I-Monkeypox ye-Antigen Antigen Chatsette Cassette (i-serum / iplasma / i-swabs)

I-Monkeypox Antigen Uvavanyo lwekhasethi (i-serum / iplasma / i-swabs) iboniswe umfanekiso

Inkcazo emfutshane:

I-Monkeypoxsisifo se-vill soomotic esibangelwa yiIntsholongwane ye-Monkeypox, yeyoI-orthopoxvirus genusyeI-Poxvididae yosapho. Ngelixa ifana ne-incoxpox, i-Monkeypox ngokubanzi ibaluleke kakhulu kwaye inezinga eliphantsi lokufa. Intsholongwane yafunyanwa kuqala ngaphakathiNgo-1958Kwiinkaboratori (ngenxa yegama), kodwa ngoku iyaziwa ngokuyintloko ichaphazela iintonga nezinye izilwanyana. Esi sifo saxelwa kuqala ebantwiniNgo-1970kwiI-Democratic Republic of Congo.

I-Monkeypox inokudluliselwa ebantwini:

Uqhakamshelwano ngqoNgezilwanyana ezosulelekileyo (umzekelo, ukuphatha iBushmeat).
Ukuhanjiswa komntu-umntuVielesppictorys edikekayo, unxibelelwano olusondeleyo nolwelo lomzimba, okanye izilonda zesikhumba.
Into ekwazi ukudlulisela izinto ezosulelayo(izinto ezingcolisekileyo okanye umphezulu).

Iimpawu ze-Monkeypox ebantwini zifana nezoincinci, kubandakanya:

Ifiva
Intloko ebuhlungu
Ii-accles
Ukudinwa
Rhabaxa, ngokwesiqhelo siqala ebusweni kwaye sasasazeka kwamanye amalungu omzimba, ihlala ikhula kwizilonda ezigcwalisiweyo (POX).

Thumela i-imeyile kuthi Khuphela njenge-PDF

Iinkcukacha zemveliso

Iimpawu zeMveliso

Iinkcukacha zemveliso:

Ubuntununtunu obuphezulu kunye nobunye
Uvavanyo lwenzelwe ukubonelela ngokuchanekileyoI-Monkeypox intsholongwane ye-agobns okanye ii-antibodies, ngokusebenza okuncinci komnqamlezo kunye nezinye iintsholongwane ezifanayo.
Iziphumo eziPhuculweyo
Iziphumo ziyafumaneka ngaphakathiImizuzu eli-15 ukuya kwengama-20, yenze ukuba ilungele ukwenziwa kwezigqibo ngokukhawulezaUseto lweklinikhiokanye xa kuqhekeke.
Ukunciphisa ukusetyenziswa
Uvavanyo lusebenziseka lula kwaye alufuni kuqeqesho olukhethekileyo okanye izixhobo. Ilungele ukusetyenziswa ngoochwephesha bezempilo kwizicwangciso ezahlukeneyo, kubandakanyaamagumbi kaxakeka, Iiklinikhi zangaphandle, kwayeIzibhedlele zentsimi.
Iindidi zesampulu
Uvavanyo luyahambelanaIgazi liphela, i-serum, okanyeiplasma, ukucela ukuguquguquka kuqokelelo lwesampula.
Iphathwayo kwaye ilungele ukusetyenziswa kwentsimi
Uyilo lweColding lovavanyo lwenza ukuba lube lulungele ukusetyenziswa kwiIiyunithi zempilo, Iinkqubo zokufikelela eluntwini, kwayeKwiimeko zokuphendula.

