Immunology inyaya yakaoma ine ruzivo rwakawanda rwehunyanzvi. Ichi chinyorwa chine chinangwa chekukuzivisa iwe kune zvigadzirwa zvedu zvinoshandisa mutauro mupfupi unonzwisisika.
Mundima yekukurumidza kuona, kushandiswa kwemba kunowanzo shandisa nzira yegoridhe colloidal.
Ndarama nanoparticles inosanganiswa nyore nyore kune masoja ekudzivirira chirwere, peptides, synthetic oligonucleotides, uye mamwe mapuroteni nekuda kwekubatana kwe sulfhydryl (-SH) mapoka epamusoro pegoridhe.3-5. Goridhe-biomolecule conjugates yakaverengerwa zvakanyanya mumashandisirwo ekuongorora, uko ruvara rwavo rutsvuku rwakajeka runoshandiswa kumba uye bvunzo-yekutarisira bvunzo senge bvunzo dzekumba.
Nekuti kushanda kuri nyore, mhedzisiro yacho iri nyore kunzwisisa, yakanakira, inokurumidza, yakarurama uye zvimwe zvikonzero. Colloidal goridhe nzira ndiyo nzira huru yekukurumidza yekuona pamusika.
Makwikwi uye masangweji assays ndiwo maviri makuru emhando mune colloidal goridhe nzira, Ivo vakwezva kufarira nekuda kwehushamwari mafomati emushandisi, nguva pfupi dzekuyedza, kupindira kudiki, kudhura kwakaderera, uye kuve nyore nekushandirwa nevasina hunyanzvi vashandi. Iyi nzira yakavakirwa pakusangana kwebiochemical yeantigen-antibody hybridization. Zvigadzirwa zvedu zvinoumbwa nezvikamu zvina: sampuli pad, iyo inzvimbo iyo sampu inodonhedzwa; conjugate pad, iyo yakanyorwa ma tag akasanganiswa nezvinhu zvebiorecognition; reaction membrane ine test line uye control line yeantigen-antibody interaction; uye padhi inonyudza, inochengeta tsvina.
1.Assay Principle
Masoja ekudzivirira chirwere maviri anosunga epitopes akasiyana aripo pamorekuru wehutachiona anoshandiswa. Imwe (yekuputira antibody) yakanyorwa necolloidal goridhe nanoparticles uye imwe (capture antibody) yakamisikidzwa panzvimbo yeNC membrane. Antibody yekuputira iri munzvimbo isina mvura mukati me conjugate pad. Kana yakajairwa mhinduro kana sampuli yakawedzerwa pane yemuenzaniso padhi yetest strip, binder inogona kunyungudutswa ipapo nekusangana neaqueous medium ine hutachiona. Ipapo antibody yakagadzira yakaoma nehutachiona muchikamu chemvura uye ichienda kumberi ichienderera kusvika yabatwa neantibody yakaiswa pameso eNC membrane, iyo yakagadzira chiratidzo muchikamu nezve hutachiona. Uyezve, imwe yekuwedzera antibody yakanangana neiyo yekuputira antibody inogona kushandiswa kugadzira chiratidzo chekudzora. Iyo inonwisa padhi inowanikwa kumusoro kukwezva necapillarity inoita kuti immune yakaoma idhonzwe kune yakagadziriswa antibody. Muvara unooneka wakaonekwa mune isingasviki 10 min, uye kusimba kunotaridza huwandu hwehutachiona. Mune mamwe mazwi, iyo yakawanda hutachiona hwaivepo mumuenzaniso, iyo yakanyanya kuoneka bhendi dzvuku rakaonekwa.
Rega nditsanangure muchidimbu kuti nzira mbiri idzi dzinoshanda sei:
1.Double anti sandwich nzira
Kaviri anti sandwich method method, inonyanya kushandiswa kuona mamolecular weight protein (anti). Maanti maviri anodiwa kunanga nzvimbo dzakasiyana dzeantigen.
2. Nzira yemakwikwi
Nzira yemakwikwi inoreva nzira yekuona antigen yakavharwa nemutsara wekuona uye antibody yegoridhe mark ye antigen inofanirwa kuongororwa.Zvigumisiro zvenzira iyi zvinoverengwa zvinopesana nemigumisiro ye sandwich nzira, neimwe mutsetse mune yakanaka uye mitsetse miviri mune inegetive.
Nguva yekutumira: Zvita-03-2019