SARS-CoV-2 Neutralizing Antibody Detection Kit (ELISA)
【ZVIRI KUSHANDISA】
SARS-CoV-2 Neutralizing Antibody Detection Kit iCompetitive Enzyme-Linked Immunosorbent Assay (ELISA) yakagadzirirwa kutariswa kwemhando uye semi-quantitative yekuona masoja ekudzivirira akazara kuSARS-CoV-2 muserum yevanhu uye plasma. Iyo SARS-CoV-2 Neutralizing Antibody Detection Kit inogona kushandiswa seyamuro mukuona vanhu vane inoshanduka immune immune kuSARS-CoV-2, zvichiratidza kutapukirwa kwazvino kana kusati kwaitika. Iyo SARS-CoV-2 Neutralizing Antibody Detection Kit haifanirwe kushandiswa kuongorora hutachiona hweSARS-CoV-2.
【NHUNGAMIDZO】
Utachiona hweCoronavirus hunowanzo konzeresa mhinduro dzekurwisa antibody. Huwandu hweseroconversion muvarwere veCCIDID-19 i50% uye zana muzana pazuva rechinomwe negumi nemana rekutanga chiratidzo, zvichiteerana. Kupa ruzivo, hutachiona hunoenderana hunoita kuti masoja ekudzivirira aite ropa muropa anocherechedzwa sechinangwa chekuona kushanda kwemasoja ekudzivirira chirwere uye kuwanda kwemasoja ekudzivirira chirwere anotaridza kuchengetedzwa kwepamusoro. Plaque Reduction Neutralization Test (PRNT) yanga ichicherechedzwa seye goridhe chiyero chekuona masoja ekudzivirira chirwere. Nekudaro, nekuda kwekuderera kwayo uye kudiwa kwepamusoro kwekushanda, PRNT haishande kune yakakura serodiagnosis uye kuongororwa kwejekiseni. Iyo SARS-CoV-2 Neutralizing Antibody Detection Kit yakavakirwa paCompetitive Enzyme-Linked Immunosorbent Assay (ELISA) nzira, iyo inogona kuona iyo isingachinjiki antibody musampuli yeropa pamwe nekunyanya kuwana mazinga eiyo erudzi urwu.
【Test Procedure】
1.Mumachubhu akasiyana, aliquot 120μL yehACE2-HRP Solution yakagadzirwa.
2.Wedzera 6 μL ye calibrator, sampuli dzisingazivikanwi, maitiro ehutano muhubhu imwe neimwe uye sanganisa zvakanaka.
3.Shandura 100μL yemusanganiswa wega wega wakagadzirirwa mudanho rechipiri mumatsime emicroplate anoenderana neyakafanorongwa bvunzo yekumisikidza.
3. Vhara ndiro nePlate Sealer uye isa pa 37 ° C kwemaminitsi makumi matanhatu.
4.Bvisa Plate Sealer uye ugeze ndiro neinenge 300 μL ye 1 × Wash Solution patsime kwechina.
5.Tapa ndiro pamucheka wepepa kuti ubvise mvura yakasara mumatsime mushure mekugeza matanho.
6.Wedzera 100 μL yeTMB Solution patsime rega rega uye isa ndiro murima pa20 - 25°C kwemaminitsi makumi maviri.
7.Wedzera 50 μL yeStop Solution kutsime rega rega kuti umise maitiro.
8. Verenga iyo absorbance mu microplate reader pa 450 nm mukati memaminitsi e10 (630nm sechinhu chinogadziriswa chinokurudzirwa kuti chiitwe zvakanyanya.
