SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)
【TŠEBELETSO TŠEBELETSO】
SARS-CoV-2 Neutralizing Antibody Detection Kit ke Competitive Enzyme-Linked Immunosorbent Assay (ELISA) e reretsoeng ho sibolloa ha boleng le ho lekana ha li-antibodies ho SARS-CoV-2 ho serum ea motho le plasma. SARS-CoV-2 Neutralizing Antibody Detection Kit e ka sebelisoa e le thuso ho tsebahatseng batho ba nang le karabelo ea 'mele ea ho itšireletsa mafung ho SARS-CoV-2, e bonts'ang ts'oaetso ea morao-rao kapa ea pele. SARS-CoV-2 Neutralizing Antibody Detection Kit ha ea lokela ho sebelisoa ho fumana tšoaetso e matla ea SARS-CoV-2.
【LIEKETSENG】
Ts'oaetso ea Coronavirus hangata e baka karabelo ea li-antibody tse fokolang. Litefiso tsa seroconversion ho bakuli ba COVID-19 ke 50% le 100% ka letsatsi la 7 le la 14 ho qala ha matšoao, ka ho latellana. Ho fana ka tsebo, kokoana e tsamaellanang e fokotsang sesole sa 'mele maling e nkoa e le sepheo sa ho tseba ho sebetsa hantle ha li-antibody 'me khatello e phahameng ea sesole sa 'mele e bonts'a ts'ireletso e phahameng. Teko ea Phokotso ea Phokotso ea ho Neutralization (PRNT) e ntse e nkoa e le tekanyetso ea khauta ea ho lemoha li-antibodies tse fokolang. Leha ho le joalo, ka lebaka la tlhahiso ea eona e tlase le tlhoko e phahameng ea ts'ebetso, PRNT ha e sebetse bakeng sa tlhahlobo e kholo ea serodiagnosis le tlhahlobo ea ente. SARS-CoV-2 Neutralizing Antibody Detection Kit e ipapisitse le mokhoa oa Competitive Enzyme-Linked Immunosorbent Assay (ELISA), o ka lemohang antibody e sa nke lehlakore sampoleng ea mali le ho fihlella ka ho khetheha maemo a mahloriso a mofuta ona oa antibody.
【Mokhoa oa Teko】
1.Ka li-tubes tse arohaneng, aliquot 120μL ea Tharollo e lokiselitsoeng ea hACE2-HRP.
2.Eketsa 6 μL ea li-calibrator, lisampole tse sa tsejoeng, taolo ea boleng ka har'a tube e 'ngoe le e' ngoe 'me u kopanye hantle.
3.Fetisetsa 100μL ea motsoako o mong le o mong o lokiselitsoeng mohatong oa 2 ho liliba tsa microplate tse tsamaellanang ho latela tlhahlobo e reriloeng esale pele.
3. Koahela poleiti ka Plate Sealer 'me u e kene ka 37°C metsotso e 60.
4.Tlosa Plate Sealer 'me u hlatsoe poleiti ka hoo e ka bang 300 μL ea 1× Hlatsoa Tharollo selibeng ka makhetlo a mane.
5. Tlanya poleiti holim'a thaole ea pampiri ho tlosa mokelikeli o setseng ka lilibeng ka mor'a ho hlatsoa mehato.
6. Kenya 100 μL ea Tharollo ea TMB selibeng ka seng 'me u etsetse poleiti lefifing ka 20 - 25°C metsotso e 20.
7.Eketsa 50 μL ea Stop Solution selibeng ka seng ho emisa karabelo.
8.Bala ho monya ho sebali sa microplate ho 450 nm nakong ea metsotso ea 10 (630nm e le sesebelisoa se khothalletsoang bakeng sa ts'ebetso e nepahetseng e phahameng.
