Sars-cov-2 kusarerekera antibody yekuona kit (Elisa)
【Yakatarisirwa kushandiswa】
Sars-COV-2 Neutralizing Antibody Kit kit Imunoritive-linka Iyo Sars- COV-2 kusarerekera Anti Iyo inopfugama-cov-2 kusarerekera Anti
【Nhanganyaya】
Hutachiona hweCoronavirus hunowanzo kukonzera kusarerekera antibody mhinduro. Iyo seroconversion mitengo muCavid-19 varwere vari makumi mashanu muzana uye 100% pazuva rechinomwe 7 uye 14 post chiratidzo chekutanga, zvichiteerana. Kuti uburitse ruzivo, zvinoenderana hutachiona hwehutachiona hwehunhu muropa chinozivikanwa sechinangwa chekusarudza antibody yekushandisa uye nekumusoro kwepfungwa yeanorerekisa antilogy inoratidzira kugona kwekudzivirira kwakanyanya. Plaque kuderedza bvunzo yerarorization (prnt) yave kuzivikanwa segoridhe yegoridhe yekuona kusarerekera maantibodies ekutora. Nekudaro, nekuda kweiyo yakaderera yekuburitsa uye yakakwirira zvinodiwa kuti ishandiswe, prnt haina kushanda kune yakakura scaled serodiagnosis uye vaccine kuongororwa. Iyo inopfugama-cov-2 isina kurerutsa Anti
【Kuongorora maitiro】
1.Matsarisi anoparadzanisa, aliquot 120μl yeiyo yakagadzirirwa yeHACE2-HRP Solution.
2.Ad 6 μL ye calibrators, samples isingazivikanwe, mhando dzekudzora mune yega thubhu uye sanganisa zvakanaka.
3.RANSFer 100μL yeiyo imwe musanganiswa yakagadzirirwa mudanho 2 kuita inoenderana microplate matsime micres maererano nekufanorongerwa bvunzo.
3.CoT iyo ndiro ine mapani elate uye incubate pa37 ° C kwemakumi matanhatu emaminitsi.
4.ReMeventi iyo Plate Salor uye geza ndiro ine madhiri mazana matatu μL ye1
5.Pa ndiro papepa repepa kuti ubvise yakasara mvura mumatsime mushure mekugeza matanho.
6.Add 10 100 μL yeTMT Solution kune yega yega tsime uye incubate iyo ndiro murima pa20 - 25 ° C kwemaminetsi makumi maviri.
7.Dad 50 μl yemhinduro yekumisa kune yega yega yetsime kumira.
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