OEM Manufacturer China Medomics Infectious COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist

Hona joale re na le basebetsi ba bangata ba hloahloa ba ipabolang ka ho bapatsa marang-rang, QC, 'me ba sebetsa ka mefuta ea mathata a thata ka har'a mokhoa oa tlhahiso bakeng sa Moetsi oa OEM China Medomics Infectious COVING Virus Neutralization Antibody Testing Kit W/CE Mark & ​​Whitelist, Re bile ba bang. ea lihlahisoa tsa hau tse kholo ka ho fetisisa tsa 100% Chaena.Likhoebo tse ngata tse kholo tsa khoebo li kenya lihlahisoa le litharollo ho tsoa ho rona, kahoo re ka u fa theko ea theko e ntle ka ho fetisisa e nang le boleng bo tšoanang ho mang kapa mang ea re thahasellang.
Hona joale re na le basebetsi ba bangata ba hloahloa ba ipabolang ka ho bapatsa marang-rang, QC, le ho sebetsa le mefuta ea mathata a thata ka har'a mokhoa oa ho etsa thepa.Feme ea Antibody Neutralizing, Moetsi oa Antibody Neutralizing, Antibody Neutralizing mofani oa thepa, Teko ea Antibody Neutralizing, China Medical, Antibody e Neutraling, Hore na o khetha sehlahisoa sa hajoale lethathamong la rona kapa o batla thuso ea boenjiniere bakeng sa kopo ea hau, o ka bua le setsi sa rona sa litšebeletso tsa bareki mabapi le litlhoko tsa hau tsa ho fumana.Re ka fana ka boleng bo botle ka theko ea tlholisano bakeng sa hau ka bouena.

【MOLAOMOTHEO】

SARS-CoV-2Antibody e NeutralingDetection Kit e ipapisitse le mokhoa oa tlholisano oa ELISA.

Ho sebelisa purified receptor binding domain (RBD), protheine e tsoang ho viral spike (S) protheine le sele e amohelang.

receptor ACE2, tlhahlobo ena e etselitsoe ho etsisa tšebelisano ea kokoana-hloko ea ho se nke lehlakore.

Li-Calibrator, Litaolo tsa Boleng, le lisampole tsa serum kapa plasma li tsoakoa ka bonngoe hantle ka ho hlapolloa

buffer e nang le haACE2-HRP conjugate e qotsitsoeng ka li-tubes tse nyane.Ebe metsoako e fetisetsoa ka hare

liliba tsa microplate tse nang le sekhechana sa SARS-CoV-2 RBD (RBD) sa immobilized recombinant.

ho qhomisa.Nakong ea ho futhumala ha metsotso e 30, antibody e khethehileng ea RBD ka har'a li-calibrators, QC le

lisampole li tla qothisana lehlokoa le haACE2-HRP bakeng sa ho tlama RBD e sa tsamaeeng lilibeng.Kamora

ho qhoqhoa, liliba li hlatsuoa ka makhetlo a 4 ho tlosa conjugate e sa tlamisoang ea haACE2-HRP.Tharollo ea

Joale TMB e ea eketsoa le ho kenngoa metsotso e 20 mocheso oa kamore, e leng se etsang hore ho be le tsoelo-pele ea

mmala o moputsoa.Tsoelo-pele ea 'mala e emisoa ka ho eketsoa ha 1N HCl,' me monyetla o

e lekantsoe spectrophotometrically ho 450 nm.Matla a 'mala o entsoeng a lekana le

palo ea enzyme e teng, 'me e amana ka tsela e fapaneng le palo ea litekanyetso tse lekantsoeng ka mokhoa o ts'oanang.

Ka ho bapisa le lekhalo la calibration le entsoeng ke li-calibrator tse fanoeng, mahloriso a

Li-antibodies ho sampole tse sa tsejoeng lia baloa.

