I-Fplvffvffv i-Igg yeKit

I-feline paleukonia / i-herpes virus / i-calici virus Igg yovavanyo lweKine Paleukonia Intsholongwane (FCV).

Iziqulatho

Uyilo kunye nomgaqo

Kukho izinto ezimbini ezifakwe kwikharitri nganye: Isitshixo, esibekiweyo kunye ne-ColticcalT Salathisi esekwe kwi-Aluminium ye-Aluminum i-Aluminium ye-Aluminium ye-Aluminium etywiniweyo nge-foil ekhuselweyo. Ikhareji nganye iqulethe zonke ii-redagents eziyimfuneko kuvavanyo lwesampulu enye. Ngokufutshane, xa isitshixo sifakwe ngaphakathi kwaye sibekwe kwimizuzu embalwa kwicandelo eliphezulu 1, apho isampulu yegazi ifakwe khona, ukuba ikhowudi yegazi i-IGIBIDS kwi-FPLV, i-FHV okanye I-FCV iphinde ifudumeze i-affieds engafakwanga ngokwahlukileyo

Amabala akhutshiwo kwiqhosha elifakiweyo. Emva koko isitshixo siya kudluliselwa kumacandelo aphezulu aseleyo kumanqanaba emidlalo ngamanqanaba. Ii-antibodies ezidityanisiweyo ze-IGG kumabala ziya kubizwa kwicandelo le-3, eline-Anti-Feline Igzy Godmesme kwaye iziphumo zokugqibela eziboniswe njengamabala amfuzi-valo, equlethe indawo ephezulu. Isiphumo esanelisayo, iZinyiso zokuhlalisa ziyaziswa. Kwigumbi eliphezulu lesi-2, i-IGG engabhalwanga i-IGG kunye nezinye izinto ngaphakathi kwesampulu yegazi ziya kususwa. Kwigumbi eliphezulu le-4 kunye ne-5, i-Anti-Fee-Filline-Filline Igzyme Conluvate iya kupheliswa ngokufanelekileyo. Ekupheleni, kwicandelo eliphezulu lesi-7, i-chromosome egqithisileyo yaphuhliswa ukusuka kwi-chillmed kwaye ibotshelelwe i-enzyme i-conjume kwicandelo eliphezulu 6 liya kususwa.

Ukuqinisekisa ubunyani bentsebenzo, iprotein yolawulo ingeniswa kwindawo ephezulu kakhulu kwiqhosha. Umbala obomvu-oluhlaza okwesibhakabhaka kufuneka ubonakale emva kokugqiba inkqubo yempumelelo yovavanyo.

Ukugcinwa

1. Gcina ikhiti phantsi kwefriji eqhelekileyo (2 ~ 8 ℃).

Musa ukukhulula ikhiti.

2. Ikhithi iqulathe impahla yemvelo engasebenziyo. Ikhithi kufuneka ihlawulwe

kwaye walahlwa ngokuhambelana neemfuno zendawo yokuhlala yendawo.

Inkqubo yovavanyo

Ukulungiselela ngaphambi kokwenza uvavanyo:

1. Yiza ne-cartridge kubushushu begumbi (20 ℃ -30 ℃) kwaye ubeke kwibhentshi yomsebenzi de ilebhile ye-thermal i-thermal eludongeni iba ngumbala obomvu.

2. Iphepha lezicubu ezicocekileyo kwibhentshi yomsebenzi yokubeka isitshixo.

3. IPhix yokulahla inkunkuma ye-10μ kunye neengcebiso ezikwi-10μl yomgangatho womsebenzi.

4. Susa i-Aluminium ye-Aluminium ye-Aluminum kwaye iphose isitshixo sendlela esezantsi ye-cartridge kwiPhepha leZicubu ezicocekileyo.

5. Yima ngokuthe tye i-carchge kwibhentshi yomsebenzi kwaye uqinisekise ukuba amanani aphezulu ecandelo anokubonwa kwicala elifanelekileyo (izitampu zenani elichanekileyo. Cofa i-cartridge kancinci ukuze uqiniseke

Izisombululo kwiindawo eziphezulu zibuyela ezantsi.

Ukwenza uvavanyo:

I-1.CunCover ye-foil ekhuselayo kwiigumbi eziphezulu ngononophelo ngononophelo kunye nesithupha esivela ngasekhohlo uye ngasekunene kude kube kuphela kwicandelo le-1.

