Waba uzi uburyo ikizamini cyihuta cyihuta?

Umupfumu ni ingingo igoye ikubiyemo ubumenyi bwinshi bwumwuga. Iyi ngingo igamije kukumenyesha ibicuruzwa byacu gukoresha imvugo ngufi yumvikana.

Mu murima wo gutahura vuba, gukoresha urugo mubisanzwe ukoresha uburyo bwa zahabu ya Colloidal.

Zahabu Nanoparticles yahise ahunwa na antibodies, iragabanya, synthique oliponacleotide, nizindi poroteyine kubera ubuhinzi bwa sulfhydryl (-sh) amatsinda ya zahabu^3-5. Guhuza ibinyabuzima bya zahabu byinjijwe cyane mubikorwa byo gusuzuma, aho ibara ryabo ritukura rikoreshwa murugo no kwita ku kizamini cyo kwitaho nk'ibizamini byo mu rugo

Kuberako ibikorwa byoroshye, ibisubizo biroroshye kubyumva, byoroshye, byihuse, byukuri, byukuri nizindi. Uburyo bwa zahabu ya Colloidal nuburyo bwingenzi bwo kumenya byihuse kumasoko.

Amarushanwa na Sandwich assays ni moderi 2 nyamukuru mumiterere ya zahabu ya colloidal, bakurura inyungu kubera ibihe byabo byabakoresha, ibihe bigufi byinshuti, kwivanga gato, amafaranga make, no kuboroshezwa nabakozi badasanzwe. Ubu buhanga bushingiye ku mikoranire y'ibinyabuzima ya antigen-antibody hybridisation. Ibicuruzwa byacu bigizwe nibice bine: icyitegererezo cya padi, nicyo cyitegererezo cyarashenywe; conjugate padi, kuri po taque yanditseho ibinyabuzima bihuza; reaction membrane irimo umurongo wikizamini no kugenzura umurongo wa antigen-antibods; Kandi padi ikurura, ibangamira imyanda.

1.SasAy Ihame

Antibodies ebyiri zihuza epitopes zitandukanye zihari kuri virusi molekile ikoreshwa. Imwe (yambaye antibody) yanditseho zahabu ya Colloidal nanoparticles nandi (gufata antibody) yakosoye hejuru ya NC Membrane. Antibody ya Antibody iri muburyo bwo kurwara muri Conjugate Padi. Iyo igisubizo gisanzwe cyangwa icyitegererezo cyongeweho icyitegererezo cyikizamini cyikizamini, binder gishobora gushonga ako kanya nyuma yo guhura na virusi ikubiyemo virusi. Hanyuma antibodh yakoze ikigo cya virusi mu cyiciro cyamazi maze yimukira imbere kugeza igihe yafatiwe ku buso bwa NC Membrane, abantu benshi bibanda kuri virusi. Byongeye kandi, inyongera yinyongera yihariye ya antibody ya antibody irashobora gukoreshwa mu gutanga ikimenyetso cyo kugenzura. Pad ikurura iherereye hejuru kugirango iyobore na capiority ituma ubudahangarwa butuma antibody ihamye. Ibara rigaragara ryagaragaye munsi yiminota itarenze 10, kandi ubukana bugena ingano ya virusi. Mu rindi jambo, virusi myinshi yari ahari kurugero, bigaragara cyane ko bande itukura iragaragara.

Reka nsobanure muri make uburyo ubwo buryo bubiri bukora:

1.Uburyo bwo kurwanya Sandwich

Kabiri anti sandwich uburyo bwamahame, cyane cyane bikoreshwa mugutahura uburemere bunini bwa pelecular (anti) .TWo anti arasabwa kwibasira imbuga zitandukanye za antigen.

2. Uburyo bwo guhatana

Uburyo bwo guhatana bivuga uburyo bwo kumenyana na antigen umurongo wa antigen na antibody ya antimal ya antigen kugeragezwa. Ibisubizo byubu buryo burasomwa bitandukanye nuburyo bwa sandwich, hamwe numwe umurongo mumirongo myiza kandi ibiri mubibi.