FLVFHVFCV IgG test kit

Iyo FELINE PANLEUKOPENIA/HERPES VIRUS/CALICI VIRUS IgG ANTIBODY TEST KIT (FPLV/FHV/FCV IgG test kit) yakagadzirirwa semi-quantitatively kuongorora katsi IgG antibody mazinga eFeline Panleunia (FPLV), Feline Herpes Virus uye Feline Fkopes Virus ( Hutachiona (FCV).

KIT ZVIRI MUKATI

KUGADZIRA UYE ZVINOTAURWA

Pane zvikamu zviviri zvakaputirwa mu cartridge yega yega: Kiyi, iyo inoiswa pamwe chete ne desiccant mukamuri yepasi yakavharwa neinodzivirira aluminium foil, uye kugadzira mhinduro, iyo inoiswa zvakasiyana mumakamuri epamusoro akavharwa neanodzivirira aluminium foil. Imwe neimwe katuriji ine ese anodiwa reagents kune imwe sampuli yekuyedza. Nenguva pfupi, kana Kiyi yaiswa mukati uye yakanyudzwa kwemaminetsi mashoma muchikamu chepamusoro 1, umo sampu yeropa yakaiswa, iwo chaiwo maIgG ​​masoja ekudzivirira eropa mune yakaderedzwa sampu yeropa, kana iripo, inosunga kuFPLV, FHV kana FCV recombinant antigens yakavharwa pane zvakasiyana

discrete spots paKiyi yakaiswa. Ipapo kiyi inozoendeswa kune yasara macompartments epamusoro panguva dzakatarwa nhanho nhanho. Iwo akasungirirwa masoja ekudzivirira chirwere eIgG pamavara achanyorwa muchikamu chepamusoro chechitatu, chine anti-feline IgG enzyme conjugate uye mibairo yekupedzisira inoratidzwa seruvara rwepepuru-bhuruu ichagadzirwa muchikamu chepamusoro chechitanhatu, icho chine substrate. Nokuda kwechigumisiro chinogutsa, matanho ekugeza anotangwa. Muchikamu chepamusoro chechipiri, iyo IgG isina muganhu uye zvimwe zvinhu mukati memuenzaniso weropa zvichabviswa. Muchikamu chepamusoro 4 uye 5, iyo isingaverengeki kana yakawandisa anti-feline IgG enzyme conjugate ichabviswa zvakakwana. Pakupedzisira, muchikamu chepamusoro che7, chromosome yakawandisa yakagadzirwa kubva kune substrate uye yakasungwa enzyme conjugate muchikamu chepamusoro 6 ichabviswa.

Kusimbisa chokwadi chekuita, puroteni yekudzora inounzwa panzvimbo yepamusoro paKiyi. Ruvara rwepepuru-ruvara rwebhuruu runofanira kuoneka mushure mekupedza chirongwa chakabudirira chekuyedza.

STORAGE

1. Chengetedza kiti pasi pefiriji yakajairika (2 ~ 8 ℃).

MUSAMUSA MAZWI KIT.

2. Kiti ine inactivated biological material. Iyo kit inofanira kubatwa

uye kuraswa maererano nezvinodiwa zveutsanana munharaunda.

TEST PROCEDURE

Kugadzirira usati waita bvunzo:

1. Uyai nekaturiji kune tembiricha yekamuri (20℃-30℃) uye uiise pabhenji rebasa kusvikira iyo thermal label pamadziro e cartridge yava ruvara rutsvuku.

2.Isa pepa rakachena retishu pabhenji rebasa rekuisa Kiyi.

3.Gadzirira 10μL dispenser uye 10μL standard pipette mazano.

4. Bvisa pasi pekudzivirira aluminium foil uye kanda Kiyi kunze kwechikamu chepasi che cartridge papepa rakachena retishu.

5. Mira wakatwasuka katuriji pabhenji rebasa uye simbisa kuti nhamba dzepamusoro dzekamuri dzinogona kuonekwa nenzira kwayo (matampu enhamba akarurama akatarisana newe). Tora katuriji zvishoma kuti uve nechokwadi chekuti

mhinduro mumakamuri ekumusoro anodzokera kuzasi.