【LITLHAKISO TSE HLOKAHANG EMPA HA LI ABONE】

1. Metsi a hloekisitsoeng kapa a hloekisitsoeng

2. Lipeipi tse nepahetseng: 10μL, 100μL, 200μL le 1 mL

3. Litlhahiso tsa pipette tse lahliloeng

4. Sebali sa Microplate se khonang ho bala monyetla ho 450nm.

5. Pampiri e monyang

6. Pampiri ea kerafo

7. Vortex mixer kapa e lekanang

【POKETSO LE POLOKO】

1. Lisampole tsa Serum le Plasma tse bokeletsoeng ka har'a li-tubes tse nang le K2-EDTA li ka sebelisoa bakeng sa khithi ena.

2. Mehlala e lokela ho koaheloa 'me e ka bolokoa ho fihlela lihora tse 48 ho 2 °C - 8 °C pele ho tekolo.

Mehlala e tšoaretsoeng nako e telele (ho fihlela likhoeling tse 6) e lokela ho hoamisoa hanngoe feela ho -20 °C pele ho tlhahlobo.

Qoba makhetlo a mangata a ho qhibiliha a leqhoa.

TS'ELISO

【Reagent Tokiso】

1. Li-reagents tsohle li tlameha ho ntšoa ka sehatsetsing ebe li lumelloa ho khutlela mocheso oa kamore pele li sebelisoa

(20° ho isa ho 25°C).Boloka li-reagents tsohle ka sehatsetsing hang ka mor'a hore u li sebelise.

2. Lisampole tsohle le litsamaiso li lokela ho ba vortexed pele li sebelisoa.

3. hACE2-HRP Tokiso ea Tharollo: Hlakola hACE2-HRP concentrate ho 1: 51 dilution ratio with Dilution

Buffer.Mohlala, hlapolla 100 μL ea haACE2-HRP e tsepamisitsoeng maikutlo ka 5.0mL ea HRP Dilution Buffer ho

etsa tharollo ea haACE2-HRP.

4. 1× Tokiso ea Tharollo ea Hlatsoa: Hlatsoa Tharollo ea 20× Hlatsoa ka metsi a hloekisitsoeng kapa a qhibilihisitsoeng ka metsi.

karo-karolelano ea bophahamo ba 1:19.Mohlala, hlapolla 20 mL ea 20× Hlatsoa Tharollo ka 380 mL ea deionized kapa

metsi a halikiloeng ho etsa 400 mL ea 1× Hlatsoa Tharollo.

【Mokhoa oa Teko】

1. Ka li-tubes tse arohaneng, aliquot 120μL ea Tharollo e lokiselitsoeng ea hACE2-HRP.

2. Eketsa 6 μL ea li-calibrator, lisampole tse sa tsejoeng, lisebelisoa tsa boleng ka har'a tube e 'ngoe le e' ngoe 'me u kopanye hantle.

3. Fetisetsa 100μL ea motsoako o mong le o mong o lokisitsoeng mohatong oa 2 ho liliba tse tsamaellanang tsa microplate ho latela

ho tlhophiso e reriloeng esale pele ea tlhahlobo.

3. Koahela poleiti ka Plate Sealer 'me u e kene ka 37°C metsotso e 30.

4. Tlosa Plate Sealer 'me u hlatsoe poleiti ka hoo e ka bang 300 μL ea 1× Hlatsoa Tharollo selibeng ka makhetlo a mane.

5. Tlanya poleiti holim'a thaole ea pampiri ho tlosa mokelikeli o setseng ka lilibeng ka mor'a ho hlatsoa mehato.

6. Eketsa 100 μL ea Tharollo ea TMB selibeng ka seng 'me u etsetse poleiti lefifing ka 20 - 25°C metsotso e 20.

7. Kenya 50 μL ea Stop Solution selibeng ka seng ho emisa karabelo.

8. Bala ho monyela ha sebali sa microplate ho 450 nm nakong ea metsotso e 10 (630nm joalo ka ha ho le joalo).

kgothaletswa bakeng sa tshebetso e nepahetseng e phahameng).