ICH-CPV-CDV IgG test kit

Iyo CANINE INFECTIOUS HEPATITIS/PARVO VIRUS/DISTEMPER VIRUS IgG ANTIBODY TEST KIT (ICH/CPV/CDV IgG test kit) yakagadzirirwa semi-quantitatively kuongorora imbwa IgG antibody mazinga eCanine Infectious Hepatitis Virus (ICH), Canine Parvo Virus (CPV) uye Canine Distemper Virus (CDV).

KIT ZVIRI MUKATI

KUGADZIRA UYE ZVINOTAURWA

Pane zvikamu zviviri zvakaputirwa mu cartridge yega yega: Kiyi, iyo inoiswa pamwe chete ne desiccant mukamuri yepasi yakavharwa neinodzivirira aluminium foil, uye kugadzira mhinduro, iyo inoiswa zvakasiyana mumakamuri epamusoro akavharwa neanodzivirira aluminium foil.

Imwe neimwe katuriji ine ese anodiwa reagents kune imwe sampuli yekuyedza. Nenguva pfupi, kiyi painopinzwa mukati uye nekumisikidzwa kwemaminetsi mashoma muchikamu chepamusoro 1, umo sampuli yeropa yakaiswa, iwo chaiwo maIgG ​​masoja ekudzivirira chirwere muropa rakanyungudutswa sampu, kana aripo, anosunga kuICH, CPV kana CDV recombinant antigens inomisikidzwa pane akasiyana discrete mavara pane akaiswa Kiyi. Ipapo kiyi inozoendeswa kune yasara macompartments epamusoro panguva dzakatarwa nhanho nhanho. Iwo akasungirirwa masoja ekudzivirira chirwere eIgG pamavara achanyorwa muchikamu chepamusoro che3, chine anti-canine IgG enzyme conjugate uye mibairo yekupedzisira inoratidzwa seruvara rwepepuru-bhuruu paKiyi ichagadzirwa kumusoro.

kamuri 6, ine substrate. Nokuda kwechigumisiro chinogutsa, matanho ekugeza anotangwa. Muchikamu chepamusoro chechipiri, iyo IgG isina muganhu uye zvimwe zvinhu mukati memuenzaniso weropa zvichabviswa. Muchikamu chepamusoro 4 ne5, iyo isina muganhu kana

yakawandisa anti-canine IgG enzyme conjugate ichabviswa zvakakwana. Pakupedzisira, muchikamu chepamusoro che7, chromosome yakawandisa yakagadzirwa kubva kune substrate uye yakasungwa enzyme conjugate muchikamu chepamusoro 6 ichabviswa. Kusimbisa chokwadi chekuita, puroteni yekudzora inounzwa panzvimbo yepamusoro paKiyi. Nzvimbo yepepuru-ruvara rwebhuruu inofanirwa kuoneka mushure mekupedza kuyedza kwakabudirira maitiro.

STORAGE

1. Chengetedza kiti pasi pefiriji yakajairika (2 ~ 8 ℃).

MUSAMUSA MAZWI KIT.

2. Kiti ine inactivated biological material. Iyo kit inofanira kubatwa

uye kuraswa maererano nezvinodiwa zveutsanana munharaunda.

TEST PROCEDURE

Kugadzirira usati waita bvunzo:

1. Uyai nekaturiji kune tembiricha yekamuri (20℃-30℃) uye uiise pabhenji rebasa kusvikira iyo thermal label pamadziro e cartridge yava ruvara rutsvuku.

2.Isa pepa rakachena retishu pabhenji rebasa rekuisa Kiyi.

3.Gadzirira 10μL dispenser uye 10μL standard pipette mazano.

4. Bvisa pasi pekudzivirira aluminium foil uye kanda Kiyi kunze kwechikamu chepasi che cartridge papepa rakachena retishu.

5. Mira wakatwasuka katuriji pabhenji rebasa uye simbisa kuti nhamba dzepamusoro dzekamuri dzinogona kuonekwa nenzira kwayo (matampu enhamba akarurama akatarisana newe). Tinya katuriji zvishoma kuti uve nechokwadi chekuti mhinduro dziri mumakamuri ekumusoro dzinodzokera pasi.

Kuita bvunzo:

1. Vhura mapepa ekudzivirira pamusoro pezvikamu zvepamusoro zvakanyatsonaka nechigunwe uye chigunwe kubva kuruboshwe kuenda kurudyi kusvikira CHETE uchifumura chikamu chepamusoro 1.

2.Kuwana sampuli yeropa yakaedzwa neine dispenser set uchishandisa chiyero 10μL pipette tip.

Pakuedza serum kana plasma shandisa 5μL.

Pakuongorora ropa rose shandisa 10μL.

EDTA kana heparin anticoagulant tubes inokurudzirwa kuplasma uye kuunganidza ropa rose.

3. Isa sampuli muchikamu chepamusoro 1. Zvadaro simudza uye udzikise dispenser plunger kakawanda kuti uwane kusanganisa (Chiedza chebhuruu chinogadzirisa mumuromo kana kusanganiswa kunoratidza dhipoziti yakabudirira).

4.Tonga Kiyi neMubati Wekiyi nemunwe wemberi uye nechigunwe nekungwarira woisa Kiyi mukamuri yepamusoro 1 (simbisa divi rinotonhora reKiyi rakatarisana newe, kana simbisa kuti semi-denderedzwa paHolder iri kurudyi kana wakatarisana. iwe). Zvadaro sanganisa uye umire Kiyi mukamuri yepamusoro 1 kwemaminitsi mashanu.

5. Vhura foiri yekudzivirira uchiramba uchienda kurudyi kusvikira CHETE uchifumura chikamu 2. Tora Kiyi neMubati woisa Kiyi mukamuri rakashama 2. Wosanganisa uye umise Kiyi muchikamu chepamusoro 2 kweminiti imwe.

6. Vhura foiri yekudzivirira uchiramba wakananga kurudyi kusvikira CHETE wafumura chikamu 3. Tora Kiyi neMubati woisa Kiyi mukamuri iri pachena 3. Wosanganisa uye umise Kiyi muchikamu 3 kwemaminitsi mashanu.

7. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 4. Tora Kiyi neMubati woisa Kiyi muchikamu chakavhurika 4. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 4 kweminiti imwe.

8. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 5. Tora Kiyi neMubati woisa Kiyi muchikamu chakavhurika 5. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 5 kweminiti 1.

9. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 6. Tora Kiyi neMubati woisa Kiyi mukamuri rakashama 6. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 6 kwemaminitsi mashanu.

10. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 7. Tora Kiyi neMubati woisa Kiyi mukamuri iri pachena 7. Wosanganisa uye umise Kiyi muchikamu chepamusoro 7 kweminiti imwe.

11. Bvisa Kiyi muchikamu chepamusoro chechinomwe wosiya kuti chiome pabepa rematishu kwemaminitsi angangoita mashanu usati waverenga zvabuda.

Notes:

Usabate iyo Frosting Side yeFront End yeKiyi, uko maantigen uye puroteni yekudzora isingafambireki (Muedzo uye Kudzora Dunhu).

Dzivisa kukwenya Dunhu reMuedzo uye Kudzora nekutsamira imwe Smooth Side yekumberi kweKiyi kumadziro emukati eimwe neimwe yepamusoro compartment uchisanganisa.

Kusanganisa, 10 nguva kusimudza uye kudzikisa Kiyi mune yega yega yepamusoro compartment inokurudzirwa.

CHETE Ratidza rinotevera repamusoro kamuri usati waendesa Kiyi.

Kana zvichidikanwa, batanidza iyo yakapihwa Pet Labels kune inopfuura imwe sampuli yekuyedzwa.

KUTURIRIRA ZVIBVUSWA ZVOKUYEDZERA

Tarisa nzvimbo dzakakonzerwa paKiyi neyakajairwa ColorScale

Hazvina basa:

HAPANA ruvara rwepepuru-bhuruu runoonekwa panzvimbo yekudzora

Negative(-)

HAPANA ruvara rwepepuru-bhuruu runoonekwa panzvimbo dzebvunzo

Zvakanaka (+)

Ruvara rwepepuru-ruvara runoonekwa panzvimbo dzebvunzo

Iwo matitter eiyo chaiyo IgG masoja ekudzivirira chirwere anogona kuenzanisirwa nematanho matatu