Ikhasethi yoVavanyo lweMonkeyPox Antigen (iSerum/Plasma/Swabs)

Inkcazelo emfutshane:

Inkawusisifo zoonotic ezibangelwa yintsholongwaneIntsholongwane yeMonkeypox, eyeyeUhlobo lwe-OrthopoxvirusyePoxviridae usapho. Ngelixa ifana nengqakaqha, imonkeypox ayibabi kakhulu kwaye inezinga lokusweleka elisezantsi. Intsholongwane yafunyanwa okokuqala kwi1958kwiinkawu zaselabhorethri (kungoko igama), kodwa ngoku yaziwa ngokuyintloko kuchaphazela iimpuku kunye nezinye izilwanyana. Esi sifo saqala ukuxelwa ebantwini1970kwiIRiphabliki yaseDemocratic Congo.

I-monkeypox inokudluliselwa ebantwini ngokusebenzisa:

Uqhagamshelwano oluthe ngqonezilwanyana ezosulelekileyo (umzekelo, ukuphatha inyama yasendle).
Usulelo kumntu ukuya emntwiningokusebenzisa amathontsi okuphefumla, ukudibana ngokusondeleyo nolwelo lomzimba, okanye izilonda zolusu.
Into ekwazi ukudlulisela izinto ezosulelayo(izinto ezingcolisekileyo okanye umphezulu).

Iimpawu zemonkeypox ebantwini zifana nezo zeingqakaqha, kuquka:

Ifiva
Intloko ebuhlungu
Izihlunu ezibuhlungu
Ukudinwa
IiRashes, ngokuqhelekileyo iqala ebusweni kwaye isasazeke kwamanye amalungu omzimba, ihlala ikhula ibe yizilonda ezizaliswe lulwelo (irhashalala).

Thumela i-imeyile kuthi Khuphela njengePDF

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iinkcukacha zeMveliso:

Uvakalelo oluPhezulu kunye nokuNgcaciswa
Uvavanyo lwenzelwe ukunika ubhaqo oluchanekileyo lweIintsholongwane ze-monkeypox antigens okanye amajoni omzimba, kunye ne-cross-reactivity encinci kunye nezinye iintsholongwane ezifanayo.
Iziphumo eziKhawulezayo
Iziphumo ziyafumaneka ngaphakathi15-20 imizuzu, iyenza ilungele ukwenza izigqibo ngokukhawulezaizicwangciso zeklinikhiokanye ngexesha lokuqhambuka.
Ukusebenziseka lula
Uvavanyo lusebenziseka lula kwaye alufuni uqeqesho olulodwa okanye izixhobo. Ifanelekile ukusetyenziswa ngabasebenzi bezempilo kwiindawo ezahlukeneyo, kuqukaamagumbi kaxakeka, iikliniki zezigulane ezingalaliswayo, kwayeizibhedlele ebaleni.
Iintlobo zeeSampuli eziNyenzekayo
Uvavanyo luyahambelanaigazi elipheleleyo, serum, okanyeiplasma, enikezela ubhetyebhetye ekuqokeleleni isampulu.
Ephathekayo kwaye Ilungele Ukusetyenziswa Endle
Uyilo oludibeneyo lovavanyo luyenza ilungele ukusetyenziswa kwiiiyunithi zempilo ezihambayo, iinkqubo zokufikelela eluntwini, kwayeiimeko zokusabela ubhubhane.

Umgaqo:

II-Monkeypox Rapid Test Kitisebenza kumgaqo welateral flow immunochromatography, apho uvavanyo lufumanisa nokubaIintsholongwane ze-monkeypox antigens or amajoni omzimba. Inkqubo imi ngolu hlobo lulandelayo:

