Intsholongwane ye-Monkeypox intsholongwane (i-MPV) ye-NUCEIC ye-Acid
Intshayelelo
Ikhithi isetyenziselwa ukuboniswa komgangatho we-vitroy virus otyokiweyo kwe-Monkey ye-Monkey), amatyala adityanisiweyo kunye namanye amatyala afuna ukufunyaniswa kwi-Monkey ye-Monkeypox.
Ikhithi isetyenziselwa ukufumana i-F3l Gene ye-MPV ePhaphukayo iShaphy kunye neesampulu ze-nasal swab.
Iziphumo zovavanyo zale kit zeyokukoreji yekliniki kuphela kwaye akufuneki zisetyenziswe njenge-provision kuphela yesifo sekliniki. Kucetyiswa ukuqhuba uhlalutyo olubanzi lwemeko esekwe kwikliniki yesigulana
ukubonakaliswa kunye novavanyo lweelebhu.
Ukusetyenziswa kwenjongo
| Uhlobo lwe-Assay | I-jabs swabs kunye ne-nasal swab |
| Uhlobo lovavanyo | Ukufanelekela |
| Izinto zovavanyo | I-pcr |
| Ubungakanani bepakethi | I-48Atestss / ibhokisi eyi-1 |
| Iqondo lokugcina | 2-30 ℃ |
| Beka ubomi kwishelufa | Iinyanga ezili-10 |
Inqaku lemveliso
Umgaqo
Le kit ithatha ulandelelwano oluthile oluthile lwe-MPV f3l njengendawo ekujoliswe kuyo. Itekhnoloji yokufumana i-FCR ye-PRCR ye-PRC ye-PCRCR kunye neTekhnoloji ye-Nucleic Acid isetyenziselwa ukubeka iliso kwi-acid yenyoka ye-nycleic ngotshintsho lweemveliso ze-faorescence. Inkqubo yokufunyanwa ibandakanya ulawulo lomgangatho wangaphakathi, olusetyenziselwa ukubek'esweni ukuba kukho i-PCR ethintela iisampulu okanye ukuba zithathwa ziiseli kwiisampuli, ezinokuthi zithintele ngokufanelekileyo imeko engeyiyo ngokufanelekileyo.
Izinto eziphambili
Ikhithi iqulethe i-regentnts yokuqhubela phambili iimvavanyo ezingama-48 okanye ulawulo lomgangatho, kubandakanya nezi zinto zilandelayo:
Ukuqasha a
| Igama | Izinto eziphambili | Ubungakanani |
| Ukuchongwa kwe-MPV ukuqasha | I-tube yokuphendula iqulethe i-MG2 +, I-F3L Gene / RNase p primer probe, I-Buffer yokuphendula, i-TAQ Dzyme. | Iimvavanyo ezingama-48 |
UkuqashaB
| Igama | Izinto eziphambili | Ubungakanani |
| I-MPV Ulawulo oluqinisekileyo | Iqulethe i-MPV ekujoliswe kuyo | I-1 ityhubhu |
| I-MPV Ulawulo olubi | Ngaphandle kwe-MPV ekujoliswe kuyo | I-1 ityhubhu |
| I-DNA yokukhupha i-DNA | I-regent iqulethe i-tris, e-Edta kunye neTritoni. | I-48pcs |
| Ukuphinda-phinda kwakhona kwakhona | Amanzi athathwayo | 5ml |
Qaphela: Amacandelo amanani ahlukeneyo e-batch ayinakusetyenziswa ngokudibeneyo
】Iimeko zokugcina kunye nobomi beshelfu】
I-A / B inokugcinwa nge-2-30 ° C, kwaye ubomi beshelfu ziinyanga ezili-10.
2. Nceda vula ityhubhu ye-tube yovavanyo kuphela xa sele ukulungele uvavanyo.
3.Ungasebenzisi iityhubhu zovavanyo ngaphaya komhla wokuphelelwa.
4.Ungasebenzisi ityhubhu yokufunyanwa kweqatha.
】Isixhobo esisebenzayo】
Ilungele i-LC48 yenkqubo yohlalutyo lwe-LC480 ye-PCR, inobude be-VCR ye-PCR, i-ABI7500 ye-PCR.
】Iifoto zeSampula】
1. Iindidi zesampulu: Umqala utshabalala iisampulu.
2. Isisombululo seSonterling:Emva kokuqinisekiswa, kuyacetyiswa ukuba isebenzise ityhubhu ye-saline okanye i-virus yentsholongwane eveliswe yi-chatse chaibseya yebhanti ye-Hangzhou ikhangeli eqokeleleni.
I-Swab:Sula i-Bilatal Ponsnyanial Ponssuls kunye nodonga lwasePorteringant Paysul-Samngel of Stellling Swible, i-suble i-swab kwityhubhu ene-3ml yesampulu equlathe ityhubhu ye-3ML.
