【UKUSETYENZISWA OKUHLOSIWEYO】

Testsealabs®COVID-19 IgG/IgM Antibody Test Cassette yi-lateral flow chromatographic immunoassay ukuze kufunyanwe umgangatho we-IgG kunye ne-IgM antibodies kwi-COVID-19 kwigazi lomntu lonke, i-serum okanye isampuli yeplasma.

【Inkcazo】

I-20pc/ibhokisi (izixhobo zovavanyo ezingama-20+ iityhubhu ezingama-20+1buffer+1 faka imveliso)

【IZIXHOBO EZINIKEZELWAYO】

1.Izixhobo zovavanyo
2.Isithinteli
3.Iidropha
4.Ukufakwa kweMveliso

【UKUQOKELELWA KWEENKCUKACHA】

I-SARS-CoV2 (COVID-19) ye-IgG/IgM Antibody Test Cassette (Igazi Elipheleleyo/iSerum/ iPlasma) inokwenziwa kusetyenziswa igazi elinomngxuma (ukusuka kwi-venipuncture okanye intonga yeminwe), i-serum okanye iplasma.

1.Ukuqokelela iFingerstick Whole Blood Samples:
2.Hlamba isandla sesigulana ngesepha kunye namanzi ashushu okanye ucoce nge-alcohol swab. Vumela ukuba zome.
3.Hlikihla isandla ngaphandle kokuchukumisa indawo yokuhlatywa ngokuhlikihla isandla ukuya kwincam yomnwe wombindi okanye weringi.
4.Hlaba ulusu nge-lancet enyumba. Sula uphawu lokuqala lwegazi.
5. Hlikihla ngobunono isandla ukusuka esihlahleni ukuya entendeni ukuya emnweni ukwenza ithontsi legazi elingqukuva kwindawo yokuhlatywa.
6.Yongeza i-Fingerstick Whole Blood specimen kuvavanyo ngokusebenzisa ityhubhu ye-capillary:
7.Bamba isiphelo setyhubhu ye-capillary egazini de uzaliswe malunga ne-10mL. Waphephe amaqamza omoya.
8.Ukwahlula i-serum okanye i-plasma egazini ngokukhawuleza kunokwenzeka ukuphepha i-hemolysis. Sebenzisa kuphela iisampulu ezicacileyo ezingezizo hemolyzed.

【INDLELA YOKUVAVANYA】

Vumela uvavanyo, umfuziselo, isithinteli kunye/okanye nolawulo ukufikelela kubushushu begumbi (15-30°C) phambi kovavanyo.

Susa ikhasethi yovavanyo kwisingxobo sefoil kwaye uyisebenzise kwiyure enye. Iziphumo ezingcono kakhulu ziya kufumaneka ukuba uvavanyo lwenziwa ngokukhawuleza emva kokuvula isikhwama se-foil.
Beka ikhasethi kwindawo ecocekileyo kunye nenqanaba. NgeSerum okanye isampuli yePlasma:

• Ukusebenzisa i-dropper: Bamba i-dropper ngokuthe nkqo, zoba isampuli kumgca wokuzalisa (malunga ne-10mL), kwaye udlulisele umboniso kwi-specimen kakuhle (S), uze udibanise ama-2 amathontsi e-buffer (malunga ne-80 mL), kwaye uqalise isibali-xesha. .
• Ukusebenzisa i-pipette: Ukudlulisa i-10 mL yomzekelo kwiqula le-specimen(S), emva koko wongeze amathontsi ama-2 e-buffer (malunga ne-80 mL), kwaye uqalise isibali-xesha.

KwiVenipuncture yeGazi elipheleleyo:

• Ukusebenzisa i-dropper: Bamba i-dropper ngokuthe nkqo, zoba i-specimen malunga ne-1 cm ngaphezu komgca wokuzalisa kwaye udlulisele i-drop e-1 epheleleyo (malunga ne-10μL) yesampuli kwiqula lesampuli (S). Emva koko yongeza amathontsi ama-2 e-buffer (malunga ne-80 mL) kwaye uqalise isibali-xesha.
• Ukusebenzisa i-pipette: Ukudlulisela i-10 mL yegazi elipheleleyo kwi-specimen yequla (S), uze udibanise ama-2 amathontsi e-buffer (malunga ne-80 mL), kwaye uqalise isibali-xesha.
• Ngomnwe weGazi elipheleleyo:
• Ukusebenzisa i-dropper: Bamba i-dropper ngokuthe nkqo, zoba i-specimen malunga ne-1 cm ngaphezu komgca wokuzalisa kwaye udlulisele i-drop e-1 epheleleyo (malunga ne-10μL) yesampuli kwiqula lesampuli (S). Emva koko yongeza amathontsi ama-2 e-buffer (malunga ne-80 mL) kwaye uqalise isibali-xesha.
• Ukusebenzisa ityhubhu ye-capillary: Gcwalisa ityhubhu ye-capillary kwaye ugqithisele malunga ne-10mL ye-fingerstick yegazi elipheleleyo kwi-specimen yequla (S) lekhasethi yovavanyo, uze udibanise ama-2 amathontsi e-buffer (malunga nama-80 mL) kwaye uqalise isibali-xesha. Jonga umfanekiso ongezantsi.
• Lindela ukuba umgca onemibala ibonakale. Funda iziphumo ngemizuzu eyi-15. Musa ukutolika umphumo emva kwemizuzu engama-20.
• Qaphela: Kucetyiswa ukuba ungasebenzisi isithinteli, ngaphaya kweenyanga ezi-6 emva kokuvula i-vial.

【UKUTOLIKWA KWEZIPHUMO】

I-IgG POSITIVE:* Imigca emibini enemibala iyavela. Umgca omnye onemibala kufuneka uhlale ubonakala kwingingqi yolawulo (C) kwaye omnye umgca kufuneka ube kwindawo yomgca we-IgG.

I-IgM OKUHLE:* Kuvela imigca emibini enemibala. Umgca omnye onemibala kufuneka uhlale uvela kummandla wolawulo (C) kwaye omnye umgca kufuneka ube kwindawo yomgca we-IgM.

I-IgG kunye ne-IgM POSITIVE:* Imigca emithathu enemibala iyavela. Umgca omnye onemibala kufuneka uhlale uvela kummandla wolawulo (C) kunye nemigca emibini yokuvavanya kufuneka ibe kwindawo ye-IgG kunye ne-IgM lineregion.

*QAPHELA: Ubunzulu bombala kwiindawo zovavanyo bunokwahluka ngokuxhomekeke kuxinaniso lwezilwa-buhlungu ze-COVID-19 ezikhoyo kumzekelo. Ngoko ke, nawuphi na umthunzi wombala kwingingqi yomgca wovavanyo kufuneka uthathwe njengento enhle.

I-NEGATIVE: Umgca omnye onemibala ubonakala kwindawo yokulawula umgca (C). Akukho mgca uvela kummandla we-IgG kunye nommandla we-IgM.

AYIFANELEKILEYO: Umgca wolawulo awuphumeleli ukuvela. Umthamo wesampulu engonelanga okanye ubuchule benkqubo obungachanekanga zezona zizathu ezinokuthi zibe kho zokungaphumeleli komgca wolawulo. Ukuphonononga inkqubo uvavanyo ngovavanyo olutsha. Ukuba ingxaki iyaqhubeka, yeka ukusebenzisa ikiti yovavanyo ngoko nangoko kwaye uqhagamshelane nomsasazi wakho wendawo.