SARS-CoV-2 Kutabogama antibody Ikizamini Cassette
Video
Kugirango usuzume neza indwara ya Coronavirus 2019 (2019-nCOV cyangwa COVID -19) itesha agaciro antibody muri serumu yumuntu / plasma / maraso yose.
Kubanyamwuga Muri Vitro Gusuzuma Koresha Byonyine
GUKORESHA】
SARS-CoV-2 Kutabogama antibody Ikizamini Cassette ni chromatografi yihuta
immunoassay kugirango hamenyekane ubuziranenge bwa antibody ya Coronavirus Disease 2019 mumaraso yumuntu yose, serumu, cyangwa plasma nkubufasha murwego rwo gusuzuma Isuzuma ryabantu barwanya udushya coronavirus itesha antibody titer.
inyamaswa z’inyamabere. Ubwoko γ butera cyane kwandura inyoni.CoV yandura cyane cyane binyuze muburyo butaziguye n'amasohoro cyangwa binyuze muri aerosole n'ibitonyanga. Hariho kandi ibimenyetso byerekana ko ishobora kwanduzwa binyuze munzira ya fecal-umunwa.
Indwara ikaze yubuhumekero bukabije coronavirus 2 (SARS-CoV-2, cyangwa 2019-nCoV) ni virusi ya RNA itwikiriwe neza. Nintandaro yindwara ya coronavirus 2019 (COVID- 19), yanduza abantu.
SARS-CoV-2 ifite poroteyine nyinshi zubaka zirimo spike (S), ibahasha (E), membrane (M) na nucleocapsid (N). Intungamubiri za spike (S) zirimo reseptor ihuza indangarubuga (RBD), ishinzwe kumenya reseptor ya selile, angiotensin ihindura enzyme-2 (ACE2). Byagaragaye ko RBD ya poroteyine ya SARS-CoV-2 S ikorana cyane na reseptor ya ACE2 yumuntu iganisha kuri endocytose mu ngirabuzimafatizo zakira ibihaha byimbitse no kwigana virusi.
Kwandura SARS-CoV-2 bitangiza ubudahangarwa bw'umubiri, burimo no gukora antibodies mumaraso. Antibodies zasohotse zitanga uburinzi bwokwirinda kwandura virusi, kubera ko ziguma mumikorere yizunguruka mumezi kugeza kumyaka nyuma yo kwandura kandi zizahuza vuba na bwangu na virusi kugirango ihagarike kwinjirira kwa selile no kuyigana. Izi antibodies zitwa kutabuza antibodies.
GUKORANYA BIDASANZWE NO GUTEGURA】
1.SARS-CoV-2 Kutabogama antibody Ikizamini Cassette igenewe gukoreshwa hamwe namaraso yumuntu, serumu cyangwa plasma gusa.
2.Gusobanura neza, kutagira hemolyzed ingero zirasabwa gukoreshwa niki kizamini. Serumu cyangwa plasma bigomba gutandukana vuba bishoboka kugirango wirinde hemolysis.
3.Kora ikizamini ako kanya nyuma yo gukusanya ingero. Ntugasige ingero mubushyuhe bwicyumba igihe kirekire. Ingero za serumu na plasma zirashobora kubikwa kuri 2-8 ° C mugihe cyiminsi 3. Kubikwa igihe kirekire, urugero rwa serumu cyangwa plasma bigomba kubikwa munsi ya 20 ° C. Amaraso yose yakusanyirijwe hamwe na venipuncture agomba kubikwa kuri 2-8 ° C niba ikizamini kigomba gukorwa mugihe cyiminsi 2 nyuma yo gukusanya.Ntugahagarike amaraso yose ingero. Amaraso yose yakusanyijwe nintoki agomba guhita apimwa.
4.Ibikoresho birimo anticoagulants nka EDTA, citrate, cyangwa heparin bigomba gukoreshwa mububiko bwamaraso bwose. Kuzana ingero mubushyuhe bwicyumba mbere yo kwipimisha.
5.Ikigereranyo cyakonjeshejwe kigomba gukonjeshwa rwose no kuvangwa neza mbere yo kwipimisha. Irinde gukonjesha inshuro nyinshi
no gukonjesha ingero.
6.Niba ingero zigomba koherezwa, uzipakire hubahirijwe amabwiriza yose akoreshwa mu gutwara abantu
ya etiologiya.
7.Icteric, lipemic, hemolyzed, ubushyuhe buvuwe na sera yanduye birashobora gutera ibisubizo bibi.
8.Iyo gukusanya amaraso y'urutoki hamwe na lancet na padi ya alcool, Nyamuneka ujugunye igitonyanga cya mbere cya
maraso yose.
1. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura. Kuraho igikoresho cyipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka.
2. Shira igikoresho cyo kwipimisha hejuru kandi isukuye.
Kuri Serumu cyangwa Plasma Ibigereranyo: Ukoresheje Micropipette, hanyuma wohereze 5ul serumu / plasma kurugero rwiza rwibikoresho byipimisha, hanyuma ongeramo 2 igitonyanga cya buffer, hanyuma utangire igihe.
Kumaraso Yuzuye (Venipuncture / Urutoki) Ibigereranyo: Kata urutoki rwawe hanyuma ukande urutoki witonze, koresha umuyoboro wa pulasitike watanzwe kugirango unywe 10ul yamaraso yose kumurongo wa 10ul kumurongo wa pulasitike ikoreshwa, hanyuma uyohereze mumwobo wikigereranyo cyibikoresho bipimisha (niba ubwinshi bwamaraso arenze ikimenyetso, Nyamuneka urekure amaraso arenze muri pipette), hanyuma ongeraho ibitonyanga 2 bya buffer, hanyuma utangire igihe. Icyitonderwa: Ibigereranyo birashobora kandi gukoreshwa ukoresheje micropipette.
3. Tegereza umurongo (amabara) ugaragara. Soma ibisubizo muminota 15. Ntugasobanure ibisubizo nyuma yiminota 20.