Umgaqo:

II-Monkeypox ekhawulezileyo yeKitisebenza ngokomgaqo-sisekoI-LateralOchromatrographrography, apho uvavanyo lubona khonaI-Monkeypox intsholongwane i-anyanisi or ii-antibodies. Inkqubo yile ilandelayo:

Ukuqokelelwa kwesampulu
Umthamo omncinci weIgazi liphela, i-serum, okanyeiplasmayongezwa kwimodeli yesixhobo sovavanyo. Isisombululo se-buffer siyasetyenziswa ukuze kuququzelele ukuhamba kwesampulu.
I-Antigen-Anticter Read
I-Cassette yovavanyo iqulathePhinda ujonge i-adogens or ii-antibodiesngqo kwintsholongwane ye-Monkeypox. Ukuba isampulu inentsholongwane ye-Monkeypox-ngqoii-antibodies(I-IGM, i-Igg) okanyei-antigensUkusuka kusulelo olusebenzayo, baya kubophelela kwicandelo elihambelanayo kuvavanyo lovavanyo.
Ukufuduka kweChromatographic
Isampulu ihamba ecaleni kwe-membrane ngenxa yesenzo se-capillary. Ukuba i-adobypox-i-adogens ekhethekileyo okanye ii-antibodies zikhona, ziya kubambisa kumgca wokuvavanywa (umgca), zivelisa ibhendi enemibala ebonakalayo. Intshukumo yeRegednts ikwaqinisekisa ukwenziwa kweUmgca wokuLawula (C Umgca), okuqinisekisa ubunyani bovavanyo.
Isiphumo sokutolika
- Imigca emibini (t Line + C Umnxeba):Isiphumo esihle, esibonisa ubukho bentsholongwane ye-Monkeypox i-antign okanye ii-antibodies.
- Umgca omnye (c umgca kuphela):Isiphumo esibi, sibonisa ukuba akukho ntsholongwane i-Monkeypox intsholongwane ye-antign okanye ii-antibodies.
- Akukho mgca okanye umgca kuphela:Isiphumo esingasebenziyo, sifuna ukuphindaphinda.

Ukuqulunqwa:

Ukwakhiwa	Isixa	Ukucaciswa
Ukuba	1	/
Vavanya ikhasethi yekhasethi	25	Ingxowa nganye etywiniweyo enesixhobo esinye sovavanyo kunye nesidima esinye
Ukukhupha i-disent	I-500s * I-1 ye-tube * 25	I-Tris-Cl buffer, i-NACL, i-NP 40, iProtlin 300
I-Dropper Tiphu	/	/
Swab	25	/

Inkqubo yovavanyo:


1. Hlamba izandla zakho	2. Jonga iziqulatho zeKhithi ngaphambi kovavanyo, zibandakanya ukufaka iphakheji, i-Cassette yovavanyo, i-buffer, i-swab.	3.Phakamisa ityhubhu yokukhupha kwindawo yokusebenza.	4.Umcimilili we-Aluminium Foil ovela phezulu kwityhubhu yokukhupha equlethe i-buffer yokukhupha.

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5. Ngokuyisela i-swab ngaphandle kokuchukumisa i-TIP.CECTE inqaku lonke le-swab 2 ukuya kwi-3 cm ukuya kwi-nostril swabl swab. kwi-mimnor. Hlikihla ngaphakathi kwempumlo kwi-Istrings kwiintshukumo ezi-5 ubuncinci imizuzwana eli-15, ngoku thatha i-swab efanayo kwaye uyifake i-nostrils. Nceda wenze uvavanyo ngqo kwisampulu kwaye ungabi
yiyeke imi.

6. Indawo ye-swab kwi-cube yokukhupha. I-Swab malunga nemizuzwana eli-10, ijikeleze i-swab ngokuchasene ne-tybhu ye-tybhu ngelixa inyibilika ityhubhu yokukhulula ulwelo ngokusemandleni e-swab.


7. Khupha i-swab ukusuka kwiphakheji ngaphandle kokuchukumisa i-padding.	8.Mix ngokucokisekileyo ngokufihla amazantsi e-tube.ble 3 amaconsi emo yesampulu ethe nkqo kwi-capsette yovavanyo.reat isiphumo emva kwemizuzu eli-15. Qaphela: Funda isiphumo ngaphakathi kwemizuzu engama-20.OMezinye, kuyacetyiswa uvavanyo lovavanyo.

Iziphumo: