Iwe unoziva sei kukurumidza bvunzo kit inoshanda?

Impunity inyaya yakaoma iyo ine yakawanda yehunyanzvi ruzivo. Chinyorwa ichi chinotarisira kukuzivisa iwe kuzvigadzirwa zvedu zvinoshandisa mutauro wenguva pfupi unonzwisisika.

Mundima yekukurumidza kuona, kushandiswa kwemba kunowanzoshandisa iyo collodal goridhe nzira.

Goridhe Nanoparticles inovharirwa kune Antibogades^3-5. Goridhe-biobolecule conjugates yakave yakabatanidzwa mune yekuongorora mairi, uko ruvara rwavo ruvara rwunoshandiswa mumusha nepfungwa-yemubhadharo wekutarisira mwekukutu

Nekuti kuvhiya kuri nyore, mhedzisiro iri nyore kunzwisisa, nyore, nekukurumidza, nekukurumidza uye zvimwe zvikonzero. Iyo Coldodal Gold nzira ndiyo yakakosha nekukurumidza kwekuona nzira pamusika.

Makwikwi uye Sandwich anoisa mwero maviri makuru eLollidal Gold nzira, ivo vanokwezva kufarira kwavo kushamwaridzana vashandisi, zvipfupi nguva, mutengo wakaderera, uye kuva nyore nekushandiswa nevashandi vasina-nyanzvi. Iyi nzira yakavakirwa pane biochemical yekudyidzana kweantigen-Antibody hybridization. Zvigadzirwa zvedu zvinoumbwa nezvikamu zvina: Iyo sampuli sampuli, iyo inzvimbo inodonhedzwa muenzaniso; conjugate pad, pane iyo yakanyorwa mabangi akasanganiswa ne biarecognition element; Reaction membrane ine test test mutsara uye kudzora mutsara wekutora antigen-anthidy kudyidzana; uye inonyudza pad, inochengetera marara.

1. 1.Sasay Pfungwa

Maviri antibodes achisunga akasiyana epitopes aripo pamushonga molecule anoshandiswa. Imwe (inobatanidza Anti Iyo yekubatanidza antibody iri mune yakasvibiswa mamiriro mukati mekonzo conjugate. Kana solath Solution kana sampuli yakawedzerwa pane sampu yekuenzanisira yebvunzo Ipapo antiari akagadzira yakaoma nehutachiona muchikamu chemvura uye akafamba achienderera mberi kusvikira yabatwa neAntio yakagadziriswa pane nzvimbo yeNC membrane, iyo yakaburitsa chiratidzo maererano neutachiona hwehutachiona. Uyezve, imwe yekuwedzera kurwisa yakanangana neyekubatanidza Anti Provide inogona kushandiswa kubereka chiratidzo chekutonga. Iyo yekubvisa pad inowanikwa kumusoro kuti inyore necullarity iyo inoita kuti ishandiswe isina kugadzirwa kuti ifambwe kune yakatarwa Anti Antti. Ruvara rwunooneka rwakaonekwa mune isingasviki 10 min, uye kukura kwacho kunosarudza huwandu hwehutachiona. Mune rimwe nerimwe izwi, hukuru hwakawanda hwakanga hwave huripo mune sampuli, iyo inoonekwa zvakanyanya kuti bhendi red red rakaonekwa.

Rega nditsanangure muchidimbu kuti nzira idzi mbiri dzinoshanda sei:

1.Dambudziko Anti Sandwich Nzira

Kaviri Anti Sandwich Nzira, inonyanya kushandiswa pakuonekwa kweiyo hombe molecular huremu hwehuremu (anti)

2. Makwikwi nzira

Nzira yemakwikwi inoreva nzira yekuona yekukwira kweanotsiwa neyekucherechedzwa mutsara weiyo antigen yeanorema. mutsara mune zvakanaka uye maviri mitsara mune yakaipa.