3.Susa ukugcinwa kunye nokuhanjiswa:Iisampulu ekufuneka zihlolwe kufuneka zivavanywe ngokukhawuleza. Iqondo lokuhamba lokuhamba kufuneka ligcinwe kwi-2 ~ 8 ℃ .Iisampulu ezinokuvavanywa ngaphakathi kweeyure ezingama-24 zinokugcinwa kwi-2 ℃ ~ 8 ~ kwaye ukuba iisampulu azinakuvavanywa kwiiyure ezingama-24, kufuneka zigcinwe ngaphantsi okanye zingalingani ukuya -70 ℃ (ukuba akukho meko yendawo yokugcina -70 ℃, inokugcinwa nge-20 ℃ okwethutyana), kuphephe ukuphindaphindwa
Ukuqaqanjelwa kunye nokucheba.
4. Ukuqokelelwa kwesampulu, ukugcinwa, kunye nokuhamba kubalulekile ekusebenzeni kwale mveliso.
】Indlela yovavanyo】
I-1.Sleple iqhume kunye nesampulu yongezwa
1.1 Ukulungiswa kwesampulu
Emva kokuxubeka esi sisombululo sesampuluni ngasentla kunye neesampulu, thatha i-30μl yesampulu kwi-DNA yokukhutshwa kwe-DNA Ragent ityhubhu kwaye uxube ngokulinganayo.
1.2 Ukulayisha
Thatha i-20μl ye-regent yokuhlengahlengisa kwakhona kwaye uyongeze kwi-MPV yokufunyanwa kwe-MPV, yongeza kwi-5μ ye-5μ ye-5μ ye-5μ ye-5μ yenziwa ngokuchaseneyo neesampulu ze-cebe, i-centrifuyiwe nge-10 imizuzwana.
I-2. I-PCR ye-purp
2.1 Layisha ipleyiti / iityhubhu ezilungiselelwe i-PRCROROROROROSCENCAN, ulawulo olubi kunye nolawulo oluqinisekileyo luya kuseta uvavanyo ngalunye.
2.2 I-Fluorescence Chapt:
1) Khetha ijelo le-file ye-MPV;
2) Khetha i-hex / vic yomjelo wokuchonga ngaphakathi kolawulo lwangaphakathi;
Uhlalutyo lwe-3.REST
Beka umgca osisiseko ngaphezulu kweyona ndawo iphakamileyo ye-fluorescent curve.
Ulawulo lokufundisa
4.1 Ulawulo olubi: Akukho candelo le-CT elifunyenwe kwi-Fam, i-hex / vick channel, okanye i-CT> 40;
4.2 Ulawulo oluqinisekileyo: Kwintsapho, iHex / Vic Channel, Ct≤40;
4.3 Ezi mfuno zilapha ngasentla kufuneka zaneliswe kukovavanyo olufanayo, kungenjalo iziphumo zovavanyo azisebenzi kwaye uvavanyo kufuneka luphindwe.
】Sika ixabiso】
Isampulu ithathwa njengento elungileyo xa: ukulandelelana kwe-CT≤40, ulawulo lwangaphakathi nolawulo lwe-gene ct≤40.
】Iziphumo zokutolikwa iziphumo】
Nje ukuba ulawulo lomgangatho ludluliselwe, abasebenzisi kufuneka bajonge ukuba kukho ijika le-Amerve kwi-hex / vig itshaneli, ukuba kukho i-CT≤40, ibonise ulawulo lwangaphakathi luphele ngempumelelo kwaye olu vavanyo lusemthethweni. Abasebenzisi banokuqhubeka kuhlalutyo lokulandela:
3.Ukuzibandakanya iisampulu zolawulo lwangaphakathi gene ngaphandle kwe (hex / vem
Ijelo, i-CT> 40, okanye akukho mthwalo we-Anplice), umthwalo wentsholongwane ophantsi okanye ubukho be-PCR Toure kunokuba sisizathu sokungaphumeleli, uvavanyo kufuneka luphindaphinde kwingqokelela yentengiso;
4.Kaloku 4Ke iisampulu ezizenzayo kunye nentsholongwane ekhulisiweyo, iziphumo zolawulo lwangaphakathi azichaphazeli;
Kwiisampuli ezivavanyiweyo, ulawulo lwangaphakathi kufuneka luvavanywe ngenye indlela iziphumo ezizonke azisebenzi kwaye iimviwo kufuneka ziphindwe, ukuqala kwinyathelo lengqokelela
Ulwazi lomboniso
Isatifikethi sobungqina
Iprofayile yekhampani
Thina, i-Chenzhou Veetachnolognology Co., I-LTD yinkampani ekhulayo ye-bitechnology ekhulelweyo ekhethekileyo ekuqhubeni, ephuhlisa, ukwenziwa kunye nokusasaza i-vit yovavanyo lwe-Vitro Diacenostic (iVD).
