Ikhithi yovavanyo ye-ICH-CPV-CDV IgG

I-CAINE INFECTIOUS HEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ikhithi yovavanyo ye-ICH/CPV/CDV IgG) yenzelwe ukuvavanya isiqingatha sobungakanani benja ye-IgG ye-antibody ye-Canine Infectious Hepatitis Virus (ICH), Canine Parvo (CPV Parvo) kunye neCanine Distemper Virus(CDV).

IZIQULATHO ZEKHITHI

UYILO NOMGAQO

Kukho amacandelo amabini afakwe kwi-cartridge nganye: Isitshixo, esifakwe kunye ne-desiccant kwindawo ephantsi etywinwe nge-aluminium ekhuselayo, kunye nokuphuhlisa izisombululo, ezifakwe ngokwahlukileyo kwiindawo eziphezulu ezifakwe kwi-aluminium foil ekhuselayo.

Ikhatriji nganye iqulethe zonke ii-reagents eziyimfuneko kwisampula enye yovavanyo. Ngokufutshane, xa iSitshixo sifakwe kwaye sifakwe kwimizuzu embalwa kwi-compartment 1 ephezulu, apho isampuli yegazi ifakwe khona, i-antibodies ethile ye-IgG kwisampuli yegazi ehlanjululweyo, ukuba ikhona, iya kubophelela kwi-ICH, CPV okanye CDV recombinant antigens immobilized kwiindawo ezahlukeneyo discrete kwi Key efakiweyo. Emva koko iSitshixo siya kudluliselwa kumacandelo aphezulu aseleyo ngamaxesha amisiweyo inyathelo ngenyathelo. I-antibodies ye-IgG edibeneyo kwiindawo iya kubhalwa kwi-compartment ephezulu ye-3, equlethe i-anti-canine IgG enzyme conjugate kunye neziphumo zokugqibela ezivezwa njengamabala amfusa-blue kwiSitshixo ziya kuphuhliswa phezulu.

I-compartment 6, equlethe i-substrate. Ukufumana isiphumo esanelisayo, amanyathelo okuhlamba aziswa. Kwi-compartment ephezulu ye-2, i-IgG engapheliyo kunye nezinye izinto ngaphakathi kwesampuli yegazi ziya kususwa. Kwi-compartment ephezulu 4 kunye ne-5, i-unbounded or

Ukugqithiswa kwe-anti-canine IgG enzyme conjugate iya kupheliswa ngokwaneleyo. Ekugqibeleni, kwi-compartment ephezulu ye-7, i-chromosome engaphezulu iphuhliswe kwi-substrate kunye ne-enzyme edibeneyo i-conjugate kwi-compartment ephezulu ye-6 iya kususwa. Ukuqinisekisa ukunyaniseka kwentsebenzo, iprotheni yokulawula ifakwe kwindawo ephezulu kakhulu kwiSitshixo. Indawo kumbala omfusa-blue kufuneka ibonakale emva kokugqiba inkqubo yovavanyo oluyimpumelelo.

UKUGCINA

1. Gcina ikiti phantsi kwefriji eqhelekileyo (2 ~ 8℃).

MUSA UKUYIMKHETHISA IKHITHI.

2. Ikhithi iqulethe imathiriyeli yebhayoloji engasebenziyo. Ikhithi kufuneka iphathwe

kwaye ilahlwe ngokuhambelana neemfuno zococeko lwasekhaya.

INKQUBO YOVAVANYO

Ukulungiselela phambi kokwenza uvavanyo:

1. Zisa i-cartridge kwiqondo lokushisa lokushisa (20℃-30℃) kwaye uyibeke kwibhentshi yomsebenzi de i-label ye-thermal eludongeni lwe-cartridge ibe ngumbala obomvu.

2.Beka i-tissue paper ecocekileyo kwibhentshi yomsebenzi yokubeka iSitshixo.

3.Lungisa i-10μL yokukhupha kunye ne-10μL iingcebiso eziqhelekileyo zepayipi.

4. Susa ifoyile ye-aluminiyam ekhuselayo esezantsi kwaye uphose iSitshixo ngaphandle kwecandelo elisezantsi lekhatriji kwiphepha lethishu elicocekileyo.

5. Yima nkqo ikhatriji kwibhentshi yomsebenzi kwaye uqinisekise ukuba amanani ekhompatimenti aphezulu angabonwa kwicala elichanekileyo (izitampu zamanani ezichanekileyo zijonge kuwe). Cofa ikhatriji kancinci ukuze uqiniseke ukuba izisombululo kumacandelo aphezulu zibuyela ezantsi.

