesheya kwezilwandle ngophuhliso olufanayo.
Sivelisa uvavanyo lokuchuma, uvavanyo lwezifo ezosulelayo, uvavanyo lokusetyenziswa kakubi kweziyobisi, uvavanyo lwentliziyo, uvavanyo lokumakisha ithumba, uvavanyo lokutya kunye nokhuseleko kunye novavanyo lwezifo zezilwanyana, ukongezelela, uphawu lwethu lwe-TESTSEALABS luye lwaziwa kakhulu kwiimarike zasekhaya naphesheya. Umgangatho ogqwesileyo kunye namaxabiso ancomekayo asenza sikwazi ukuthatha ngaphezulu kwe-50% yezabelo zasekhaya.
Inkqubo yeMveliso
1.Lungiselela
2.Ikhava
3.Inwebu enqamlezileyo
4.Sika umcu
5.Indibano
6.Pakisha iipowutshi
7.Zitywine iingxowa
8.Pakisha ibhokisi
9.Encasement
Thintela Intlekele Entsha: Lungiselela Ngoku njengoko i-Monkeypox isasazeka
Ngomhla we-14 ka-Agasti, uMbutho wezeMpilo weHlabathi (i-WHO) ubhengeze ukuba ukuqhambuka kwemonkeypox kukwenza "iMpilo yoLuntu oluNgxamisekileyo lweNgcaciso yeHlabathi." Eli lixesha lesibini i-WHO ikhupha elona nqanaba liphezulu lesilumkiso malunga nokuqhambuka kwemonkeypox ukusukela ngoJulayi ka-2022.
Okwangoku, ukuqhambuka kwemonkeypox kuye kwasasazeka ukusuka e-Afrika ukuya eYurophu nase-Asiya, ngamatyala aqinisekisiweyo axeliweyo eSweden nasePakistan.
Ngokutsho kwedatha yamva nje evela kwi-Africa CDC, kulo nyaka, amazwe ali-12 angamalungu e-African Union anike ingxelo ye-18,737 yeemeko zemonkeypox, kubandakanywa namatyala angama-3,101 aqinisekisiweyo, amatyala angama-15,636 akrokrelwayo, kunye nokufa kwe-541, kunye nezinga lokufa kwe-2.89%.
01 Yintoni iNkawu?
Imonkeypox (MPX) sisifo se-viral zoonotic esibangelwa yintsholongwane yemonkeypox. Inokudluliselwa ukusuka kwizilwanyana ukuya ebantwini, kunye naphakathi kwabantu. Iimpawu eziqhelekileyo ziquka umkhuhlane, i-rash, kunye ne-lymphadenopathy.
Intsholongwane yemonkeypox ingena emzimbeni womntu ngeenwebu ze-mucous kunye nolusu olwaphukileyo. Imithombo yosulelo ibandakanya iimeko zemonkeypox kunye neempuku ezosulelekileyo, iinkawu, kunye nezinye iiprimates ezingengobantu. Emva kosulelo, ixesha lokufukanyelwa ziintsuku ezi-5 ukuya kwezi-21, ngokwesiqhelo iintsuku ezi-6 ukuya kwezi-13.
Nangona uluntu ngokubanzi luchaphazeleka kwintsholongwane yemonkeypox, kukho inqanaba elithile lokhuseleko lokuwela inkawu kwabo bagonyelwe ingqakaqha, ngenxa yokufana kofuzo kunye ne-antigenic phakathi kweentsholongwane. Okwangoku, i-monkeypox isasazeka phakathi kwamadoda alala namadoda ngokunxibelelana ngesondo, ngelixa umngcipheko wosulelo kuluntu ngokubanzi uhlala uphantsi.
02 Kwahluke njani oku kuqhambuka kweMonkeypox?
Ukususela ekuqaleni konyaka, uhlobo oluphambili lwentsholongwane ye-monkeypox, "i-Clade II," ibangele ukuqhambuka okukhulu kwihlabathi jikelele. Okukhathazayo kukuba, inani leemeko ezibangelwa ngu-"Clade I," eziqatha kakhulu kwaye zinezinga eliphezulu lokufa, liyenyuka kwaye liqinisekisiwe ngaphandle kwelizwekazi laseAfrika. Ukongeza, ukusukela ngoSeptemba kulo nyaka uphelileyo, uhlobo olutsha, oluyingozi ngakumbi kwaye ludluliselwa ngokulula, "Clade Ib,” sele iqalile ukusasazeka kwiDemocratic Republic of the Congo.
Into eqaphelekayo kolu qhambuko kukuba abasetyhini nabantwana abangaphantsi kweminyaka eyi-15 ngabona bachaphazeleka kakhulu.
Idatha ibonisa ukuba ngaphezu kwe-70% yeemeko ezixeliweyo kwizigulane ezingaphantsi kweminyaka eyi-15 ubudala, kwaye phakathi kweemeko ezibulalayo, eli nani linyuka ukuya kuma-85%. Ngokucacileyo,izinga lokufa kwabantwana liphezulu ngokuphindwe kane kunabantu abadala.