Isampulu yoQokelelo
Umthamo omncinci weigazi elipheleleyo, serum, okanyeiplasmayongezwa kwisampulu yequla lesixhobo sovavanyo. Isisombululo sesithinteli siye sisetyenziswe ukuququzelela ukuhamba kwesampulu.
I-Antigen-Antibody Reaction
Ikhasethi yovavanyo iqulatheii-antigens eziphinda zihlangane or amajoni omzimbangqo kwintsholongwane yeMonkeypox. Ukuba isampuli iqulethe i-Monkeypox virus-specificamajoni omzimba(IgM, IgG) okanyeii-antigensukusuka kusulelo olusebenzayo, baya kubophelela kwicandelo elihambelanayo kuluhlu lovavanyo.
Ukufuduka kweChromatographic
Isampuli ihamba ecaleni kwenwebu ngenxa yesenzo se-capillary. Ukuba i-Monkeypox-specific antigens okanye i-antibodies ikhona, baya kubopha kumgca wokuvavanya (umgca we-T), ukuvelisa ibhendi enemibala ebonakalayo. Ukuhamba kwee-reagents kwakhona kuqinisekisa ukubunjwa kwe-aumgca wolawulo (umgca C), eqinisekisa ukunyaniseka kovavanyo.
Isiphumo sokutolika
- Imigca emibini (T line + C line):Isiphumo esihle, esibonisa ubukho be-Monkeypox virus antigen okanye amajoni omzimba.
- Umgca omnye (umgca C kuphela):Isiphumo esibi, esibonisa ukuba akukho antigen yentsholongwane yeMonkeypox okanye amajoni omzimba.
- Akukho mgca okanye u-T kuphela:Iziphumo ezingasebenziyo, zifuna ukuvavanywa kwakhona.

Ukuqulunqwa:

Ukuqamba	Isixa	Inkcazo
IFU	1	/
Ikhasethi yovavanyo	25	Isingxobo ngasinye sefoil esitywiniweyo esinesixhobo esinye sovavanyo kunye ne-desiccant enye
Ukukhutshwa kwe-diluent	500μL1 Umbhobho 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Ingcebiso yedropha	/	/
Swab	25	/

Inkqubo yoVavanyo:


1. Hlamba izandla zakho	2. Tshekisha iziqulatho zekhithi phambi kovavanyo, bandakanya iphakheji efakiweyo, ikhasethi yovavanyo, i-buffer, iswab.	3.Beka ityhubhu yokukhupha kwindawo yokusebenzela.	4.Hlubula isitywina sefoyile ye-aluminiyam ukusuka phezulu kumbhobho wokutsalwa oqulethe isithinteli sokutsalwa.

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5.Susa ngokucokisekileyo iswab ngaphandle kokuchukumisa incam.Faka yonke incam yeswab 2 ukuya kwi-3 cm kwimpumlo yasekunene.Qaphela indawo ekuqhekeka kuyo i-swab yempumlo.Ungakuva oku ngeminwe yakho xa ufaka i-swab yeempumlo okanye ujonga. oko kusemncinane. Hlikihla ingaphakathi lempumlo xa usenza istyhula izihlandlo ezi-5 kangangemizuzwana eyi-15, thatha kwaempumlo enye uyifake kwenye impumlo. Nceda wenze uvavanyo ngokuthe ngqo kunye nesampuli kwaye ungenzi
yiyeke imile.

6.Faka i-swab kwityhubhu yokutsalwa. Jikelezisa i-swab malunga nemizuzwana ye-10, Jikelezisa i-swab kwityhubhu yokutsalwa, ucinezele intloko ye-swab ngaphakathi kumbhobho ngelixa ucinezela amacala ombhobho ukukhulula ulwelo oluninzi. ngokusemandleni ukusuka kwi-swab.


7. Thatha i-swab kwiphakheji ngaphandle kokuchukumisa i-padding.	8.Hlanganisa ngokucokisekileyo ngokucofa ngaphantsi kombhobho.Beka i-3 yehla yesampuli ngokuthe nkqo kwisampulu yomthombo wekhasethi yovavanyo.Funda umphumo emva kwemizuzu eyi-15. Qaphela:Funda isiphumo kwisithuba semizuzu engama-20. Kungenjalo, sisicelo sovavanyo siyacetyiswa.

Iziphumo Utoliko: