Monkeypox Virus (MPV) Nucleic Acid Detection Kit
LIEKETSENG
Thepa e sebelisoa bakeng sa tlhahlobo ea in vitro ea maemo a belaelloang a Monkeypox Virus (MPV), linyeoe tse kopaneng le linyeoe tse ling tse hlokang ho fumanoa bakeng sa tšoaetso ea Monkeypox Virus.
Thepa e sebelisoa ho lemoha mofuta oa f3L oa MPV ka har'a 'metso le lisampole tsa nasal swab.
Liphetho tsa tlhahlobo ea khithi ena ke tsa litšupiso tsa bongaka feela 'me ha lia lokela ho sebelisoa e le eona feela tekanyetso ea tlhahlobo ea bongaka. Ho kgothaletswa ho etsa tlhahlobo e pharaletseng ea boemo bo thehiloeng ho kliniki ea mokuli
lipontšo le liteko tse ling tsa laboratori.
Tšebeliso e reriloeng
| Mofuta oa tlhahlobo | memetso le mamina a nko |
| Mofuta oa teko | Boleng ba boleng |
| Boitsebiso ba teko | PCR |
| Pakete ea boholo | Liteko tse 48/1 lebokose |
| Mocheso oa polokelo | 2-30 ℃ |
| Shelf bophelo | Likhoeli tse 10 |
SEHLAHLISO SEHLOOHO
Molao-motheo
Setsi sena se nka tatellano e bolokehileng ea mofuta oa MPV f3L joalo ka sebaka seo ho shebiloeng ho sona. Theknoloji ea nako ea 'nete ea fluorescence quantitative PCR le thekenoloji ea ho lokolloa ka potlako ea nucleic acid e sebelisoa ho lekola viral nucleic acid ka phetoho ea lets'oao la fluorescence la lihlahisoa tsa amplification. Mokhoa oa ho lemoha o kenyelletsa taolo ea boleng ba ka hare, e sebelisetsoang ho hlahloba hore na ho na le PCR inhibitors ka lisampole kapa hore na lisele tsa lisampole li nkiloe, tse ka thibelang ka katleho boemo bo bobe ba bohata.
LIEKETSENG MAHLOKO
Kit e na le li-reagents bakeng sa ho sebetsana le liteko tse 48 kapa taolo ea boleng, ho kenyelletsa le likarolo tse latelang:
Reagent A
| Lebitso | Likarolo tse ka sehloohong | Bongata |
| Ho fumana MPV reagent | The reaction tube e na le Mg2+, f3L gene / Rnase P primer probe, buffer reaction, Taq DNA enzyme. | 48 liteko |
ReagentB
| Lebitso | Likarolo tse ka sehloohong | Bongata |
| MPV Taolo e Ntle | E na le sekhechana sa sepheo sa MPV | 1 tube |
| MPV Taolo e Fosahetseng | Ntle le sekhechana sa sepheo sa MPV | 1 tube |
| DNA e lokollang reagent | Reagent e na le Tris, EDTA le Triton. | 48pcs |
| Reconstitution reagent | DEPC metsi a hloekisitsoeng | 5ML |
Tlhokomeliso: Likarolo tsa linomoro tse fapaneng tsa batch li ke ke tsa sebelisoa ka ho fapanyetsana
【Maemo a polokelo le Bophelo ba Shelf】
1.Reagent A / B e ka bolokoa ho 2-30 ° C, 'me bophelo ba sethala ke likhoeli tse 10.
2.Ka kopo bula sekoahelo sa tube ea teko feela ha u se u itokiselitse tlhahlobo.
3.U se ke ua sebelisa li-tubes tsa teko ho feta letsatsi la ho fela.
4.U se ke ua sebelisa tube ea ho lemoha e lutlang.
【Seletsa se Sebetsang】
E loketse bakeng sa Sistimi ea tlhahlobo ea LC480 PCR, sistimi ea tlhahlobo ea Gentier 48E Automatic PCR, sistimi ea tlhahlobo ea ABI7500 PCR.
【Litlhoko tsa Mohlala】
1.Mefuta ea mehlala e sebetsang: lisampole tsa 'metso.
2.Sampling tharollo:Kamora ho netefatsoa, ho khothaletsoa ho sebelisa tšupa e tloaelehileng ea letsoai kapa ea ho boloka kokoana-hloko e hlahisoang ke Hangzhou Testsea biology bakeng sa ho bokella sampole.
mometso:Hlakola lithane tse peli tsa pharyngeal le lebota le ka morao la pharyngeal ka swab ea sampole e hloekisitsoeng, kenya sesepa ka har'a tube e nang le 3mL sampling solution, u lahle mohatla, 'me u tiise sekoaelo sa tube.
3.Sample polokelo le delibari:Mehlala e lokelang ho lekoa e lokela ho lekoa kapele kamoo ho ka khonehang. Mocheso oa lipalangoang o lokela ho bolokoa ho 2 ~ 8 ℃. Mehlala e ka hlahlojoang ka hare ho lihora tse 24 e ka bolokoa ho 2 ℃ ~ 8 ℃ 'me haeba lisampole li ke ke tsa lekoa ka hare ho lihora tse 24, li lokela ho bolokoa ka tlase ho kapa ho lekana. ho ea ho -70 ℃ (haeba ho se na boemo ba polokelo ea -70 ℃, e ka bolokoa ho -20 ℃ ka nakoana), qoba ho pheta-pheta
ho qhibidiha le ho qhibidiha.
4.Pokello e nepahetseng ea mohlala, polokelo, le lipalangoang ke tsa bohlokoa mosebetsing oa sehlahisoa sena.
【Mokhoa oa ho Leka】
1.Sample processing le ho eketsa mohlala
1.1 Mohlala oa ho sebetsa
Kamora ho kopanya tharollo ea sampole e kaholimo le lisampole, nka 30μL ea sampole ka har'a tube ea reagent ea DNA ebe u e kopanya ka ho lekana.
1.2 Ho kenya
Nka 20μL ea reagent ea reconstitution 'me u e kenye mochineng oa ho lemoha oa MPV, eketsa 5μL ea sampuli e entsoeng ka holimo (Taolo e ntle le taolo e mpe e tla sebetsoa ka ho tsamaisana le lisampole), koahela sekoahelo sa tube, se centrifuge ka 2000rpm bakeng sa 10. metsotsoana.
2. PCR amplification
2.1 Laola poleiti ea PCR e lokiselitsoeng ho sesebelisoa sa PCR sa fluorescence, Taolo e mpe le taolo e ntle e tla beoa bakeng sa tlhahlobo ka 'ngoe.
2.2 Tlhophiso ea kanale ea Fluorescent:
1) Khetha mocha oa FAM bakeng sa ho lemoha MPV;
2) Khetha mocha oa HEX / VIC bakeng sa ho lemoha liphatsa tsa lefutso tsa ka hare;
3.Tlhahlobo ea liphetho
Beha mola oa motheo ka holim'a ntlha e phahameng ka ho fetisisa ea mothinya oa fluorescent oa taolo e mpe.
4.Ho laola boleng
4.1 Taolo e fosahetseng: Ha ho boleng ba Ct bo fumanoeng ho FAM, HEX/VIC channel, kapa Ct＞40;
4.2 Taolo e ntle: Ho FAM, HEX/VIC kanale, Ct≤40;
4.3 Litlhoko tse ka holimo li lokela ho khotsofatsoa tekong e tšoanang, ho seng joalo liphetho tsa liteko li fosahetse 'me teko e lokela ho phetoa.
【Khaola boleng】
Mohlala o nkoa e le o motle ha: Tatellano ea sepheo Ct≤40, The internal control gene Ct≤40.
【Liphetho tlhaloso】
Hang ha taolo ea boleng e fetisitsoe, basebelisi ba lokela ho hlahloba hore na ho na le curve ea amplification bakeng sa sampole ka 'ngoe ho kanale ea HEX/VIC, haeba e teng le ka Ct≤40, e bonts'itse hore mofuta oa taolo ea kahare o matlafalitsoe ka katleho mme tlhahlobo ena e nepahetse. Basebelisi ba ka tsoela pele ho etsa tlhahlobo e latelang:
3. Bakeng sa disampole tse nang le amplification ea gene ea taolo ea ka hare e hlōlehile (HEX/VIC
channel, Ct＞40, kapa no amplification curve), low Viral load kapa ho ba teng ha PCR inhibitor e ka ba lebaka la ho hlōleha, tlhahlobo e lokela ho phetoa ho tsoa pokellong ea mohlala;
4.Bakeng sa disampole tse ntle le kokoana-hloko e hlahisitsoeng, liphello tsa taolo ea ka hare ha li ame;
Bakeng sa disampole tse fumanweng di se na HIV, taolo ya kahare e lokela ho lekolwa e le ntle, ho seng jwalo sephetho se fosahetse, mme tlhahlobo e lokela ho phetwa, ho qala ka mohato wa pokello ya mohlala.
Boitsebiso ba Pontšo
Setifikeiti sa Tlotlo
Boemo ba Khoebo
Rona, Hangzhou Testsea Biotechnology Co., Ltd ke k'hamphani e ntseng e hola ka potlako ea biotechnology e ikhethang ka ho etsa lipatlisiso, ho nts'etsapele, ho etsa le ho aba lisebelisoa tse tsoetseng pele tsa tlhahlobo ea in-vitro diagnostic(IVD) le lisebelisoa tsa bongaka.
Setsi sa rona ke GMP, ISO9001, le ISO13458 mme re na le tumello ea CE FDA. Hona joale re lebeletse ho sebelisana le lik'hamphani tse ling tsa mose ho maoatle bakeng sa nts'etsopele e kopanetsoeng.
Re hlahisa liteko tsa ho ba le bana, liteko tsa mafu a tšoaetsanoang, liteko tsa tšebeliso e mpe ea lithethefatsi, liteko tsa matšoao a pelo, liteko tsa matšoao a tumor, liteko tsa lijo le polokeho le liteko tsa mafu a liphoofolo, ho feta moo, mofuta oa rona oa TESTSEALABS o tsebahala haholo mebarakeng ea lapeng le mose ho maoatle. Litheko tsa boleng bo holimo le tse ntle li re thusa ho nka karolo ea 50% ea likarolo tsa lehae.
Mokhoa oa Sehlahisoa
1.Itokise
2.Sekoahelo
3.Lera la sefapano
4. Khaola hlobolisa
5.Kopano
6.Phakelang mekotla
7.Tiisa mekotla
8.Phakela lebokose
9.Encasement
Thibela Tlokotsi e Ncha: Itokise Joale ha Monkeypox e Nama
Ka August 14th, Mokhatlo oa Lefatše oa Bophelo (WHO) o phatlalalitse hore ho qhoma ha monkeypox ho na le "Boemo ba Tšohanyetso ba Bophelo bo Botle ba Matšoenyeho a Machaba." Lena ke lekhetlo la bobeli WHO e fana ka tlhokomeliso e phahameng ka ho fetisisa mabapi le seoa sa monkeypox ho tloha ka Phupu 2022.
Hajoale, seoa sa monkeypox se hasane ho tloha Afrika ho ea Europe le Asia, ka linyeoe tse netefalitsoeng li tlalehiloe Sweden le Pakistan.
Ho latela lintlha tsa morao-rao tse tsoang ho Africa CDC, selemong sena, linaha tse 12 tsa litho tsa African Union li tlalehile kakaretso ea linyeoe tse 18,737 tsa monkeypox, ho kenyeletsoa linyeoe tse 3,101 tse netefalitsoeng, linyeoe tse 15,636 tse belaelloang, le mafu a 541, ka sekhahla sa lefu la 2.89%.
01 Monkeypox ke eng?
Monkeypox (MPX) ke kokoana-hloko ea zoonotic e bakoang ke kokoana-hloko ea monkeypox. E ka fetisoa ho tloha liphoofolong ho ea ho batho, hammoho le pakeng tsa batho. Matšoao a tloaelehileng a kenyelletsa feberu, lekhopho le lymphadenopathy.
Kokoana-hloko ea monkeypox haholo-holo e kena 'meleng oa motho ka lera la mucous le letlalo le robehileng. Mehloli ea ts'oaetso e kenyelletsa linyeoe tsa monkeypox le litoeba tse tšoaelitsoeng, litšoene le litšoene tse ling tseo e seng batho. Ka mor'a tšoaetso, nako ea ho incubation ke matsatsi a 5 ho isa ho a 21, hangata matsatsi a 6 ho isa ho a 13.
Le hoja batho ka kakaretso ba hlaseloa ke kokoana-hloko ea monkeypox, ho na le tekanyo e itseng ea tšireletso khahlanong le monkeypox bakeng sa ba entetsoeng khahlanong le sekholopane, ka lebaka la ho tšoana ha liphatsa tsa lefutso le antigen pakeng tsa likokoana-hloko. Hona joale, monkeypox e hasana haholo har'a banna ba kopanelang liphate le banna ka ho kopanela liphate, ha kotsi ea tšoaetso bakeng sa batho ka kakaretso e ntse e le tlaase.
02 Seoa see sa Monkeypox se fapane joang?
Ho tloha qalong ea selemo, mofuta o ka sehloohong oa kokoana-hloko ea monkeypox, "Clade II," e bakile ho phatloha ho hoholo lefatšeng ka bophara. Se tšoenyang ke hore palo ea linyeoe tse bakoang ke "Clade I," e matla le ho feta, e nang le palo e phahameng ea batho ba shoang, e ntse e eketseha 'me e tiisitsoe ka ntle ho k'honthinente ea Afrika. Ho feta moo, ho tloha ka Loetse selemong se fetileng, mofuta o mocha, o kotsi le o fetisoang habonolo,"Clade Ib,” e se e qalile ho ata naheng ea Democratic Republic of the Congo.
Tšobotsi e hlokomelehang ea seoa sena ke hore basali le bana ba ka tlase ho lilemo tse 15 ke bona ba amehileng haholo.
Lintlha li bontša hore ho feta 70% ea linyeoe tse tlalehiloeng li ho bakuli ba ka tlase ho lilemo tse 15, 'me har'a linyeoe tse bolaeang, palo ena e nyolohela ho 85%. Haholo-holo,sekhahla sa ho shoa ha bana se phahame ka makhetlo a mane ho feta batho ba baholo.
03 Kotsi ea Phetiso ea Monkeypox ke Efe?
Ka lebaka la nako ea bahahlauli le litšebelisano tsa machaba khafetsa, kotsi ea ho fetisoa ha kokoana-hloko ea monkeypox e tšela meeli e ka eketseha. Leha ho le joalo, kokoana-hloko e hasana haholo-holo ka kamano e haufi ea nako e telele, e kang ho kopanela liphate, ho tšoara letlalo, le ho phefumoloha haufi-ufi kapa ho bua le ba bang, kahoo matla a eona a ho fetisetsa tšoaetso ho motho e mong ha a na matla.
04 Mokhoa oa ho Thibela Monkeypox?
Qoba ho kopanela liphate le batho bao boemo ba bona ba bophelo bo sa tsejoeng. Baeti ba lokela ho ela hloko seoa sa monkeypox linaheng le libaka tseo ba eang ho tsona mme ba qobe ho kopana le litoeba le litšoene.
Haeba boitšoaro bo kotsi haholo bo etsahala, itlhahlobe ka bophelo ba hau ka matsatsi a 21 'me u qobe ho kopana haufi le ba bang. Haeba matšoao a kang lekhopho, makhopho, kapa feberu a hlaha, batla thuso ea bongaka hang-hang ’me u tsebise ngaka ka boitšoaro bo loketseng.
Haeba setho sa lelapa kapa motsoalle a fumanoa a e-na le monkeypox, nka mehato ea ho itšireletsa, qoba ho kopana haufi-ufi le mokuli, ’me u se ke ua tšoara lintho tseo mokuli a li sebelisitseng, tse kang liaparo, likobo, lithaole le lintho tse ling tsa botho. Qoba ho arolelana likamore tsa ho hlapa, 'me hangata u hlatsoe matsoho le likamore tsa ho kenya moea.
Monkeypox Diagnostic Reagents
Li-reagents tsa ho hlahloba li-monkeypox li thusa ho netefatsa tšoaetso ka ho lemoha likokoana-hloko kapa li-antibodies, tse nolofalletsang ho itšehla thajana le mekhoa ea phekolo, le ho phetha karolo ea bohlokoa ho laola mafu a tšoaetsanoang. Hajoale, Anhui DeepBlue Medical Technology Co., Ltd. e hlahisitse lisebelisoa tse latelang tsa tlhahlobo ea monkeypox:
Monkeypox Antigen Test Kit: E sebelisa mokhoa oa khauta oa colloidal ho bokella mehlala e kang oropharyngeal swabs, nasopharyngeal swabs, kapa exudates ea letlalo bakeng sa ho fumanoa. E tiisa tšoaetso ka ho lemoha boteng ba li-antigens tsa kokoana-hloko.
Monkeypox Antibody Test Kit: E sebelisa mokhoa oa khauta ea colloidal, e nang le lisampole tse kenyelletsang mali a venous, plasma, kapa serum. E tiisa tšoaetso ka ho lemoha li-antibodies tse hlahisoang ke 'mele oa motho kapa oa phoofolo khahlanong le kokoana-hloko ea monkeypox.
Monkeypox Virus Nucleic Acid Test Kit: E sebelisa mokhoa oa PCR oa nako ea 'nete oa fluorescent,' me sampole e le lesion exudate. E tiisa tšoaetso ka ho lemoha genome ea kokoana-hloko kapa likaroloana tse itseng tsa lefutso.
Lihlahisoa tsa Tlhahlobo ea Monkeypox ea Testsealabs
Ho tloha ka 2015, li-reagents tsa tlhahlobo ea monkeypox tsa Testsealabs li netefalitsoe ho sebelisoa lisampole tsa 'nete tsa vaerase lilaboratoring tsa kantle ho naha mme li netefalitsoe ke CE ka lebaka la ts'ebetso ea tsona e tsitsitseng le e tšepahalang. Li-reagents tsena li shebile mefuta e fapaneng ea lisampole, tse fanang ka maikutlo a fapaneng le maemo a ikhethileng, li fana ka ts'ehetso e matla bakeng sa ho lemoha tšoaetso ea monkeypox le ho thusa hamolemo taolong e sebetsang ea seoa. Ho fumana lintlha tse ling mabapi le lisebelisoa tsa rona tsa tlhahlobo ea monkeypox, ka kopo hlahloba: https://www.tesselabs.com/monkeypox-virus-mpv-nucleic-acid-detection-kit-product/
Mokhoa oa teko
Ho sebelisa swab ho bokella boladu ho tswa ho pustule, ho e kopanya ka botlalo ka har'abuffer, ebe o sebelisa marotholi a seng makae kareteng ea tlhahlobo. Sephetho se ka fumanoa ka mehato e seng mekae feela e bonolo.
