SARS-CoV-2 RT-PCR Detection Kit ea nako ea 'nete

Setsi sena se reretsoe ho lemoha boleng ba in vitro ba liphatsa tsa lefutso tsa ORF1ab le N ho tsoa ho 2019-nCoV ka pharyngeal swab kapa bronchoalveolar lavage specimens tse bokelletsoeng ho tsoa ho linyeoe tsa Coronavirus 2019 (COVID-19) tse belaelloang, lihlopha tse belaelloang tsa linyeoe, kapa batho ba bang ba hlokang 2019. - tlhahlobo ea tšoaetso ea nCoV kapa tlhahlobo ea phapang.

Kit e etselitsoe ho lemoha RNA ea 2019-nCoV ka mehlala e sebelisang theknoloji ea RTPCR ea nako ea sebele ea multiplex le libaka tse sirelelitsoeng tsa liphatsa tsa lefutso tsa ORF1ab le N e le libaka tse hlakotsoeng tsa li-primers le probes. Ka nako e ts'oanang, setsi sena se na le mokhoa oa ho lemoha oa endogenous control (The gene ea control e ngotsoe ke Cy5) ho shebella mokhoa oa ho bokella mohlala, nucleic acid extract le PCR le ho fokotsa liphello tse fosahetseng tsa bohata.

Likarolo tsa Bohlokoa:

1. Katoloso e potlakileng, e ka tšeptjoang le kenyeletso ea ho lemoha : SARS joalo ka coronavirus le tlhahlobo e khethehileng ea SARS-CoV-2

2. Mohato o le mong oa RT-PCR reagent (pofo ea lyophilized)

3. E kenyelletsa taolo e ntle le e mpe

4. Lipalangoang ka mocheso o tloaelehileng

5. Thepa e ka lula e tsitsitse ho fihlela likhoeling tse 18 e bolokiloe ho -20℃.

6. CE e amohetsoe

Phallo :

1. Lokisetsa RNA e ntšitsoeng ho SARS-CoV-2

2. Fokotsa taolo e ntle ea RNA ka metsi

3. Lokisetsa PCR master mix

4. Kenya motsoako o moholo oa PCR le RNA ka har'a poleiti ea nako ea sebele ea PCR kapa tube

5. Sebelisa sesebelisoa sa PCR sa nako ea sebele