SARS-CoV-2 Kutabogama Ibikoresho bya Antibody （ELISA)
【UKORESHEJWE】
SARS-CoV-2 Kutabogama Antibody Detection Kit nigikoresho cyo guhiganwa Enzyme ihujwe na Immunosorbent Assay (ELISA) igamije kumenya ubuziranenge hamwe na kimwe cya kabiri cyo kumenya antibodiyide zose zangiza SARS-CoV-2 muri serumu na plasma. SARS- CoV-2 Kutabogama kwa Antibody Detection Kit birashobora gukoreshwa nkubufasha mugutahura abantu bafite ubudahangarwa bw'umubiri kuri SARS- CoV-2, byerekana kwandura vuba cyangwa mbere. SARS-CoV-2 Kutabogama Antibody Detection Kit ntigomba gukoreshwa mugupima indwara ikaze ya SARS-CoV-2.
【IRIBURIRO】
Indwara ya Coronavirus mubisanzwe itera kutabogama kwa antibody. Igipimo cya serokonversion ku barwayi ba COVID-19 ni 50% na 100% kumunsi wa 7 na 14 nyuma yo gutangira ibimenyetso. Kugirango ugaragaze ubumenyi, virusi ihuye na antibody mu maraso izwi nkintego yo kumenya imikorere ya antibody hamwe nubunini bwinshi bwa antibody itabogamye byerekana ingaruka nziza zo kurinda. Ikizamini cyo kugabanya kutabogama kwa Plaque (PRNT) cyamenyekanye nkigipimo cya zahabu cyo kumenya antibodiyite zidafite aho zibogamiye. Ariko, kubera ibicuruzwa byinjira bike kandi bisabwa cyane kugirango bikorwe, PRNT ntabwo ari ingirakamaro kuri serodiagnose nini no gusuzuma inkingo. SARS-CoV-2 Kutabogama Antibody Detection Kit ishingiye ku buryo bwo Kurwanya Enzyme-Ihuza Immunosorbent Assay (ELISA), ishobora kumenya antibody itabogamye mu maraso ndetse ikanagera ku buryo bwihariye bwo kwibanda kuri ubu bwoko bwa antibody.
【Uburyo bwo Kwipimisha】
1.Mu tubari dutandukanye, aliquot 120μL yumuti wateguwe HACE2-HRP.
2. Ongeraho 6 μL ya kalibatori, ingero zitazwi, kugenzura ubuziranenge muri buri tube hanyuma uvange neza.
3.Kwimura 100μL ya buri mvange yateguwe muntambwe ya 2 mumariba ya microplate ihuye ukurikije ibizamini byateganijwe mbere.
3.Gupfundikanya isahani hamwe na Plate Sealer hanyuma ushire kuri 37 ° C muminota 60.
4.Kuraho icyapa cya plaque hanyuma ukarabe isahani hamwe hafi 300 μL ya 1 × Gukaraba igisubizo kuri buri riba inshuro enye.
5.Kanda isahani kumpapuro kugirango ukureho amazi asigaye mumariba nyuma yo koza intambwe.
6. Ongeraho 100 μL ya TMB Igisubizo kuri buri riba hanyuma ushireho isahani mwijimye kuri 20 - 25 ° C muminota 20.
7. Ongeraho 50 μL yo guhagarika igisubizo kuri buri riba kugirango uhagarike reaction.
8.Soma ibyinjira mubasomyi ba microplate kuri 450 nm muminota 10 (630nm nkuko ibikoresho bisabwa kugirango bikore neza.
