Intambwe imwe SARS-CoV2 (COVID-19) Ikizamini cya IgG / IgM
Gukoresha
Intambwe imwe SARS-CoV2 (COVID-19) Ikizamini cya IgG / IgM ni immunoassay yihuse ya chromatografique kugirango hamenyekane neza antibodies (IgG na IgM) kugeza virusi ya COVID-19 muri Blood Blood / Serum / Plasma kugirango ifashe mugupima COVID -19 kwandura virusi.
Incamake
Virusi ya Corona ifunitse virusi ya RNA ikwirakwizwa cyane mubantu, izindi nyamaswa z’inyamabere, n’inyoni kandi zitera indwara z'ubuhumekero, iz'inda, iz'umwijima na neurologiya.Ubwoko burindwi bwa virusi ya corona buzwiho gutera indwara zabantu.Virusi enye-229E.OC43.NL63 na HKu1- biriganje kandi mubisanzwe bitera ibimenyetso bikonje bikabije kubantu badafite ubudahangarwa.4 Ubundi buryo butatu bukomeye cyane syndrome de syndrome de corporovirus (SARS-Cov), syndrome de syndrome de coronavirus yo mu burasirazuba bwo hagati (MERS-Cov) na 2019 Novel Coronavirus (COVID- 19) - ni zoonotic inkomoko kandi yagiye ihura nindwara zica rimwe na rimwe.Antibodies za IgG na lgM kugeza muri 2019 Novel Coronavirus irashobora kumenyekana nyuma y'ibyumweru 2-3 nyuma yo guhura.lgG ikomeza kuba nziza, ariko urwego rwa antibody rugabanuka amasaha y'ikirenga.
Ihame
Intambwe imwe SARS-CoV2 (COVID-19) IgG / IgM (Amaraso Yose / Serumu / Plasma) ni imigezi ikurikira immunochromatographic assay.Ikizamini gikoresha antibody irwanya abantu (umurongo wikizamini IgM), irwanya abantu lgG (umurongo wikizamini lgG nihene irwanya urukwavu igG (umurongo ugenzura C) yimuwe ku gipande cya nitrocellulose. COVID-19 antigens ihujwe na zahabu ya colloid (COVID-19 conjugatesand urukwavu lgG-zahabu conjugate. Iyo urugero rwakurikiwe na buffer assay rwongewe kurugero rwiza, antibodies za IgM & / cyangwa lgG niba zihari, zizahuza na COVID-19 zikora antigen antibodies complex. ibisubizo byikizamini cyibisubizo.
Ikizamini kirimo igenzura ryimbere (C band) rigomba kwerekana ibara ryamabara ya burgundy yihene irwanya urukwavu IgG / urukwavu lgG-zahabu conjugate utitaye kumabara yibara kuri buri tsinda ryipimishije.Bitabaye ibyo, ibisubizo byikizamini ntabwo byemewe kandi icyitegererezo kigomba gusubirwamo hamwe nibindi bikoresho.
Kubika no Guhagarara
- Ubike nkuko bipakiye mumufuka ufunze mubushyuhe bwicyumba cyangwa firigo (4-30 ℃ cyangwa 40-86 ℉).Igikoresho cyo kwipimisha gihamye binyuze mumatariki yo kurangiriraho yacapishijwe kumufuka ufunze.
- Ikizamini kigomba kuguma mu gikapu gifunze kugeza gikoreshejwe.
Ibikoresho byihariye
Ibikoresho byatanzwe:
| .Ibikoresho bipimisha | .Ikoreshwa ryikigereranyo |
| .Buffer | .Ongeramo paki |
Ibikoresho bisabwa ariko ntibitangwa:
| .Centrifuge | .Igihe |
| .Inzoga | .Ibikoresho byo gukusanya |
Kwirinda
☆ Kubanyamwuga muri vitro yo gusuzuma gusa.Ntukoreshe nyuma yitariki yo kurangiriraho.
Not Ntukarye, unywe cyangwa unywa itabi ahantu hakorerwa ingero n'ibikoresho.
Koresha ingero zose nkaho zirimo ibintu byanduza.
. Kurikiza ingamba zashyizweho zo kwirinda ingaruka ziterwa na mikorobe mu nzira zose kandi ukurikize inzira zisanzwe zo kujugunya neza ingero.
Wambare imyenda ikingira nk'amakoti ya laboratoire, uturindantoki twajugunywe hamwe no kurinda amaso mugihe ingero zapimwe.
Kurikiza amabwiriza asanzwe ya bio-umutekano yo gutunganya no kujugunya ibintu byanduye.
Ubushuhe n'ubushuhe birashobora kugira ingaruka mbi kubisubizo.
Icyegeranyo cyo gukusanya no kwitegura
1. Ikizamini cya SARS-CoV2 (COVID-19) IgG / IgM Ikizamini gishobora gukorwa ku maraso Yuzuye / Serumu / Plasma.
2. Gukusanya amaraso yose, serumu cyangwa plasma ikurikiza uburyo busanzwe bwa laboratoire.
3. Kwipimisha bigomba gukorwa ako kanya nyuma yo gukusanya ingero.Ntugasige ingero zubushyuhe bwicyumba igihe kirekire.Kubikwa igihe kirekire, ingero zigomba kubikwa munsi -20 ℃.Amaraso yose agomba kubikwa kuri 2-8 ℃ niba ikizamini kigomba gukorwa muminsi 2 yo gukusanya.Ntugahagarike urugero rwamaraso yose.
4. Zana ingero z'ubushyuhe bw'icyumba mbere yo kwipimisha.Ingero zikonje zigomba gukonjeshwa rwose no kuvangwa neza mbere yo kwipimisha.Ingero ntizigomba gukonjeshwa no gukonjeshwa inshuro nyinshi.
Uburyo bwo Kwipimisha
1. Emerera ikizamini, ingero, buffer na / cyangwa kugenzura kugera ku bushyuhe bwicyumba 15-30 ℃ (59-86 ℉) mbere yo kwipimisha.
2. Zana umufuka mubushyuhe bwicyumba mbere yo gufungura.Kuraho igikoresho cyo kwipimisha mumufuka ufunze hanyuma ukoreshe vuba bishoboka.
3. Shira igikoresho cyo kwipimisha hejuru kandi isukuye.
4. Fata igitonyanga gihagaritse hanyuma wohereze igitonyanga 1 cyikigereranyo (hafi 10μl) kurugero rwiza (S) rwibikoresho byipimisha, hanyuma wongereho ibitonyanga 2 bya buffer (hafi 70μl) hanyuma utangire igihe.Reba ingero zikurikira.
5. Tegereza umurongo (amabara) ugaragara.Soma ibisubizo muminota 15.Ntugasobanure ibisubizo nyuma yiminota 20.
Inyandiko:
Gukoresha urugero ruhagije rwicyitegererezo ni ngombwa kubisubizo byemewe.Niba kwimuka (guhanagura kwa membrane) bitagaragaye mumadirishya yikizamini nyuma yiminota umwe, ongeramo ikindi gitonyanga cya buffer kurugero rwiza.
Gusobanura ibisubizo
Ibyiza:Kugenzura umurongo kandi byibuze umurongo umwe wikizamini ugaragara kuri membrane.Kugaragara kwa T2 umurongo wikizamini byerekana ko hariho antibodiyite za COVID-19 zihariye.Kugaragara kumurongo wa T1 yerekana ko hariho antibodiyite za COVID-19 zihariye.Niba kandi umurongo wa T1 na T2 ugaragara, byerekana ko kuba COVID-19 yihariye ya antibodiyite IgG na IgM.Hasi ya antibody yibanze ni, intege nke ibisubizo ni.
Ibibi:Umurongo umwe wamabara ugaragara mukarere kayobora (C) .Nta murongo ugaragara wamabara ugaragara mukarere kizamini.
Ntibyemewe:Igenzura ryananiwe kugaragara.Ingano yikigereranyo idahagije cyangwa tekinike yuburyo bukwiye nimpamvu zishoboka zo kugenzura umurongo kunanirwa.Ongera usubiremo inzira hanyuma usubiremo ikizamini ukoresheje igikoresho gishya.Niba ikibazo gikomeje, hagarika ako kanya ukoresheje ibikoresho byipimisha ako kanya hanyuma ubaze abakwirakwiza hafi.
Imipaka
1.Ikizamini cya SARS-CoV2 (COVID-19) IgG / IgM ni mugukoresha vitro yo gusuzuma gusa.Ikizamini kigomba gukoreshwa mugutahura antibodiyite za COVID-19 mumaraso Yuzuye / Serumu / Plasma gusa.Yaba agaciro k'umubare cyangwa igipimo cyo kwiyongera muri 2. Antibodiyite COVID-19 ntishobora kugenwa n'iki kizamini cyujuje ubuziranenge.
3. Kimwe nibizamini byose byo gusuzuma, ibisubizo byose bigomba gusobanurwa hamwe nandi makuru yubuvuzi aboneka kwa muganga.
4. Niba ibisubizo byikizamini ari bibi kandi ibimenyetso byubuvuzi bikomeje, birasabwa kwipimisha ukoresheje ubundi buryo bwo kwa muganga.Igisubizo kibi ntikibuza umwanya uwariwo wose kwandura virusi ya COVID-19.
Amakuru yimurikabikorwa
Icyemezo cy'icyubahiro
Umwirondoro w'isosiyete
Twebwe, Hangzhou Testsea Biotechnology Co., Ltd nisosiyete ikora ibijyanye n’ibinyabuzima byihuta cyane mu bushakashatsi, guteza imbere, gukora no gukwirakwiza ibikoresho byipimishije muri vitro bisuzumwa (IVD) n'ibikoresho by'ubuvuzi.
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