Ikizamini cya Indwara ya Testsea VIH 1/2 Ikizamini cyihuta

Ibisobanuro bigufi:

Virusi ya immunodeficiency ya muntu (VIH)ni virusi yibasira sisitemu yumubiri, yibasira cyaneCD4 + T.(bizwi kandi nka T-umufasha selile), nibyingenzi mukurinda ubudahangarwa. Iyo itavuwe, virusi itera SIDA irashobora guteraIndwara ya Immunodeficiency Syndrome (SIDA), imiterere aho sisitemu yumubiri yangiritse cyane kandi idashobora kurwanya indwara nindwara.

VIH yandura cyane cyanemaraso, amasohoro, amazi yo mu gitsina, urukiramende, naamashereka. Inzira zikwirakwizwa cyane zirimo guhuza ibitsina bidakingiye, gusangira inshinge zanduye, no kwanduza nyina ku mwana mugihe cyo kubyara cyangwa konsa.

Hariho ubwoko bubiri bw'ingenzi bwa virusi itera SIDA:

VIH-1:Ubwoko bwa virusi itera SIDA ku isi hose.
VIH-2:Ntibisanzwe, cyane cyane biboneka muri Afrika yuburengerazuba, kandi mubisanzwe bifitanye isano no kugenda buhoro buhoro sida.

Kumenya hakiri kare no kuvura hamweimiti igabanya ubukana bwa virusi (ART)irashobora gufasha abantu banduye virusi itera sida kubaho igihe kirekire, kizima kandi bikagabanya cyane ibyago byo kwanduza abandi.

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Ibicuruzwa birambuye

Ibicuruzwa

Ibicuruzwa birambuye:

Ibyiyumvo Byinshi kandi Byihariye
Ikizamini cyateguwe kugirango hamenyekane neza antibodiyite zombi VIH-1 na VIH-2, zitange ibisubizo byizewe hamwe na reaction nkeya.
Ibisubizo Byihuse
Ibisubizo biraboneka muminota 15-20, bigafasha gufata ibyemezo byubuvuzi no kugabanya igihe cyo gutegereza abarwayi.
Kuborohereza gukoreshwa
Igishushanyo cyoroshye kandi cyorohereza abakoresha, gisaba nta bikoresho byihariye cyangwa amahugurwa. Birakwiye gukoreshwa mumyanya yubuvuzi hamwe n’ahantu hitaruye.
Ubwoko bw'icyitegererezo
Ikizamini gihuye namaraso yose, serumu, cyangwa plasma, bitanga ihinduka mugukusanya icyitegererezo no kongera urwego rwibisabwa.
Igendanwa hamwe na Porogaramu
Byoroheje kandi biremereye, bituma ibikoresho byo kwipimisha biba byiza muburyo bwo kwitaho, amavuriro yubuzima bugendanwa, hamwe na gahunda yo gusuzuma.

Ihame:

Icyegeranyo cy'icyitegererezo
Umubare muto wa serumu, plasma, cyangwa amaraso yose ushyirwa kumurongo wicyitegererezo cyibikoresho bipimisha, hanyuma hakongerwaho igisubizo cya buffer kugirango utangire inzira yikizamini.
Imikoreshereze ya Antigen-Antibody
Ikizamini kirimo antijene za recombinant kuri virusi ya VIH-1 na VIH-2, zanduzwa mu karere k’ibizamini bya membrane. Niba antibodiyite zanduye virusi itera SIDA (IgG, IgM, cyangwa zombi) zihari murugero, zizahuza na antigene kuri membrane, zikora antigen-antibody.
Kwimuka kwa Chromatographic
Antigen-antibody igizwe na membrane ikoresheje capillary action. Niba antibodiyite za sida zihari, urwego ruzahuza umurongo wikizamini (T umurongo), rutange umurongo wamabara agaragara. Reagents zisigaye zimukira kumurongo ugenzura (C umurongo) kugirango zemeze neza ikizamini.
Ibisobanuro
- Imirongo ibiri (T umurongo + C umurongo):Igisubizo cyiza, cyerekana ko hariho antibodiyite ya VIH-1 na / cyangwa VIH-2.
- Umurongo umwe (C umurongo gusa):Ibisubizo bibi, byerekana ko nta antibodiyite zanduye zanduye.
- Nta murongo cyangwa T umurongo gusa:Ibisubizo bitemewe, bisaba ikizamini gisubirwamo.

Ibigize:

Ibigize	Umubare	Ibisobanuro
IFU	1	/
Ikizamini	1	Buri mufuka wifunze urimo igikoresho kimwe cyo gupima hamwe na desiccant
Gukuramo	500μL * 1 Tube * 25	Tris-Cl buffer, NaCl, NP 40, ProClin 300
Inama	1	/
Swab	1	/

Uburyo bw'ikizamini:


1. Karaba intoki zawe	2. Reba ibikubiye mubikoresho mbere yo kwipimisha, shyiramo pake, cassette yikizamini, buffer, swab.	3. Shira umuyoboro wo gukuramo mu kazi.	4.Kuraho kashe ya aluminiyumu kuva hejuru yumuyoboro ukuramo urimo buffer.

1 (1)

5. Witonze ukureho swab udakoze ku isonga. Shyiramo isonga yose ya swab cm 2 kugeza kuri 3 mumazuru yiburyo. Andika aho kumena izuru. Urashobora kubyumva ukoresheje intoki zawe mugihe winjizamo izuru cyangwa ugenzura muri mimnor. Siga imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15, Noneho fata izuru rimwe hanyuma uzinjize mu zindi zuru. Shyira imbere yizuru mumuzenguruko inshuro 5 byibuze amasegonda 15. Nyamuneka kora ikizamini muburyo butaziguye kandi ntukore
kureka guhagarara.

6. Shira swab mu muyoboro wo gukuramo. Ongera uhindure swab mu masegonda agera ku 10, Hinduranya swab kurwanya umuyoboro ukuramo, ukande umutwe wa swab imbere imbere yigituba mugihe ukanda impande zumuyoboro kugirango urekure amazi menshi bishoboka uhereye kuri swab.


7. Kuramo swab muri paki udakora kuri padi.	8. Vanga neza ukanda hasi yigituba. Shyira ibitonyanga 3 byicyitegererezo uhagaritse kurugero rwicyitegererezo cya cassette. Soma ibisubizo nyuma yiminota 15. Icyitonderwa: Soma ibisubizo muminota 20. Ubundi, ni ugusaba ikizamini birasabwa.

Ibisubizo Ibisobanuro: