RSV Respiratory Syncytial Virus Ag Test

Tsanangudzo Pfupi:

Respiratory Syncytial Virus (RSV)utachiona hunonyanya kutapuriranwa hunonyanya kukanganisa nzira yekufema. Ichi ndicho chimwe chezvikonzero zvinowanzokonzera zvirwere zvekufema, kunyanya mucheche, vana vaduku, uye vanhu vakwegura. Zvirwere zveRSV zvinotangira kubva kunyoro, kutonhora-kufanana nezviratidzo kusvika kune zvakanyanya kufema zvirwere zvakadai sebronchiolitis uye pneumonia. Utachiona hunopararira kuburikidza nemadonhwe ekufema, kubata zvakananga, kana nzvimbo ine hutachiona. RSV inonyanya kuwanda munguva yechando uye kutanga kwemwedzi yechirimo, zvichiita kuti kuongororwa panguva uye kwakaringana kuve kwakakosha pakubata kwakanaka uye kutonga kwekubuda.

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Mhando dze RSV Miedzo:
- Rapid RSV Antigen Test:
  - Inoshandisa immunochromatographic lateral flow tekinoroji kukurumidza kuona maantigen eRSV mumasampuli ekufema (semuenzaniso, swabs dzemhino, huro).
  - Inopa mhinduro mukati15-20 maminitsi.
- RSV Molecular Test (PCR):
  - Inocherekedza RSV RNA ichishandisa zvakanyanya hunyanzvi hwemamorekuru senge reverse transcription-polymerase chain reaction (RT-PCR).
  - Inoda kushandiswa kwerabhoritari asi inopahigh senitivity uye chaiyo.
- RSV Viral Culture:
  - Inosanganisira kukura RSV munzvimbo inodzorwa lab.
  - Kashoma kushandiswa nekuda kwekureba kwenguva yekutendeuka.
Mhando dzemienzaniso:
- Nasopharyngeal swab
- Huro swab
- Nasal aspirate
- Bronchoalveolar lavage (yezviitiko zvakakomba)
Chinangwa Chevanhu:
- Vacheche nevana vadiki vanouya nezviratidzo zvakakomba zvekufema.
- Varwere vakwegura vane chirwere chekufema.
- Immunocompromised vanhu vane zviratidzo zvefuruwenza.
Mashandisiro Akawanda:
- Kusiyanisa RSV kubva kune zvimwe zvirwere zvekufema sefuruu, COVID-19, kana adenovirus.
- Kufambisa zvisarudzo zvekurapa panguva uye zvakakodzera.
- Kutariswa kwehutano hweveruzhinji panguva yekubuda kweRSV.

Musimboti:

Muedzo unoshandisaimmunochromatographic assay (lateral kuyerera)tekinoroji yekuona RSV maantigen.
Maantigen eRSV ari mumuenzaniso wekufema wemurwere anosungirira kune chaiwo masoja ekudzivirira chirwere akasanganiswa negoridhe kana zvimedu zvine ruvara pajira rekuyedza.
Mutsetse unooneka unoumba pamutsetse webvunzo (T) chinzvimbo kana maRSV antigens aripo.

Kuumbwa:

Composition	Mari	Tsanangudzo
IFU	1	/
Test cassette	25	/
Extraction diluent	500μL1 Tubhu 25	/
Drop tip	/	/
Swab	1	/

Test Procedure:


1. Geza maoko ako	2. Tarisa zviri mukati mekiti usati waedzwa, sanganisira package insert, test cassette, buffer, swab.	3.Isai chubhu yekubvisa munzvimbo yekushanda.	4.Bvisa aluminium foil seal kubva kumusoro kwechubhu yekubvisa ine buffer yekubvisa.

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5.Bvisa swab nekungwarira usina kubata muromo.Isa mboro yese yemboro 2 to 3cm mumhino yekurudyi.Cherechedza pakatyoka penasal.Unogona kuzvinzwa izvi neminwe yako pakupinza mhino kana kutarisa. zviri mu mimnor. Kwirai mukati memhino muchiita denderedzwa kashanu kweinenge masekondi gumi nemashanu,Zvino tora mhino imwe cheteyo woipinza mune imwe mhino.Sveta mukati memhino uchiita denderedzwa kashanu kweinenge masekondi gumi nemashanu. Ndapota ita bvunzo zvakananga nemuenzaniso uye usaite
chisiye chakamira.

6.Isa swab muchubhu yekubvisa.Dzungudza swab for about 10 seconds,Tenderedza swab pachubhu yekubvisa,udzvanyirira musoro wechubhu nemukati mechubhu uchipuruzira mativi echubhu kuti ibudise mvura yakawanda. sezvinobvira kubva kune swab.


7. Bvisa swab kubva pasuru pasina kubata padding.	8.Sanganisa zvakakwana nekupuruzira pasi pechubhu.Isa madonhwe matatu emuenzaniso wakatwasuka mutsime remuenzaniso wekaseti yebvunzo.Verenga chigumisiro mushure memaminitsi gumi nemashanu. Cherechedza: Verenga mhedzisiro mukati memaminitsi makumi maviri. Zvikasadaro, chikumbiro chebvunzo chinokurudzirwa.

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