SARS-CoV-2 Igihe nyacyo RT-PCR Kumenya

Iki gikoresho kigenewe muri vitro yujuje ubuziranenge bwa genF ya ORF1ab na N kuva muri 2019-nCoV muri pharyngeal swab cyangwa bronchoalveolar lavage ingero yakusanyirijwe mu ndwara ya Coronavirus 2019 (COVID-19) ikekwaho ibibazo, abakekwaho kuba ari imanza, cyangwa abandi bantu bakeneye 2019 - nCoV kwisuzumisha kwandura cyangwa gusuzuma itandukaniro.

Igikoresho cyagenewe RNA gutahura 2019-nCoV mubigereranyo ukoresheje multiplex nyayo igihe cya tekinoroji ya RTPCR hamwe nibice byabitswe bya genF ya ORF1ab na N nkibibanza bigenewe primers na probe. Icyarimwe, iki gikoresho kirimo sisitemu yo kugenzura igenzura (gene igenzurwa na Cy5) kugirango ikurikirane inzira yo gukusanya ingero, gukuramo aside nucleic na PCR no kugabanya ibisubizo bibi.

Ibintu by'ingenzi:

1.

2. Intambwe imwe RT-PCR reagent (ifu ya lyofilize)

3. Harimo kugenzura ibyiza n'ibibi

4. Gutwara abantu ku bushyuhe busanzwe

5. Igikoresho kirashobora kuguma gihamye kugeza kumezi 18 kibitswe kuri -20 ℃.

6. CE yemeye

Urujya n'uruza:

1. Tegura RNA yakuwe muri SARS-CoV-2

2. Koresha neza RNA ukoresheje amazi

3. Tegura PCR ivanze

4. Koresha PCR master mix na RNA mugihe nyacyo cya plaque ya PCR cyangwa tube

5. Koresha igikoresho nyacyo-PCR