Kuita bvunzo:

1. Vhura foil yekudzivirira iri pamakamuri ekumusoro zvakanyatsonaka nemunwe wekutanga uye chigunwe kubva kuruboshwe kuenda kurudyi kusvikira CHETE wafumura imba yepamusoro 1.

2.Kuwana sampuli yeropa yakaedzwa neine dispenser set uchishandisa chiyero 10μL pipette tip.

Pakuedza serum kana plasma shandisa 5μL.

Pakuongorora ropa rose shandisa 10μL.

EDTA kana heparin anticoagulant tubes inokurudzirwa kuplasma uye kuunganidza ropa rose.

3. Isa sampuli muchikamu chepamusoro 1. Zvadaro simudza uye udzikise dispenser plunger kakawanda kuti uwane kusanganisa (Chiedza chebhuruu chinogadzirisa mumuromo kana kusanganiswa kunoratidza dhipoziti yakabudirira).

4.Tonga Kiyi neMubati Wekiyi nemunwe wemberi uye nechigunwe nekungwarira woisa Kiyi mukamuri yepamusoro 1 (simbisa divi rinotonhora reKiyi rakatarisana newe, kana simbisa kuti semi-denderedzwa paHolder iri kurudyi kana wakatarisana. iwe). Zvadaro sanganisa uye umire Kiyi mukamuri yepamusoro 1 kwemaminitsi mashanu.

5. Vhura foil yekudzivirira uchiramba wakananga kurudyi kusvika CHETE wafumura chikamu 2. Tora Kiyi neMubati woisa Kiyi mukamuri iri pachena 2. Wosanganisa uye unomisa Kiyi mu

kumusoro kamuri 2 kweminiti 1.

6. Vhura foil yekudzivirira uchiramba wakananga kurudyi kusvikira CHETE wafumura chikamu 3. Tora Kiyi neMubati woisa Kiyi mukamuri iri pachena 3. Wosanganisa womisa Kiyi mu

kamuri 3 kwemaminitsi mashanu.

7. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 4. Tora Kiyi neMubati woisa Kiyi muchikamu chakavhurika 4. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 4 kweminiti imwe.

8. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 5. Tora Kiyi neMubati woisa Kiyi muchikamu chakavhurika 5. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 5 kweminiti 1.

9. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 6. Tora Kiyi neMubati woisa Kiyi mukamuri rakashama 6. Zvino sanganisa uye umise Kiyi muchikamu chepamusoro 6 kwemaminitsi mashanu.

10. Vhura foil yekudzivirira uchienderera mberi uchienda kurudyi kusvikira CHETE uchifumura chikamu 7. Tora Kiyi neMubati woisa Kiyi mukamuri iri pachena 7. Wosanganisa uye umise Kiyi muchikamu chepamusoro 7 kweminiti imwe.

11. Bvisa Kiyi muchikamu chepamusoro chechinomwe wosiya kuti chiome pabepa rematishu kwemaminitsi angangoita mashanu usati waverenga zvabuda.

 Notes:

Usabate iyo Frosting Side yeFront End yeKiyi, uko maantigen uye puroteni yekudzora isingafambireki (Muedzo uye Kudzora Dunhu).

Dzivisa kukwenya Dunhu reMuedzo uye Kudzora nekutsamira imwe Smooth Side yekumberi kweKiyi kumadziro emukati eimwe neimwe yepamusoro compartment uchisanganisa.

Kusanganisa, 10 nguva kusimudza uye kudzikisa Kiyi mune yega yega yepamusoro compartment inokurudzirwa.

CHETE Ratidza rinotevera repamusoro kamuri usati waendesa Kiyi.

Kana zvichidikanwa, batanidza iyo yakapihwa Pet Labels kune inopfuura imwe sampuli yekuyedzwa.

KUTURIRIRA ZVIBVUSWA ZVOKUYEDZERA

Tarisa nzvimbo dzakakonzerwa paKiyi neyakajairwa ColorScale

Hazvina basa:

HAPANA ruvara rwepepuru-bhuruu runoonekwa panzvimbo yekudzora

Negative(-)

HAPANA ruvara rwepepuru-bhuruu runoonekwa panzvimbo dzebvunzo

Zvakanaka (+)

Ruvara rwepepuru-ruvara runoonekwa panzvimbo dzebvunzo

Iwo matitter eiyo chaiyo IgG masoja ekudzivirira chirwere anogona kuenzanisirwa nematanho matatu