Indawo yethu yi-GMP, i-ISO9001, kunye neSO13458 eqinisekisiweyo kwaye sinokwamkelwa kwe-FDA. Ngoku sijonge phambili ekubambeni ngeenkampani ezingapheliyo zophuhliso.
Sivelisa uvavanyo lokuchuma, uvavanyo lwezifo ezosulelayo, iimvavanyo zokusebenzisana iziyobisi, iimvavanyo zokumakisha i-Cardiac, iimvavanyo zokumakisha i-goumoc, iimvavanyo zokutya kwe-tumor, uvavanyo lwezifo zethu, ukongeza, iimvavanyo zethu zokutya, ukongeza kwimarike yezasekhaya. Eyona ndawo ilungeleleneyo kunye namaxabiso afanelekileyo esenza ukuba sithathe ngaphezulu kwe-50% izabelo zasekhaya.
Inkqubo yemveliso
1.Topt
2.Cover
3.cross membrane
4.Sut Strip
5.assebyly
6.Packck iipakethi
7.Feal iipakethi
8.pack ibhokisi
I-Khupha
Thintela intlekele entsha: Lungiselela ngoku njengoko iMonkeypox isasazeka
Nge-14 ka-Agasti, iMbutho yeMpilo yeHlabathi (i-WHO) ibhengeza ukuba ukuqhushumba kwe-Monketpox yenza "imeko kawonkewonke yezempilo yenkxalabo yeHlabathi." Eli lixesha lesibini elikhuphe elona nqanaba liphezulu lokwazisa malunga ne-Monkeypox ukusuka ekuqaleni kukaJulayi 2022.
Okwangoku, ukuqhambuka kwenkawu kusasazeke ukusuka e-Afrika ukuya eYurophu naseAsia, ngamatyala aqinisekisiweyo axelwe eSweden nasePakistan.
Ngokwedatha yakutshanje kwi-CDC, kulo nyaka, ilungu le-12 le-African Union liye lanika amatyala angama-18,737 e-Monkey13, kubandakanya amatyala angama-3,836, kunye nenqanaba lokufa eli-2,89%.
01 Yintoni inkawu?
I-Monkeypox (MPX) sisifo se-jimuratic soomotic esibangelwa yintsholongwane ye-Monkeypox. Inokudluliselwa kwizilwanyana ebantwini, kunye phakathi kwabantu. Iimpawu eziqhelekileyo zibandakanya umkhuhlane, i-rash, kunye ne-lymphadenopathy.
Intsholongwane ye-Monkeypox ikakhulu ingena emzimbeni womntu ngokusebenzisa i-mucous membrane kunye nolusu olwaphukileyo. Imithombo yosulelo ibandakanya amatyala enkawu kwaye iintonga ezosulelekileyo, iinkawu, kunye nezinye izinto ezingezomntu. Emva kosuleleko, ixesha lokufakwa ngokufihlakeleyo ziintsuku ezi-5 ukuya kwezingama-21, ngokuqhelekileyo iintsuku ezi-6 ukuya kwezi-13.
Nangona uninzi lwabemi ngokubanzi luchaphazeleka kwintsholongwane ye-Monkeypox, kukho inqanaba elithile lokukhuselwa kwe-Monkey kwi-Monkeypox kwabo bagonywe ngokuchasene ne-genespox, ngenxa yokufana ne-antigenic phakathi kwentsholongwane. Okwangoku, inkawu isasazeka phakathi kwamadoda abelana ngesondo nabantu ngokunxibelelana ngesondo, ngelixa umngcipheko wokosuleleka kubantu ngokubanzi uhlala ephantsi.
I-02 injani le nkabukhwe inkawu?
Ukususela ekuqaleni konyaka, eyona nto iphambili kwintsholongwane enkulu ye-MonkeyKoxpox, "waqubisana ne-II," kubangele ukuqhuma okukhulu emhlabeni wonke. Ngokukhathazeka, umyinge wamatyala obangelwe "crode i," oqatha kwaye unezinga eliphakamileyo kakhulu, uyanda kwaye ungqinisisiwe ngaphandle kwelizwekazi lase-Afrika. Ukongeza, ukusukela ngoSeptemba Kunyaka ophelileyo, ukuba yinto entsha, engakumbi ebolekileyo eyahlukileyo, "Clade IB, "uqalile ukusasazeka kwiRiphabhlikhi yeDemocy yeCongo.
Inqaku elibonakalayo le qaqobana kukuba abafazi nabantwana abangaphantsi kweminyaka eli-15 bachaphazeleka kakhulu.