2.Uyifumene isampulu yegazi evavanyiweyo kunye ne-respenser esetwa usebenzisa i-piperte ye-pipette.

Kwi-serum yokuvavanya i-serum okanye i-plasma yokusetyenziswa kwe-5μl.

Ukuvavanya yonke igazi legazi le-10μl.

I-EDTA okanye i-heparin anticouhlant iyacetyiswa kwiPlasma kunye nokuqokelelwa kwegazi.

3. Idipozithi isampulu kwicandelo le-1. Emva koko phakamisa kwaye uphakamise amatyeli aliqela elininzi ekufezekiseni ukuxhuzula (isisombululo esiluhlaza okwesibhakabhaka kwincam ibonisa idiphozithi eyimpumelelo).

4.Pika isitshixo seqhosha leqhosha le-Forefinger kunye nesithupha ngocofileni kwaye ufake isitshixo seli cala liphambili lokujongana ne-1 Wena). Emva koko xuba kwaye ume isitshixo kwicandelo le-1 kwimizuzu emi-5.

5. Vumela i-foil yokukhusela ngokuqhubekayo ukuya ngasekunene kude kube kuphela kwicandelo 2. Thatha isitshixo ngumnini wecandelo le-2. Emva koko xuba kwaye ume isitshixo kwi

Igumbi eliphezulu 2 kumzuzu omnye.

I-6. Ukutyhila ububi obukhuselekileyo ngokuqhubekayo ukuya ngasekunene kude kube kuphela kwicandelo le-3. Thatha isitshixo ngumnini wecandelo le-3. Emva koko xuba iqhosha kwi

Iqela 3 kangangemizuzu emi-5.

7. Isiko lokukhusela ngokuqhubekayo ukuya ngasekunene kude kube kuphela kwicandelo le-4. Emva koko faka iqhosha kwicandelo le-4 eliphezulu.

8. I-FOUNONIVE ye-foil ekhuselayo ngokuqhubekayo ukuya ngasekunene kude kube kuphela kwicandelo 5. Thatha isitshixo ngumnini wecandelo le-5. Emva koko faka isitshixo kwicandelo eliphezulu 5 kumzuzu omnye.

I-FOIL ekhuselayo ngokuqhubekayo ngasekunene kude kube kuphela kwicandelo le-6. Thatha isitshixo kwicandelo lecandelo le-6 kwimizuzu emi-5 kangangemizuzu emi-6.

I-FOIL ekhuselayo ngokuqhubekayo ngasekunene kude kufike kuphela kwicandelo le-7. Emva koko faka isitshixo kwicandelo lecandelo eliphezulu 7 kumzuzu omnye.

11. Thatha isitshixo seli candelo lesi-7 kwaye uyeke iphepha lezicubu kangangemizuzu emi-5 ngaphambi kokufunda iziphumo.

 Amanqaku:

Sukuchukumisa i-FREATE yecala elingaphambili lesitshixo, apho iiproteni kunye neproteni yolawulo zifakwa (uvavanyo kunye nengingqi yolawulo).

Kunqande ukukrala uvavanyo kunye nommandla wokulawula ngokuncamathela elinye icala eligudileyo lesiphelo selindo kwicandelo langaphakathi kwigumbi ngalinye eliphezulu ngelixa uxuba.

Ukuxuba, amaxesha ali-10 okukhulisa kunye nokuthoba isitshixo kwigumbi ngalinye eliphezulu kuyacetyiswa.

Ichaza kuphela igumbi elinye elilandelayo ngaphambi kokudlulisela isitshixo.

Ukuba kukho imfuneko, qhoboshela iilebheli ezinikezelweyo zezilwanyana ezininzi ngaphezulu kovavanyo lwesampulu enye.

Iziphumo zovavanyo

Jonga amabala akhutshekileyo kwiqhosha lemibala esemgangathweni

Ayisebenzi:

Akukho mbala ubonakalayo-eluhlaza okwesibhakabhaka ivela kwindawo yolawulo

Engalunganga(-)

Akukho mbala ubonakalayo-luhlaza okwesibhakabhaka ivela kwiindawo zovavanyo

(+)

Umbala obomvu-oluhlaza okwesifuba uvela kwiindawo zovavanyo

Iitati zee-antibodies ezithile ze-IGG zinokuboniswa ngamanqanaba amathathu