Ukwenza uvavanyo:

1.Ukutyhila i-foil ekhuselayo kumacandelo aphezulu ngononophelo kunye nesithupha ukusuka kwesobunxele ukuya kwesokudla kude kube KUPHELA ukuveza indawo ephezulu ye-1.

2. Fumana isampuli yegazi evavanyiweyo kunye nesethi ye-dispenser usebenzisa i-tip ye-pipette ye-10μL eqhelekileyo.

Ukuvavanya i-serum okanye i-plasma sebenzisa i-5μL.

Ukuvavanya sebenzisa igazi elipheleleyo i-10μL.

I-EDTA okanye i-heparin anticoagulant tubes iyacetyiswa kwi-plasma kunye nokuqokelela kwegazi lonke.

3. Beka isampuli kwi-compartment ephezulu 1. Emva koko uphakamise kwaye unciphise i-dispenser plunger amaxesha amaninzi ukuphumeza ukuxuba (Isisombululo esiluhlaza okwesibhakabhaka kwincam xa ukuxuba kubonisa idiphozithi eyimpumelelo).

4.Chola iSitshixo ngesiBambi seSitshixo ngomnwe ongaphambili kunye nobhontsi ngononophelo kwaye ufake isitshixo kwindawo ephezulu 1 (qinisekisa icala leqabaka leSitshixo elijongene nawe, okanye qinisekisa ukuba isangqa esikwisiBambi sisekunene xa ujongene nalo. wena). Emva koko xuba kwaye ume iSitshixo kwindawo ephezulu ye-1 yemizuzu emi-5.

5. Ukutyhila i-foil ekhuselayo ngokuqhubekayo ukuya ngasekunene de kube KUPHELA ukuveza i-compartment 2. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 2. Emva koko udibanise kwaye umise iSitshixo kwindawo ephezulu ye-2 ye-1 ngomzuzu.

6. Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de kube KUPHELA uveza ikhompatimenti 3. Thatha iSitshixo ngesiBambi kwaye ufake isitshixo kwindawo evulekileyo 3. Emva koko xuba kwaye umise iSitshixo kwindawo yesi-3 imizuzu emi-5.

7.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene kude KUPHELA ukuveza i-compartment 4. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 4. Emva koko udibanise kwaye umise iSitshixo kwindawo ephezulu ye-4 kwi-1 ngomzuzu.

8.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 5. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 5. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu ye-5 kwi-1 ngomzuzu.

9.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 6. Chola iSitshixo ngesiBambi kwaye ufake isitshixo kwindawo evulekileyo 6. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu yesi-6 imizuzu emi-5.

10.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 7. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 7. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu yesi-7 kwi-1 ngomzuzu.

11. Khupha iSitshixo kwigumbi eliphezulu lesi-7 kwaye uyomise kwiphepha lethishu malunga nemizuzu emi-5 phambi kokuba ufunde iziphumo.

Amanqaku:

Musa ukuthintela i-Frosting Side ye-Frosting Front of the Key, apho i-antigens kunye neprotheni yokulawula ingenakunyakaziswa (Uvavanyo kunye noLawulo lweNgingqi).

Kuphephe ukukrwela ukukrwela uVavanyo kunye noLawulo loMmandla ngokungqiyama elinye icala eliSmooth lesiphelo sangaphambili seSitshixo ukuya eludongeni lwangaphakathi lwekhompatimenti nganye ephezulu ngelixa udibanisa.

Ukuxuba, amaxesha e-10 aphakamisa kunye nokunciphisa iSitshixo kwindawo nganye ephezulu iyanconywa.

Veza kuphela indawo ephezulu elandelayo phambi kokudlulisela isitshixo.

Ukuba kukho imfuneko, ncamathele iiLeyibhile zeSilo esibonelelweyo kwiimvavanyo ezingaphezulu kwesinye.

UKUTOlika IZIPHUMO ZOKUVAVANYA

Jonga amabala anesiphumo kwiSitshixo ngoMbala woMbala osemgangathweni

Ayisebenzi:

AKUKHO mbala umfusa-blue ubonakala kwindawo yolawulo

Ibi(-)

AKUKHO mbala umfusa-blue ubonakalayo kwiindawo zovavanyo

Okuhle (+)

Umbala omfusa-blue obonakalayo uyavela kwiindawo zovavanyo

I-titters ye-antibodies ye-IgG ethile inokubonakaliswa ngamanqanaba amathathu