Idatha ibonisa ukuba ngaphezulu kwe-70% yamatyala axeliweyo akwizigulana ezingaphantsi kweminyaka eli-15, kwaye phakathi kwamatyala abulalayo, eli nani likhuphuka line-85%. Ngokukodwa,Izinga lokufa kwabantu ngamaxesha amane aphezulu kunabantu abadala.
03 Yintoni umngcipheko wokosulelwa yi-Monkeypox?
Ngenxa yexesha labakhenkethi kunye nokusebenzisana rhoqo kwamanye amazwe, umngcipheko wokuhanjiswa komda we-Monkey Nangona kunjalo, intsholongwane ikakhulu isasazeka ngokusondeleyo, njengokwesondo, ukunxibelelana kwesikhumba, kunye nokuphefumla okusondeleyo, ke umntu ohambisa umntu ngamnye - umntu ohambisa umntu ngamnye abuthathaka.
04 Indlela yokuthintela i-Monkeypox?
Kulumkele ukunxibelelana ngesondo nabantu abayaziwayo bezempilo. Abahambi kufuneka banikele ingqalelo kwi-robkeypox kumazwe abo kunye nemimandla yabo kwaye uphephe ukunxibelelana neentonga kunye nepristates.
Ukuba indlela yokuziphatha emngciphekweni omkhulu yenzeka, ukuzibeka ekubekeni impilo kwiintsuku ezingama-21 kwaye uphephe ukunxibelelana ngokusondeleyo nabanye. Ukuba iimpawu ezinje ngokungxama, i-fasts, okanye umkhuhlane, funa unyango ngokukhawuleza kwaye wazise ugqirha wendlela efanelekileyo yokuziphatha.
Ukuba ilungu losapho okanye umhlobo ufunyaniswe ene-Monkeypox, athabathe amanyathelo okhuselo, thintela unxibelelwano olusondeleyo nesigulana esisetyenzisiweyo, njengokunxiba, iitawuli, kunye nezinye izinto zobuqu. Kulumkele ukwabelana ngeenyawo, kwaye hlamba rhoqo izandla kunye namagumbi omzimba.
I-ReageOction Reagestic Reagestic
I-Remegenolostic Reagenolostic Reagenestistic Adgents inceda usulelo ngokufumana i-anticral atugens okanye ii-antibodies, inika indawo efanelekileyo yokuzahlula-hamba, kwaye idlale indima ebalulekileyo ekulawuleni izifo ezosulelayo. Okwangoku, i-anui inzululwazi yeTekhnoloji yeOnyango CO., Ltd. iphuhlise le mivuzo ilandelayo ye-nickeypox:
I-MonkeyoketPox Antigen Vikit Kit: Isebenzisa indlela yeGolide yeGolide yokuqokelela iisampuli ezinje nge-Oropharyngeal Swabs, okanye ulusu olucekeceke. Iqinisekisa ukosuleleka ngokufumana ubukho be-vieral andultion.
I-Monkeypox Anti kikhi yovavanyo lwe-Monkey Iqinisekisa ukosuleleka ngokufumana ii-antibodies eziveliswe ngumntu okanye umzimba wesilwanyana kwintsholongwane yeMonkeypox.
I-Monkeyoketpox intsholongwane intsholongwane ye-Nulecy Ikhithi yovavanyo lwe-Acid: Isebenzisa i-Fluorestial Fluorestice Cluorestial Pob ye-PRCR, ngesampulu yokuba i-lesion ihlule. Iqinisekisa ukosuleleka ngokufumana i-genome yentsholongwane okanye iziqwenga ezithile zemfuza.
Iimveliso zovavanyo lwe-VeestSEAHPS
Ukusukela ngo-2015, i-vistSekabs yovavanyo lwe-vistsselepox iqinisekisiwe kusetyenziswa iisampulu zentsholongwane kwilebhu yangaphandle kwaye iqinisekiswe ngenxa yokusebenza kwabo nokuzinza kwabo. Ezi regentnts zijolise kwiindidi ezahlukeneyo zesampulu, zinikela ubuntununtunu kunye namanqanaba aphezulu, zibonelela ngenkxaso eqinileyo yokuchonga inkangeleko yosulelo kunye nokuncedisa kulawulo olusebenzayo olusebenzayo. I-FARE INKCAZISO ENGCACISO YOVAVANYO LWEXELI LWEXABISO LOVAVANYO LOKUQALA, nceda uphonononge: https ///pp.testSEalabs.com/monkes.com
Inkqubo yovavanyo
Sebenzisa i-swab ukuqokelela i-pus ukusuka kwi-Pustule, ukuxuba ngokucokisekileyo kwibuffer, emva koko usebenzise amathontsi ambalwa kwikhadi lovavanyo. Isiphumo sinokufumaneka kumanyathelo nje alula alula.
