I-FPLVFHVFCV Ikhithi yovavanyo ye-IgG

I-FELINE PANLEUKOPENIA/HERPES VIRUS/CALICI VIRUS IgG ANTIBODY TEST KIT (ikhithi yovavanyo ye-FPLV/FHV/FCV IgG) yenzelwe ukuvavanya ngokobungakanani bekati i-IgG antibody amanqanaba e-Feline Panleunia (FPLV), Feline Herpes Virus kunye neFeline Herpes Virus ( Intsholongwane (FCV).

IZIQULATHO ZEKHITHI

UYILO NOMGAQO

Kukho amacandelo amabini afakwe kwi-cartridge nganye: Isitshixo, esifakwe kunye ne-desiccant kwindawo ephantsi etywinwe nge-aluminium ekhuselayo, kunye nokuphuhlisa izisombululo, ezifakwe ngokwahlukileyo kwiindawo eziphezulu ezifakwe kwi-aluminium foil ekhuselayo. Ikhatriji nganye iqulethe zonke ii-reagents eziyimfuneko kwisampula enye yovavanyo. Ngokufutshane, xa iSitshixo sifakwe kwaye sifakwe kwimizuzu embalwa kwi-compartment ephezulu ye-1, apho isampuli yegazi ifakwe khona, i-antibodies ethile ye-IgG kwisampuli yegazi ehlanjululweyo, ukuba ikhona, iya kubophelela kwi-FPLV, FHV okanye Ii-antigens ze-FCV eziphinda-phindeneyo zingashukumi ngokwahlukileyo

iindawo ezidityanisiweyo kwiSitshixo esifakiweyo. Emva koko iSitshixo siya kudluliselwa kumacandelo aphezulu aseleyo ngamaxesha amisiweyo inyathelo ngenyathelo. I-antibodies ye-IgG ebophelelweyo kumabala iya kubhalwa kwi-compartment ephezulu ye-3, equlethe i-anti-feline IgG enzyme conjugate kunye neziphumo zokugqibela ezivezwe njengamabala amfusa-blue ziya kuphuhliswa kwi-compartment 6 ephezulu, equlethe i-substrate. Ukufumana isiphumo esanelisayo, amanyathelo okuhlamba aziswa. Kwi-compartment ephezulu ye-2, i-IgG engapheliyo kunye nezinye izinto ngaphakathi kwesampuli yegazi ziya kususwa. Kwi-compartment ephezulu ye-4 kunye ne-5, i-anti-feline ye-IgG ye-enzyme ye-conjugate engavumelekanga okanye engaphezulu iya kupheliswa ngokwaneleyo. Ekugqibeleni, kwi-compartment ephezulu ye-7, i-chromosome engaphezulu iphuhliswe kwi-substrate kunye ne-enzyme edibeneyo i-conjugate kwi-compartment ephezulu ye-6 iya kususwa.

Ukuqinisekisa ukunyaniseka kwentsebenzo, iprotheni yokulawula ifakwe kwindawo ephezulu kakhulu kwiSitshixo. Umbala omfusa-blue kufuneka ubonakale emva kokugqiba inkqubo yokuvavanya ngempumelelo.

UKUGCINA

1. Gcina ikiti phantsi kwefriji eqhelekileyo (2 ~ 8℃).

MUSA UKUYIMKHETHISA IKHITHI.

2. Ikhithi iqulethe imathiriyeli yebhayoloji engasebenziyo. Ikhithi kufuneka iphathwe

kwaye ilahlwe ngokuhambelana neemfuno zococeko lwasekhaya.

INKQUBO YOVAVANYO

Ukulungiselela phambi kokwenza uvavanyo:

1. Zisa i-cartridge kwiqondo lokushisa lokushisa (20℃-30℃) kwaye uyibeke kwibhentshi yomsebenzi de i-label ye-thermal eludongeni lwe-cartridge ibe ngumbala obomvu.

2.Beka i-tissue paper ecocekileyo kwibhentshi yomsebenzi yokubeka iSitshixo.

3.Lungisa i-10μL yokukhupha kunye ne-10μL iingcebiso eziqhelekileyo zepayipi.

4. Susa ifoyile ye-aluminiyam ekhuselayo esezantsi kwaye uphose iSitshixo ngaphandle kwecandelo elisezantsi lekhatriji kwiphepha lethishu elicocekileyo.

5. Yima nkqo ikhatriji kwibhentshi yomsebenzi kwaye uqinisekise ukuba amanani ekhompatimenti aphezulu angabonwa kwicala elichanekileyo (izitampu zamanani ezichanekileyo zijonge kuwe). Cofa kancinci kwikhatriji ukuze uqiniseke ukuba

izisombululo kumacandelo aphezulu zijika zibuyele ezantsi.

Ukwenza uvavanyo:

1.Vula ifoyile ekhuselayo kumagumbi aphezulu ngocoselelo ngomnwe ongaphambili kunye nobhontsi ukusuka ekhohlo ukuya ekunene de kuvele KUPHELA indawo ephezulu yoku-1.

2. Fumana isampuli yegazi evavanyiweyo kunye nesethi ye-dispenser usebenzisa i-tip ye-pipette ye-10μL eqhelekileyo.

Ukuvavanya i-serum okanye i-plasma sebenzisa i-5μL.

Ukuvavanya sebenzisa igazi elipheleleyo i-10μL.

I-EDTA okanye i-heparin anticoagulant tubes iyacetyiswa kwi-plasma kunye nokuqokelela kwegazi lonke.

3. Beka isampuli kwi-compartment ephezulu 1. Emva koko uphakamise kwaye unciphise i-dispenser plunger amaxesha amaninzi ukuphumeza ukuxuba (Isisombululo esiluhlaza okwesibhakabhaka kwincam xa ukuxuba kubonisa idiphozithi eyimpumelelo).

4.Chola iSitshixo ngesiBambi seSitshixo ngomnwe ongaphambili kunye nobhontsi ngononophelo kwaye ufake isitshixo kwindawo ephezulu 1 (qinisekisa icala leqabaka leSitshixo elijongene nawe, okanye qinisekisa ukuba isangqa esikwisiBambi sisekunene xa ujongene nalo. wena). Emva koko xuba kwaye ume iSitshixo kwindawo ephezulu ye-1 yemizuzu emi-5.

5. Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de kube KUPHELA uveza indawo 2. Chola isitshixo ngesiBambi uze ufake isitshixo kwindawo evulekileyo 2. Emva koko xuba kwaye ume iSitshixo kwindawo

indawo ephezulu 2 ye-1 umzuzu.

6. Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de kube KUPHELA ukuveza ikhompatimenti 3. Chola isitshixo ngesiBambi uze ufake isitshixo kwindawo evulekileyo 3. Emva koko xuba kwaye ume iSitshixo kwindawo

indawo 3 imizuzu emi-5.

7.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene kude KUPHELA ukuveza i-compartment 4. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 4. Emva koko udibanise kwaye umise iSitshixo kwindawo ephezulu ye-4 kwi-1 ngomzuzu.

8.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 5. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 5. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu ye-5 kwi-1 ngomzuzu.

9.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 6. Chola iSitshixo ngesiBambi kwaye ufake isitshixo kwindawo evulekileyo 6. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu yesi-6 imizuzu emi-5.

10.Vula ifoyile ekhuselayo ngokuqhubekayo ukuya ngasekunene de uveze KUPHELA igumbi 7. Thatha iSitshixo nguMphathi kwaye ufake iSitshixo kwindawo evulekileyo 7. Emva koko xuba kwaye umise iSitshixo kwindawo ephezulu yesi-7 kwi-1 ngomzuzu.

11. Khupha iSitshixo kwigumbi eliphezulu lesi-7 kwaye uyomise kwiphepha lethishu malunga nemizuzu emi-5 phambi kokuba ufunde iziphumo.

 Amanqaku:

Musa ukuthintela i-Frosting Side ye-Frosting Front of the Key, apho i-antigens kunye neprotheni yokulawula ingenakunyakaziswa (Uvavanyo kunye noLawulo lweNgingqi).

Kuphephe ukukrwela ukukrwela uVavanyo kunye noLawulo loMmandla ngokungqiyama elinye icala eliSmooth lesiphelo sangaphambili seSitshixo ukuya eludongeni lwangaphakathi lwekhompatimenti nganye ephezulu ngelixa udibanisa.

Ukuxuba, amaxesha e-10 aphakamisa kunye nokunciphisa iSitshixo kwindawo nganye ephezulu iyanconywa.

Veza kuphela indawo ephezulu elandelayo phambi kokudlulisela isitshixo.

Ukuba kukho imfuneko, ncamathele iiLeyibhile zeSilo esibonelelweyo kwiimvavanyo ezingaphezulu kwesinye.

UKUTOlika IZIPHUMO ZOKUVAVANYA

Jonga amabala anesiphumo kwiSitshixo ngoMbala woMbala osemgangathweni

Ayisebenzi:

AKUKHO mbala umfusa-blue ubonakala kwindawo yolawulo

Ibi(-)

AKUKHO mbala umfusa-blue ubonakalayo kwiindawo zovavanyo

Okuhle (+)

Umbala omfusa-blue obonakalayo uyavela kwiindawo zovavanyo

I-titters ye-antibodies ye-IgG ethile inokubonakaliswa ngamanqanaba amathathu