03 Yintoni uMngcipheko woSulelo lweMonkeypox?
Ngenxa yexesha labakhenkethi kunye nokusebenzisana rhoqo ngamazwe ngamazwe, umngcipheko wokusasazwa kwemida ye-monkeypox virus unokwanda. Nangona kunjalo, intsholongwane isasazeka ngokusondelelana ixesha elide, njengokuthatha inxaxheba kwezesondo, ukudibana kwesikhumba, kunye nokuphefumla ngokusondeleyo okanye ukuthetha nabanye, ngoko ke amandla ayo okusasaza umntu ukuya emntwini abuthathaka.
04 UyiNqanda Njani iNkawu?
Kulumkele ukwabelana ngesondo nabantu abangaziwayo isimo sabo sempilo. Abahambi kufuneka bathathele ingqalelo ukuqhambuka kwemonkeypox kumazwe abasingise kuwo kunye nemimandla kwaye baphephe ukunxibelelana neempuku kunye neeprimates.
Ukuba ukuziphatha okusemngciphekweni omkhulu kwenzeka, zijonge impilo yakho kangangeentsuku ezingama-21 kwaye ugweme ukunxibelelana ngokusondeleyo nabanye. Ukuba iimpawu ezifana nerhashalala, amadyungudyungu, okanye umkhuhlane ziyavela, funa unyango ngokukhawuleza kwaye uxelele ugqirha ngokuziphatha okufanelekileyo.
Ukuba ilungu lentsapho okanye umhlobo ufunyaniswa ene-monkeypox, thabatha amanyathelo okuzikhusela, kuphephe ukusondelelana nomguli, kwaye ungachukumisi izinto ezisetyenziswe ngumguli, njengempahla, ibhedi, iitawuli kunye nezinye izinto zobuqu. Kuphephe ukusebenzisa amagumbi okuhlambela, kwaye uhlambe izandla rhoqo kunye namagumbi okukhupha umoya.
I-Monkeypox Diagnostic Reagents
I-Monkeypox yokuxilonga i-reagents inceda ukuqinisekisa ukusuleleka ngokufumanisa i-antigens ye-viral okanye i-antibodies, ivumela ukuhlukaniswa okufanelekileyo kunye neendlela zonyango, kunye nokudlala indima ebalulekileyo ekulawuleni izifo ezithathelwanayo. Okwangoku, i-Anhui DeepBlue Medical Technology Co., Ltd. iphuhlise i-reagents yokuxilongwa kwemonkeypox ilandelayo:
I-Monkeypox Antigen Test Kit: Isebenzisa indlela yegolide ye-colloidal ukuqokelela imizekelo efana ne-oropharyngeal swabs, i-nasopharyngeal swabs, okanye i-exudates yesikhumba ukuze ibonwe. Iqinisekisa usulelo ngokubona ubukho bentsholongwane yentsholongwane.
Ikhithi yoVavanyo lweMonkeypox Antibody: Isebenzisa indlela yegolide ye-colloidal, kunye neesampulu ezibandakanya igazi elipheleleyo le-venous, iplasma, okanye iserum. Iqinisekisa usulelo ngokufumanisa izilwa-buhlungu eziveliswa ngumzimba womntu okanye wesilwanyana ngokuchasene nentsholongwane yemonkeypox.
I-Monkeypox Virus Nucleic Acid Test Kit: Isebenzisa indlela ye-PCR yexesha lokwenene le-fluorescent, kunye nesampulu ye-lesion exudate. Iqinisekisa usulelo ngokufumanisa ijenome yentsholongwane okanye amaqhekeza emfuza athile.
Testsealabs 'Monkeypox Testing Products
Ukusukela ngo-2015, ii-reagents zokuxilonga ze-Testsealabs 'monkeypox ziye zaqinisekiswa kusetyenziswa iisampulu zentsholongwane yokwenyani kwiilabhoratri zangaphandle kwaye ziye zaqinisekiswa nge-CE ngenxa yokusebenza kwazo okuzinzile nokuthembekileyo. Ezi reagents zijolise kwiintlobo ezahlukeneyo zesampulu, zibonelela ngobuntununtunu kunye namanqanaba athile, zibonelela ngenkxaso eyomeleleyo yokubhaqwa kosulelo lwemonkeypox kunye noncedo olungcono kulawulo olusebenzayo lokuqhambuka. Ngolwazi oluthe kratya malunga nekhithi yethu yovavanyo lwemonkeypox, nceda ujonge kwakhona: https://www.tesselabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Inkqubo yovavanyo
Ukusebenzisa i-swab ukuqokelela ubovu kwi-pustule, ukuxuba ngokucokisekileyo kwibuffer, kwaye ke usebenzisa amathontsi ambalwa kwikhadi lovavanyo. Isiphumo sinokufunyanwa ngamanyathelo ambalwa nje alula.
