Ikizamini cyindwara ya Testsea Malariya Ag pf / pv Ikizamini cya Tri-umurongo

Indwara ya Testsea Indwara Malariya Ag pf / pv Tri-umurongo Ikizamini cyerekanwe

Ibisobanuro bigufi:

Intego:
Iki kizamini gitanga uburyo bwihuse kandi bwizewe bwo gusuzuma malariya yatewe naPlasmodium falciparumnaPlasmodium vivax. Itahura antigene yihariye ya malariya (nka HRP-2 kuri Pf na pLDH kuri Pv) igaragara mumaraso mugihe cyanduye cyane.

Ibintu by'ingenzi:

Igishushanyo cya Tri-Line:
- Iki kizamini kirashoboye kumenya byombiPlasmodium falciparum (Pf)naPlasmodium vivax (Pv)kwandura, hamwe n'imirongo itandukanye kuri buri bwoko n'umurongo wo kugenzura ubuziranenge.
Ibisubizo byihuse:
- Ibisubizo birahari gusaIminota 15-20, gukora bikwiriye gukoreshwa mu murima no gusuzuma-ingingo-yita ku bice bifite aho bigarukira muri laboratoire.
Ibyiyumvo Byinshi kandi Byihariye:
- Ikizamini cyateguwe kugirango umuntu yumve neza kandi yihariye mu kumenya antigene ya malariya, atanga ibisubizo nyabyo bifasha mu buvuzi bwa malariya.
Biroroshye gukoresha:
- Ikizamini gisaba imyitozo mike yo gukora kandi irakwiriye gukoreshwa mugace ka kure cyangwa ibikoresho bigarukira.

Ohereza imeri kuri twe Kuramo nka PDF

Ibicuruzwa birambuye

Ibicuruzwa

Ibicuruzwa birambuye:

Ubwoko bw'icyitegererezo:
- Amaraso yose (urutoki cyangwa venipuncture yamaraso).
Igihe cyo Kumenya:
- Iminota 15-20(ibisubizo bigomba gusobanurwa muminota 20; ibisubizo nyuma yiki gihe bitemewe).
Ibyiyumvo byihariye:
- Ibyiyumvo:Mubisanzwe> 90% mugutahura indwara zombi za Pf na Pv.
- Umwihariko:Mubisanzwe> 95% kuri Pf na Pv byombi.
Uburyo bwo kubika:
- Ubike hagati4 ° C na 30 ° C., kure y'izuba ritaziguye.
- Ntukonje.
- Ubuzima bwa Shelf busanzwe buvaAmezi 12 kugeza 24, ukurikije amabwiriza yabakozwe.
Ibisobanuro Ibisobanuro:
- Igisubizo cyiza:
  - Imirongo itatu iragaragara:
    1. C (Igenzura) umurongo(yerekana ikizamini gifite ishingiro).
    2. Umurongo wa Pf(niba hagaragaye antigen ya Plasmodium falciparum).
    3. Umurongo wa Pv(niba hagaragaye antigens ya Plasmodium vivax).
    - Kuba hari imirongo ya Pf na / cyangwa Pv byerekana kwandura amoko ya malariya.

Ihame:

Immunochromatographic Assay:
Cassette yikizamini irimo ubudahangarwaantibodies za monoclonalyihariye antigens ya Plasmodium (urugero,HRP-2kuri Pf napLDHkuri Pv).

Iyo amaraso ashyizwe mubizamini, nibamalariya antigenszirahari, zizahuza na antibodiyite ya zahabu muri sample, izagenda ikurikirana ikizamini cya capillary.
NibaPlasmodium falciparumantigen iramenyekana, umurongo wamabara uzakora kuriUmurongo wa Pf.
NibaPlasmodium vivaxantigen iramenyekana, umurongo wamabara uzakora kuriUmurongo wa Pv.
UwitekaUmurongo wo kugenzura (C)iremeza ko ikizamini gikora neza kandi cyerekana agaciro k'ikizamini.

Ibigize:

Ibigize	Umubare	Ibisobanuro
IFU	1	/
Ikizamini	25	Buri mufuka wifunze urimo igikoresho kimwe cyo gupima hamwe na desiccant
Gukuramo	500μL * 1 Tube * 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Inama	1	/
Swab	/	/

Uburyo bw'ikizamini:


1. Karaba intoki zawe	2. Reba ibikubiye mubikoresho mbere yo kwipimisha, shyiramo pake, cassette yikizamini, buffer, swab.	3. Shira umuyoboro wo gukuramo mu kazi.	4.Kuraho kashe ya aluminiyumu kuva hejuru yumuyoboro ukuramo urimo buffer.

1 (1)

5. Witonze ukureho swab udakoze ku isonga. Shyiramo isonga yose ya swab cm 2 kugeza kuri 3 mumazuru yiburyo. Andika aho kumena izuru. Urashobora kubyumva ukoresheje intoki zawe mugihe winjizamo izuru cyangwa ugenzura muri mimnor. Siga imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15, Noneho fata izuru rimwe hanyuma uzinjize mu zindi zuru. Shyira imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15. Nyamuneka kora ikizamini muburyo butaziguye kandi ntukore
kureka guhagarara.

6. Shira swab mu muyoboro wo gukuramo. Ongera uhindure swab mu masegonda agera ku 10, Hinduranya swab kurwanya umuyoboro ukuramo, ukande umutwe wa swab imbere imbere yigituba mugihe ukanda impande zumuyoboro kugirango urekure amazi menshi bishoboka uhereye kuri swab.


7. Kuramo swab muri paki udakora kuri padi.	8. Vanga neza ukanda hasi yigituba. Shyira ibitonyanga 3 byicyitegererezo uhagaritse kurugero rwicyitegererezo cya cassette. Soma ibisubizo nyuma yiminota 15. Icyitonderwa: Soma ibisubizo muminota 20. Ubundi, ni ugusaba ikizamini birasabwa.

Ibisubizo Ibisobanuro: