Waba uzi uburyo ibikoresho byihuta byo gukora bikora?

Immunology ni ingingo igoye ikubiyemo ubumenyi bwinshi bwumwuga. Iyi ngingo igamije kukumenyekanisha kubicuruzwa byacu ukoreshe imvugo ngufi yumvikana.

Mu rwego rwo gutahura byihuse, gukoresha urugo mubisanzwe ukoresha uburyo bwa zahabu ya colloidal.

Nanoparticles ya zahabu ihuzwa byoroshye na antibodies, peptide, oligonucleotide ya sintetike, hamwe nizindi poroteyine bitewe n’amatsinda ya sulfhydryl (-SH) ku buso bwa zahabu^3-5. Zahabu-biomolecule conjugate yinjijwe cyane mubikorwa byo gusuzuma, aho ibara ryumutuku ryera rikoreshwa murugo hamwe no kwipimisha-nko kwipimisha inda murugo

Kuberako ibikorwa byoroshye, ibisubizo biroroshye kubyumva, byoroshye, byihuse, byukuri nizindi mpamvu. Uburyo bwa zahabu ya colloidal nuburyo bukuru bwihuse bwo kumenya isoko.

Kurushanwa na sandwich byerekana ko aribintu 2 byingenzi muburyo bwa zahabu ya colloidal, Bakuruye inyungu kuberako imiterere yabakoresha ya gicuti, igihe gito cyo kugerageza, kwivanga gake, kugiciro gito, kandi byoroshye gukoreshwa nabakozi badasanzwe. Ubu buhanga bushingiye ku mikoranire ya biohimiki ya Hybridisation ya antigen-antibody. Ibicuruzwa byacu bigizwe n'ibice bine: icyitegererezo cy'icyitegererezo, ni agace kamanutseho urugero; conjugate pad, yanditseho tagi ihujwe nibintu biorecognition; reaction membrane irimo umurongo wikizamini n'umurongo wo kugenzura imikoranire ya antigen-antibody; na pisitori ikurura, ibika imyanda.

1.Soma Ihame

Antibodies ebyiri zihuza epitopes zitandukanye ziri kuri molekile ya virusi irakoreshwa. Imwe (coating antibody) yanditseho zahabu ya nanoparticles ya colloidal indi (gufata antibody) yashyizwe kumurongo wa NC membrane. Antibody yo gutwikira iri muburyo butagira umwuma muri padi ya conjugate. Iyo igisubizo gisanzwe cyangwa icyitegererezo byongewe kumurongo wicyitegererezo cyibizamini, binder irashobora guhita ishonga iyo ihuye numuyoboro wamazi urimo virusi. Hanyuma antibody yakoze urwego rugizwe na virusi mugice cyamazi hanyuma ikomeza imbere kugeza igihe ifashwe na antibody yashyizwe hejuru yimiterere ya NC membrane, itanga ikimenyetso mukigereranyo cya virusi. Byongeye kandi, antibody yinyongera yihariye ya antibody irashobora gukoreshwa mugutanga ibimenyetso byo kugenzura. Ikibaho cyinjiza kiri hejuru kugirango gitere capillarity ituma urwego rwumubiri rukururwa na antibody ihamye. Ibara rigaragara ryagaragaye mu minota itarenze 10, kandi ubukana bugena ingano ya virusi. Muyandi magambo, uko virusi nyinshi zagaragaye muri sample, niko byagaragaye umurongo utukura.

Reka nsobanure muri make uko ubu buryo bubiri bukora:

1.Uburyo bubiri bwo kurwanya sandwich

Uburyo bubiri bwa anti sandwich ihame, rikoreshwa cyane cyane mugutahura poroteyine nini yuburemere (anti) .Ibirwanya bibiri birasabwa kwibasira ahantu hatandukanye ka antigen.

2. Uburyo bwo guhatana

Uburyo bwo guhatana bivuga uburyo bwo gutahura antigen yashizweho n'umurongo wo gutahura hamwe na antibody yikimenyetso cya zahabu ya antigen igomba gupimwa.Ibisubizo byubu buryo birasomwa bitandukanye nibisubizo byuburyo bwa sandwich, hamwe nimwe umurongo mubyiza n'imirongo ibiri mubi.